Labshake search
Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... proteins were transferred to PVDF (Millipore, IPVH00010) or Nitrocellulose (Sigma_Aldrich ...
-
bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
-
bioRxiv - Neuroscience 2019Quote: ... Total protein staining solution (REVERT Total Protein Stain kit, LI-COR Inc., # 926-11010; Pyronin Y (Sigma-Aldrich # P9172)
-
bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
-
bioRxiv - Cancer Biology 2021Quote: Protein extracts were separated from each cell line with RIPA protein lysis buffer (R0278, Sigma, St Louis, MO, USA) containing 1X protease cocktail (P8340 ...
-
bioRxiv - Cell Biology 2021Quote: ... The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 μg of protein from the different S1 fractions was first treated with PureProteome Protein G Magnetics Beads (Millipore) for contaminant IgG depletion and then directly applied to dry nitrocellulose ...
-
bioRxiv - Biochemistry 2022Quote: ... protein induction was performed on a small scale (2mL) and cells were disrupted using BugBuster protein extraction reagent (Novagen).
-
bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
-
bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
-
bioRxiv - Developmental Biology 2019Quote: ... The HDAC6 protein was immunoprecipitated from total protein lysates (0.25 mg) of cells treated with different compounds using protein A agarose beads (Sigma). The HDAC6 protein bound beads were used as enzyme source and was incubated with the HDAC assay buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Cell Biology 2021Quote: ... and protein diluted to 1mg/ml 1ml of protein lysate was precleared with 20μl of protein G sepharose bead slurry (Sigma) for 1 hour to remove native immunoglobulins ...
-
bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
-
bioRxiv - Genomics 2021Quote: ... were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V, Sigma), by 4 hrs incubation time at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein was estimated using the bicinchoninic acid protein estimation method (BCA, B9643, Sigma-Aldrich). We separated a protein sample (80µg ...
-
bioRxiv - Paleontology 2023Quote: Information for the protein standards purchased for the ToF-SIMS analyses are as follows: type-1 collagen protein (Sigma, CAS ...
-
bioRxiv - Cell Biology 2024Quote: ... Yeast protein extracts were separated on protein gels containing 10% polyacrylamide and transferred to an Immobilon-P membrane (Millipore). The membranes were probed with anti-Myc antibodies (9E10 ...
-
bioRxiv - Cell Biology 2020Quote: A2780 human ovarian cancer cells were maintained in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% (v/v ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500 ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then suspended in veronal buffer and human serum (Sigma-Aldrich, H4522) (or heat-inactivated for 30min at 56°C) ...
-
bioRxiv - Molecular Biology 2021Quote: The gene of human GAPDH was cloned into pET-28a(+) expression vector (Novagen) with an N-terminal His-tag followed by the Tobacco Etch Virus (TEV ...
-
bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
-
bioRxiv - Cell Biology 2022Quote: ... dried and incubated with 10 μg/mL of IgG from human serum (Sigma) in PBS for 1 hour at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232).
-
bioRxiv - Molecular Biology 2021Quote: ... bleomycin and human activating FAS (anti-FAS) antibody were purchased from Millipore Sigma. PGE2 ...
-
bioRxiv - Immunology 2022Quote: ... media was replenished with RPMI containing 5% type AB human serum (Sigma-Aldrich). For single-round experiments with VSV-G-pseudotyped viruses ...
-
bioRxiv - Systems Biology 2019Quote: ... The human plasma reference standard NIST SRM 1950 was obtained from Sigma Aldrich. Human cancer cells (HeLa S3 ...
-
bioRxiv - Cell Biology 2020Quote: ... in acetic acid (0.02 N) or human plasma fibronectin (5 µg/mL; Millipore) in PBS overnight at 4°C and rinsed with imaging medium to remove the coating solution ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were blocked with human IgG (1.25 mg/mL, 15 min; Sigma Aldrich). Surface staining of unfixed/unpermeabilized cells employed the following antibodies ...
-
bioRxiv - Cell Biology 2019Quote: ... PINK1 knockdown was performed using MISSION® esiRNA human PINK1 (EHU057101, Sigma Aldrich). Mff knockdown (SI Fig ...
-
Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Sections were incubated with rabbit anti-human-IgG (1:100, Sigma, SAB3701291, IgG) and with rabbit anti-human IgM (1:250 ...
-
bioRxiv - Immunology 2019Quote: ... anti-human C5aR or nonpeptide C5aR antagonist W-54011 (5 μM, Merck Millipore)(25 ...
-
bioRxiv - Cell Biology 2019Quote: ... Staining for murine and human MSCs was performed using Oil Red O (Sigma) stain for adipogenesis ...
-
bioRxiv - Cell Biology 2020Quote: The human ovarian cancer cell lines COV318 (purchased from ECACC, via Sigma-Aldrich) and SKOV3 (courtesy of Dr Florence Delie ...
-
bioRxiv - Cancer Biology 2019Quote: ... human breast cancer were detached using Trypsin (when grown as monolayer, Sigma-Aldrich) or pelleted and dissociated using StemPro Accutase (when grown as spheres ...
-
bioRxiv - Immunology 2021Quote: ... Total IgA purified from human serum was used as a standard (Sigma-Aldrich). To generate the IgA standard curve anti-human IgA capture antibodies ...
-
bioRxiv - Immunology 2021Quote: ... and then overlaid with complete DMEM containing 10 μg/mL human IgG (Sigma). 48 hours post infection ...
-
bioRxiv - Microbiology 2021Quote: ... Primary human monocytes were treated with 50 ng/ml M-CSF (Sigma-Aldrich) in complete RPMI for a week before infection ...
-
bioRxiv - Cancer Biology 2021Quote: MISSION shRNA targeting mouse or human PSME4 or RFP were obtained from Sigma (TRCN0000176569 ...
-
bioRxiv - Bioengineering 2021Quote: ... and human β2m were purchased from IDT and cloned into pET3a vectors (Novagen). H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB) ...
-
bioRxiv - Bioengineering 2021Quote: Human serum albumin (> 97% lyophilized) was purchased from Sigma Aldrich (Oakville, ON, Canada). Glutaraldehyde (25% aq ...
-
bioRxiv - Bioengineering 2021Quote: ... Serum pramlintide concentrations were quantified using a human amylin ELISA kit (Millipore Sigma). Serum lispro concentrations were quantified using Northern Lights Mercodia Lispro NL-ELISA ...
-
bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL Follicle Stimulating Hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL LH ...
-
bioRxiv - Biochemistry 2021Quote: FFPE human tonsils were deparaffinized in xylene substitute (Sigma-Aldrich, St. Louis, MO), rehydrated in a graded ethanol series and stained for 10 minutes with Atto 465-carboxcylic acid (8 uM ...
-
bioRxiv - Biophysics 2021Quote: ... lyophilized rabbit muscle aldolase and lyophilized human hemoglobin were purchased from Sigma Aldrich. The catalase was diluted to a final concentration of 2.3 mg ml-1 in the buffer of PBS (pH 6.5) ...
-
bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232). The following day ...
-
bioRxiv - Cancer Biology 2021Quote: siRNAs targeting human SUV39H1/H2 and negative control siRNAs72 were from Sigma-Aldrich. Transfections were performed using Lipofectamine RNAiMax (Thermo Fisher ...
-
bioRxiv - Biophysics 2021Quote: ... pre-coated with 30 μg/ml of fibronectin from human plasma (Sigma-Aldrich) and nanodiamonds (100 nm ...
-
bioRxiv - Neuroscience 2020Quote: ... Plates were incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...