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Citations for Millipore Sigma :
2351 - 2400 of 4279 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 60µg of total protein per sample and 5µg recombinant Tau (rTau) (2N4RTau and 1N3R Tau) were digested with either Trypsin Protease (Pierce) or LysargiNase (EMD Millipore) at 1:50 w/w overnight at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: Bovine OVGP1 (bOVGP1) and murine OVGP1 (mOVGP1) recombinant proteins were purified using the Amicon Pro Purification System (Merck Millipore Ltd) by combining metal chelation chromatography with an Amicon concentrator membrane ...
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bioRxiv - Neuroscience 2024Quote: ... and recombinant protein isolated by GST column purification and buffer exchanged into PBS using Amicon Ultra 10K MWCO centrifugal filters (Millipore). Recombinant proteins were quantified using a BCA assay (Pierce ...
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bioRxiv - Plant Biology 2024Quote: ... the proteins were loaded onto GST-trap and the elutes containing the recombinant proteins were pooled and subjected to thrombin protease (Sigma) digestion ith 2 L buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... WT mice (male and female) were immunized intraperitoneally with 20 µg recombinant murine (rm) MPO (69) in Freund’s complete adjuvant (FCA; Sigma-Aldrich) and boosted subcutaneously with 10 µg rmMPO in Freund’s incomplete adjuvant (FIA ...
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Plant Biology 2024Quote: ... One gel was stained with coomassie brilliant blue to reveal MBP and MBP-BIM1 and the other was transferred to a PVDF membrane perform western blot analysis using primary antibody anti-HIS made in mouse (SIGMA, H1029 dilution 1:3000) and the secondary antibody anti mouse IgG used for the CoIPs.
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bioRxiv - Cancer Biology 2021Quote: ... human epidermal growth factor (EGF, 20 ng/ml; Sigma-Aldrich, E9644), human fibroblast growth factor (FGF ...
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ug/ml of Ephrin-A5/Fc (chimera human, Sigma, E0628) and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-human MLKL (Sigma-Aldrich M6697; 1:1000/1:200), rabbit anti-RIPK3 (ProSci #2283 ...
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bioRxiv - Immunology 2021Quote: ... with 0.05% human serum albumin (HSA, Sigma-Aldrich, St. Louis, MO) added to the isolation buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was diluted in 1% human AB serum (Sigma-Aldrich) in PBS at the concentrations in Table 2 and incubated overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Forty-five µl of human tubal fluid medium (HTF; Millipore Sigma) containing 4 mg/ml BSA (HTF-BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... and 48 hours later 5 IU human chorionic gonadotropin (hCG, Sigma). Then females were mated with male Hbxip+/- mice overnight ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from human dermal fibroblasts (106-05A,Sigma) transfected with Dharmacon siRNA cocktails as follows ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... 25μg/ml human insulin (Sigma-Aldrich, MO, US; Cat. No. #I9278) and 100 nM RA (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... The Milliplex Map Human TH17 Magnetic Bead Panel (Millipore, Burlington, MA) was used to measure IL-33 levels in thawed supernatants at the University of Chicago Immunology Core facility using standard protocols ...
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bioRxiv - Cell Biology 2020Quote: ... full length CRMP5 (DPYSL5, amplified from human brain cDNA library, Novagen), Ephrin receptor A1 cytoplasmic tail (aa 568-976 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... immediately transferred in 250 μl human tubular fluid medium (Millipore, Billerica), punctured with a needle and placed at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 100μg/mL Matrigel (Corning ...
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bioRxiv - Immunology 2021Quote: ... or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... Anti-Human IgG −Peroxidase antibody (Fab specific) produced in goat (Sigma) diluted 1:45000 in sample buffer was then added and samples were incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies against mouse or human laminin (1:300 rabbit, Sigma), mouse collagen IV (EMD Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... containing 1% non-autologous human plasma (Sigma-Aldrich, St Louis, USA), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Bioengineering 2020Quote: ... coated with 50 μg/mL collagen from human placenta (Sigma Aldrich) at ~10,000 cells/cm2 ...
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bioRxiv - Bioengineering 2021Quote: ... 10 ng/mL luteinizing hormone from human pituitary (LH, Sigma, L6420), 1 μM metribolone (Toronto Research Chemicals ...
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bioRxiv - Biochemistry 2020Quote: Alpha-1-acid glycoprotein (AGP) purified from human serum (Sigma #G9885) and A/Philippines/2/1982 (Phil82 ...
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bioRxiv - Biochemistry 2020Quote: PDP-23 was mixed with human pooled male serum (Sigma-Aldrich) to a final concentration of 50 µg/mL and incubated at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: In human HEK CLASH beads conjugated with anti-Flag M2 (Sigma), in FLASH Protein A agarose conjugated with IgG and anti-FBL AB were used ...
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bioRxiv - Microbiology 2020Quote: ... human serum and endothelial growth supplement were from Sigma (Taufkirchen, Germany).
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bioRxiv - Microbiology 2021Quote: ... Cells were then stained with anti-human-IgG-FITC (Sigma, F9512) or anti-mouse-IgG-FITC (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30μg/mL human plasma fibronectin (Corning ...
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bioRxiv - Microbiology 2020Quote: ... Results were normalized to ng actin using Human DNA standard (Sigma). Primers used for quantification of actin were ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were concentrated using Amicon Ultra-4 5 kDa (Millipore). Antibody concentration was determined by absorbance measurement at 280 nm using a Nanodrop and purity was determined using SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: Human gastric tissues were fixed in 4% paraformaldehyde (PFA – Sigma-Aldrich) for 2 hours and embedded in paraffin wax ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-human CD28 antibody (5μg/ml) (Millipore #MABF408, Burlingtone, MA) simultaneously for 2 hours in 5% CO2 and air at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... in AIM V (Thermo) supplemented with 5% human serum (Sigma #H3667) and incubated at 37°C/5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... in AIM V (Thermo) supplemented with 5% human serum (Sigma #H3667) and incubated at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... and 2% (v/v) of human serum (Sigma-Aldrich, cat. #H4522). BBB working medium was used for co-culture and assembloids ...
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bioRxiv - Cancer Biology 2022Quote: Secretome analysis was performed using Luminex Human 80 plex (EMD-Millipore) at Stanford Human Immune Monitoring Center ...
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bioRxiv - Biochemistry 2022Quote: GS-GN was incubated with α-amylase from human saliva (Sigma) to deglycosylate the complex ...
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bioRxiv - Cell Biology 2022Quote: ... Inserts were coated with 300 µg/mL human collagen IV (Sigma) solution in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... were pre-coated with 300 µg/mL human collagen IV (Sigma) solution in PBS ...