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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... The elutes were incubated with S-protein beads (EMD Millipore) overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: Protein samples were diluted in PBS pH=7.4 (Sigma-Aldrich). Neutravidin (NAv-Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were alkylated using 2-chloroacetamide (C0267-100G, Sigma-Aldrich) in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore, AmiconUltra 4 mL ...
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bioRxiv - Cancer Biology 2021Quote: ... at 1:56.6 dilution and Protein-A-agarose beads (Millipore, cat# IP02 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 mM protein phosphatase Inhibitor Cocktail I (Sigma Aldrich). Protein lysates were mixed with 5X SDS sample buffer and boiled then resolved on SDS-PAGE gels ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were incubated with protein G agarose (IP04, Millipore), Flag affinity agaros (A2220 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were reduced with 100 mM DTT (Sigma, D9163) for 35 min at 57°C and then handled according to the FASP (Filter Aided Sample Preparation ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to Immobilon-FL PVDF Membrane (Millipore #IPFL00010) in 1 x Tris/Glycine buffer (Biorad #161-0772 ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were reduced with 10 mM dithiothreitol (Sigma Aldrich, UK) in ammonium bicarbonate for 30 min at 37°C and alkylated with 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were precleared with protein A-Sepharose beads (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... protein aliquots were incubated with 10μg of free avidin (Sigma) per 1mg of input protein for 1h at 4°C with constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich, UK) for 30 min at 37°C and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Genetics 2021Quote: ... 1:250 rat anti-myelin basin protein (MBP) (Millipore MAB386); secondary antibodies were Alexa Fluor® conjugates from Life Technologies and used at 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and probed with appropriate dilutions of primary antibody followed by appropriate dilutions of horseradish peroxidase-conjugated secondary antibody ...
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bioRxiv - Biophysics 2020Quote: ... Buffer proteins were purchased from Sigma-Aldrich (St. Louis, MO), and salts were purchased from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2021Quote: ... Protein bands were detected with either anti-Flag (Sigma-Aldrich) or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Mpro proteins were concentrated to 25 mg/ml by Millipore’s ultrafiltration columns and a final concentration of 1 mM DTT was added and stored at −80°C.
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bioRxiv - Developmental Biology 2021Quote: For Hyaluronic Acid-Binding Protein (HA-BP, Sigma-Aldrich, 385911) immunofluorescence ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Protein expression was induced with 0.1% L-arabinose (Sigma-Aldrich). Expression took place for 16 h at 15°C and 220 rpm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Systems Biology 2020Quote: ... blots were stained with Ponseau S total protein stain (Sigma) prior to blocking at room temperature (5% skim milk ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were blotted on PVDF membrane (Millipore Immobilon-P, #IPVH00010) at 100 V for 1 h at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... The filtrates were then concentrated in protein concentration columns (Millipore CENTRICON YM-10 Centrifugal Filter Unit 2 mL 10 kDa ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were wet-transferred to PVDF membranes (Immobilon-P, Millipore), blocked in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore, #IPF00010) for 2 h at 30 V on ice ...
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bioRxiv - Biochemistry 2021Quote: Protein was labeled with Fluorescein Isothiocyanate (FITC, Merk, Sigma-Aldrich) according to the supplier’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2021Quote: Protein mixtures were precipitated with TCA (Sigma Aldrich, Cat# T0699) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fusion proteins were captured using V5 agarose beads (Sigma # A7345) that were pre-cleared overnight with PBS + 0.1% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-cleared with 40 μl Protein A agarose beads (Millipore), and then immunoprecipitated with specific antibodies against REST/NRSF (Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were reduced with 2μl of 0.2M dithiothreitol (Sigma) for one hour at 57 °C at pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were transferred to a nitrocellulose membrane (Merck Millipore). The membrane was then incubated with 5% BSA in Tris-buffered saline with tween 20 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred onto a nitrocellulose membrane (Millipore). The membranes were blocked using Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... lysed in BugBuster Primary Amine Free Protein Extraction Reagent (Novagen) supplemented with recombinant lysozyme ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein complexes were eluted with 3xFlag peptide (200ug/mL, Sigma) in 5 column volumes Buffer 2 for 1 hour at 4C ...
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bioRxiv - Systems Biology 2020Quote: ... proteins were transferred to Immobilon FL PVDF membrane (Merck-Millipore) using semi-dry transfer (25 V for 30 min.) ...
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bioRxiv - Microbiology 2020Quote: ... Protein quantification was performed using the Bradford Reagent (Sigma-Aldrich). Equal amounts of protein lysates were separated by SDS-PAGE and proteins were transferred to nitrocellulose filters ...
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bioRxiv - Molecular Biology 2020Quote: 20 μg protein was incubated in 5x Laemmli buffer (Sigma) at 95°C for 10 min and separated by a Mini-PROTEAN® TGX (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore), and blocked with 5% skim milk in TBS-0.1% Tween20 (TBST ...
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bioRxiv - Cell Biology 2020Quote: Proteins were extracted using RIPA buffer (Sigma-Aldrich, 20-188) supplemented with Complete Mini EDTA-free Protease inhibitor cocktail (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... and protein was precipitated by 10% TCA (trichloroacetic acid, Sigma) in acetone ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein concentration was determined using Bradford reagent (B6916, Sigma-Aldrich). For offline high pH reversed-phase (hpH RP ...
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bioRxiv - Biophysics 2021Quote: ... Purified protein was concentrated using Amicon Ultra-Centrifugal units (Millipore) and flash frozen for storage at −80°C or used immediately for experiments.
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bioRxiv - Immunology 2021Quote: ... The proteins were concentrated with Amicon (cutoff 10 kDa, Sigma) to achieve around 0.200 mg/mL of protein solution for mass spectrometry.
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bioRxiv - Microbiology 2021Quote: ... the protein solution was reduced with 10 mM DTT (Sigma) for 1 h at 37 °C and alkylated with 20 mM IAA(Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... proteins were transferred to Immobile FL PVDF membrane (EMD Millipore) and immunoblotting was performed with anti-FLAG M2 (F1804 ...
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bioRxiv - Cancer Biology 2021Quote: ... the proteins were visualized with Chemiluminescent HRP Substrate (Merk Millipore) and signals recorded using Bio-Rad ChemiDoc MP chemiluminescence imaging system (BIORAD).