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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and RANK-L (100 ng/ml) for 4 to 6 days followed by staining for Tartrate-resistant acid phosphatase (TRAP) using a kit (Sigma) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Cell Biology 2021Quote: NCMs were seeded at a density 4 or 8 × 105 cells/well in 6-well plates coated with 0.01% of collagen (Sigma-Aldrich) and cultured in a medium containing DMEM/Medium199 (4:1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Next the slides were mounted with a coverslip using Mowiol (6 g glycerol, 2.4 gr polyvinylalcohol 4-88 (Sigma, 81381), 6 ml MQ and 12 ml 0.2 M Tris HCL pH 8.5).
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bioRxiv - Physiology 2021Quote: ... 6 × 105 cells were seeded on 6-well multiwell plates and counted every two days from day 4 to day 14 using Trypan blue (Sigma) and a Neubauer chamber.
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bioRxiv - Developmental Biology 2021Quote: ... inactivated with 10 µg/ml mitomycin C and seeded at 4 × 105 per 6 cm dish) in DMEM/F12/KSR medium (D-MEM/F12 (Sigma) supplemented with 20% KSR additive ...
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bioRxiv - Neuroscience 2021Quote: ... a pool of 4 brains was used (n = 6) and mixed with 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). The homogenate was centrifuged at 2400 g for 10 min at 4 °C and the supernatants were collected for the analyses of the following parameters ...
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bioRxiv - Biochemistry 2022Quote: ... a total of 0.4 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) and cells were fixed 4 days later ...
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bioRxiv - Immunology 2022Quote: ... Progenitors were spinfected in 6-well plates at 23-32°C for 30 minutes at 1000 x g with 4 µg/mL polybrene (Sigma) and 1 µM β-estradiol at 0.25 × 106 cells/mL for single gene knockouts ...
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bioRxiv - Neuroscience 2022Quote: ... mito-tempol[23] (25 μg, Sanbio) and oligodeoxynucleotide (3 μg/μl day 4, 5 and 6 after carrageenan, Sigma-Aldrich), were performed under light isoflurane anesthesia as described.[8a ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT cells were plated in 10-cm dish with culture medium containing only blasticidin S following co-transfection with Flp recombinase expressed plasmid pPGKFLPobpA and tetracycline-responsive plasmid pcDNA5FRT/TO containing TDP-43 Q331K or pcDNA5FRT/TO only as isogenic sham control in the ratio 6:4 using PEI (polyethyleneimine; Sigma) transfection method ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... i.p.) young adult (4-week-old) C57BL/6 male mice and transferred to ice-cold DMEM/F12 media (Sigma Aldrich) in 6 well plates ...
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bioRxiv - Cell Biology 2023Quote: ... Gene mutation was induced 4 days after organoid passage during 6 days with 400 ng/ml doxycycline (D9891, Sigma-Aldrich). When indicated ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting submucosa was cut into 6 inch pieces and decellularized using 4% alcohol/0.1% peracetic acid (v/v, Sigma-Aldrich) and washed thoroughly with 1X PBS and Type 1 water followed by lyophilization and comminuted into an injectable particulate form via cryogenic milling and sieving through 425 µM pore sieve ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted and purified mGlu5-5M receptor and mGlu5-5M_W785A receptor mutant bound to quisqualate and PAM were concentrated to 5-6 mg/ml using Amicon Ultra-4 centrifugal concentrator 100 kDa (Millipore) for cryoEM grid preparation and analysis.
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bioRxiv - Immunology 2024Quote: ... Hyde) were cultured for 4 days at 37ºC in BSK-II media and complete with 6% rabbit serum (Millipore Sigma) at pH of 7.6 ...
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bioRxiv - Systems Biology 2024Quote: ... and positive control using AP-MS lysis buffer 4 (see Materials, 0.5 ml) including 6 µg of BAP-FLAG fusion protein (Sigma, P7457). Tubes were incubated for 1 h at 4°C using an orbital rotator ...
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bioRxiv - Cancer Biology 2024Quote: ... CreER mice were induced at ∼4 weeks of age with 8mg/40g (obese Lepob/ob) or 6 mg/40g (non-obese Lepob/+) of tamoxifen (Sigma) in corn oil via oral gavage for 4 consecutive days ...
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bioRxiv - Physiology 2022Quote: ... Diet composition: Supplemental Tables 1A and 1B) and L-nitroarginine methyl ester (L-NAME, 1 mg/ml, Cat #N5751, Sigma Chemical, St Louis, MO) in the drinking water ...
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bioRxiv - Developmental Biology 2024Quote: ... On the 7th day 1 µM Senexin B (SenB, Senex Biotechnology, Columbia, SC) or 1 µM 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Microbiology 2022Quote: ... diluted in DMSO and 6-Thioguanine (6-TG) (Sigma-Aldrich) diluted in 1M NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... and glucose-6-phosphate dehydrogenase (G-6-PDH; Sigma-Aldrich). For enhanced chemiluminescence detection (ECL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Dextran T-500 (Sigma-Aldrich; 6 mL, 6% in saline) was added to the cells and filled up to 50 mL with 0.9% saline ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/mL 6-animopenicillanic acid (6-APA) (Sigma-Aldrich), 5 mg/mL 7-aminocephalosporanic acid (7-ACA ...
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bioRxiv - Neuroscience 2024Quote: ... The neurotoxin 6-hydroxydopamine-HBr (6-OHDA; Sigma Aldrich AB) was dissolved in 0.1% ascorbate-saline at the concentration 4 µg/µl (freebase) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... airway mechanics were measured by challenging pigs to increasing concentrations of methacholine hydrochloride (MeCh) (0, 0.5, 1, 2, 4, 8, 16, and 32 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) via an Aeroneb™ nebulizer (Aerogen Ltd ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by the addition of 28 μl of a stop reaction mixture: 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM 3-isobutyl-1-methylxanthine (IBMX) (Sigma, Germany #28822-58-4). After 48 h the induction medium was replaced by the differentiation medium containing DMEM/Ham’s F12 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hour and 1 μg/ml Laminin2020 for 4 hours (Sigma). Cells were cultured on NES culture medium contained DMEM/F12+GlutaMax (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... and 4-(Methylnitrosoamino)-1-(3-pyridinyl)-1-butanone (NNK) (78013, Millipore-Sigma) (0.41 mg/dose ...
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bioRxiv - Bioengineering 2023Quote: ... and 4-(Methylnitrosoamino)-1-(3-pyridinyl)-1-butanone (NNK) (78013, Millipore-Sigma) (0.41 mg/dose ...
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bioRxiv - Plant Biology 2020Quote: ... the first aliquot was derivatized using cysteamine (0.25 M; pH 8; 1h; room temperature; Sigma-Aldrich), the second aliquot was immediately further processed as following ...
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bioRxiv - Immunology 2021Quote: ... for 1h with or without a pretreatment with 20 μM MCC950 (Millipore Sigma, Cat. no: 538120) for 1h prior to activation ...
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bioRxiv - Immunology 2021Quote: ... for 1h followed by NLRP3 activation with 20 μM nigericin (Sigma-Aldrich, Cat. no: N7143-5MG) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... with prior 1h incubation of the biotinylated oligonucleotide with the anti-G4 antibody BG4 (Merck Millipore).
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bioRxiv - Bioengineering 2020Quote: ... in 1x PBS and exposed for 1h to a 3% Bovine Serum Albumin (BSA, Sigma Aldrich) blocking solution in 1x PBS.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was administered 30 minutes before the stimulus with NMDA (30μM for or 1h; Sigma–Aldrich, #M3262), dexamethasone (1 μM for 1h or 24h ...
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bioRxiv - Genomics 2021Quote: ... the membranes were incubated for 1h with the secondary antibody goat anti-rabbit-HRP (Sigma, G9545) 1:10,000 in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... A carboxylated indene standard (1H-Indene-3-carboxylic acid) was purchased from Sigma-Aldrich (catalog #MNO000013). Hexanes and acetone (Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... secondary antibody was incubated at room temperature for 1h followed by DAPI nuclear staining (Sigma, Germany). Immunostainings were examined with an immunofluorescence microscope (Leica) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a blocking step for 1h with 5% normal donkey serum (NDS) (D9663, Sigma Aldrich) in 0.1% PBS-Triton X-100 (PBS-T) ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated for 1h at RT in blocking buffer containing 10% goat serum (Sigma, G9023) with 0.03 % triton X-100 in PBS and 1:50 Affini Pure Fab Fragment Donkey Anti-Mouse IgG (Jackson Immuno Research Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed for 1h using 5% dried milk or Bovine Serum Albumin (Sigma-Aldrich #A9647) in TBS-T (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were blocked for 1h in PBS-Triton (0.2% Triton X-100, Sigma-Aldrich, #T-9284) with 10% Normal Donkey Serum (NDS ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected brains were post-fixed for 2 h at 4°C in 4% PFA and cryoprotected in successive solutions of 15% and 30% sucrose (Sigma-Aldrich, Cat# S0389) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Vital dye staining of lateral line hair cells of the 4 dpf embryos was performed using DASPEI (2-(4-(dimethylamino) styryl)-N-ethylpyridinium iodide) (Sigma-Aldrich, USA, D0815), 0.8 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
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bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% cyclocreatine (w/v) (2-imino-1-imidazolidineaceticacid) (Sigma #377627) was supplied in drinking water ad libitum for one month.