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Citations for Millipore Sigma :
2401 - 2450 of 2589 citations for Apolipoprotein C2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The recombinant xDnmt1 was eluted twice in 250 µL EB containing 1 mM DTT and 250 µg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare Biosciences).
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated with 1 µg/ml of gp33 peptide in the presence of 10 µg/ml Brefeldin A (Sigma-Aldrich) at 37°C for 6 h ...
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bioRxiv - Immunology 2022Quote: ... The proteins captured on the anti-FLAG resin were eluted by incubation with 46 μl of TBS containing 150 ng/µL of 3X FLAG peptide (Sigma, #F4799). For mass spectrometry (MS ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were washed two times with 200 µl 1% TFA 99% ethyl acetate (ThermoFisher Scientific; Cat#: 28904; Millipore Sigma; Cat#: 270989) followed 200 µl 0.2% TFA/5%ACN (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The desalted peptides in the cartridge were eluted using a buffer containing 0.5% acetic acid and 80% acetonitrile (Sigma-Aldrich-Aldrich). The samples were subsequently dried using a speedvac for mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... numbering includes the signal peptide) with C-terminal Flag and 6x-Histidine tags and subsequently cloned into a p32A vector (Millipore Sigma). The TrxA-3C-ligand-Flag-6xHis construct was transformed into Origami E ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg of biotinylated peptides were bound to 25-50 μl of a 50:50 slurry of streptavidin agarose (Sigma-Aldrich), mixed with recombinant proteins or cell lysates ...
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bioRxiv - Neuroscience 2022Quote: Seven-week isogenic control- and healthy control hiPSC derived neurons were treated with 2.5 μM Aβ peptide 1-42 (Sigma, PP69-0.25MG) for 24 hours.
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... Proteins were eluted by rotating the resin at room temperature for 1 hour in 100 μL of PBS containing 150 ng/ μL 3×-FLAG peptide (Sigma Aldrich). 100 μL of 2x sample loading buffer was added to the eluate and samples were boiled at 95 °C for 10 minutes ...
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bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
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bioRxiv - Genetics 2023Quote: Liver protein isolation and western blots on liver extracts and plasma samples were performed as previously described.17 Primary antibodies to the 2A peptide (1:5000, rabbit, ABS31, Sigma-Aldrich), GFP (1:3000 ...
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bioRxiv - Biochemistry 2023Quote: ... the affinity columns were eluted with 500ul of 0.5ug/ul FLAG peptide prepared according to the manufacturer instruction (Sigma-Aldrich #F3290).
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated with 10nM SIINFEKL (OVA) peptide for 5h at 37°C in the presence of Brefeldin A (Sigma–Aldrich) for the last 2h before staining ...
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bioRxiv - Immunology 2023Quote: ... 18 h after cell transfer recipient mice were immunized into the right hind paw with 50 μl of a 1:1 emulsion of 1 mg/ml MOG35-55 peptide (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Immunology 2023Quote: ... injected into the left and right flank with 100 μl of an emulsion of MOG35-55 peptide (500 μg/ml) (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (made from incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with buffer H containing 0.1 M NaCl and 100 μg/mL of FLAG peptide (Sigma Aldrich, #F3290). Peak fractions were pooled and loaded onto a MonoQ 5/50GL column (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... Each group (n = 6) was immunized intraperitoneally with peptides-KLH (10 μg/mouse) emulsified in Incomplete Freund’s Adjuvant (Sigma, Cat.#F5506), and a control group (n = 6 ...
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bioRxiv - Neuroscience 2023Quote: ... Pull down of Ago proteins (Ago 1-4) was performed by incubating 500 μg of Flag-GST-T6B peptide 111 (Sigma #M8823) with 80 μl of anti-Flag M2 Magnetic beads (Sigma #M8823 ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells used for proteome-derived peptide library generation were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and SKR-2-containing protein complexes were eluted overnight in 100 µl of the lysis buffer containing 2 mg/mL of 3xFlag peptide (Sigma F4799). Eluted protein samples were digested with trypsin and analyzed by MudPIT in the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine.
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... The desalted peptide solution was analyzed by LC-MS/MS using a C18 ZipTip® (Millipore, Burlington, MA, Cat. No. ZTC18S096).
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bioRxiv - Molecular Biology 2023Quote: ... Each recombinant protein was eluted twice in 250 µl of EB containing 1 mM DTT and 250 µg/ml 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... before incubation in 60 µL 3xFLAG elution solution (146 ng/µL of 3xFLAG peptide [Sigma-Aldrich, F4799] in 0.97X equilibration buffer) at 25°C with shaking at 1,100 rpm for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were then washed three times with lysis buffer and eluted in TB buffer supplemented with 0.5mg/mL 3xFlag-peptide (SIGMA F4799-4MG). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted by two rounds of thirty minute incubations on ice with 200 ng per μL 3X FLAG Peptide (SIGMA, F4799) in TBS.
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted by two rounds of thirty minute incubations on ice with 200 ng per μL 3X FLAG Peptide (SIGMA, F4799) in Native Elution Buffer.
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bioRxiv - Biochemistry 2024Quote: ... and Cdc13 was eluted by incubating the resin with 2 mL of K buffer supplemented with 200 µg/mL of 3x FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using Amicon Ultra 0.5 mL 100 k MW cutoff centrifuge filters (Millipore) ...
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bioRxiv - Physiology 2024Quote: ... was incubated for 3 h at 4 °C with 1 μl of mouse anti-Flag M2 antibody or 3X Flag peptide (200 mg/ml; Sigma-Aldrich) on a rocking platform ...
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bioRxiv - Biophysics 2024Quote: ... NEDD4-2 HECT domain was subsequently isolated from the supernatant with anti-FLAG antibody (M2) affinity chromatography and eluted with 100 μg/mL FLAG peptide (Sigma Millipore) in 50 mM TrisHCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide clean-up was performed on mixed-phase columns (PreOmics®) according to the manufacturer’s protocol using triethylamine (TEA, Sigma-Aldrich) instead of NH4OH in the elution buffer due to subsequent TMT labelling ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were treated with 50-70 µL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10], 1% Bovine Serum Albumin (BSA, Sigma-Aldrich, A1595-50ML), 8.5 mL PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were permeabilized for 1 min with 0.1% Triton X-100 in PBS and saturated with blocking buffer (1% BSA in PBS) before incubation for one hour with anti-α-Tubulin (T5168. SIGMA; 1:2.000) in PBS containing 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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bioRxiv - Neuroscience 2020Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Monolayers were permeabilized with 0.5% Triton X-100 and blocked with a blocking buffer containing 10% goat serum (cat# G9023, Sigma-Aldrich, St. Louis, MO) and 1% bovine serum albumin (cat# A3803 ...
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bioRxiv - Neuroscience 2020Quote: ... and treated with blocking solution containing 1 % bovine serum albumin (Fisher Bioreagents, Cat.# BP1605-100) and 10 % normal goat serum (Sigma-Aldrich, Cat.# G9023) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Slots were washed with one volume of DPBS before washing the whole membrane with TBS and blocking with 5% skimmed milk powder (Sigma-Aldrich, Munich, Germany) in TBS for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times with 1X PBS prior to blocking with 3% bovine serum albumin (BSA; Sigma #A7906, Saint Louis, MO) in 1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... Organoid sections were air dried and washed three times for 10 minutes at RT followed by blocking and permeabilizing for 60 mins with a solution containing 3% bovine serum albumin (Sigma, Cat. A9418-50G) and 0.1% Triton X-100 in 1XPBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were blocked in 1:1 mixture of 1XPBS : Intercept PBS blocking buffer (LiCor) and then incubated with anti-PAP (Sigma, P1291, lot 92557) or anti-MYC (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... The wells were subsequently blocked for 1 hr in blocking solution (25 µL of 1% BSA [Sigma], 0.2% Tween-20 in 1x PBS) then incubated with 25 µL of the Click-iT EdU detection mix (prepared as per manufacturer’s instructions using Alex Fluor 455® ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664, 1:4000 anti-α-Tubulin Sigma #T5168 ...
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bioRxiv - Cell Biology 2022Quote: ... The tissue sections where fixed in -20 °C methanol for ten minutes followed by a blocking cycle (5×3 min) using phosphate buffered saline containing 1% bovine serum albumin (Sigma-Aldrich, Copenhagen, Denmark). The samples where then incubated overnight at 4 °C with the primary antibodies (dilution factor ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100) at room temperature and blocked in KTBT + 20% sheep serum + 2% Blocking Reagent (11096176001, Sigma-Aldrich, St. Louis, MO) for 4 h ...
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bioRxiv - Neuroscience 2019Quote: ... Free-floating slices were washed for 3 × 10 min periods in PBS before blocking and permeabilization for 2 h with 0.3 % Triton X-100 in PBS and 4% normal goat serum (Sigma-Aldrich, Oakville, Ontario, Canada). Slices were incubated with streptavidin conjugated with Alexa Fluor 555 or 546 (1:1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.1% Triton X-100) at room temperature and blocked in KTBT + 20% sheep serum + 2% Blocking Reagent (11096176001, Sigma-Aldrich, St. Louis, MO) for 4 h ...
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bioRxiv - Neuroscience 2021Quote: ... and rehydrated for 20 mins with 1X PBS, followed by blocking buffer containing permeabilization solution (Myers et al., 2014) with either 5% normal donkey (EMD Millipore, S30-100ml) or goat serum (Thermofisher scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and then incubated in blocking solution containing rabbit anti-DsRed antibody (1:1000; EMD Millipore, Billerica, MA) with gentle agitation at 4°C for 18-22 hrs ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... in PBS (blocking buffer) was used to block the cells for one hour and replaced with anti-FLAG antibodies (Sigma Aldrich, F-1804) diluted in blocking buffer (1:1000 ...