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Citations for Millipore Sigma :
2351 - 2400 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were seeded 24 hours prior to transduction at a density of 50,000 cells per well in 6-well plates and transduced with lentiviral vectors in the presence of 5 µg/ml polybrene (Millipore). When cells were transduced with a Pro-Code library ...
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bioRxiv - Immunology 2021Quote: Blood was extracted from hBLT mice at 3 and 5-6 weeks post-infection and lysed with Red Blood Cell Lysis Buffer (SIGMA). T cells were activated for 1.5 h with 5μl/ml of anti-CD28 and anti-CD49d in the presence or absence of 6.4μg/ml of a Gag pool of peptides in the presence of 0.5μg/ml Brefeldin A ...
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bioRxiv - Cell Biology 2019Quote: Cold APC purification buffer (100 mM NaPi, Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670 ...
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bioRxiv - Microbiology 2021Quote: ... Exponentially growing cells pre-cultured in the presence or absence of Mn were harvested and resuspended at a density between 1E+07 and 2.5E+07 cells/mL in their corresponding growth medium supplemented with erythromycin (5μg/mL) and 10μM 5(6)-carboxyfluorescein (Sigma-Aldrich 21877), with or without Mn (20μM) ...
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bioRxiv - Microbiology 2022Quote: MDCK-SIAT1 cells were infected with the PR8 NP:WT/F346S viruses at an MOI of 5 TCID50/cell in a 6 well plate in the presence of 100µg/mL of cycloheximide (Sigma-Aldrich). Infected cells were harvested at 2 and 6hrs post-infection ...
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bioRxiv - Plant Biology 2019Quote: ... ten first or second leaves from independent plants were harvested at the indicated time points and transferred to a 15 ml tube containing 6 ml water purified using a Milli-Q integral 5 water purification system (Millipore). After a 6 h-incubation at room temperature on a horizontal shaker ...
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bioRxiv - Molecular Biology 2019Quote: ... U2OS cells were seeded in a 6-well plate and infected with lentivirus supernatant in the presence of 5 μg/mL polybrene (Sigma). Medium was replaced by fresh media with 10 μg/mL Blasticidin S at 24 hours post-infection ...
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bioRxiv - Developmental Biology 2020Quote: ... The pregnant mice were serially scanned at E6.5 either after intra-peritoneal administration of 1µg/g BW of hyaluronan synthesis inhibitor 6-diazo-5-oxo-1-norleucine (DON, Sigma-Aldrich) at E3.5-E5.5 ...
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bioRxiv - Cell Biology 2019Quote: ... The resin was resuspended in 6 ml ice-cold G-buffer containing 5 µg/ml TLCK-treated chymotrypsin (Sigma, #C3142) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cells were then treated with 6-thio-dG (3-5 μM) in the presence of 500 ng/ml doxycycline (Sigma), washed three times with warm PBS and allowed to recover in regular growth medium containing 500 ng/ml doxycycline ...
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bioRxiv - Immunology 2022Quote: ... synthesized as previously described),6 a 5 mg/mL stock solution of PBI1 dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich) was prepared ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Bioengineering 2024Quote: ... the 3D collagen matrices were stained with 50 µM 5-(6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE; Sigma-Aldrich, Darmstadt, Germany) overnight at room temperature and subsequently rinsed with phosphate-buffered saline (PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... HEK-293T cells were cultured in 25 cm2 vented flasks at 37°C in a humidified 5% CO2 incubator in 6 mL of DMEM supplemented with 10% FBS and 0.5 mL of penicillin/streptomycin (Sigma-Aldrich, Canada ...
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bioRxiv - Immunology 2023Quote: ... Interphase cells were collected and incubated at 37°C in 5% CO2 for 6 hours with 20ng/ml Phorbol 12-Myristate 13-Acetate (PMA, Sigma), 1µg/ml Ionomycon (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... 3D collagen matrix systems were stained with 50 µM of 5-(and-6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE, Sigma-Aldrich) at room temperature for 60 minutes and visualized using a confocal laser scanning microscope (cLSM ...
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bioRxiv - Microbiology 2023Quote: ... a dilution series of 107 to 103 cells/ml were plated in 5 μl spots on M9 minimal media plates with 10 mM glucose or 10 mM glucose-6-phosphate (Sigma) and antibiotics ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and then seeded in 6-well plates (5×105 cells/well) that had been pre-coated the day before with type I collagen (Sigma) dissolved in 0.02 N acetic acid ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 30 minutes on ice with 6 nm (Jackson#711-195-152) or 5 nm (Sigma#G7277) diameter immunogold coupled secondary antibodies in DMEM-FBS-HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... 400,000 cells were plated in 6-well poly (2-hydroxyethyl methacrylate) (poly-HEMA, Sigma-Aldrich, St. Louis, MO, USA)-coated plates for the indicated amount of time in the figure legends ...
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bioRxiv - Immunology 2019Quote: ... spleens from C57BL/6 mice were cut to smaller pieces and digested with Collagenase D (2 mg/ml, Sigma) For 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... mice were fasted for 6 hours prior to IP injection of 2 g/kg 20% D-glucose (Sigma-Aldrich) in water with a 26G needle ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were then spun down (2 min with 1000 rpm) and resuspended in 6 ml serum-free medium (Sigma Cat# ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... at an age of 6-10 weeks by a single intra peritoneal injection of 2 mg Tamoxifen (Sigma; T5648) in corn oil (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... homogenised in 6 mL of 2:1 (v/v) chloroform:methanol containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma-Aldrich) as antioxidant ...
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bioRxiv - Cell Biology 2024Quote: ... 561 and 640 nm laser lines were used for excitation of DAPI (4rn,6-Diamidino-2-phenylindole, Sigma-Aldrich), Alexa Fluor 555 and Alexa Fluor 647 respectively ...
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bioRxiv - Neuroscience 2024Quote: Adult female flies aged 2–6 days post eclosion were head-fixed to a custom mount using eicosane (Sigma). Cuticle ...
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bioRxiv - Biochemistry 2019Quote: ... Knock-down of glutamate dehydrogenase 2 (GDH2, encoded by the GLUD2 gene) using siRNAs (#NM_012084: 5’CUAACCUCUUCACGUGUAA’3 and 5’UUACACGUGAAGAGGUUAG‘3, Sigma-Aldrich, Taufkirchen, Germany) or transfection of GLUD2 plasmid/empty vector controls was done using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 60 µl elution buffer (2 M urea, 50 mM Tris-HCl pH 7.5 and 5 μg/mL Trypsin (SIGMA; T6567-5×20UG)) (twice as the bead volume ...
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bioRxiv - Microbiology 2020Quote: ... 49 with 1.5% weight vol−1 agar supplemented with 2 mg mL−1 5-Fluoroorotic acid (USBiological, USA) and 5 µg mL−1 uracil (Sigma–Aldrich USA) in order to select for plasmid excision ...
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bioRxiv - Cell Biology 2020Quote: ... Unreacted dye was quenched with the addition of 5 mM 2-Mercaptoethanol (Sigma-Aldrich). Free dye was removed with 3 sequential 2 ml Zeba desalting columns (ThermoScientific ...
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bioRxiv - Cell Biology 2020Quote: ... or vehicle (PEG400 / Ethanol / Kolliphor® EL / water, 10:5:2:83, Sigma-Aldrich) in their drinking water ...
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bioRxiv - Cell Biology 2020Quote: ... anti-alpha-tubulin (Sigma, Cat no. T5618, Clone B-5-1-2; 1:200), anti-Muc1 (Cell Signaling Technologies ...
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bioRxiv - Immunology 2021Quote: ... or a monoclonal mouse anti-tubulin antibody (Sigma-Aldrich, Clone B-5-1-2). Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2020Quote: ... The cell aggregates were transferred to 5% Poly(2-hydroxyethyl methacrylate) (Sigma-Aldrich, P3932) coated tissue culture plates at day 7 and cultured in RPMI with B27 supplement until the end of differentiation ...
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bioRxiv - Molecular Biology 2021Quote: ... for 3.5 minutes at 5 μL/min with 2% ACN (Sigma Aldrich, Cat# 1207802), 0.1% formic acid (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were first treated with 10 mM 5-bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich) pulses at designated stage and then fixed with fresh 4 % paraformaldehyde (PFA ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... mouse ascites MAb B-5-1-2 α-tubulin 1:200 (Sigma-Aldrich #T5168); rat TgEB1 1:100 (Chen and Gubbels ...
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bioRxiv - Neuroscience 2019Quote: ... 2’-O-dibutyryladenosine 3’,5’-cyclic monophosphate sodium salt (db-cAMP; 1 mM; Sigma). Media was replaced every 2-3 days for a minimum of 15 days ...
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bioRxiv - Cell Biology 2019Quote: ... 3-isobutyl-1-methylxanthine (I5879) and 2’,5’-dideoxyadenosine (D7408) were purchased from Sigma. Carvedilol (15418 ...
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bioRxiv - Cancer Biology 2020Quote: SNU387 cells were treated with 1.25 uM of 5-aza-2’-deoxycytidine (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and 5-tetradecyloxy-2-furoic acid (TOFA; combination SCD-1Δ9 /ACC1 inhibitor, Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Clone B-5-1-2) (1:1000, Sigma, Cat# T5168), and mouse anti-PyCSP (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-α-tubulin 1:10,000 (monoclonal B-5-1-2, Sigma-Aldrich). To image the blot the secondary antibodies goat anti-rabbit IgG IRDye 800CW (LI-COR ...
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bioRxiv - Neuroscience 2021Quote: Mice received three intraperitoneal injections (100mg/kg) of 5’-bromo-2 deoxyuridine (BrdU, Sigma) at 4 h intervals and were sacrificed after 28 days ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 5 min with 4,6-diamidino-2-phenylindole (DAPI; Sigma, D9542), washed again three times in PBS and then mounted in Vectashield (Vector Labs) ...
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bioRxiv - Microbiology 2022Quote: ... 5-dithio-bis (2-nitrobenzoic acid) or DTNB (Sigma-Aldrich, St Louis, MO, USA), 0.1 M sodium phosphate buffer (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: Split-Cre mice received drinking water containing 5-bromo-2’-deoxyuridine (BrdU) (Sigma-Aldrich) (1 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... and rinsed with PBS containing 2% BSA and 5 mM EDTA (both Sigma-Aldrich). After centrifugation for 5 min ...