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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for Human High Sensitive Leucine Rich Alpha 2 Glycoprotein 1 LRG1 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... PTU (1-Phenyl-2-thiourea) was purchased from Sigma (Sigma, Zwijndrecht, the Netherlands). BODIPY™ 581/591 C11 was purchased from Thermo scientific (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM EGTA) with 2 x Protease Inhibitor Cocktail (Millipore Sigma cOmplete™) on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Zebrafish embryos were treated with 0.003% 1-phenyl-2-thiourea (PTU, Sigma P7629) starting at 24hpf to prevent pigmentation.
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bioRxiv - Genetics 2023Quote: ... Cells were fixed with a 1:10 dilution of 2% glutaraldehyde (Sigma Aldrich) in 0.2 M phosphate buffer pH 7.5 (0.31 g NaH2PO4 and 1.09 g Na2HPO4 in 50 ml water) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM MgCl2 and 2 mM CaCl2 (all chemicals were from Sigma-Aldrich) at the flow rate of 10 ml/min for 2 min to remove blood ...
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bioRxiv - Neuroscience 2023Quote: ... for 2h before clearing in benzyl alcohol:benzyl benzoate (1:2) (Sigma-Aldrich-Merck). Samples were stored in this solution at room temperature and protected from light.
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bioRxiv - Bioengineering 2023Quote: ... 1-2% (v/v) Span® 80 surfactant69 (Sigma Aldrich, St. Louis, MO) was spiked into mineral oil (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... each step supplemented with phosphatase inhibitor cocktail 2 (1:100, Sigma, cat#P5726). Samples were mounted in DABCO anti-fade medium prior imaging.
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bioRxiv - Molecular Biology 2023Quote: ... 2 to 3 million cells were cross-linked using 1% formaldehyde (Sigma, F8775) for 10 minutes at room temperature ...
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bioRxiv - Genetics 2023Quote: ... incubated with 4′,6-Diamidino-2-phenylindole dihydrochloride (1:10,000; Millipore Sigma, D8417) in wash buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 2% charcoal-stripped FBS and EPC cocktail (1 µM MPA, Sigma Cat#1378001- 200MG ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044). Lysates were cleared at 17,000g ...
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bioRxiv - Cell Biology 2024Quote: ... and 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI, 1/3000, Sigma-Aldrich, UK) for 1 hour at room temperature protected from ambient light ...
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bioRxiv - Cell Biology 2024Quote: ... fracture tissues were digested with 1mg/ml Collagenase 1/Collagenase 2 (Sigma-Aldrich) and 2 mg/ml dispase II (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:500 dilution + 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) at a 1:1000 dilution.
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bioRxiv - Physiology 2024Quote: ... Liver (50mg) was extracted in 2:1 Chloroform: Methanol mixture (Chloroform: Sigma Aldrich, C2432-1L ...
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bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
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bioRxiv - Cell Biology 2023Quote: ... GLP-1 levels were measured using a total GLP-1 ELISA kit (Millipore) and an Infinite 200 Pro Reader (TECAN ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mM NaF, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 1 mM PMSF, 75 mU/ml aprotinin-Sigma-, 50 mM Tris-HCl, pH 7.5) containing 1% TX-100 ...
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bioRxiv - Immunology 2020Quote: ... plasma samples were incubated at a dilution of 1:100 and bound antibodies were detected with goat anti-human IgM/HRP (Sigma: cat. A6907, 1:6’000 dilution), goat anti-human IgG/HRP (Thermo Fisher ...
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bioRxiv - Physiology 2022Quote: ... The initial culture medium was changed at passage 1 to Dulbecco’s Modified Eagle’s Medium-high glucose (DMEM, Sigma-Aldrich, Switzerland), with 10 % FBS ...
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bioRxiv - Cell Biology 2021Quote: ... 1% penicillin-streptomycin and 10% FBS in Dulbecco’s modified Eagle’s medium/Ham’s-High Glucose (DMEM-HG) (Sigma-Aldrich, Shanghai, China). The medium is replacing it with a fresh one every two days.
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bioRxiv - Cell Biology 2021Quote: ... The HEK 293 cells were cultured in DMEM high glucose supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (complete medium; all from Sigma). Stable expression of the constructs in the HEK 293 cells was maintained by selection with hygromycin B (250 μg/ml final ...
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bioRxiv - Molecular Biology 2021Quote: ... were seeded at 1×106 cells per 10 cm culturing dish in 15 ml media (DMEM High Glucose, 10% Tet-Free FBS (Sigma), 1% PenStrep ...
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bioRxiv - Neuroscience 2021Quote: ... frozen VMH (from 2 – 3 mice) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... the antibodies isotypes were determined by ELISA using a mouse Ig isotyping kit (ISO-2, SIGMA).
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Microbiology 2020Quote: ... Strains were routinely grown under yeast conditions (media at 30°C) in YPG (1% yeast extract, 2% Bacto peptone, 2% glucose) or under filament-inducing conditions using RPMI medium (Sigma, St. Louis, MO) with MOPS (morpholinepropanesulfonic acid ...
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bioRxiv - Plant Biology 2021Quote: ... total soluble protein was extracted from 50 mg of ground frozen shoot material in 250 µl 2× Laemmli buffer supplemented with 1% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich, Steinheim, Germany). Proteins were denatured for 10 min at 90°C and separated using 10% (w/v ...
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bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were infected at low MOI 1-2 in EBM-2 media with supplements in the presence of 8 μg/mL polybrene (Sigma-Aldrich, St.Louis, USA) and spinoculation at 450g for 30 min ...
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bioRxiv - Bioengineering 2020Quote: ... 2-hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 410896, Sigma Aldrich), VEGF specific control aptamer (47-nt ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Biophysics 2022Quote: ... the pancreas was transferred to a foil-covered glass vial containing a 1:1 solution of MeOH (100%) and BABB (1:2 (v/v) benzyl alcohol (402834-500ml, Sigma-Aldrich, Gillingham, UK) to benzyl benzoate (B6630-1L ...
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bioRxiv - Cancer Biology 2023Quote: ... CRISPR CCR5 locus insert cell lines were grown in Dulbecco’s Modified Essential Medium High Glucose (DMEM-high Glucose) (Sigma-Aldrich) with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated in sucrose buffer (0.1 M Tris-HCl pH = 7.5, 2 mM MgCl2, 1 M sucrose, 1 % Protease Inhibitor Cocktail (Sigma Aldrich), 200 μg.mL−1 RNase A and 10 μg.mL−1 DNase (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Neuroscience 2021Quote: ... and 2 % of FBS for 1 h at room temperature (RT) and incubated with PSD95 (1:1.000, MAB1598; Millipore), PrP (1:500 ...
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bioRxiv - Genomics 2022Quote: ... G418 foci were washed with 1× PBS and fixed using 2% Formaldehyde/0.2% Glutaraldehyde in 1× PBS (Sigma-Aldrich) fixing solution at room temperature for 30min ...
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bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Percoll gradients (1-2 per sample depending on harvest density) were formed by vortexing 1 ml Percoll (Sigma P1644) with 110 µl 10x PBS in 2 ml tubes and centrifuging 15 min at 15,000 g ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in the primary antibody (anti-Kv3.2 antibody, 1:200, Cat.no. APC-011, alomone lab, Israel; anti-GAD67 antibody, 1:200, Cat.no. MAB5406, Sigma, USA) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1% Phosphatase inhibitors cocktail 2 Sigma-Aldrich Cat:P5726 ...