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Citations for Millipore Sigma :
2351 - 2400 of 2421 citations for RPL3L Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... After cell permeabilization and blocking with 1% normal goat serum (Nacalai Tesque) in dilution buffer [0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) in PBS containing 3% bovine serum albumin (Nacalai Tesque)] ...
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bioRxiv - Cell Biology 2022Quote: ... Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-αTubulin (Sigma, T6074, 1:1000 dilution), anti-acetylated Tubulin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in 50 μl of blocking buffer were added for 1 h followed by 50 μl ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5.000 dilution) in blocking buffer for 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... iSMCs were incubated for 30 mins in blocking solution containing 2% BSA (AppliChem, 9048-46-8) and 0.5% Triton™ X-100 (Sigma-Aldrich, 9002-93-1). The blocking solution was then discarded and iSMCs were incubated for 2 hrs with primary antibodies diluted in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking for 1 hour in blocking buffer (for Fig. 1D: 5% Protifar; for Suppl. Fig. 2B: 0.2% v/v Fish skin gelatin (Sigma–Aldrich, St. Louis, MO, USA)+ 0.1% v/v Tween–20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated in blocking buffer for 1 hr at room temperature followed by incubation with rabbit anti-RBM10 (Sigma-Aldrich HPA034972, 1:2000), rabbit anti-HDAC1 (GeneTex GTX100513 ...
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bioRxiv - Neuroscience 2023Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Genetics 2024Quote: ... by transferring them in a small drop of blocking solution to the surface of a 0.01% poly-L-lysine (Sigma Aldrich, Cat# 25988-63-0) coated slide ...
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bioRxiv - Cell Biology 2024Quote: ... Permeabilization buffer was removed and washed 3×2 minutes before blocking for 1 hour at room temperature in a 1% Bovine Serum Albumin (BSA) (Millipore Sigma, cat: 2910-OP) in DPBS solution ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were subsequently washed five times with ice-cold lysis buffer freshly supplemented with 20 mM NEM and subsequently incubated with Flag peptide (2 mg/mL, Sigma-Aldrich, F-3290) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The immunoprecipitates on protein A agarose or sepharose were either boiled directly in sample buffer or eluted with FLAG peptide (Sigma, 200 μg/mL) or HA peptide (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The selected mouse and rabbit immunoglobulins were affinity purified on protein G SepFast resins (BioToolmics, UK) or on peptide used for immunization and conjugated to a cyanogen bromide-activated-Sepharose® 4B (Sigma-Aldrich, USA), respectively ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... BG-oligonucleotide-labeled SNAP proteins bound to resin were again washed with wash buffer and eluted using 0.2 mg/mL FLAG peptide (Sigma-Aldrich, St. Louis, MO) overnight at 4°C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads were washed 3 times with lysis buffer and TFEB or TFE3 eluted using 200 ng/μL 3X-flag-peptide (Sigma-Aldrich, F4799-4MG).
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bioRxiv - Biochemistry 2020Quote: ... isolated from the BBPHs were added to the reaction mix before adding the enzyme to assess the inhibition potential of the peptides and the results have been compared with a standard ACE inhibitor Lisinopril (L2777, Sigma-Aldrich, MO, USA), used as positive control (50 µL of 2 µg/mL).
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bioRxiv - Immunology 2020Quote: ... and in vitro cultured for 18-24 hours in media supplemented with or without peptide at 0.5 µM (or, as positive control, with 1 µg/ml Staphylococcal enterotoxin B (SEB, Sigma Aldrich, St. Louis, USA)) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bead-particle complexes were washed once with 200 µl washing buffer (20 mM Tris-HCl pH 7.5 and 150 mM NaCl) and subsequently eluted with FLAG peptide (30 min at 37 °C; 200 μg/ml in washing buffer, Sigma Cat. No. F3290) and lysed by addition of Amphipol A8–35 (Anatrace ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Cell Biology 2021Quote: The peptide/ZO1 interaction was detected in situ using the Duolink secondary antibodies and detection kit (Sigma, St Louis, MO, Cat: 92002, 92004) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were washed two times with 1 mL Pierce Lysis Buffer using a magnetic rack and eluted in 100 μl Pierce lysis buffer containing 3X FLAG® Peptide (Sigma, Cat. # F4799) at 20 μM ...
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bioRxiv - Cell Biology 2023Quote: ... the Anti-Flag M2 Affinity Gel was incubated with 2x vol of 100 µg/ml Flag-Peptide (in TBS, Sigma-Aldrich, cat.no. F3290) at 4 °C and 350 rpm agitation for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were eluted from Flag magnetic beads through adding 5 package of beads gel volume of 150 ng/μl 3 x Flag peptide (Sigma-Aldrich, cat # 4799) in 1 × TBS buffer and incubating in a rotator for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with Li-COR Odyssey Blocking Buffer and then incubated with primary antibodies: anti-FLAG (Sigma F1804, 1:1000 for proSAAS and 7B2), anti-TDP-43 (Proteintech 10782-2-AP ...
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bioRxiv - Microbiology 2020Quote: ... incubated with the anti-PnpA quail antibody (1:10000 dilution) in blocking buffer followed by incubation with an anti-chicken alkaline phosphatase conjugated secondary antibody (Sigma, Ref A9171, 1:10000 dilution) and NBT/BCIP development.
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bioRxiv - Cell Biology 2021Quote: ... the cells were blocked with the blocking buffer (3% bovine serum albumin [BSA, Sigma-Aldrich A3059] and 0.1% saponin [Sigma-Aldrich S4521] dissolved in DPBS) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Unspecific antibody binding was prevented by incubating the blots in blocking solution containing 5% non-fat milk or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in 1x Tris-buffered saline with 0.1 % Tween 20 (TBST ...
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bioRxiv - Genetics 2021Quote: ... followed by blocking with milk or bovine serum albumin (BSA) and antibody labelling with specific antibodies to FLAG (Sigma F1804; 1:5000 in 3 % milk) or RPS-6 (Abcam ab70227 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by blocking the non-specific binding sites with PBS supplemented with 1%v/v bovine serum albumin (BSA) (Sigma Aldrich, St. Luis, MO, USA). The cells were then incubated with primary antibody rabbit anti-YAP1 (1 ...
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bioRxiv - Plant Biology 2020Quote: ... the ORF of CLEL6 lacking the predicted signal peptide was amplified by PCR from a previous construct and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). Correct orientation and translational fusion with the C–terminal His-tag was verified by sequencing ...
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bioRxiv - Immunology 2021Quote: ... 1 mM penicillin-streptomycin and 55 μM β-mercaptoethanol) containing 10 nM either OVA257-264 (H2N-SIINFEKL-OH) or YopE69-77 peptide and brefeldin A (BFA, Sigma, 5 μg/ml) for 4.5 h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... The beads were washed tree times with TBS solution and FLAG-HDA6 was eluted with 200 ng/µl of Flag-Peptide (Sigma-Aldrich, Cat No F3290) by incubation the resin with synthetic peptide rotating for 30 min at 4 °C.
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bioRxiv - Plant Biology 2020Quote: ... ORFs of PSK1 and RGF1 lacking the predicted signal peptides were amplified by PCR from cDNA and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). C-terminal His tags were added by including 6 His codons in the reverse PCR primers ...
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bioRxiv - Microbiology 2019Quote: ... and added to wells in a sterile 96-well microtiter plate containing two-fold dilutions of each peptide with 0.01% v/v acetic acid (Sigma Aldrich, St Louis, MO, USA) and 0.2% w/v bovine serum albumin (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were subsequently washed another three times with 1 ml FLAG wash buffer II and eluted in 100 μl FLAG elution buffer (FLAG wash buffer II with 0.5 mg/ml Flag peptides (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066, RRID: AB_476693, immunogen: synthetic peptide corresponding to the C-terminal actin fragment SGPSIVHRKCF, Sigma-Aldrich, St. Louis, MO, USA), and rabbit polyclonal anti-AA-NAT 1:15,000 diluted in PBST (AB3314 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated with increasing concentrations of unlabelled peptides (D5, TPD5, mPD5) using black half-area Corning non-binding surface 96 well plates (Sigma-Aldrich, Ref. no. 3686). The plates were incubated 20 min on ice and the fluorescence polarization was measured on an Omega POLARstar plate (BMG LABTECH ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were immunized on day 0 by subcutaneous injections in the flank region with a total 200 μl of antigen PLP139-151 peptide (100 μg/mouse) emulsified in Complete Freund’s Adjuvant CFA (Sigma Chemicals, St. Louis, MO, USA) supplemented with 4 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (400 μg ...
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bioRxiv - Neuroscience 2021Quote: ... and immunoblotted overnight at 4°C with shaking in Intercept Blocking Buffer with the following antibodies: α GluA2 (1 µg/mL; Millipore, MAB397 or Alomone, AGC-005); α GluA1 (1 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... after which the slices were washed with the blocking solution 3 times and stained with the nuclear marker DAPI (Sigma–Aldrich, 1 mg/ml 1:1000) for 15 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1:500 was used to detect myc tagged ATR and incubated at room temperature for 1 h in blocking buffer (1 % BSA [Sigma], 0.2 % Tween-20 in 1x PBS) in a wet chamber ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by 1-hour incubation at RT with the diluted secondary antibody after which the slices were washed with the blocking solution 3 times and stained with the nuclear marker DAPI (Sigma–Aldrich, 1 mg/ml 1:1000) for 15 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies used in this study were: anti-acetylated α-tubulin (Antibody registry ID: AB_477585, clone 6-11B-1, mouse IgG2b, Sigma, diluted 1:250 in blocking solution), anti-SV2 (AB_2315387 ...