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Citations for Millipore Sigma :
2251 - 2300 of 2558 citations for PTH Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were blocked in a blocking solution including 1% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... The slides were cooled at room temperature and immersed in a blocking solution (PBS containing 3 % bovine serum albumin, BSA, and 0.01 % Triton X-100, all from Sigma, St. Louis, MO) for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and samples were incubated in blocking solution (3% powdered nonfat milk in PBS + 0.1% IGEPAL CA-630 (Sigma-Aldrich, 18896, lot 1043)) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were incubated in blocking buffer with primary antibody (Rabbit α-β-III Tubulin, Millipore Sigma T2200-200UL, 1:1000, RRID: AB_262133) overnight at 4°C without agitation ...
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bioRxiv - Neuroscience 2021Quote: The other two remaining free-floating brain sections were washed in 0.1% PBS with 0.1 % Triton A-10 (PBS-T) and incubated in blocking solution (1 h at room temperature; PBS-T with 10% donkey serum, Sigma Life Science). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each and 100 μl of blocking solution containing goat anti-mouse IgG (Sigma 1:3,000) was added to each well and incubated at 1 h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was probed overnight at 4°C with blocking buffer containing rabbit anti-Extramacrochaetae antibody and an antibody to β-actin (Sigma-Aldrich). The membrane was then washed four times with 1X PBST for five minutes each ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were incubated overnight with the following antibodies diluted into blocking solution: chicken anti-GFP (1:1,000; AB3080; Millipore Bioscience Research Reagents), rabbit anti-RFP (1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 1 hour at RT with the following antibodies diluted in blocking buffer: fibronectin (Sigma-Aldrich, St. Louis, MO) and α-smooth muscle actin (α-SMA ...
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bioRxiv - Neuroscience 2021Quote: ... for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml, Millipore, MA, USA). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... These sections were placed in a blocking buffer (2% normal donkey serum and 1% bovine serum albumin in PBST; Sigma A7906-100G) for 30min ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated with primary antibodies in blocking buffer in a humidified chamber for 1 h: anti-FLAG M2 (1:2000 dilution, Sigma Aldrich, F3165) and an endogenous compartment marker antibody from rabbit (Supplementary Table 21 for list of antibodies used) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and then blocked for 1 h at 37 °C with blocking buffer containing 1% BSA (Sigma-Aldrich, Taufkirchen, Germany) and 0.15M sucrose (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue samples were blocked for 1 hour at room temperature in blocking solution (8% BSA solution) and then transferred to a carrier solution (8% BSA solution) containing diluted antibodies: GOT2 (Sigma-Aldrich HPA018139), GOT2 (Thermo Fisher PA5-77990) ...
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bioRxiv - Microbiology 2022Quote: ... and blocked for 3 h at 4°C in 60 ml blocking buffer composed of TBST buffer supplemented with 5% (w/v) BSA (Sigma Life Science). During the blocking stage ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated with the primary antibodies in a blocking solution for 5 days at 4°C: mouse anti-GFAP (G3893, Sigma, 1:300), mouse anti-HSP70 (MA3-028 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 hour of blocking with 5% horse serum in PBS was followed by incubation with anti-FLAG primary antibody (1:500, Sigma Aldrich F3165) in 3% horse serum in PBS for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... free floating sections were incubated with mouse anti-FLAG clone M2 antibodies (1:2000 in the donkey serum blocking solution, Sigma-Aldrich F1804), overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and samples were incubated in blocking solution (3% powdered nonfat milk in PBS + 0.1% IGEPAL CA-630 (Sigma-Aldrich, 18896, lot 1043) for 10 minutes minimum at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1 hr with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidity chamber at RT for 1 hour with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Neuroscience 2021Quote: ... and iii) blocking with 5% Bovine Serum Albumin (BSA) and 0.5% Triton-X in Phosphate Buffer Saline (PBS, Sigma-Aldrich ref. P4417) for 30 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by incubation in blocking solution and primary antibody against ChAT (Millipore, AB144P, goat anti-ChAT, 1:500 v/v) for five nights at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... After incubating overnight in blocking solution containing a primary antibody (polyclonal rabbit anti-tyrosine hydroxylase; 1:1000 dilution; Millipore; catalog no. 657012), sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG ...
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bioRxiv - Pathology 2020Quote: ... the coverslips were incubated in blocking buffer for 45 min at room temperature with antibodies against LC3-II (Sigma Aldrich, 1:200), LAMP2 (DSHB ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibody was diluted 1:100 in blocking buffer (rabbit-α-LAMP1: 1:100; rabbit-α-mTOR (HPA071227, Sigma-Aldrich Co. LLC): 1:12.5 ...
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bioRxiv - Genetics 2020Quote: ... rinsed and then pre-incubated with blocking solution (10% Normal Goat Serum (Brunschwig, #G-S-1000) and 2% Bovine Serum Albumin (Sigma-Aldrich, #A7906) in PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 48 hr in blocking buffer containing a rabbit anti-c-Fos antibody (1:3000, ABE457, Millipore, Bellerica, MA). After washes in TNT (0.1 M Tris-HCl ...
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bioRxiv - Pathology 2021Quote: ... Antibody solution consisted of blocking solution with rabbit anti-Iba1-635 (Wako, cat. # 013-26471) at 1:400 and DAPI (Sigma, cat. # D9542) at 10 µg/mL.
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM reporter probe DNA and 30 μl of 100 μM blocking probe sequences (PolyT10) thiolated DNA were activated by using 10 mM Tris (2-carboxyethtl) phosphine (TCEP, Sigma-Aldric, USA) freshly prepared ...
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bioRxiv - Immunology 2020Quote: ... Env protein bound beads were further incubated with blocking buffer (PBS [pH 7.4], 0.1% bovine serum albumin [BSA; Sigma], and 0.05% Tween 20) at 37°C to block the unbound sites and the antigenic integrity of Env proteins were assessed by examining their ability to bind to different mAbs by flow cytometry (FACSCanto ...
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bioRxiv - Biophysics 2022Quote: ... Solutions of primary antibodies were prepared in blocking solution using the following dilutions: mouse antibody against SRRM2 (1:2000, Sigma Aldrich, #S4045), mouse antibody against SRSF1 (1:250 ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized in ice-cold methanol by dipping the coverslip in it 20 times followed by blocking in 3% BSA (Sigma-Aldrich; A9647) in PBS for 15 min (Figures 2 A-H and S1) ...
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bioRxiv - Cell Biology 2022Quote: Embryos were incubated in 600 μl of blocking buffer (PBS, 0.01% Triton X-100 and 10% non-immune donkey serum (Sigma cat # D9663-10ml) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... both HeLa cells and cortical neurons were permeabilized after PFA fixation with 100% methanol chilled at −20℃ for 20 minutes followed by 1 h blocking with PBS containing 3% BSA (Sigma-Aldrich; A9418). Cells were stained with antibodies against SIN capsid ...
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bioRxiv - Neuroscience 2022Quote: ... Permeabilization of the membrane and blocking of unspecific binding sites were performed in one step by adding 0.5% Triton X-100 (Sigma-Aldrich, Buchs, Switzerland) to the blocking solution (PBS - Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cells were quenched in 0.1 M glycine in PBS and then permeabilized for 30 min in a blocking solution of 2 % normal goat serum (Sigma-Aldrich, # G9023) and 0.1 % Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma, X100] in 1xPBS) and then with primary antibodies for 2-3 h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... an overnight incubation was performed to label the peroxisomes with anti-PMP70 (Cat#P0497, Sigma-Aldrich; 1:1000 dilution in blocking solution). The slips were washed three times with PBS before the complementary anti-rabbit secondary antibody was added (Cat#A-11034 ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated overnight with retronectin solution (32 µg/mL in PBS, WEHI) at 4℃ followed by blocking with 2% bovine serum albumin solution (Sigma-Aldrich, #A1595) in PBS at 37℃ for 30 min prior to coating with viral supernatant ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by two additional PBS-T washes and then blocking - for 3 h at room temperature or overnight at 4°C - in BSA (Bovine Serum Albumin, Sigma-Aldrich #A2153) 3% in PBS-T ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich] ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then incubated for 2-hour incubation with a blocking solution at room temperature and subsequently incubated with anti-digoxigenin-Rhodamine (Anti-digoxigenin-Rhodamine, Fab fragments, Sigma, Cat# 11207750910) (0.5μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells on coverslips were blocked with blocking solution (2% BSA/PBST) for 30 min and incubated with the primary antibodies anti-Flag (mouse, Sigma, M2, F1804), anti-PABPN1 (rabbit ...
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bioRxiv - Immunology 2022Quote: ... and the plate was blocked with 200 µL blocking solution (1% BSA [Sigma, #A9418-5G], 0.05% Tween [Sigma, #P9416-100ML], in PBS). The solution was removed and sample or standard curve (made with mouse IFN-β [PBL Biomedical Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Fixation was followed by blocking (1h, RT) with 3% bovine serum albumin (BSA/0.01% Tween™20/PBS) (Sigma, Cat#A-7906). Samples were incubated with primary antibodies (overnight ...