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Citations for Millipore Sigma :
2401 - 2450 of 4804 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Physiology 2023Quote: ... Samples were stored at −20°C and insulin secretion was measured using a mouse insulin radioimmunoassay kit (Millipore Cat# HI-14K).
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bioRxiv - Neuroscience 2024Quote: ... The tissues were mashed through a 70-μm cell strainer in PBS / 2% heat-inactivated fetal bovine serum (HI-FBS; Sigma-Aldrich) / 10 mM EDTA-Na (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich) were used on separate blots to detect PR3 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.8 mM KCl and 1.25 mM NaHCO3) before they were transferred to dissociation buffer [HBSS supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS, Sigma, F2442) and 10 mM HEPES (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... the soluble fraction (obtained by centrifugation with 13000g at 4°C for 30 min) was applied to His-Select Nickel Affinity Gel (Sigma-Aldrich) in presence of 5 mM imidazole for 2h on ice ...
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bioRxiv - Immunology 2022Quote: ... To prepare HI-WCA-OT-109 copies/ml of bacterial suspension was made up in R10 media: RPMI 1640 (Sigma, R0883) containing 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... 293FT were exposed to supernatants containing pMXs-IRES-Blast-STING-His retroviruses and 4 μg/mL polybrene (Millipore, TR-1003-G). Two days post transduction ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Microbiology 2024Quote: ... CrsL-His proteins were loaded in duplicate and resolved on SDS-PAGE gels and blotted onto Amersham Protran Nitrocellulose Membrane (Sigma-Aldrich). The membranes were incubated with decreasing concentrations of guanidine-HCl (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: pDest17 plasmids containing an N-terminal 6x-His tag and Flag-tagged ARH3 wildtype or H182R were transformed into Rosetta (DE3)pLysS Competent Cells (Sigma, #70956). An initial 5 mL culture was grown overnight in Lennox LB broth supplemented with 100 μg/mL of ampicillin and 25 μg/mL of chloramphenicol at 37 °C while shaking at 200 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... Ubiquitin was expressed as an untagged protein and then purified and labelled with Cy3 as per ref53.TRIB1 C-terminus (345-372) was first purified using Ni2+ affinity resin (His-Select resin (Sigma-Aldrich) before being dialysed into PBS pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: AdfN and AdfN(K43A) were expressed from pET vectors with a 6x-His tag in Rosetta 2 (DE3) cells (Millipore Sigma). For expression ...
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bioRxiv - Cell Biology 2024Quote: 200 µg of freshly eluted CD95L-IZ-His was reduced with 5 mM dithiothreitol (DTT) and incubated with 13 µg (1:15 w/w) TEV protease (Sigma-Aldrich) at 4 °C overnight ...
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bioRxiv - Biophysics 2024Quote: ... and H3.1 were subcloned into a pET15b-derived plasmid containing an N-terminal 6×His tag (MGSSHHHHHHSSG) in which the thrombin cleavage site of pET15b (Merck Millipore, 69661) was replaced by the TEV protease recognition sequence ...
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bioRxiv - Biochemistry 2022Quote: ... λ emission 405 nm) and were quantified with the Waters Empower Pro software using authentic standard compounds (SA sodium salt and GA, Sigma–Aldrich, Madrid, Spain). Standard curves were performed for each compound using similar concentration ranges to those detected in the samples ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then clarified by centrifugation and the protein content of each supernatant was determined using the Quanti-Pro bicinchoninic acid (BCA) assay kit (Sigma-Aldrich, MO, USA). Equal protein amounts (100 μg ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Immunology 2023Quote: ... Lung tissue was homogenized using a handheld homogenizer (Pro Scientific) and RNA was isolated via standard trizol/chloroform (Invitrogen Life Technologies and Sigma Aldrich, respectively) extraction per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... n-dodecyl β-D-maltoside (DDM, Sigma-Aldrich, St. Louis, MO), n-octylglucoside (OG ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 10 μg/ml Actinomycin D (Sigma-Aldrich) for the indicated time periods and then harvested for RT-qPCR analysis ...
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bioRxiv - Cell Biology 2021Quote: ... treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich, St. Louis, MO). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... passaged onto Poly-D-Lysine (50 µg/mL, Sigma-Aldrich, P1024) and Laminin (5 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Freshly isolated MuSCs were then plated onto poly-D-lysine (Millipore) and ECM (Sigma E1270 ...
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bioRxiv - Immunology 2021Quote: ... cells were loaded with Fluorescein di(β-D-galactopyranoside) (FDG, Sigma). Single cell suspensions in warm HBSS buffer (1x HBSS supplemented with 2% fetal calf serum and 10mM buffer [pH 7.2] ...
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bioRxiv - Developmental Biology 2021Quote: ... and embryos collected in M2 media (MR-015-D, Sigma-Aldrich) at indicated time points after hCG injection ...
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bioRxiv - Plant Biology 2020Quote: ... 0.3 mM CaCl2 and 20 mM D-sorbitol (Sigma cat# 8143). Upon preparation ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 coverslips coated with poly-D-lysine hydrobromide (Sigma-Aldrich, #P6407). Cells in each well were transiently transfected using 100 ng V2R and 900 ng sheared salmon sperm DNA (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 50 µl DNaseI [13K U/ml] (Sigma D-4527, 40K Units), and 50 µl L-cysteine [152.2 mM] (Sigma C-1276) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4-Methylumbelliferyl β-D-glucopyranoside (Sigma-Aldrich, St. Louis, MO, USA), normocin (InvivoGen ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 2 μg/ml Actinomycin D (Sigma-Aldrich) or DMSO as a control and collected at indicated time points ...
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bioRxiv - Neuroscience 2021Quote: ... spotted in dense culture spots (20uL) on poly-D-lysine (Sigma) and laminin coated chambered coverglass (Nunc ...
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bioRxiv - Immunology 2022Quote: ... p-Nitrophenyl-N-acetyl-β-D-glucosaminide (pNAG) was from Sigma. The IRAP inhibitors 4u and 11b were a gift from E.Stratikos (Demokritos Research Center Athens).
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... The transfected cells were harvested in cold D-PBS (Sigma-Aldrich) after 2 days ...
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bioRxiv - Genomics 2020Quote: ... were coated overnight with 50 μg/mL poly-D-lysine (Sigma) in 0.1M borate buffer (pH 8.5) ...
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bioRxiv - Immunology 2021Quote: ... cells were treated with actinomycin D (5μg/ml, Sigma, Cat. A9415) for the indicated time ...
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bioRxiv - Cancer Biology 2021Quote: ... and actinomycin D (A1410) were purchased from Sigma (St. Louis, MO). G418 (4727878001 ...
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bioRxiv - Microbiology 2021Quote: ... oligomycin A and 2-deoxy-D-glucose were purchased from Sigma. Primary antibodies include mouse mAb DG52 anti-SAG1 (provided by John Boothroyd) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... d-ACC (1-Aminocyclopropane-2,2,3,3-d4-carboxylic acid; Sigma cat#736260), d-IAA([2H5] indole-3-acetic acid ...
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bioRxiv - Biochemistry 2020Quote: ... 3.5 g/L 13C,2H D-Glucose (Sigma-Aldrich Cat # 552151) and 1g/L 15NH4Cl (Sigma-Aldrich Cat # 299251) ...
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bioRxiv - Neuroscience 2020Quote: ... and 4.5 g/L D-glucose (Millipore-Sigma, Burlington, MA, USA). To wells containing 200 µL DPBS-glucose solution ...
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bioRxiv - Neuroscience 2020Quote: ... and 4.5 g/L D-glucose (Millipore-Sigma, Burlington, MA, USA). To wells containing 200 µL DPBS-glucose solution ...
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bioRxiv - Neuroscience 2020Quote: ... Following intraperitoneal (i.p.) administration of d-amphetamine (2.5 mg/kg, Sigma), mice were placed in a transparent beaker with a diameter of 11.5 cm ...
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bioRxiv - Neuroscience 2020Quote: ... pre-coated with poly-D-Lysine (0.1 mg mL-1; Sigma). Neurons (600,000 cells per 60-mm dish or 80,000 cells per 12-mm coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... DRG were then plated onto poly-d-lysine/collagen (Sigma-Aldrich)-coated 12-mm glass coverslips (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... which was pre-coated with 0.1 % Poly-D-lysine (Sigma P6407) and 0.1 % adhesion proteins laminin (Sigma L2020 ...
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bioRxiv - Systems Biology 2021Quote: ... and 20 g l−1 D-glucose (Sigma-Aldrich Co., Germany) for the 100mM (2% ...
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bioRxiv - Plant Biology 2021Quote: ... Fourty millimolar stock solutions of shikimate and D-quinate (Sigma-Aldrich) were prepared in H2O ...