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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for Recombinant Human IL21 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... supplemented with cytokine cocktails aimed at proliferation (50 ng/ml murine recombinant thrombopoietin (TPO, Sigma-Aldrich SRP3236-10UG) and 100ng/ml Stem Cell Factor (SCF) ...
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bioRxiv - Immunology 2019Quote: ... NMRI mice were injected subcutaneously at two different locations with 100µg of the recombinant Thio-EmACT resuspended in 100µl Freund Incomplete adjuvant (Sigma). The double injections were repeated four weeks later to boost the mice anti-EmACT response ...
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bioRxiv - Biochemistry 2022Quote: ... and ∼1 μg recombinant GST or GST-SMN in freshly made 25 mM bis tris propane (Sigma B6755) pH 6.8 buffer with 1 M NaCl and 0.05% NP-40 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells that were cultured in 0.5 % FBS were stimulated with hEGF (recombinant EGF) (Sigma Alrdich, Catalog No: E9644) in concentrations (10 and 30 ng/ml ...
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bioRxiv - Microbiology 2022Quote: ... Assay was conducted according to the manufacturer’s instructions and recombinant L-Lactic Dehydrogenase from porcine heart (Sigma-Aldrich) was used to generate a standard curve ...
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bioRxiv - Cell Biology 2023Quote: ... either coated with BSA or recombinant Laminin332 (BioLamina LN332-0502, diluted in Dulbecco’s Phosphate Buffered Saline, Sigma, D8662) and left to adhere overnight ...
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Biochemistry 2023Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Supplemented IVM media consisted of MediCult IVM media (Origio) supplemented with 75 mIU/mL of recombinant FSH (Millipore), 100 mIU/mL recombinant hCG (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... The recombinant APC/C complex was eluted with the wash buffer supplemented with 2.5 mM desthiobiotin (Sigma-Aldrich). Before loading the elution fractions onto a 6 mL Resource Q anion-exchange column (Cytiva) ...
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bioRxiv - Microbiology 2024Quote: ... with 50 μg of either recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ku80 (CST, #2180, 1:400), anti-human nuclear antigen (Novus NBP2-34342, 1:200) and anti-human-specific Albumin (Sigma, A3293, 1:200). Species-specific secondary antibodies conjugated with Alexa Fluor 555 ...
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bioRxiv - Bioengineering 2020Quote: ... of 0.3 with Edit-R lentiviral mKate2-tagged, constitutively expressed Cas9 nuclease under the hEF1α promoter (Dharmacon, CAS11229) in transduction medium containing 5 μg/mL polybrene (EMD Millipore, TR-1003-G). Complete growth medium was added to the transduced cells at a 3:1 dilution after 5 hours ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Microbiology 2020Quote: ... protein complexes were precipitated using Protein A agarose beads (Sigma, St. Louis, MO, USA). The precipitated material was analyzed by western blot ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: The protein content was analysed with the Bicinchonic Acid Protein Assay Kit (Sigma-Aldrich) according to the manufacturer’s protocol with minor adjustments ...
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bioRxiv - Cell Biology 2021Quote: The Protein A slurry was prepared by washing 1.5 g Protein A Sepharose (Sigma) 4 times in 1X Tris Buffered Saline (1X TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Bradford protein assay was used to quantify total protein levels (Bradford, Sigma-Aldrich, USA) and to normalize BDNF data.
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein precipitation was carried out using ProteoExtract Protein Precipitation Kit (EMD Millipore, 539180-1KIT) according to the manufacturer’s protocol and submitted for MS analysis to the Taplin Biological Mass Spectrometry Facility.
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Bound proteins were captured with Protein G agarose for 4 h (Sigma-Aldrich, #17061801), spun at low speed ...
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bioRxiv - Cell Biology 2023Quote: ... and total (i.e. nonphosphorylated) protein levels were normalized to tubulin protein levels (Sigma-Aldrich, #T6557 ...
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bioRxiv - Molecular Biology 2024Quote: Total carbonylated proteins were quantified using OxyBlot Protein Oxidation Detection Kit (Millipore, Billerica, MA) following manufacturer’s instructions using 15 μg of total protein detected with rabbit anti-DNP antibodies (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... and visualized using InstantBlue Coomassie Protein Stain or ReadyBlue Protein Gel Stain (Sigma Aldrich).
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... Urocortin (rat) protein (Millipore Sigma, ~2μl of 40μM in 1x PBS + 0.1% acetic acid ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Biochemistry 2020Quote: ... Protein A/G beads (Millipore) were then added for overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein G affinity beads (Sigma) were added to the lysate (for anti-D-cys immunoprecipitation only ...
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bioRxiv - Microbiology 2021Quote: ... 2x protein inhibitor complex (Sigma) in PBS was added and protein levels were normalized by Bradford assay ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0 Protein Detection System (Millipore). Membranes were imaged using a chemiluminescent SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cancer Biology 2021Quote: Protein A Dyna-beads (Millipore) were used for immunoprecipitation ...
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bioRxiv - Physiology 2022Quote: ... Protein G Agarose beads (Millipore) and AMPK-IP buffer (50 mmol l-1 NaCl ...
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bioRxiv - Biophysics 2022Quote: ... 1x Protein Inhibitor Cocktail (Sigma), 1 mM PMSF (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: BCA protein assay (Sigma-Aldrich) was performed to measure protein concentrations ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 mM Cas9 protein (Sigma), and 300 mM KCl ...
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bioRxiv - Plant Biology 2019Quote: ... Myelin basic protein (Sigma-Aldrich) was used as an artificial substrate ...