Labshake search
Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... Mouse anti-Flag and non-specific mouse IgG (4mg/ml; Sigma F7425; RRIB:AB_439687) were used for pull-down ...
-
bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-TRA1-60 and Mouse anti-TRA1-81 1/200 (both Millipore), Mouse anti-PAX6 ...
-
bioRxiv - Immunology 2021Quote: ... mouse IgG1 anti Golgin 97 (CST) or mouse anti IgG1 PDIA3 (Sigma-Aldrich) and mouse IgG2a anti-dsRNA J2 (Scicons ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse anti-mouse α-smooth muscle actin (αSMA) conjugated to Cy3 fluorophore (Sigma), and mouse anti-rat neural-glial antigen 2 (NG2 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Mouse anti-myc antibody (1 μg) or mouse IgG (1 μg) (EMD Millipore) were incubated with protein A/G Magna beads (20 μl ...
-
bioRxiv - Cell Biology 2023Quote: ... Mouse Vinculin (V9131) and mouse Flag (F3165) antibody were purchased from Sigma-Aldrich. Antibodies against METTL14 (26158-1-AP) ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse anti-FLAG (M2) and mouse anti-α-tubulin (clone DM1A) (Sigma-Aldrich); mouse anti-clathrin heavy chain (BD Biosciences) ...
-
bioRxiv - Molecular Biology 2024Quote: ... anti-HA Mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma-Aldrich). The membrane was washed thrice in PBST and incubated with HRP-conjugated secondary antibody (anti-rabbit and anti-mouse ...
-
bioRxiv - Cancer Biology 2020Quote: ... Antibodies for immunostaining include rabbit anti-human/mouse PKD-1 (4502371) and mouse anti-mouse α-smooth muscle actin(A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
-
bioRxiv - Biochemistry 2021Quote: ... programed for uniform mixing as previously described at a controlled temperature and modified with online automated desalting.13 Proteins were denatured in a solution of 35% v/v 2-2-2 Trifluoroethanol (TFE, Sigma), 40 mM Dithiothreitol (DTT ...
-
bioRxiv - Neuroscience 2023Quote: ... mice were anesthetized by intraperitoneal injection with Avertin (2, 2, 2-Tribromoethanol, Sigma-Aldrich, St. Louis, MO, USA; catalog #T4). Cardiac perfusion was performed with 0.1 M phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... the fluorescent dyes were removed by flowing 2 mL of the cleavage buffer containing 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; 646547, Sigma) with a 15-min incubation time in the flow chamber to cleave the disulfide bond linking the dyes to the readout probes ...
-
bioRxiv - Developmental Biology 2023Quote: ... The following factors were applied to experimental BPs in culture at the specified concentrations: 2-Deoxyglucose (2-DOG) 2 mM (SIGMA), Sodium Pyruvate (NaP ...
-
bioRxiv - Immunology 2023Quote: ... T cells transduced with the anti-hCD19 CAR construct 42 were cultivated for additional 2 days in presence or without 2 mM 2-DG (Sigma) before adoptive transfer into MC38-hCD19 tumor-bearing mice.
-
bioRxiv - Biophysics 2023Quote: ... different amounts of freeze dried GelMA macromer were dissolved in DPBS containing 0.5% (w/v) 2-Hydroxy-4’-(2-hydroxyethoxy)-2- methylpropiophenone (Sigma-Aldrich) as photoinitiator ...
-
bioRxiv - Immunology 2020Quote: ... for 2 days and then just IL-2 (20ng/ml) (Sigma-Aldrich) for a further 5 days ...
-
bioRxiv - Developmental Biology 2021Quote: ... the tissues were processed to 2’-2’-thio-diethanol (TDE, Sigma, 166782) for clearing and mounting ...
-
bioRxiv - Immunology 2022Quote: ... 5 μM oligomycin and 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich) were injected to each well after 18 ...
-
bioRxiv - Immunology 2021Quote: ... 2-Deoxy-D-glucose (2-DG) was obtained from Sigma Aldrich (D8375) and used at 0.32 mM ...
-
bioRxiv - Genomics 2020Quote: ... Substrate solution (2 mM ABTS, Sigma A9941; 2 mM hydrogen peroxide, Sigma H1009 ...
-
bioRxiv - Cell Biology 2021Quote: ... The last injection was supplemented with 2-deoxyglucose (2-DG, Sigma, D8375) to obtain the ECAR background value due to nonglycolytic acidification ...
-
bioRxiv - Immunology 2019Quote: ... 2’-Deoxyadenosine (dAdo) and 2’-Deoxycytidine (dCyt) were purchased from Sigma-Aldrich Japan (Tokyo ...
-
bioRxiv - Immunology 2021Quote: ... For the last 2 h 2 µg/ mL Brefeldin A (Sigma-Aldrich) was added ...
-
bioRxiv - Immunology 2021Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... 55μM 2-mercaptoethanol (2-ME) supplemented with 50 U/ml Benzonase (Sigma). Aliquots derived from the same donors were combined and all samples were enriched for T cells by removing CD14 and CD19 expressing cells using a column-based magnetic depletion approach according to the manufacturer’s recommendations (Miltenyi) ...
-
bioRxiv - Immunology 2021Quote: ... cells were treated with 2-deoxyglucose (2-DG) 100 nM (Sigma, #D8375).
-
bioRxiv - Immunology 2020Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
-
bioRxiv - Biochemistry 2022Quote: ... 3-[2-(2-aminoethylamino)-ethylamino] propyl-trimethoxysilane (DETA) (Sigma-Aldrich, Hamburg, Germany) for microtubule immobilization ...
-
bioRxiv - Biophysics 2022Quote: ... and 2 µM 9-Amino-6-Chloro-2-Methoxyacridine (ACMA, Sigma-Aldrich) to establish a K+ gradient ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 µM epoxomicin and 50 µM (Z-LL)2-ketone (Merck Millipore) were added from stock solutions in dimethyl sulfoxide (DMSO) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1µM Oligomycin A and 50mM of 2-deoxyglucose (2-DG) (Sigma, D3179). For mitostress tests ...
-
bioRxiv - Bioengineering 2023Quote: ... 0.15 M 2-amino-2-methyl-1-propanol (AMP) (A65182, Sigma-Aldrich) in UPW ...
-
bioRxiv - Biophysics 2024Quote: ... and 3% (wt/vol) hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma, 410896) in ethanol ...
-
bioRxiv - Biochemistry 2019Quote: Insulin and amylin concentration in plasma were determinate by ELISA (Insulin, Cat # EZRMI-13K and Amylin, Cat # EZHA-52K, Merck Millipore) following manufacturer manual ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were lysed and processed for cell death detection ELISA assay by following the manufacturer’s instruction (#11774425001, Millipore Sigma). At the end of the assay ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were then rewashed three times with PBST before the addition of 3,3’,5,5’-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, UK) and incubated for 12 minutes in the dark at RT ...
-
bioRxiv - Immunology 2022Quote: Stable knockdown of host proteins in THP1 cells was carried out by transduction with lentivectors expressing individual shRNAs (pLKO.1, 400 ng p24Gag (as measured by ELISA) per 1 × 106 cells) in the presence of polybrene (Millipore). Cells were washed and cultured for 5-7 days in the presence of puromycin (2 µg/ml ...
-
bioRxiv - Immunology 2019Quote: ... The peritoneal lavage fluids were collected and concentrated for ELISA analysis with Amicon Ultra 10 K filter (UFC900308) from Millipore. Peritoneal exudate cells were collected and analyzed by FACS.
-
bioRxiv - Microbiology 2020Quote: ... The ELISA plates were then blocked with 300 μL of blocking buffer (1% BSA and 10% bovine calf serum (BCS) (Sigma) in PBS at 37°C for 2 hrs ...
-
bioRxiv - Microbiology 2021Quote: ... and stored at −80°C until particle concentration assessment by p24 ELISA (Innogenetics) while the rest of the supernatant was concentrated by filtration on Centricon units (Centricon Plus-70/100K, Millipore) and frozen at −80°C for RNA extraction ...
-
Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsybioRxiv - Neuroscience 2023Quote: ... Lysates were removed and protein antibody-conjugates linked to the bottom of the plate were washed three times with 300 µL of ELISA wash buffer (100mM Tris buffered saline, pH8.2, 2mM MgCl2, 0.5% Tween-20 – all Sigma-Aldrich, Ireland). Protein was eluted by incubating wells in 2 X Laemelli buffer for 10 mins ...
-
bioRxiv - Immunology 2022Quote: ... antibody levels were detected using a secondary anti-murine IgG conjugated with alkaline phosphatase and the substrate for alkaline phosphatase yellow (pNPP) liquid substrate system for ELISA (cat. P7998, Sigma). After 30 minutes of incubation ...
-
bioRxiv - Microbiology 2021Quote: ... were fluorescently labelled by the addition of 2 μl PKH26 dye (1 mM stock concentration) from the MINI26 PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich) and mixed by pipetting ...
-
bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was then extracted from 2 mL of cell culture using a GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich, USA). DNA samples were subjected to an initial quality check using a Nanodrop spectrophotometer (Thermo Scientific ...
-
bioRxiv - Microbiology 2021Quote: Cell viability and cytotoxicity of antibiotics and peptides were determined against human corneal epithelial cells (HCE-2, CRL-11135, ATCC, Manassas, Virginia, USA) using cell-counting-kit-8 (CCK-8) assay (Sigma Aldrich, Merck Life Science UK Limited ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatant was removed and the cell pellet was resuspended in 500 μL Diluent C containing 2 μL PKH67 dye (PKH67 Green Fluorescent Cell Linker Kit, Sigma-Aldrich), incubated for 5 minutes at room temperature ...
-
bioRxiv - Genomics 2019Quote: ... with 2 mL of ice-cold EZ-Prep lysis buffer from the Nuclei EZ-prep Isolation Kit (Sigma, Cat #NUC-101). The tissue was dounced 25 times with Pestle A ...
-
bioRxiv - Immunology 2022Quote: ... 100-150 mg of snap-frozen tissue were mechanically homogenized in 750 µL of Lysis Solution/2-mercaptoethanol mixture and total RNA was isolated using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich). Reverse transcription for up to 2 µg of total RNA was performed using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Following primary antibody incubation coverslips were washed 2× in PBS and then the proximity ligation assay was carried out (Duolink In Situ Red Starter kit (Sigma Aldrich) according to the manufacturer’s instructions) ...