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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the AgNP suspension was washed with deionized (DI) water by ultrafiltration through a membrane with pore size of 3 kDa (Amicon Ultracel 3 KDa Ultrafiltration Discs, Millipore, Billerica, USA) under nitrogen gas pressure (1 bar ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hrs followed by addition of 3 µM Asunaprevir (Apexio: BMS-650032) and 500 µM 3-Indoleacetic acid (Sigma Aldrich: I2886). Cells were treated with following inhibitors to inhibit indicated kinases ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were probed overnight at 4°C with anti-HA antibody (MBL, M180-3) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Sigma-Aldrich, G8795) or with anti-dystrophin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... A 0.5 M stock solution of inducer was prepared by solubilizing 3-methyl-benzoate (3-MB) (Sigma-Aldrich, St Louis, MO, USA) in 0.5 M NaOH.
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bioRxiv - Systems Biology 2022Quote: ... The His6-MBP or His6-MBP-14-3-3 concentrations on the resin were determined by pipetting 3×200 μL of diluted resin (corresponding to 3×15 μL of resin) into a filter plate (Millipore, Burlington, Massachusetts), filtering out the buffer by centrifugation (500g) ...
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bioRxiv - Genetics 2023Quote: ... Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 µg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Chemical compounds were first identified using the NIST library and later confirmed with co-elution of synthetic 4-methyl-3-heptanone (Pfaltz and Bauer M19160) and 4-methyl-3-heptanol (Sigma Aldrich M48309). Compounds eluting after 30 minutes were excluded from the analysis due to lack of volatility.
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bioRxiv - Developmental Biology 2023Quote: ... oocytes are first isolated from ovaries by short enzymatic digestion (3 mg/ml Collagenase I, Sigma-Aldrich, #C0130; 3 mg/ml Collagenase II, Sigma-Aldrich, #C6885 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were loaded onto a Supelco™ Discovery™ HS F5-3 (15 cm×2.1 mm, 3-μm) column (Sigma-Aldrich Co.) kept at 30 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 8029.3), and 0.5% sodium deoxycholate (AppliChem, A1531) in PBS supplemented with Phosphatase Inhibitor Cocktail 2 and Cocktail 3 (Sigma-Aldrich, P5726, P0044) and Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Immunology 2024Quote: ... The extract was reconstituted in a 500-μl solution of methanol/water (1:1) and filtered through a 3-kDa molecular filter (Amicon® Ultra Centrifugal Filter, 3 kDa MWCO, Millipore Sigma) at 4°C for 90 minutes to remove proteins ...
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bioRxiv - Developmental Biology 2024Quote: Back skin from E15.5 mouse embryos (3 control and 3 Prmt5 cKO embryos) was dissected and immediately transferred to ice cold DEPC-PBS (Millipore Sigma, D5758). RNA was isolated using the RNeasy Fibrous Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Colony growth was assessed by crystal violet staining after 2-3 weeks (HA1ER/HA1E: 3 weeks, For this, wells were fixed and stained with 0.1% crystal violet (Sigma-Aldrich, cat. C6158) and 10% ethanol for 30min ...
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bioRxiv - Developmental Biology 2024Quote: The SHMT2 gene was cloned from cDNA of HeLa cells using primers 5’-GCCCATATGGCCATTCGGGCTCAGCAC-3’ (forward) and 5’-GCCCTCGAGATGCTCATCAAAACCAGGCA-3’ (reverse) and subcloned into the pET41a vector (Novagen, WI, USA) at restriction enzyme sites of NdeI and XhoI ...
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bioRxiv - Immunology 2022Quote: ... The pseudotyped virus-mAb mixture were then overlayed onto target cell lines in the presence of 5 μg/ml polybrene (Sigma Aldrich, catalog no. TR-1003-G) and analyzed 48 h post-infection ...
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bioRxiv - Cancer Biology 2023Quote: ... at 1.5 × 105 cells per well and infected with 100-300μl of low-titer virus in the presence of infection mix (1/10 dilution of polybrene [10 mg ml−1 Sigma; 107689-100MG in PBS] in FBS[-]), by centrifuging plates at 1,100 g for 30 min at 37 °C in a Thermo Heraeus Megafuge 40R centrifuge ...
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bioRxiv - Genomics 2024Quote: ... Cells were infected with 100-300 µl of low-titer virus in the presence of the infection mix (1/10 dilution of polybrene [10 mg/ml Sigma; 107689-100MG in PBS] in FBS[-]), followed by centrifugation of the plates at 1,100 g for 30 minutes at 37 °C in a Thermo Heraeus Megafuge 40R centrifuge ...
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bioRxiv - Neuroscience 2021Quote: ... and Pellet Paint Co-precipitant (Merck Millipore #69049-3). Genomic DNA traces were removed with DNA-free DNase Treatment and Removal Reagents (FisherScientific #AM1906 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μM 3-Isobutyl-1-methylxanthine (IBMX, Sigma, #I7018) was supplemented to the media for the indicated time ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% of phosphatase inhibitor cocktail 3 (Sigma-Aldrich) was added to the adherent cells and permitted to incubate on ice for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides carrying a 3’ Cy5 fluorophore (purchased from Sigma) were ligated subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich), for 1 hour at 4°C on rotation ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated in 3% H2O2 (Sigma-Aldrich, Shanghai, China) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 3-methyl-2-oxovaleric acid sodium salt (ketoisoleucine, Sigma) or a combination of all three (BCKAs) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pretreated with 3 μM FTY720 (Sigma-Aldrich) for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... with 3 % bovine serum albumin (BSA, Sigma-Aldrich # A7906) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... ICC blocking solution (3% w/v BSA (Sigma-Aldrich), 3% v/v goat serum (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... and Phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich), and incubated 10 min on ice ...
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bioRxiv - Immunology 2021Quote: ... in the presence of Monensin (3 µM, Sigma Aldrich) for 4 h ...
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bioRxiv - Genetics 2021Quote: Cell fixation with 3% formaldehyde (Sigma-Aldrich, Cat. F8775) was performed as described in Dauban et al ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1 mM 3-Isobutyl-1-methylxanthine (IBMX; Sigma-Aldrich), 0.1 mM 8-Bromoadenosine 30,50-cyclic monophosphate sodium salt (cAMP ...
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bioRxiv - Cancer Biology 2020Quote: ... and active caspase-3 (1:1 000, Millipore, AB3623). Three fields of view for each slide were imaged using an Olympus BX43 Upright Microscope and cells were manually counted and scored using ImageJ Software.
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bioRxiv - Plant Biology 2022Quote: Indole-3-acetic acid (IAA, 10 μM; Sigma-Aldrich) and (+/-)-abscisic acid (ABA ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich), and sonicated using a fine probe (0.5-sec pulse at an amplitude of 20% ...
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bioRxiv - Neuroscience 2021Quote: ... 3’-diaminobenzadine substrate solution (0.5mg/ml; D5637, Sigma Aldrich) in 0.01% H2O2 in PBS was determined for each antigen ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl 3% (w/v) λ-Carrageenan (Sigma-Aldrich) in 0.9% sterile NaCl solution (saline ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl 3% (w/v) λ-Carrageenan (Sigma-Aldrich) in PBS 1X was injected into the mouse left hind paw using a Hamilton syringe ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit polyclonal anti-GluR2/3 (1:200, #AB1506, Millipore), mouse monoclonal anti-calretinin (1:750 ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich) and sonicated using a fine probe (0.5-sec pulse at amplitude of 20% ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich). After sonication using a fine probe [(0.5-sec pulse at an amplitude of 20% ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4) containing 3% bovine serum albumin (BSA; Sigma) filtered through a 0.1 mm membrane was used as microdialysis perfusion buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and blocked in 3% bovine serum albumin (BSA; Millipore) in PBS for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: Indole-3-acetic acid sodium salt (Sigma I5148-2G) was dissolved in water to 500 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 0.2 mM 3-isobutyl-1-methyl-xanthine (Sigma) to prevent resumption of meiosis ...
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bioRxiv - Molecular Biology 2020Quote: ... (3) mouse anti-single stranded DNA (Millipore, 1/100) for 45 min at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... expressed in Rosetta (DE3) plyS cells (Novagen #70956-3) in LB media at 37°C until reaching an OD600of ~0.6 ...
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Proteasome granular localization is regulated through mitochondrial respiration and kinase signalingbioRxiv - Cell Biology 2022Quote: ... and 2-[2-(3-chlorophenyl)hydrazinylidene]-propanedinitrile (CCCP) (Sigma), were used at final concentrations of 0.5 μM ...
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bioRxiv - Microbiology 2020Quote: ... To prepare agarose pad 3% low melting agarose (Sigma) were poured on these slides and left for solidification until further use ...