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Citations for Millipore Sigma :
2251 - 2300 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and mouse GAPDH monoclonal antibody for protein loading controls (Sigma, G8795). Anti-mouse IRDye680RD and anti-rabbit IRDye800CW (Licor ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were transferred onto PVDF membranes (Immobilon; EMD millipore, Darmstadt, Germany) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cycloheximide (inhibitor of protein synthesis) was used at 10µg/mL (Sigma) and added 30 min prior to cytokine treatment of neutrophils.
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bioRxiv - Microbiology 2020Quote: ... We purified the protein using anti-FLAG M2 Affinity Gel (Sigma) according to manufacturer’s protocol and removed the FLAG tag and His6-tag with TEV digestion followed by reverse nickel affinity purification and sizeexclusion chromatography on a Superose 6 Increase column (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Protein extraction was performed in the presence of 2% SDS (Sigma) and 4 M acetic acid (Fisher ...
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bioRxiv - Immunology 2020Quote: ... Proteins were transferred to Immobilon-FL PVDF transfer membrane (Millipore Sigma) using NuPAGE transfer buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... After transfer of the proteins onto a nitrocellulose membrane (GE10600001, Sigma) for 1 h at 100mV ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein bands were visualized with Immobilon Chemiluminescent Alkaline Phosphatase substrate (Millipore) and detected in a VersaDoc Model 4000 CCD camera system (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bradford dye for protein estimation was purchased from Sigma-Aldrich (USA). All the primers were synthesized by GCC Biotech (India ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with mouse anti-FLAG monoclonal (Sigma, 1/10000), rabbit anti-Rad51 polyclonal (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein was transferred to an Immobilon-P PVDF membrane (EMD Millipore). After transfer ...
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bioRxiv - Genetics 2021Quote: ... Proteins were then transfered to an Immobilon-E PVDF membrane (Millipore), which was then washed with 1× PBS containing 0.05% Tween 20 (three washes ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane (Millipore, Bedford, MA, USA). Membranes were blocked with 5% non-fat dried milk in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were spiked with ubiquitin (Sigma-Aldrich BioUltra, 5 nmol), a small subunit protein native to eukaryotes ...
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bioRxiv - Biophysics 2021Quote: ... 2.5μL of Precision Plus Protein™ Dual Color Standard from Sigma was used.
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred onto an Immobilon-P polyvinylidene difluoride membrane (Millipore) using a Bio-Rad Criterion Blotter ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were then transferred to a nitrocellulose filter membrane (Millipore) and were incubated overnight with indicated primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reconstituted in sterile DMSO (Millipore-Sigma, Burlington, MA, USA) to stock concentrations of 10 mM ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon®-P, Millipore) with a semi-dry transfer system (Trans-Blot® SD ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were mixed with 2x Laemlli sample buffer (S3401, Sigma) and heated at 95°C for 10 minutes prior to SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates were collected using Radio-Immunoprecipitation Assay (RIPA) buffer (Sigma), supplemented with protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... After protein quantification by the bicinchoninic acid colorimetric assay system (Sigma), 1mg protein samples were used for HDAC3 immunoprecipitation using four microliters of HDAC3 antibodies (Cell Signalling ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: ... and the resolved proteins were then transferred to PVDF membrane (Millipore). The membrane was blocked using 5% skimmed milk and then immunoblotted with primary antibodies at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with trypsin (TPCK Treated; Sigma; Cat. # 4352157). iTRAQ labeling of the peptides in each sample was performed using the iTRAQ Reagents Multiplex kit (AB Sciex) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was treated with 10 U PreScission protease (Sigma) at 4°C for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were concentrated using a 10 K Amicon (Merck-Millipore), flash-frozen in liquid nitrogen and stored at −80°C until use.
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were recovered by binding to protein A/G agarose (Millipore), and the eluted DNA fragments were used directly for qPCR or subjected to high-throughput sequencing (ChIP-Seq ...
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bioRxiv - Cell Biology 2022Quote: ... and phosSTOP if phosphorylated proteins were being investigated (Sigma-Aldrich, 04906845001)] ...
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bioRxiv - Cell Biology 2022Quote: ... After protein transfer membranes were incubated in Ponceau solution (Sigma-Aldrich) to confirm even transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined by Bradford assay kit (Sigma-Aldrich, B6916). Lysates were mixed with 5 × sample buffer (250 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... stained with 1:200 mouse anti-ubiquitinylated proteins (Sigma #04-263) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were electro-transferred to a polyvinylidene fluoride membrane (Millipore) using Turbo-Blot Turbo transfer system (settings ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Microbiology 2022Quote: ... affinity purified IcsAp protein was mixed with 0.1 mM DSP (Sigma) in PBS and incubated at room temperature for 30 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... resuspended and lysed in 200 μL Bugbuster protein extraction reagent (Millipore). Following another centrifugation ...
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bioRxiv - Plant Biology 2022Quote: ... the proteins were transferred on a PVDF membrane (Immobilon-P, Millipore) using a wet blotting system ...
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bioRxiv - Plant Biology 2022Quote: ... samples were incubated with 25 μl of protein A beads (Millipore) for 2 h in cold room (100 μL were collected as input) ...
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bioRxiv - Neuroscience 2022Quote: ... Resulting protein lysates were precipitated with trichloroacetic acid (TCA) (Sigma, T0699). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-phosphoepitope SR proteins clone1H4 (Millipore, cat#MABE50, 1:2,000 dilution) were used as primary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as measured by a BCA protein assay kit (Sigma-Aldrich, USA).
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bioRxiv - Plant Biology 2022Quote: ... Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore, USA). The membrane was incubated with rabbit anti-C ...
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bioRxiv - Molecular Biology 2023Quote: ... resolved proteins were transferred onto Immobilon-P PVDF membranes (Millipore Sigma) using a Bio-Rad Trans-blot® semi-dry transfer cell ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were then reduced with 10 mM dithiothreitol (D9779, Sigma), followed by alkylation with 30 mM Iodoacetamide (I1149 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was then transferred onto Infrared PVDF membranes (Millipore, Burlington, MA) and membranes were blocked in Immobilon Blok Solution (Millipore ...
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized using a chemiluminescence (ECL) kit (Millipore, MA, USA). Target protein expression was normalized to β-actin (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... Target protein expression was normalized to β-actin (Sigma-Aldrich, #2066) or vinculin (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was pre-cleared with Protein G agarose beads (Sigma) and anti-rabbit IgG (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... were firstly incubated with the Protein-G agarose (Millipore, 16-266) for 2 hrs ...