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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rabbit anti-zinc transporter 3 (ZnT3 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell proteins were isolated by the SDS-PAGE blotted on PVDF membranes (Millipore). And then blocked with 5% fat-free dry ilk at RT for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... protein was extracted from SC cells using lysis buffer (CelLyticTM M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... These constructs were used for protein expression in Rosetta (DE3) competent cells (Novagen) cultured in TB as reported previously11,46,47 ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μl of Protein G magnetic beads were used and 10 μg of mouse monoclonal anti-FLAG primary antibody M2 (Sigma) were added before overnight incubation at 4°C with the lysate ...
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bioRxiv - Immunology 2021Quote: ... In the three subsequent boosters the amount of protein used was 25 μg per mouse mixed with incomplete Freund’s adjuvant (Sigma, India). Boosts were given on days 15 and 28 post-priming ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and nsp12 proteins were subsequently detected using the mouse monoclonal anti-DYKDDDDK M2 antibody (Catalogue number F3165, Sigma-Aldrich) diluted 1:1000 in TBST/5% milk ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then pre-cleared using 25 μL of Protein A agarose beads (Pierce) and then incubated with 25 μL of mouse anti-V5 agarose beads (Sigma) and washed with RIPA buffer ...
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bioRxiv - Plant Biology 2022Quote: ... A 1–month–old ICR mouse (SLAC laboratory animal) was injected with 50 μg GST–CAR9 protein emulsified with Complete Freund’s adjuvant (F5881, Sigma–Aldrich). Two weeks later ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cell Biology 2020Quote: ... were washed with 100 μl worm protein lysis buffer and incubated with monoclonal M2 mouse anti-Flag antibody (Sigma, F3165) or monoclonal rabbit anti-c-Myc antibody (Sigma ...
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bioRxiv - Genetics 2021Quote: ... were superovulated by serial Pregnant Mare Serum Gonadotropin (5 IU per mouse, Prospec Protein Specialists) and human chorionic gonadotropin (5 IU, Millipore) injections 46 h apart ...
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bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...
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bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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bioRxiv - Molecular Biology 2022Quote: ... Golgi apparatus was detected with mouse anti-Golgi 58K protein /formiminotransferase cyclodeaminase (FTCD) antibody (G2404, SIGMA-Aldrich, Prague, Czech Republic). The following secondary antibodies were used for detection ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
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bioRxiv - Immunology 2022Quote: ... growth factors) in mouse whole lung protein were quantified using EMD Millipore’s MILLIPLEX Magnetic Bead Panel (Millipore Sigma #MCYTOMAG-70K), including ...
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bioRxiv - Genomics 2019Quote: Mouse ES cells were routinely cultured on cell culture-treated dishes coated with 0.1% gelatin (Sigma #G9391-100G) using the following culture medium ...
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bioRxiv - Neuroscience 2022Quote: ND7/23 cells (a mouse neuroblastoma × rat dorsal root ganglion neuron hybrid cell line; Sigma−Aldrich, ECACC 92090903), were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Genetics 2020Quote: ... The ES cells were plated on mitomycin C treated mouse embryonic fibroblast cells (Millipore Sigma #PMEF-NL-P1) that are G418 resistant ...
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bioRxiv - Cell Biology 2024Quote: ... mES cells were cultured on mitomycin-treated mouse embryonic fibroblasts (feeder cells) plated on 0.1% gelatin (Sigma-Aldrich) in mouse embryonic stem cell medium supplemented with leukemia inhibitory factor (LIF ...
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bioRxiv - Biochemistry 2024Quote: HT-22 cells (Mouse Hippocampal Neuronal cell line) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, D7777) containing 10% fetal bovine serum (CRIPION ...
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bioRxiv - Immunology 2021Quote: ... ELISA plates were coated with the capturing reagent in PBS [OVA (Sigma, A5503) for antigen ELISAs ...
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bioRxiv - Microbiology 2021Quote: ... 5′5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, Taufkirchen, Germany) was added until a colour change was observed ...
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bioRxiv - Neuroscience 2021Quote: Alongside the ELISA a Bicinchoninic acid assay (BCA) assay (#71285-3, Novagen, Millipore) was performed according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Alongside the ELISA a Bicinchoninic acid assay (BCA) assay (#71285-3, Novagen, Millipore) was performed according to manufacturer’s protocol ...
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bioRxiv - Pathology 2019Quote: ... BR) and insulin Elisa assay (cat#EZRMI-13K, Merck Millipore, Billerica, MA, EUA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Overnight fasting serum levels were quantified by ELISA for insulin (#EZRMI-13K; Millipore) and leptin (#90030 ...
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bioRxiv - Immunology 2022Quote: ... SAA and CTX-I were measured using the human SAA ELISA (Sigma-Aldrich) and Serum Crosslaps (CTX-I ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma insulin was measured by enzyme-linked immunosorbent assay (ELISA) (Millipore, Burlington MA).
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bioRxiv - Immunology 2024Quote: ... 96-well ELISA plates were first coated overnight with poly-L-lysine (Sigma), then overnight with DNA from calf thymus (Sigma ...
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bioRxiv - Physiology 2023Quote: ... and plasma insulin (n=5) was analyzed by ELISA (EZRMI-13K, Sigma Aldrich), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Costar 96-well ELISA plates were coated with 10 μg of fibronectin (Sigma) for the fibronectin-binding assay and 10 μg of fibrinogen (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: Cell proliferation was assessed using Cell Counting Kit-8 (CCK-8, Sigma) following manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... cell viability was measured with a cell proliferation kit (MTT, 1146500700, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Percentage cell death was measured using Cell Cytotoxicity Detection kit (LDH; Sigma) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... 4-dinitrophenylhydrazine using protein carbonyl content assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the adduct was examined at 375 nm ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Total protein staining of membrane was done using an R-PROB staining kit from Sigma. Primary antibodies were incubated overnight at 4°C and secondaries for 1 hr at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were purified using Protein G Immunoprecipitation Kit (Cat. No. IP50, Sigma-Aldrich Inc.) and were separated on SDS-PAGE for western blotting using monoclonal antibodies against T18 as described above ...
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bioRxiv - Microbiology 2020Quote: The DRM and DSM fractions119 were separated using the CelLytic MEM Protein Extraction Kit (Sigma). 600 μl of lysis and separation buffer were added to 50 μl of resuspended membrane and incubated at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... The protein bands on the PVDF membranes were observed using an enhanced chemiluminescence kit (Millipore) and quantified using Image J software (NIH ...
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bioRxiv - Physiology 2021Quote: ... The remaining supernatant was precipitated using the Calbiochem Protein Extraction Kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...