Labshake search
Citations for Millipore Sigma :
2201 - 2250 of 7695 citations for NT proBNP Protein Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF Immobilon-FL membranes (Merck Millipore) using the Power Blotter Semi-dry Transfer System (Thermo Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... Proteins were blotted onto a PVDF membrane (Merck Millipore, IPVH00010) or nitrocellulose membrane (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were reduced by incubation with 5 mM dithiothreitol (Sigma) for 45 min at 60°C ...
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were blotted to a PVDF membrane (0.45 μm, Millipore) and incubated in a rabbit polyclonal antibody against STOP1 (ABclonal ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto Immobilion-FL PVDF membranes (Millipore IPFL00010) at 100V for 60 min for acute astrocytes ...
-
bioRxiv - Immunology 2024Quote: ... Proteins were then transferred onto 0.45 μm PVDF membranes (Millipore) for wet transfer using Towbin transfer buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
-
bioRxiv - Bioengineering 2023Quote: ... loaded to a polyacrylamide gel (30 μg of total protein/lane) and after gel electrophoresis and protein transfer to polyvinylidene difluoride (PVDF) membranes (Millipore, cat. no. IPVH00010, Burlington, MA). The membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
-
bioRxiv - Molecular Biology 2022Quote: ... 167 mM NaCl) and precleared for 2 h with 250 μL of previously blocked 50% protein-A and protein-G beads (Sigma P-7786 and P-3296 respectively). Blocking was achieved by incubating the beads with 0.5 mg/ml of Ultrapure BSA and 0.2 mg/mL of salmon sperm DNA for 4 h at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... For p65 IP the lysates were then incubated with 2 μg of antibody CST ref #6956 or CST ref# 8242) at 4 °C for 2 hrs before adding 20 μL of slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose) for 20 min ...
-
bioRxiv - Plant Biology 2019Quote: ... Expressed proteins were detected by immunoblotting with anti-FLAG (Sigma, #F1804), anti-cMyc (Fujifilm Wako ...
-
bioRxiv - Cancer Biology 2021Quote: ... Flag-protein complexes were eluted using Flag peptide (Millipore, Cat # F3290) at 150 µg/ml by incubating the beads at room temperature for 30 minutes ...
-
bioRxiv - Biophysics 2019Quote: ... Proteins were transferred onto a Immobilon-P polyvinylidene difluoride membrane (Millipore) using a Bio-Rad Criterion Blotter ...
-
bioRxiv - Biophysics 2020Quote: ... FN3 proteins were expressed in Rosetta DE3 competent cells (EMD Millipore), lysed by sonication ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto an Immobilon-P membrane (PVDF, EMD Millipore) using a Bio-Rad system with a 10% Tris-Glycine and 10% methanol transfer buffer diluted in distilled and deionized water ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were resuspended in 100 mM TEAB buffer (triethylammonium bicarbonate, Sigma) and then digested by trypsin (Pierce MS grade ...
-
bioRxiv - Cancer Biology 2021Quote: Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Darmstadt, Germany) using a BioRad transfer machine after being run through 10% SDS-polyacrylamide (SDS-PAGE ...
-
bioRxiv - Cancer Biology 2021Quote: ... Resolved protein was transferred to PVDF membranes (Millipore, Billerica, MA, USA) using a Semi-Dry Transfer Cell (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2021Quote: ... proteins were detected using either Luminata Crescendo Western HRP Substrate (Millipore) or ECL Western Blotting Analysis System (GE Healthcare ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were then transferred to an Immobilon-P transfer membrane (Millipore) using a Trans-Blot Turbo Transfer system (BIO-RAD) ...
-
bioRxiv - Cell Biology 2019Quote: ... and included protein phosphatase inhibitor cocktails I and III (Sigma-Aldrich) in cell extracts ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration in the supernatant was measured using Bradford Reagent (Sigma), and equal amounts of protein were resolved by SDS-PAGE.
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Burlington, MA, USA) and blotted with anti-phospho-Akt (Ser473 ...
-
bioRxiv - Genetics 2021Quote: ... The expressed proteins were partially purified on Q sepharose (Sigma q1126) and the fractions containing the protein of interest were collected ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to 0.45 μm Immobilon-FL PVDF membranes (Millipore), and membranes were blocked in either 5 % BSA or Odyssey Blocking buffer (LI-COR Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to PVDF-FL transfer membrane (Merck Millipore) at 40V for 90 min at 4 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... proteins were reduced with 1.5 µmol of DTT (Sigma-Aldrich, Canada) for 20 minutes at 70 degrees Celsius ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cells where then lysed with BugBuster protein extraction reagent (Novagen) for 30 min at room temperature ...
-
bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-surfactant associated protein C (SFTPC, 1:1000, Millipore, AB3786), goat anti-podoplanin (PDPN ...
-
bioRxiv - Immunology 2020Quote: A commercial protein-A affinity chromatography called PURE-1A (Sigma-Aldrich.) was used to purify anti-anti-SpA antibodies (Ab-3 ...
-
bioRxiv - Developmental Biology 2020Quote: ... and protein concentration was determined using the Direct Detect spectrometer (Millipore). 20 µg of protein per sample was denatured at 70°C for 10 mins in 1× SDS Loading Dye (100 mM Tris pH 6.8 ...
-
bioRxiv - Developmental Biology 2020Quote: ... the proteins were transferred onto a PVDF membrane (EMD Millipore, IPVH00010). The membranes were blocked with StartingBlockTM T20 (TBS ...
-
bioRxiv - Immunology 2022Quote: Protein lysate was extracted using CelLytic B Cell Lysis Reagent (Sigma) following the manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2022Quote: ... protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred by wet transfer to a PVDF membrane (Millipore) at 4°C overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
-
bioRxiv - Molecular Biology 2021Quote: ... 40 µl of pre-washed protein A beads per sample (Sigma, equilibrated to lysis buffer as mentioned in the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to Immobilon FL (EMD Millipore Corp IPFL00010) membrane in transfer buffer containing 10% methanol and 0.2% SDS ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with primary antibodies to FLAG M2 (Sigma, A8592), HA 3F10 (Roche ...
-
bioRxiv - Neuroscience 2021Quote: ... and house-keeping protein GAPDH (1:500; Mouse; Sigma-Aldrich G8795) or β-actin (1:100 ...
-
bioRxiv - Cell Biology 2022Quote: ... was used to transfer proteins to PVDF Immobilon membranes (Merck Millipore). Blocking and antibody incubation steps were performed in EveryBlot Blocking Buffer (BioRad) ...
-
bioRxiv - Genomics 2020Quote: ... Eluted protein was concentrated by centrifugal ultrafiltration (Millipore Amicon Ultra, 10K), and loaded onto a pre-equilibrated HiLoad Superdex 75 16/60 column (GE Healthcare ...
-
bioRxiv - Systems Biology 2019Quote: ... The 1 mg/mL pure bovine glutamate dehydrogenase (GDH) protein (Sigma) was reacted with 1 mM DSSO in 50 mM HEPES buffer ...
-
bioRxiv - Genetics 2019Quote: ... Proteins were detected with an enhanced chemi-luminescence system (Millipore Luminata) and visualized using an Amersham Imager 600 (GE Healthcare).
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred on Immobilon-FL membrane (Millipore, prod. number IPFL00010), blocked for 1 hr with blocking buffer (LiCOR prod ...
-
bioRxiv - Epidemiology 2019Quote: ... The β subunit protein from cholera toxin was purchased from Sigma Chemical ...
-
bioRxiv - Biochemistry 2020Quote: ... Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore) and analyzed as described above.
-
bioRxiv - Molecular Biology 2019Quote: ... After dialysis protein was concentrated using 10kDa MWCO Amicon filter (Millipore). In vitro SUMOylation assay was performed using SUMO2 Conjugation Kit (BostonBiochem ...
-
bioRxiv - Immunology 2019Quote: ... Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore) visualized on Hyperfilm™ (GE Healthcare).
-
bioRxiv - Biochemistry 2019Quote: ... protein spots were stained with Direct Blue (Sigma-Aldrich, MO, USA). Membrane spots were excised and washed in separate wells in a flat bottom polypropylene 96-well plate ...