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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for Canine Adenovirus 1 and 2 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... then placed in a 1.7 mL microcentrifuge tube with 1 mL of BY-2 media containing either 0.05% trypsin (Sigma T2601), 0.8% cellulase (Duchefa Biochemie C8001) ...
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bioRxiv - Cell Biology 2020Quote: ... MitoTEMPO ((2-(2,2,6,6-Tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl)triphenylphosphonium chloride; #SML0737) and KCN (potassium cyanide; #60178) from Sigma-Aldrich; chemicals applied in Saccharomyces cerevisiae Meyen ex E.C ...
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bioRxiv - Bioengineering 2020Quote: ... according to the manufacturer’s protocol and the cDNA was diluted 1:2 in molecular biology grade water (Sigma,UK). Gene expression analysis was performed by quantitative real time PCR on a RotorGene 6000 cycler (Corbett Research ...
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bioRxiv - Biophysics 2020Quote: ... 10 μL was removed and pulsed into a tube containing 1 μL of 2 mg/mL thermolysin (Sigma Aldrich) for 1 min before quenching with 3 μL 500 mM EDTA ...
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bioRxiv - Microbiology 2021Quote: ... After incubation at room temperature for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Cell Biology 2021Quote: ... Then the gel was washed twice with PBS and coated with 2 ml of 0.2 mg ml−1 rat tail collagen type I (Millipore) at 4 °C overnight.
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bioRxiv - Immunology 2021Quote: ... 0.05μg/mL IL-2 and activated with cell stimulation cocktail (eBioscience) for 1 hour prior to adding 10μg/ml brefeldin A (Sigma) and culturing for a further 4 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Immunology 2022Quote: ... samples were re-loaded four times before washing with 1 mL of 2% acetonitrile (ACN) (#1000292500, Sigma-Aldrich, Merck) in 0.2% acetic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μL droplets containing a total of 1000 cancer and stellate cells in a 1:2 ratio were prepared in a 0.24% solution of methylcellulose (M0512, Sigma) and plated on the underside of a tissue culture plate lid ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
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bioRxiv - Molecular Biology 2022Quote: ... and pellets were resuspended in NP40 buffer (20 mM Tris pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% NP40) and treated with Benzonase (Sigma). Anti-HA magnetic beads (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... and 80% Buffer-Z (0.1 M Na2HPO4, 10 mM KCl, 1 mM MgSO4, and 3.4 µL/mL 2-mercaptoethanol; all from Sigma-Aldrich). Luminescence and absorbance (410 nm ...
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bioRxiv - Plant Biology 2022Quote: ... as well as commercially available mouse anti-alpha-tubulin (Sigma-Aldrich, USA, cat. no. T9026-2, dilution 1:300), rabbit anti-histone H3K4me2 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl plasmid vectors (2 μg) and/or siRNA oligos (0.1 nmol) containing 0.05% fast green FCF (Sigma-Aldrich) were injected into each DRG ...
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bioRxiv - Biochemistry 2022Quote: Phagosomal proteins from RAW264.7 cells were extracted in 1% sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate (commercially available as RapiGest, Waters) in 50 mM Tris (Sigma) pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... The agarose pad was made by mixing 0.5 ml 2% agarose Optiprep mixture (20 mg agarose in 1 ml Optiprep (Sigma), heated to 90 °C ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2022Quote: ... SDS lysate was centrifuged at 20,000 x g for 30 minutes and supernatants were subsequently diluted with 7 volumes of 1.15% Triton-X 100 in TNE with Phosphatase Inhibitor Cocktail 1 and 2 (Sigma) to produce a mixed-micelle lysate with final detergent concentrations of 0.125% SDS and 1% Triton-X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... DNA plasmids were used at 2 µg/µl and mixed with 1% fast green (Sigma-Aldrich, final concentration 0.2%). Plasmids were injected into the ventricle with a pump-controlled micropipette ...
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bioRxiv - Plant Biology 2022Quote: ... were also amplified by PCR using the primers listed in Supplemental Table 2 and cloned into pGEX4T-1 (Novagen) using BamHI/EcoRI sites to N-terminal glutathione S-transferase (GST ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was re-extracted with 450 µL methanol:water (2:1, v/v) containing 1nmol of scyllo-inositol (Sigma) as an internal standard ...
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bioRxiv - Microbiology 2023Quote: Mtb WT and virRmut strains were grown in 7H9 media supplemented with ADC and Tween 80 to an OD 540 of 0.2 and then diluted to an OD540 of 0.1 with 7H9 containing 0 or 50 µgml-1 lysozyme (Sigma). The cultures were incubated with agitation at 37°C and OD540 was monitored daily.
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bioRxiv - Physiology 2024Quote: ... washed 2 x 5 min in PBS and then incubated with primary antibodies against Laminin-β1γ1 (1:200; #L9393, Sigma) in 3% BSA in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted in 1% normal donkey serum in PBS with 2 μg/ml DAPI (Sigma-Aldrich, St. Louis, MO, USA) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: Lhx6-Cre;Ppargc1a+/+ and Lhx6-Cre;Ppargc1aF/F animals were injected at P1 with 600nL of custom-made AAV8-hSyn-dio-mito-EmGFP (diluted 1:2 with sterile PBS and 0.5% Fast Green, Sigma). The Vanderhaeghen lab gifted the mito-EmGFP construct ...
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bioRxiv - Neuroscience 2024Quote: ... mixed with AAV8-hSyn-dio-GFP (Addgene #50457-AAV8, 1:2 dilution with sterile PBS and 0.5% Fast Green (Sigma)) ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:40 dilution for sparse cell targeting and AAV8-hSyn-dio-GFP (Addgene #50457-AAV8, 1:2 dilution with sterile PBS and 0.5% Fast Green, Sigma). The dilution factor of the DREADDs viruses (here 1:40 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were treated with mitoDFX (0.03-1 µM) in the absence or presence of 2-DG (50 mM; Sigma) or oligomycin (2 µM ...
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bioRxiv - Immunology 2024Quote: ... cell nuclei were stained with 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, Cat. No. D9542) diluted in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... homogenised in 6 mL of 2:1 (v/v) chloroform:methanol containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma-Aldrich) as antioxidant ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipids were resuspended in chloroform: methanol (2:1 v/v) and loaded onto a silica TLC plate (Sigma 60778). For nonpolar lipid separation ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden were cut into 6x6 mm squares and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Plant Biology 2023Quote: ... washed three times in sterilised water and sown on half-strength Murashige and Skoog media (1/2 MS) (Sigma), 3 mM MES-KOH (pH 5.7) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 50mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Synthetic Biology 2023Quote: ... A total of 1,000 µL transduction mix containing 1 µL of 2 µg/mL Polybrene (Sigma-Aldrich #TR-1003), recombinant lentivirus ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS plus 0.1% triton (PBST) for 2 hours and then transferred to 1:1000 TH anti-rabbit primary antibody (Millipore) with 1% NDS in PBST overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the slides were washed with TBS and incubated with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were finally washed three times and incubated in 4,6-diamidino-2-phenylindole (DAPI) (1 µg/ml; Sigma-Aldrich) for 5 min in dark ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were applied for 2 hours at room temperature and counterstained with DAPI (Sigma Aldrich D9542, 1:10000) for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Intestinal tissues were then finely chopped and digested in complete HBSS-2 (HBSS, 25 mM HEPES, 1 mM sodium pyruvate) containing 0.05 mg/mL Collagenase VIII (Sigma) and 50 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... digestion was performed at an enzyme:protein ratio of 1:100 for 2 hours at 25°C followed by trypsin (Sigma) digestion at an enzyme:protein ratio of 1:50 overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... weighted and incubated in 2 mL lysis solution consisting of 1% v/v Triton-X-100 (108603, Sigma-Aldrich) + 5 mM MgCl2 (M2393 ...
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bioRxiv - Immunology 2023Quote: ... lavage fluids (1/2 diluted; 100 µl) were incubated with 500 µM Gly-Pro-p-nitroanilide substrate (Sigma-Aldrich) in 200 mM Tris-HCl buffer (pH 8.3 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.4) and adjusted to an OD600 of 1.0 in HEPES buffer containing either 1 mM Aldrithriol-2 (Sigma-Aldrich, Darmstadt ...
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bioRxiv - Bioengineering 2022Quote: A mixed solution containing 2 μL/mL of Ethd-1 and 0.5 μL/mL of Calcein AM (Sigma-Aldrich) was prepared ...
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bioRxiv - Developmental Biology 2023Quote: ... The dried residues were reconstituted in 50 µL of 2% MeOX in anhydrous pyridine (110-86-1, Sigma-Aldrich) followed by derivatization at 70°C for 2 hours ...