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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: Saturated salt solutions were prepared by adding Mg(NO3)2·6 H2O and Co(NO3)2·6 H2O (both from Sigma-Aldrich Sweden AB) to H2O (Milli-Q quality ...
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Dopamine depletion can be predicted by the aperiodic component of subthalamic local field potentialsbioRxiv - Neuroscience 2021Quote: ... 4 or 8 μl of 6-OHDA (5 μg in 1 μl saline containing 0.1% ascorbic acid; Tocris, Bristol, ENG and Sigma-Aldrich, MA, USA) was injected into the left MFB at the following coordinates ...
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bioRxiv - Bioengineering 2020Quote: ... and 800 mg L-1 humic acids (CAS No. 1415-93-6) were prepared by adding in ultrapure water (Sigma Aldrich, UK).
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bioRxiv - Cell Biology 2021Quote: ... lentiviral particles were generated by transfecting HEK293T cells with 6 μg PLKO.1 scramble control and MISP-targeted shRNA plasmids (SIGMA; TRCN0000422523, TRCN0000116527), 4 μg psPAX2 packing plasmid (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected 6 days after plating in 1 ml PBS and incubated with 60-80 ul collagenase IV [10mg/ml] (Sigma #C5138-1G) at 37 C for 10 mins ...
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bioRxiv - Biochemistry 2020Quote: ... cells were fixed using a 6% (v/v) formaldehyde: phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
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bioRxiv - Immunology 2020Quote: ... Th-1 and Th-17 cells were restimulated for 6 hr in the presence of PMA (100 ng/ml, Sigma, Cat#P8139), Ionomycin (1 μM ...
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bioRxiv - Immunology 2021Quote: ... BMDC clusters were harvested on day 8-10 in 5 mls of comlete I-10 media at room temperature and then stimulated at 1-2x106 cells per well of an untreated 6-well plate (Sigma M9062-100EA) in 2 mls of I-10 medium without GMCSF.
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bioRxiv - Genetics 2019Quote: ... 0.1%SDS and 1% Tween 20 and re-stained for the loading using the anti-glucose-6-phosphate dehydrogenase antibody (Millipore, cat #A9521). Blots were again imaged on ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were permeabilized for one hour with 0.5 % Triton X-100 and 6 % FBS in PBS and further incubated for another hour with mouse anti-FLAG M2 (1:500; Sigma-Aldrich F1803) in PBS with 0.1 % Triton X-100 and 6 % FBS (room temperature) ...
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bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
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bioRxiv - Genomics 2022Quote: The stable cell lines constantly expressing the minigenes were subjected to four time points (0, 3, 6, and 9 h) of post actinomycin D treatment (final concentration of 1 μg/mL; Sigma, no. A9415) treatment in three biological replicates ...
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bioRxiv - Genomics 2024Quote: ... neural crest cells were passaged onto fibronectin-coated 6-well plates (about 1 million cells per well) using Accutase (Sigma-Aldrich A6964) and fed with neural crest maintenance medium (1:1 ratio of DMEM-F12 and neurobasal ...
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bioRxiv - Neuroscience 2024Quote: Zebrafish larvae at 5 and 6 dpf were embedded in 1% low-melting temperature agarose diluted into MilliQ water (Sigma-Aldrich, USA). The fish were covered with artificial cerebro- spinal fluid (ACSF ...
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatants containing lentiviruses were mixed 1:1 with target cell media and supplemented with 4 mg/ml polybrene (Sigma-Aldrich cat #107689). Successfully transduced cells were selected using puromycin (Cedarlane cat #13884-50) ...
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bioRxiv - Bioengineering 2020Quote: The following primary antibodies were incubated for 1 hour at room temperature or for 12-18 hours at 4°C for the following markers: rabbit PCM-1 (1:100 dilution, Sigma-Aldrich, HPA023370-100UL), mouse PCM-1 (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were refed with fresh media containing puromycin (4 μg/ml) and doxycycline (Sigma, D9891; stock 1 mg/ml, diluted 1:1000), which was also added to the cells daily thereafter ...
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bioRxiv - Neuroscience 2019Quote: ... for 15 min at room temperature and stained overnight with the primary antibodies at 4 °C (Kinesin heavy chain: Millipore MAB1614 1:50; Poly-GR: Millipore MABN778 1:50). The following day ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated overnight at 4°C in primary antibodies (anti-lamin B1, ab16048, Abcam, 1:5000; anti-β-Actin, A1978, Sigma, 1:10,000) in 4% BSA/TBS + 0.025% sodium azide ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.3% Triton-X 100 in PBS for 1 hr before a 48-hr incubation at 4°C in 1:1,000 mouse anti-TH (Millipore; St. Louis, MO, USA) and 1:3,000 rabbit anti-cFos (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking buffer at the following dilutions and incubated on tissue sections at 4°C overnight: Calb1 (Swant, CB-38a, 1:1000) and Pvalb (Millipore-Sigma, MAB1572, 1:1000). Sections were then washed three times PBS and incubated in anti-mouse or anti-rabbit fluorescently conjugated secondary antibodies (Invitrogen Life Technologies ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... membranes were incubated overnight on gentle agitation at 4°C with primary antibodies (TH, 1:1000; GAPDH, Sigma, G8795, mouse monoclonal, 1:10,000) diluted in antibody buffer (1:1 of Tris buffered saline (TBS ...
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bioRxiv - Neuroscience 2023Quote: ... overnight and then with primary antibodies for 48 h at 4°C: rabbit-α-rab3 (1:250; provided by S.J. Sigrist, unpublished) and chicken-α-GFP (1:150, Sigma Aldrich, AB_90890). After a further round of washing ...
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bioRxiv - Physiology 2024Quote: ... pH 7.5 with 0.1% Tween20) at RT for 1 hour and subsequently incubated with primary antibodies overnight at 4°C (Actin (Millipore Sigma; A2228; 1:10000), IP3R2 (Antibody Research Corporation ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were mixed with an equal volume of 1× PBS solution containing 1 mM potassium 4-methylumbelliferyl sulfate (SIGMA ALDRICH, Castle Hill, Australia) and 2 mM phenol (SIGMA ALDRICH ...
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bioRxiv - Bioengineering 2022Quote: Acetone and Irgacure 2959 (2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1- propanone were obtained from Sigma-Aldrich (St. Louis, MO). 3-([2-(Methacryloyloxy)ethyl]- dimethylammonio)propionate (CBMA ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 3 days at 4°C with rabbit anti-AVP (1:2,000, Sigma, PC234L) or rabbit anti-Ntng1 (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... for 1 h and cultured for 4 h in the presence of 0.5μg/ml Brefeldin A (SIGMA), 0.005mM Monensin and 0.2μg/ml anti-CD107a-APC antibody ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for β3-tubulin (anti-mouse IgG, 1:500, Sigma followed by Alexa 488 anti-mouse ...
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bioRxiv - Molecular Biology 2019Quote: ... From the beginning of the incubation time medium was supplemented with the ROS inhibitor Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl)) (1mM, 176141-1G, Sigma), the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide ...
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bioRxiv - Biophysics 2020Quote: ... 4 ng/µl of the multiple-labeled goat anti-mouse IgG secondary antibodies (Sigma-Aldrich, SAB3701063-1) for the β-tubulin samples and 4 ng/µl of the multiple-labeled goat anti-rabbit IgG secondary antibody DOL 8.3 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... larval sections were counterstained with DAPI (4′,6diamidino-2-phenylindole, Sigma-Aldrich; 1:1000 μL PBS dilution) and incubated for 15 min in the dark at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated at 4°C overnight with primary antibody rabbit anti-RBM14 (1:100, Sigma-Aldrich) and then at room temperature for 1 hour with biotinylated goat anti-rabbit IgG (Vector Laboratories) ...
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bioRxiv - Biophysics 2022Quote: Cells were treated for 4 hours with media containing 1 µg/mL actinomycin D (Sigma, A5156-1VL) dissolved at 0.5 mg/mL in DMSO (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 1 h and eluted with 0.1 mg/mL 3X FLAG peptide (Sigma-Aldrich). E-buffer (20 mM Na·HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged at 1/8 ratio every 3 or 4 days with Accumax (Millipore, ref. SCR006).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were incubated overnight at 4 °C with an anti-rhodopsin mouse (Sigma-Aldrich, MAB5316, 1:300) or anti-Iba1 rabbit antibody (Fujifilm Wako Chemicals ...
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bioRxiv - Bioengineering 2021Quote: ... in the BRB80 buffer (80 mM Pipes, 1 mM EGTA, 4 mM MgCl, pH 6.8, Sigma-Aldrich) for 1 min ...
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bioRxiv - Microbiology 2020Quote: Mock or bRSV-infected (at an MOI of 1) MDBK cells were fixed in 4% PFA (Sigma) at 6 ...
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bioRxiv - Cancer Biology 2021Quote: ... and then incubated overnight at 4 ℃ with primary antibodies against human Kv1.3 (1: 1000, P4497, Sigma, USA), P-p38 MAPK (1 ...
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bioRxiv - Microbiology 2020Quote: ... 1 µl of matrix (α-Cyano-4-hydroxycinnamicacid, HCCA, CAS Number 28166-41-8, Sigma-Aldrich, Switzerland) was added ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged at 500 g at 4°C for 5 min and resuspended in 1% formaldehyde (Sigma-Aldrich) for 20 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Polymerization of 1 M monomer was performed with 4 mol% N,N’-methylene-bis-acrylamide (Sigma-Aldrich) as a crosslinker with 0.1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... iPSC-derived mesodermal cells were fed with basal medium supplemented with 4 μM IWR-1 (Sigma-Aldrich) for 48 hours ...
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bioRxiv - Plant Biology 2022Quote: ... they were supplemented once per week with 1/4 strength Hoagland No.2 media (Sigma Aldrich H2395). For tissue harvesting ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530 ...
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bioRxiv - Neuroscience 2021Quote: ... These blots were then probed overnight at 4°C 1:1000 with GAPDH antibody (EMD Millipore Corp), washed 3 times with PBST ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated at 4°C in blocking solution with either 1:200 SOM antibody (MAB354, Millipore) or 1:5000 anti-DDDDK tag (anti-FLAG Tag to label smFP ...