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Citations for Millipore Sigma :
2201 - 2250 of 2557 citations for NTR1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated ex vivo for 4h at 37°C with 0.5 μg/ml of SIINFEKL peptide and 10 μg/ml of Brefeldin A (Sigma-Aldrich) in complete RPMI ...
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bioRxiv - Immunology 2021Quote: ... mice were intraperitoneally injected with 100 µg Ag85b280-294 (FQDAYNAAGGHNAVF) or 2W1S (EAWGALANWAVDSA) peptide in 100 µl of a 1:1 emulsion with Complete Freund’s adjuvant (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... WT and Bhlhe40−/− mixed BM chimeras were generated as described above and intraperitoneally injected with 100 µg Ag85b280-294 peptide in 100 µl of a 1:1 emulsion with Complete Freund’s adjuvant (Sigma-Aldrich). On day 11 post immunization ...
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bioRxiv - Immunology 2021Quote: Splenocytes from mice immunized with Lm-Ova 6 wks prior were co-incubated with SIINFEKL peptide (108M) with Golgi Plug/Stop and i) with or without cycloheximide (Translation inhibitor, 10µg/mL, Sigma-Aldrich) or Actinomycin D (Transcription inhibitor ...
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bioRxiv - Genetics 2019Quote: ... The beads were washed 3x with wash buffer and proteins were eluted with FLAG peptide (200 mg/ml, Sigma-Aldrich) in TBS ...
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bioRxiv - Immunology 2022Quote: ... 1 µg/mL SIINFEKL peptide in 200 µL IMDM media with 10% heat-inactivated FBS and 0.05 mM betamercaptoethanol (Sigma-Aldrich) were added to each sample and incubated for a total of 6 h at 37 °C with 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... rPAI-1 was further purified by binding to anti-FLAG agarose beads and elution with 3X FLAG peptide (Millipore Sigma). rPAI-1 was quantified spectrophotometrically (ε280nm = 43,000 M−1 cm−1) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.05% [w/v] digitonin [Sigma: D141], 10% [w/v] glycerol, and 0.4 mg/mL, and 1x FLAG-peptide [Sigma: F3290]) (30 min ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were acidified to 0.67% trifluoroacetic acid (TFA) and then desalted using C18ZipTip (Millipore cat no. ZTC18 5096). Dried peptides were frozen ...
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bioRxiv - Neuroscience 2022Quote: ... LMAN and RA were visualized by immunostaining for calcitonin gene related peptide (Sigma, RRID: AB_259091, 1:5000 to 1:10000) (Bottjer et al. ...
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bioRxiv - Microbiology 2022Quote: ... Samples were eluted in 45 µl of Elution Buffer (100 µg/mL FLAG peptide in Final Wash Buffer; Millipore Sigma) for 25 minutes at room temperature with continuous gentle agitation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (f-MLP) (F3506) and prostaglandin E1 (PGE1) were all purchased from Sigma Aldrich. The purinergic receptor agonist ...
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bioRxiv - Neuroscience 2022Quote: ... peptides were acidified with 2 μl of 1% TFA then desalted using C18 ZipTips® (Millipore, cat no. Z720070-96EA).
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bioRxiv - Molecular Biology 2022Quote: ... yejASm lacking the fragment encoding the first 30 amino acids (signal peptide) was PCR-amplified from Sm1021 genomic DNA and cloned into a pET29b(+) vector (Novagen) generating a C-terminal 6His-tag ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads-antibody-protein mix was incubated overnight at 4°C and bounds protein were eluted with 35 μg HA peptides diluted in PBS (Sigma); then Laemmli buffer was added and the eluate heated at 95°C 2 min.
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bioRxiv - Molecular Biology 2022Quote: ... Elutions were performed by incubating the beads overnight at 4° C with 800 elution buffer and 200 ul of 5mg/ml flag peptide (Sigma). The buffer exchange (into elution buffer ...
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bioRxiv - Systems Biology 2023Quote: ... The solution containing the resuspended peptides were brought to a pH 8.5 and a final concentration of 100 mM HEPES (Sigma H3375) prior to labeling ...
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bioRxiv - Immunology 2023Quote: ... followed by digestion to peptides using 1 µg trypsin at 37°C for 18 hr and the digestion was stopped using 20% TFA (Sigma) to pH 2 to 3 ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered peptide solutions were dried by vacuum centrifugation and desalted and cleaned using a Ziptip (Millipore, Corp., Bedford, MA).
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bioRxiv - Immunology 2024Quote: EAE was induced by subcutaneous immunization with 200 μg MOG35–55 peptide (Peptron, Daejeon, Korea) emulsified in complete Freund’s adjuvant (Sigma-Aldrich), followed by intravenous injection of 200 ng pertussis toxin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Samples were eluted in 45 μl of Elution Buffer (100 μg/mL FLAG peptide in Final Wash Buffer; Millipore Sigma) for 25 min at room temperature with continuous gentle agitation ...
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bioRxiv - Cell Biology 2023Quote: ... and kinetochore particles were eluted by gentle agitation of beads in elution buffer (buffer H + 0.5 mg/ml 3FLAG Peptide [Sigma-Aldrich]) for 30 min at room temperature.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
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bioRxiv - Biophysics 2023Quote: ... 5% CO2 with serial dilutions of G51 peptide or 1% DMSO solution in full culture medium and then stimulated with concanavalin A (ConA, Sigma) in a final concentration 2.5 μg/ml for 5 days ...
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bioRxiv - Molecular Biology 2023Quote: ... The beads were washed three times in IP buffer and eluted twice in 20 µl IP buffer with 100 ng/µl FLAG-peptide (Sigma) for 15 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: All peptides used in this study probes were designed as 11-mers bearing biotinylated PEG2 linker in the N terminus and an amide group in the C terminus and were synthesized using Fmoc-solid-phase peptide chemistry with Novasyn TGR resin (Merck Millipore) as described in the Novabiochem Innovations 01/11 ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Flag M2 beads were washed five times with 1 ml buffer A and then eluted with buffer A supplemented with 3xFlag peptide (Sigma). Unphosphorylated ACKR3 ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were recovered by centrifugation followed by two additional washes with 50mM ammonium bicarbonate and 0.5M NaCl (Sigma-Aldrich, Swisserland). The two filtrates were combined ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 content in the supernatant of T0 and T20 samples was assessed using a Glucagon-Like Peptide-1 (GLP-1) Total ELISA KIT (96-Well Plate Cat. # EZGLP1T-36K, EZGLP1T-36BK, Millipore).
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bioRxiv - Microbiology 2023Quote: ... The resin was washed and incubated with 50 U of TEV protease and 100 µg/mL FLAG peptide (Sigma-aldrich) for 2 h at 18°C ...
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bioRxiv - Developmental Biology 2023Quote: ... A final wash in TBS (20mM Tris HCl, 150mM NaCl, pH 7.6) was done before eluting with FLAG peptide (Sigma, F3290). Elution was done by adding 5x volumes of packed bead volume of FLAG peptide in TBS at 150ng/µl ...
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 10 column volumes of FLAG buffer and proteins were eluted as 1 ml fractions with 100 μg/ml FLAG peptide (Sigma). 5 mM DTT was added to each fraction and peak fractions as determined from Coomassie blue gel electrophoresis were pooled and concentrated in Vivaspin 15-max speed filter (MWCO 30kD ...
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bioRxiv - Immunology 2023Quote: ... for 2h at 4°C and washed four times and precipitated protein were competitively eluted with HA-peptide (100ng/ml; I2149, Sigma).
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bioRxiv - Immunology 2023Quote: ... These were then spiked with synthetic heavy labelled analogues of the peptide targets and a retention time standard (MSRT1, Sigma) for scheduled SRM ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 100 µL of elution buffer supplemented with 200 µg/mL 3× Flag-peptide (Sigma-Aldrich, #F4799) and stored at -80◦C until usage ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purified FH-Ago2 bound with miRNA (most population bound with miR-122 due to its overexpression) was eluted from anti-FLAG beads by competing with 3X FLAG peptide (Sigma) as per the manufacturer’s protocol in a purification buffer (30mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... A binary gradient was used for peptide elution, where mobile phase A consisted of water (Tedia, Fairfield, Ohio, USA) with 0.1% formic acid (Sigma-Aldrich), and mobile phase B consisted of acetonitrile (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... we cleaved the peptides from beads with standard TFA cleavage cocktail (comprising 95% TFA, 2.5% water and 2.5% Triisopropylsilane (Sigma, Cat #233781) for 2-4 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Reh1-bound ribosomes were eluted in 100 ul of IP buffer supplemented with 0.1% Igepal and 150 ug/ml 3xFLAG peptide (Sigma Milipore) for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... beads were washed with IP buffer thrice and the bead bound FLAG-HA-STX5/truncated STX5 was eluted using 3x FLAG peptide (Sigma) as per the manufacturer’s instructions in a purification buffer (30 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... Samples were then washed with lysis buffer containing 0.1% DDM and incubated with lysis buffer containing 1% DDM and 3×Flag peptide (100 µg/ml, Sigma #F4799) overnight for elution ...
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bioRxiv - Cell Biology 2023Quote: ... were fused with mNeongreen at their C-terminus with a flexible peptide linker and cloned into pET28a vector (Millipore; 69864). Protein expression in Escherichia coli BL21(DE3 ...
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bioRxiv - Microbiology 2024Quote: ... The protein was finally eluted with 50 μL of 300 ng/μL 3x FLAG peptide (Sigma-Aldrich, St. Louis, MO) for 45 minutes at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... NEDD4-2 HECT domain was subsequently isolated from the supernatant with anti-FLAG antibody (M2) affinity chromatography and eluted with 100 μg/mL FLAG peptide (Sigma Millipore) in 50 mM TrisHCl ...
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bioRxiv - Immunology 2024Quote: ... of each of the 32 CC strains were immunized subcutaneously with 200 µg MOG35-55 (New England Peptide) emulsified in CFA (Sigma), supplemented with additional 4 mg/ml heat-killed Mycobacterium tuberculosis (Difco Laboratories) ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were pulsed overnight at 37°C with 100 μM of individual peptides in serum-free medium (TexMacs) supplemented with 100 ng/µL of human β2-microglobulin (Sigma), followed by the indicated chase kinetics performed in the presence of Brefeldin A (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were then washed four times with cold lysis buffer and immune complexes were eluted with 75 μL of 3XFLAG peptide (Sigma) (200 mg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were digested by using trypsin at 10 ng/µL at 37°C overnight and peptides were harvested by with 50% ACN/0.1% FA (formic acid, Sigma #06473), lyophilized and resuspended with 0.1% FA ...
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bioRxiv - Genetics 2024Quote: ... cOmplete proteinase inhibitor) to remove nonspecific-binding proteins and then HA-tagged proteins were eluted with HA peptides (Sigma-Aldrich). WT samples (not HA tagged ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...