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Citations for Millipore Sigma :
2401 - 2450 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Samples were stored at −20°C and insulin secretion was measured using a mouse insulin radioimmunoassay kit (Millipore Cat# HI-14K).
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bioRxiv - Plant Biology 2023Quote: ... All ATG3 recombinant proteins (wild-type and Cys-to-Ser mutants) were purified by affinity chromatography on a His-Select Nickel Affinity Gel (Sigma, P6611) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 10% input aliquots were taken and the rest of the sample was incubated with HIS-Select® Nickel Magnetic Agarose Beads (Sigma) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... was concentrated from the flow-through of the second passage through the His-trap column using an Amicon® Ultra Centrifugal Filter (Millipore) with a 30 kDa cut-off ...
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bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
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bioRxiv - Immunology 2023Quote: ... 293FT were exposed to supernatants containing pMXs-IRES-Blast-STING-His retroviruses and 4 μg/mL polybrene (Millipore, TR-1003-G). Two days post transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... the soluble fraction (obtained by centrifugation with 13000g at 4°C for 30 min) was applied to His-Select Nickel Affinity Gel (Sigma-Aldrich) in presence of 5 mM imidazole for 2h on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.8 mM KCl and 1.25 mM NaHCO3) before they were transferred to dissociation buffer [HBSS supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS, Sigma, F2442) and 10 mM HEPES (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich) were used on separate blots to detect PR3 ...
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bioRxiv - Biochemistry 2024Quote: ... Ubiquitin was expressed as an untagged protein and then purified and labelled with Cy3 as per ref53.TRIB1 C-terminus (345-372) was first purified using Ni2+ affinity resin (His-Select resin (Sigma-Aldrich) before being dialysed into PBS pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... placed in digestion media containing 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... cells were cultured for at least six days in RPMI 1640 Medium supplemented with 10% dialyzed FCS (Sigma Aldrich), containing either “heavy” lysine (40 g/l ...
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bioRxiv - Biophysics 2020Quote: ... Cytochalasin D (from Zygosporium masonii) as well as fetal calf serum (FCS) were from Sigma Aldrich (Merck KGaA, Germany). DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) ...
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bioRxiv - Molecular Biology 2021Quote: ... The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore). Purified proteins were observed by SDS gel migration as well as anti-human IgG Fc probing by Western ...
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bioRxiv - Developmental Biology 2019Quote: ... media was replaced with DMEM/F12 containing 100ng/ml Activin A and 0.2% foetal calf serum (FCS, Sigma-Aldrich) for a further 24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were grown in DMEM (gibco) with 1 g/mL glucose (MCEC) or 4.5 g/mL (HUVEC, HeLa, MCF-7) containing 10% FCS (Sigma), 1% penicillin (10000 Units/ml ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were seeded at 1E6 cells/ml in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma) and 50IU rhIL-2 (Peprotech).
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 cells were cultured in DMEM medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2 mM L-glutamine (Pan-Biotech ...
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bioRxiv - Biophysics 2020Quote: All used BMPs and extracellular domains (ECD) of the BMPR-FC chimeras were bought from Sigma Aldrich (Missouri, USA) and R&D systems (Minnesota ...
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bioRxiv - Cell Biology 2020Quote: ... the colon pieces were digested in RPMI-1640 medium containing 20% FCS and 100CU/mL collagenase IV (Sigma-Aldrich). Single cells were separated from the remaining tissue by filtration through 40 µm cell strainers and washed with RPMI-1640 thereafter ...
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bioRxiv - Synthetic Biology 2022Quote: ... were used in this work: The perfluorinated amine Fluorinert™ FC-40 was purchased from Sigma-Aldrich (Overijse, Belgium), while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel ...
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bioRxiv - Immunology 2020Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... cell pellets were resuspended with 10% FCS and fixed by adding an equal volume of 4% formaldehyde (Sigma-Aldrich) and mixed for 10 min at room temperature while shaking ...
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bioRxiv - Microbiology 2019Quote: ... washed cells were resuspended in incubation buffer (IB; Hank’s balanced salt solution, 2% dialysed FCS, 1x RPMI Amino Acids Solution (Sigma), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... Purified ACE2-Fc was concentrated and buffer exchanged into PBS by a 50-kDa MWCO ultra centrifugal filter (Millipore).
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bioRxiv - Immunology 2019Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... were cultured in Eagle’s Minimum Essential Medium (ATCC, Middlesex, UK) supplemented with 10% FCS and 0.8 mg/ml geneticin (Sigma-Aldrich). All cells were cultured at 37°C and 5% CO2 in humidified incubators and were free of mycoplasma contamination.
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bioRxiv - Immunology 2021Quote: ... cells were incubated in medium containing 2% FCS with or without the PAR2 negative allosteric modulator (AZ3451, Sigma Aldrich) or a NF-κβ inhibitor (Bay11-0782 ...
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bioRxiv - Microbiology 2020Quote: ... FMC Biopolymer (90) in DMEM (containing 2% FCS and antibiotics) and serial dilutions of 5-FU (F6627, Sigma-Aldrich) or Ribavirin (R9644 ...
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bioRxiv - Microbiology 2020Quote: ... The mice were boosted subcutaneously (s.c.) at day 8 with 50 μg of RBD-Fc plus Freund’s Adjuvant Complete (Sigma, USA) and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Digestion of the colon tissue was performed in RPMI/10% FCS containing 0.4mg/ml type VIII collagenase (Sigma Aldrich) and 40μg/ml DNase I (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Inocula were removed and replaced with DMEM containing 1% FCS and a semi-solid overlay consisting of 1.5% carboxymethyl cellulose (SIGMA). Cells were incubated for 72h ...
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bioRxiv - Cancer Biology 2024Quote: ... taken up in 40ml of FCS-free DMEM supplemented with 480 μl of 10 mg/ml Trypsin (Sigma, T9935) and 800 μl of 50 mg/ml Collagenase II (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... precoated with fibronectin-collagen IV (10 μg.ml-1) and grown in DMEM containing 10% Fetal Calf Serum (FCS) and 1 μM dexamethasone (Sigma) for 14 days to allow proper differentiation ...
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bioRxiv - Neuroscience 2024Quote: ... Forty-eight hours after transfection the medium with soluble RGMA-Fc or APP was concentrated through a 50,000 kDa column (YM-50, Millipore). The concentrated proteins were supplemented with Dulbecco’s modified Eagle’s medium 1 g/L glucose (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Genomics 2024Quote: ... at a density of ∼2.5*106 cells/mL in 5% fetal calf serum (FCS) (Bodinco BDC, 16941) DMEM (Dulbecco’s Modified Eagle’s Serum (Sigma, 05796), 1% Pen-Strep (Lonza ...
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bioRxiv - Genomics 2024Quote: ... at a density of ∼2.5*106 cells/mL in 5% fetal calf serum (FCS) (Bodinco BDC, 16941) DMEM (Dulbecco’s Modified Eagle’s Serum (Sigma, 05796), 1% Pen-Strep (Lonza ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed in RPMI supplemented with 10% FCS and incubated with 10 μM of MG132 (Sigma Aldrich) for 3h ...
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bioRxiv - Microbiology 2023Quote: ... Parasites containing restored S14 loci were selected by negative selection using a 5-fluorocytosine (5-FC) drug (Sigma, USA) as previously described (Srivastava & Mishra ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were frozen at 1 x 107 cells per 1 ml of freezing medium (FCS, 10 % DMSO (Sigma-Aldrich)) ...
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bioRxiv - Genetics 2023Quote: ... in supplemented RPMI1640 medium (10% heat-inactivated FCS, 2mM L-glutamine, 50IU/ml penicillin, 50μg/ml streptomycin; Sigma-Aldrich), 1×106 MLN cells/well were stimulated by 50ng/ml PMA and 1μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Eurobio Scientific, CVFSVF00-01) and containing 1% penicillin-streptomycin (Sigma-Aldrich, P4333). HEK-293T were grown in Hepes-modified DMEM (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... and digested in 20 mL of DMEM added with 20% FCS and 100 U/ml of collagenase (Sigma-Aldrich) for 40 min at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... anti-p-Histone H2A.X Ser139 (ψH2A.X, mouse monoclonal, 05-636) and pan-ADP-ribose binding reagent (rabbit Fc tag, MABE1016) were obtained from Millipore. anti-DHFR (rabbit polyclonal ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...