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Citations for Millipore Sigma :
2301 - 2350 of 5966 citations for QuantiChrom Iron Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 0.001% SDS to the volume of 90 µl per measurement and supplemented with 10 µl of 0.5mM proteasome substrates in the assay buffer: Substrate III (Suc-LLVY-AMC, chymotrypsin-like activity, Millipore), Substrate IV (Z-ARR-AMC ...
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bioRxiv - Physiology 2022Quote: Cell viability was measured by the colorimetric assay showing reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) as previously described (Denizot and Lang ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich) as described [29].
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bioRxiv - Microbiology 2021Quote: The following small molecule inhibitors and antibodies were used for TEER and EGL inhibition assays: ATN-161 (Sigma, SML2079), SB 431542 hydrate (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and trypsin concentration was quantified using an enzymatic assay with Nα-Benzoyl-L-arginine ethyl ester (BAEE, Sigma-Aldrich) exactly according to Rocklin et al (Rocklin et al ...
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bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Growth assays used 200μL culture volumes in 96- well plates (Greneir) sealed using Breathe-Easy® membranes (Sigma Aldrich) and measured attenuance (D ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was determined by MTT assay and measured at 570 nm according to the manufacturer’s instructions (EMD Millipore). All experiments were performed in triplicate ...
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bioRxiv - Neuroscience 2021Quote: Proximity ligation assay (PLA) was performed in primary hippocampal neurons and N2a cells according to the manufacturer’s instructions (Duolink, Sigma). Until the PLA probe incubation step ...
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bioRxiv - Neuroscience 2022Quote: One cerebral hemisphere (without the cerebellum or olfactory bulb) was homogenized in radioimmunoprecipitation (RIPA) assay buffer (Sigma-Aldrich; R0278) containing Pierce protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... PLA assay was performed using custom labelled antibodies and the Duolink In Situ Detection Reagents Red (Sigma-Aldrich, DUO92008) as described by the manufacturer but with slight modifications ...
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bioRxiv - Immunology 2019Quote: ... IL-17 and CCL17 were determined with ELISA assays according to the manufacturer’s protocols (Sigma, St. Louis, MO, USA).
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bioRxiv - Immunology 2019Quote: ... Cell lines were confirmed as mycoplasma negative before their use in experiments using a PCR-based assay (Sigma Aldrich).
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bioRxiv - Systems Biology 2020Quote: ... Metabolic hormones were measured using MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay (HMHEMAG-34K, Millipore). Cardiovascular risk markers in plasma were measured using MILLIPLEX MAP Human Cardiovascular Disease (CVD ...
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bioRxiv - Plant Biology 2019Quote: ... From the working solution an assay buffer was made adding 50U of Glucose-6-Phosphate Dehydrogenase (G6PDH; Sigma-Aldrich), and 295µL or 280µL of the working solution was added to a 96 well plate (Costar ...
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bioRxiv - Pathology 2021Quote: Proximity ligation assay (PLA) was performed according to the manufacturer’s instructions (Duolink In Situ detection reagent-Red, Sigma Aldrich). Briefly ...
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bioRxiv - Physiology 2020Quote: ... the protein content of each sample was quantified using the Bradford assay (B6916, Sigma Aldrich, Saint Louis, MI, USA) with protein standards made from bovine serum albumin (ALB001.25 ...
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bioRxiv - Physiology 2020Quote: ... and 120-minutes thereafter using a colorimetric assay (PGO enzyme preparation and dianisidine dihydrochloride, Sigma-Aldrich, St. Louis, MO). Area under the curve measurements were calculated using the trapezoidal method [32].
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bioRxiv - Molecular Biology 2019Quote: ... For gel assay reaction mixtures were loaded onto 12% SDS PAGE and then transferred onto PVDF membrane (Merck Millipore) and immunoblotted with antibodies as mentioned ...
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bioRxiv - Physiology 2021Quote: ... complete culture medium was exchanged for pre-warmed Seahorse Assay medium supplemented with 10mM glucose (Sigma-Aldrich, Poole, UK), 1mM sodium pyruvate (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Total protein concentration was determined using the Bradford assay with bicinchoninic acid (BCA) reagent (Millipore Sigma, St. Louis, MO). Total protein (40 μg ...
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bioRxiv - Genomics 2021Quote: ... and solubilized in IB buffer MB and HS fractions were estimated by Bradford assay or Direct Detect spectrophotometer (Millipore). Protein samples were solubilized in Laemmli buffer with 50 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... was prepared in USP30 activity assay buffer (50 mM Tris base, pH 7.5, 100 mM NaCl, 0.1 mg/ml BSA (Sigma, A7030), 0.05% Tween 20 ...
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bioRxiv - Pathology 2021Quote: ... of fresh frozen villous placental tissue samples were homogenized into the assay lysis buffer containing 0.1% Triton-X 100 (Sigma) and further processed per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured for 72 h and then cell death was measured using the tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-terazolium-5-carboxanilide (XTT) assay (Sigma) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... Proliferation at corresponding time points was assessed by MTT (Methylthiazolyldiphenyl-tetrazolium bromide) assay according to the manufacturer’s recommendations (Sigma). For each time point ...
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bioRxiv - Cancer Biology 2022Quote: The PDX tissues were lysed in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitor cocktail (Sigma-Aldrich: #P8340). The protein concentrations were calculated by BCA assay ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... as for the “Group two-choice assay” except using a single 3 cm diameter Petri dish containing 0.67% agarose and 150 mM sucrose (Sigma) onto which a non-accessible container covered with a fabric gauze and a perforated cap of a 15 ml Falcon tube (diameter 1 cm ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were assayed for secreted chemokines and cytokines using the Milliplex 38-plex Human Cytokine/Chemokine Assay (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
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bioRxiv - Microbiology 2022Quote: ... Cells from these cultures were then used for batch culture growth kinetic assays in Tibbles–Rawling Minimal Medium13,30 (TR medium) containing 0.1% (weight/volume) alginate (Sigma Aldrich). Sterile alginate solution was prepared using nanopure water and filter sterilized using 0.40 μm surfactant-free cellulose acetate filters (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... was propagated in Vero cells and titers were determined by plaque assay on Vero cells using 1% methylcellulose (Sigma)(Stojdl et al. ...
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bioRxiv - Immunology 2022Quote: Cytokines were measured in duplicate plasma samples using an HCYTMAG-60K-PX41 Premixed 41 Plex - Immunology Multiplex Assay (Millipore). The results were subsequently analyzed using GraphPad Prism Version 9.2.0 software (San Diego ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 cells (for proliferation assay) or 1000 cells (for 3D uptake) per 20μl drop containing 4.8mg/ml methylcellulose (Sigma-Aldrich) and 20μg/ml soluble collagen I (BioEngineering ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed twice with 100μL/well HBSS prior to addition of 80μL/well assay buffer (HBSS (Sigma H8264) + 0.1% BSA) ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were blocked for 1 h in AD before addition of assay samples for 1 h followed by incubation with 1/20,000 dilution Goat anti-human Fc-HRP (Sigma). All steps were conducted at 37°C and separated by three washes with PBS-T (0.05% Tween-20) ...
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bioRxiv - Cancer Biology 2024Quote: Proximity Ligation Assay (PLA) was performed using the Duolink® PLA reagents according to the manufacturer’s instructions (Sigma Aldrich). An EVOS M7000 imaging system (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: To assess localisation immunofluorescence assays were performed as described previously (31) using anti-HA (1:500, anti-rat,Sigma), anti-MYS (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... reflecting cell-number and -viability) was assessed using the MTT [Thiazolyl Blue Tetrazolium Bromide] assay (Sigma/Merck, M5655-500mg) according to manufacturer’s instructions and sigmoidal dose-response curves were generated to calculate the mean IC50 values of each drug that were used in the subsequent study ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lysates were tumbled for one hour at 4°C with 20uL of RAS assay reagent (Millipore; #14-278).
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bioRxiv - Cell Biology 2023Quote: ... All following steps of the proximity ligation assay were performed according to suppliers’ instructions (Duolink® PLA; Sigma-Aldrich). Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436 ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.1.1.27) activities and concentrations of pyruvate and lactate were measured in samples via enzymatic assays (all from Sigma-Aldrich). All assays have been optimized for the current sample types as explained in the previous studies [37,48] and specific protocol settings for each assay can be found in [37] ...
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bioRxiv - Physiology 2023Quote: ... Triacylglycerol concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triacylglycerol Agent solution (Sigma Triglyceride and Free-Glycerol reagents ...
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bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Experimental evolution and all root colonization assays were performed in half strength Murashige and Skoog (MS) basal medium (2.22 g L−1, Sigma) supplemented with 0.05% glycerol ...
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bioRxiv - Immunology 2023Quote: Mouse sera were diluted 1:100 in an assay buffer of 0.05% PBS-T supplemented with 3% (w/v) BSA (Sigma) and transferred into a 96-well plate ...
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bioRxiv - Microbiology 2023Quote: Cellular cytotoxicity was measured by a colorimetric assay that makes use of thiazolyl blue tetrazolium bromide (MTT: Sigma, USA). Sub-confluent monolayer culture of RAW264.7 ...
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bioRxiv - Molecular Biology 2023Quote: ... The plate was incubated for 4 h at room temperature and the assay then harvested onto a GF/C filter plate (PE) that had been presoaked in 0.3% polyethyleneimine (Sigma) using a Packard Filter Mate Harvester ...