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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was detected using FITC labeled VAD-FMK molecule to detect activated caspases (Sigma-Aldrich), or by Annexin V-APC staining (Ebioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 µL of fluorescein-labeled dextran stock solution (Sigma, 20 mg/mL in 0.9% NaCl) and 50 µL of Dylight 648-labeled Isolectin GS-B4 (Nordic-MUbio ...
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bioRxiv - Bioengineering 2023Quote: ... 20 μg ml-1 AlexaFluor 488-labeled peanut (Arachis hypogaea) agglutinin lectin (PNA, L21409, Sigma), and/or 1:40 AlexaFluor 647 phalloidin (A22287 ...
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bioRxiv - Bioengineering 2024Quote: ... Apoptotic Jurkat cells (ACs) were then labeled with the tracking dye PKH67 (Sigma-Aldrich, #MIDI67), and any excess dye was removed thorough two washes with 1% BSA in HBSS (WELGENE ...
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bioRxiv - Immunology 2023Quote: ... dialyzed against carbonate-bicarbonate buffer (pH 9.0) and labeled with fluorescein isothiocyanate (FITC, Sigma F3651). The fluorescein-labeled antibody was separated from the unconjugated antibodies by Sephadex G-25M size exclusion chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... Labeled sections were mounted on gelatin-coated slides (1% bovine gelatin; Cat#G9391, Sigma-Aldrich) and dried overnight at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Released glycans were fluorescently labeled with 8- aminopyrene-1,3,6-trisulfonic acid (APTS; Sigma-Aldrich, Germany) and excess of APTS and agents were removed by hydrophilic interaction liquid chromatography-solid phase extraction (HILIC-SPE) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by detection with fluorescently labeled secondary antibodies were used: anti-mouse CF680 (Sigma #SAB460199), anti-rabbit CF770 (Sigma #SAB460215) ...
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bioRxiv - Cell Biology 2023Quote: ... the first round of dying cells was labeled with CellVue Claret Far Red (Millipore Sigma) and co-cultured with macrophages at a 1:1 ratio for 45min.
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bioRxiv - Systems Biology 2023Quote: ... The sections were subsequently stained with fluorescein isothiocyanate (FITC)-labeled wheat-germ-agglutinin (WGA; Sigma) to visualize and quantify the cell cross-sectional area ...
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bioRxiv - Microbiology 2023Quote: Macrophages were labeled with the fluorescent dye PKH-26 (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then labeled using primary mouse anti-vinculin antibody (1:100; Sigma-Aldrich) and donkey anti-mouse Alexa Fluor 555-conjugated secondary antibody (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... DNA content was labeled by incubating cells in 1 µg/mL DAPI (Sigma-Aldrich Chemistry) supplemented with 100 µg/mL RNase (Sigma-Aldrich Chemistry ...
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bioRxiv - Neuroscience 2024Quote: Neurons were labeled using the anti-NeuN Antibody (clone A60, MAB377 Sigma-Aldrich, Host-mouse) as the primary antibody and Cy3 (Donkey anti-mouse IgG ...
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bioRxiv - Microbiology 2024Quote: ... The horseradish peroxidase (HRP)-labeled-Goat anti-Mouse or Goat anti-Rabbit IgG (Sigma, USA) was used as secondary Ab ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... the mice were double labeled with subcutaneous injections of 15 mg/kg of calcein (Sigma) at 4 and 2 days before sacrifice ...
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bioRxiv - Cancer Biology 2021Quote: Anti-Flag (Cat. number: F7425) and anti-His (Cat. number: SAB1306085) antibodies were from Sigma-Aldrich (St. Louis, MS, USA). Anti-phospho-γ-H2AX (Ser139 ...
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... Y187 strains were grown on SD/-Trp-Leu medium and transferred to SD/-Trp-Leu-His medium with 10 mM 3-aminotriazole (3-AT, Sigma) in gradient dilutions (1:10 ...
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
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bioRxiv - Biophysics 2021Quote: The bacteriophage T7 gp16 gene was cloned into the pET-16b vector (ampr and 10x-His tag) from Novagen (Germany), inserted between NdeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2020Quote: ... Trx-His(6)-hSLAP2 or Trx-His(6)-Δlinker-hSLAP2 were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Plant Biology 2021Quote: ... The cell lysate was centrifuged for 30 minutes at 5,000 x g and the supernatant was recovered and mixed with HIS-Select Nickel Affinity Gel (Sigma Aldrich) for protein purification (manufacturer’s instructions were followed) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence was excised with BamHI/EcoRI and ligated downstream of the His tag into the multiple cloning site MCS1 of pETDuet-1 (ampicillin resistance; Novagen), yielding Sll0171-pETDuet-1 ...
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bioRxiv - Biochemistry 2022Quote: ... the protein sequence fused to an N-terminal GB1 solubility tag and a 6x TEV-cleavable His-tag was cloned into pET24b (Novagen). Plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Cell Biology 2020Quote: ... bacterial pellet was resuspended in 20 ml of cold His-lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0) with protease inhibitor (Sigma-Aldrich), followed by sonication (10 x 20-second pulses) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed 4 x 5 min with TBS and the bound SadP was detected with 1:10000 dilution of anti-His primary antibody (Sigma) and 1:20000 dilution of HRP-labelled secondary rabbit anti-mouse antibody (DakoCytomation) ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Germany) were precoated with murine anti-HIS mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... This was followed by four washes with 200 µl of incubation buffer and incubation with 50 µl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at 24°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cerevisiae were cloned into 10×His-MBP-SUMO-nup-SNAP constructs (Figure 2A) via a pET-28a-derived vector (Novagen), and expressed in E ...
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bioRxiv - Microbiology 2019Quote: ... and the PCR product was purified and cloned in-frame with a His-tag into the IPTG-inducible expression plasmid pET-15b (Novagen) to create construct pET15b-Pnf ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was then transferred to a 15 mL tube and incubated with 1 mL His bead slurry (Sigma-Aldrich; P6611) for 20 minutes at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma] ...
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bioRxiv - Biochemistry 2020Quote: ... Samples corresponding to 1.4 OD600 of cells were resolved over SDS-Western blot was developed using anti-His specific antibody (Cat no. Sigma:H1029) at a dilution of 1:3000 and HRP conjugated anti-mouse secondary antibody (Anti mouse IgG-A4416 ...
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bioRxiv - Microbiology 2020Quote: ... was cloned as 10× His-MBP-SUMO-NUP153 (or NSP1)-SNAP constructs (Figure S1) into a pET-28a-derived vector (Novagen) and expressed in BL21-Gold (DE3) ...
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bioRxiv - Molecular Biology 2020Quote: ... Trx-His(6)-Δlinker-hSLAP2 or Trx-His(6)-mSLAP were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% (v/v) Tween 20) containing 5% (w/v) BSA for 1 hour and then incubated with anti-His (Sigma) or anti-GFP antibody (Clontech ...
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bioRxiv - Cell Biology 2024Quote: ... The filtered lysate was then incubated for 30 min with 1 ml of equilibrated HIS-Select HF Ni-affinity gel (Sigma) and loaded onto a gravity flow column ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant supplemented with final 10 mM imidazole was incubated with His-Select Nickel Affinity Gel (P6611, Lot No. 110M5166, SIGMA) for 1.5 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked using 5% nonfat milk and probed with anti-poly-His primary antibody (cat. no. H1029; Sigma-Aldrich) at 1:3,000 dilution or anti- GST primary antibody (cat ...