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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for Recombinant Human MSLN Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Total carbonylated proteins were quantified using OxyBlot Protein Oxidation Detection Kit (Millipore, Billerica, MA) following manufacturer’s instructions using 15 μg of total protein detected with rabbit anti-DNP antibodies (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein precipitation was carried out using ProteoExtract Protein Precipitation Kit (EMD Millipore, 539180-1KIT) according to the manufacturer’s protocol and submitted for MS analysis to the Taplin Biological Mass Spectrometry Facility.
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... Urocortin (rat) protein (Millipore Sigma, ~2μl of 40μM in 1x PBS + 0.1% acetic acid ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Biochemistry 2020Quote: ... Protein A/G beads (Millipore) were then added for overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein G affinity beads (Sigma) were added to the lysate (for anti-D-cys immunoprecipitation only ...
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bioRxiv - Microbiology 2021Quote: ... 2x protein inhibitor complex (Sigma) in PBS was added and protein levels were normalized by Bradford assay ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0 Protein Detection System (Millipore). Membranes were imaged using a chemiluminescent SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cancer Biology 2021Quote: Protein A Dyna-beads (Millipore) were used for immunoprecipitation ...
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bioRxiv - Physiology 2022Quote: ... Protein G Agarose beads (Millipore) and AMPK-IP buffer (50 mmol l-1 NaCl ...
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bioRxiv - Biophysics 2022Quote: ... 1x Protein Inhibitor Cocktail (Sigma), 1 mM PMSF (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: BCA protein assay (Sigma-Aldrich) was performed to measure protein concentrations ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 mM Cas9 protein (Sigma), and 300 mM KCl ...
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bioRxiv - Plant Biology 2019Quote: ... Myelin basic protein (Sigma-Aldrich) was used as an artificial substrate ...
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bioRxiv - Cancer Biology 2020Quote: ... protein G agarose (60µL, Millipore) was incubated with anti-phosphotyrosine antibodies (12µg 4G10 (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A Sepharose beads (Sigma) were then added and the samples were incubated at 4°C for another hour with rotation ...
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bioRxiv - Plant Biology 2021Quote: ... Protein A-agarose beads (Millipore) and an antibody against H3K9ac (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... or S-protein beads (Millipore) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G sepharose beads (Sigma) were then placed in the mixture for 4 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G agarose beads (Sigma) were activated by immersing in water and incubated in IP buffer (300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Protein G Magnetic beads (Millipore) were used for pull-down of PPARG-chromatin complex and analysed using PCR after reversal of cross-linking.
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bioRxiv - Immunology 2022Quote: ... and either Ovalbumin protein (Sigma) or mMOGtag protein.
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Myelin Basic Protein (MBP; Millipore) was used as a positive control ...
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bioRxiv - Neuroscience 2022Quote: ... 2.0 Protein Detection System (Millipore). The following antibodies were used ...
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bioRxiv - Physiology 2022Quote: Magnetic protein G beads (Sigma) were used per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Protein Detection System (EMD Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... or Sepharose protein G (Sigma) were added and further incubated with shaking for 3 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Protein analytical standard solution (Sigma) was used for plotting the standard chart for Stoke’s radius determination.
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bioRxiv - Developmental Biology 2023Quote: ... Heterochromatin protein (Hp1) (MAB3866, Millipore), or Dnmt1 (SantaCruz ...
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bioRxiv - Immunology 2023Quote: ... Protein was trypsin (Sigma Aldrich) digested at 37°C for 18 hours ...
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bioRxiv - Cell Biology 2023Quote: ... with protein inhibitor cocktail (Sigma) and subsequently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... Protein was trypsin (Sigma Aldrich) digested at 37°C for 18 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... human insulin to 10µg/mL (Sigma Aldrich, St. Louis, MO), human epidermal growth factor to 20ng/mL (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 0.005 mg/ml human plasma fibronectin (EMD Millipore) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 ug/ml human insulin (Sigma-Aldrich, Gillingham, UK). D6 ...
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bioRxiv - Cell Biology 2020Quote: ... fibrinogen (human plasma derived) and WKYMVm were from Sigma Aldrich. Recombinant fNLFNYK ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with human rhinovirus 3C protease (8 units/ml; Novagen), and incubated without agitation (4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Human Dermal Fibroblasts were purchased from Sigma (cat # 106-05A). A vial of cells was thawed ...
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bioRxiv - Immunology 2021Quote: ... and human interferon-a 2B (15 ng/mL, Sigma-Aldrich). All cell counts were done manually using a hemocytometer and trypan blue exclusion.
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bioRxiv - Molecular Biology 2021Quote: ... human IPMK cDNA was subcloned into pGEX4T plasmid (Sigma Aldrich), expressed in Escherichia coli ...