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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for Homeobox B2 HOXB2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The membrane was probed overnight at 4°C with blocking buffer containing rabbit anti-Extramacrochaetae antibody and an antibody to β-actin (Sigma-Aldrich). The membrane was then washed four times with 1X PBST for five minutes each ...
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bioRxiv - Immunology 2019Quote: ... an Alexa Fluor™ 568 antibody labeling kit and a Mix-n-Stain™ CF®633 Dye antibody labeling kit (Sigma) were used according to manufacturer’s protocols.
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bioRxiv - Genomics 2020Quote: Approximately 5 × 106 cells from the overnight incubation with the primary/secondary antibody-antibody complex was stringently washed with NET-2 buffer then denatured in a DL-Dithiothreitol (DTT) (Sigma-Aldrich D9779), NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used: DYKDDDDK Tag Antibody (D6W5B rabbit mAb #14793; Cell Signaling Technology; it binds to the same epitope as Sigma’s Anti-FLAG M2 Antibody ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections incubated at room temperature overnight in primary antibody medium or control medium (1:100 primary anti β-catenin antibody from rabbit Sigma C2206 + 1:100 primary anti β-actin antibody from mouse Sigma A5441 + PBS pre-incubation medium ...
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bioRxiv - Neuroscience 2021Quote: ... the slides were incubated at 4°C for 24 hours with the following primary antibodies: (I) rabbit polyclonal anti-ERα antibody (1:2,000; Sigma-Aldrich, 06-935) (II ...
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bioRxiv - Microbiology 2021Quote: ... Total cell lysate was also subjected to immunoblotting with anti-T7e rabbit antibodies and anti-HA rabbit polyclonal antibodies (Sigma Aldrich, H6908) to evaluate the expression levels of Env-T7es and HA-MARCH8 ...
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bioRxiv - Microbiology 2021Quote: ... gondii localized using antibody against the RH strain tachyzoites (67) and ubiquitin was localized using mouse antibody clone FK2 (04-263; EMD Millipore Corporation). Parasites were enumerated per parasitophorous vacuole (PV ...
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bioRxiv - Physiology 2020Quote: ... α-cell/ β-cell ratio was calculated following staining with antibodies against insulin (as above) and glucagon (primary antibody: mouse anti-glucagon 1:2000; Sigma-Aldrich, G2645) (secondary antibody goat anti-mouse Alexa Fluor 488 1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunoprecipitations were performed overnight at 4°C on a rocking wheel using either mouse anti-MAGI1 antibody for endogenous immunoprecipitations or anti-HA antibody and EZview Red anti-FLAG M2 affinity gel (Sigma-Aldrich #F1804) for co-immunoprecipitations ...
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bioRxiv - Cancer Biology 2021Quote: ... To the remaining cell lysate was added anti-H3K4me3 antibody-coated Dynabeads M-280 Sheep anti-Rabbit IgG (5 μg antibody per sample, Millipore, 04-745), followed by rotation at 4°C overnight for immunoprecipitation ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilized cells were incubated with the specified primary antibodies (mouse monoclonal anti-acetylated tubulin T6793, Sigma-Aldrich; and mouse monoclonal anti-FLAG M2 antibody, F1804, Sigma-Aldrich) for 1 h washed with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibody 1:12000 mouse anti-α-Tubulin antibody (SIGMA, Cat. # T5168). Followed by 1h incubation with secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... the primary antibody was discarded and the preparations probed with a secondary antibody (anti-rabbit, conjugated to Fluorescein isothiocyanate, FITC (Sigma-Aldrich, Germany), prepared at a dilution of 1:10000 in PBST for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: For immunocytochemical staining we used the following primary and secondary antibodies: mouse anti-NFL antibody clone DA2 (Merck Millipore, cat. no. MAB1615), mouse anti-NFL antibody clone F-12 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2019Quote: NQO1 and GCLC antibodies were switched during the course of this study to Abcam antibodies when new lots of original antibodies did not produce the same specificity as the older lots (Cell Signaling and Millipore, respectively). NQO1 (Cell Signaling ...
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bioRxiv - Biochemistry 2020Quote: ... Acetylation of cellular Pif1 was detected by immunoprecipitating cell lysate with anti-acetyl lysine antibody (CST 9441) resin and immunoblotting with an anti-FLAG M2 antibody (Millipore Sigma A8592). Specifically ...
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bioRxiv - Neuroscience 2019Quote: ... the membranes were incubated with guinea pig antibody to D1 receptors (1:400; Alomone, Jerusalem, Israel) and rabbit antibody to D2 receptors (1:500; Millipore, Burlington, MA), washed ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 μl of CHAMP buffer containing 4 nM Alexa488-conjugated anti-Flag antibody (Alexa Fluor 488 antibody labeling kit, Thermo Fisher; monoclonal BioM2, Sigma-Aldrich) was injected to wash off unbound RNP and label DNA-bound RNP complex ...
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bioRxiv - Microbiology 2020Quote: Secondary antibodies used are: Goat anti-mouse IgG (H+L) highly cross-adsorbed Alexa fluor plus 647 secondary antibody (Sigma-Aldrich, A32728), Goat anti-mouse IgG (H+L ...
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bioRxiv - Physiology 2019Quote: ... and incubated for 2 h at room temperature with anti-myosin heavy chain I antibody (MHCI; mouse IgM, Developmental Studies Hybridoma Bank: reference #A4.480) and anti-dystrophin antibody (mouse IgG2b, Sigma Aldrich: reference #D8168) in 5% goat serum diluted in PBS (1:1 PBS dilution) ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were washed with TBST for 3 times and incubated for 1 h with a secondary antibody: anti-mouse IgG antibody (HRD) (Sigma-Aldrich, A9044) and anti-rabbit IgG antibody (HRD ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to primary antibody solution containing monoclonal mouse anti-vesicular glutamate transporter 1 (VGlut1) antibody (1:750; Millipore, Burlington, MA) in blocking buffer and incubated overnight at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... FLAG-tag immunoprecipitation was performed using an α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165) and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G ...
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bioRxiv - Neuroscience 2020Quote: ... one was reacted against anti-calbindin antibody raised in mouse (Swant Inc., Marly, Switzerland) and another against anti-NeuN antibody raised in mouse (EMD Millipore, Germany). The remaining series was reacted with either anti-parvalbumin antibody raised in mouse (Swant Inc. ...
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bioRxiv - Genetics 2020Quote: ... using 1:1000 rabbit anti-Bestrophin1 antibody (LAgen Laboratories; Cat# 016-Best1-01) and 1:1000 mouse anti-Actin antibody (Millipore; Cat# MAB1501) as primary antibodies.
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated in PBS-T-milk for 1 h using 1:10000 dilutions of anti-rabbit or anti-mouse HRP-linked secondary antibodies (A0545 or A9044 Sigma antibodies, respectively) as required ...
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bioRxiv - Biophysics 2022Quote: ... Solutions of primary antibodies were prepared in blocking solution using the following dilutions: mouse antibody against SRRM2 (1:2000, Sigma Aldrich, #S4045), mouse antibody against SRSF1 (1:250 ...
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bioRxiv - Biochemistry 2022Quote: ... neutralizing monoclonal antibodies against human catalase (clone CAT-505, mouse, IgG1) and control antibodies directed against laminin were obtained from Sigma (Schnelldorf, Germany).
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bioRxiv - Neuroscience 2022Quote: ... and 1 hour in secondary antibody (1:500 Alexa Fluor 568 donkey anti mouse antibody, Cat: A10037, Lot: 1917938)) with DAPI (1:1,000 Millipore Sigma, D9542-1MG). Tissue was washed 5 times for 5 minutes each in 1x PBS between each treatment and mounted on Fisherbrand Superfrost Plus slides ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with fluorophore-conjugated primary antibodies against NeuN and Fos: phycoerythrin (PE)–labeled anti-NeuN antibody (1:500; catalog no. FCMAB317PE, Millipore. RRID: AB_10807694) and Alexa 647–labeled anti–phospho-Fos antibody (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with CR3022 anti-S antibody (1 ug/mL)128 overnight followed by anti-human IgG-HRP-conjugated goat secondary antibody (Sigma, 1:500) for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... The primary and secondary antibodies used for GABA-IHC were a rabbit anti-GABA antibody (1:200; Sigma Aldrich, Burlington, MA; RRID:AB_477652) and an Alexa Fluor® 647 AffiniPure™ Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were purchased: rabbit anti-PITPNB polyclonal antibody (pAb) and sheep anti-TGN46 pAb from Sigma-Aldrich (St. Louis, MO); rabbit anti-β-tubulin pAb from cell signaling (Danvers ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in the Antibody Diluent Reagent from the kit [mouse anti-c-myc (clone 9E10; 1:200, Sigma-Aldrich #11667149001) and rabbit anti-HA (1:200 ...
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bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with primary antibody (15 µg/mL in 3% BSA in PBS): rabbit anti-KCNN4 antibody (IgG, clone HPA053841, Sigma Aldrich®) or control rabbit antibody (IgG ...
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bioRxiv - Neuroscience 2023Quote: ... 0.04% NaN3 containing different antibodies: 1:1000 PS38 Neurophysin-I (gift from Harold Gainer, antibody now commercially available through Sigma-Aldrich MABN844), 1:1000 anti-glutamine synthetase (monoclonal mouse ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were probed overnight at 4°C with anti-HA antibody (MBL, M180-3) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Sigma-Aldrich, G8795) or with anti-dystrophin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then blocked in 3% BSA before adding primary antibody mouse monoclonal anti-IAV nucleoprotein A1 and A3 blend antibody (EMD Millipore, MAB8251). The primary antibody was followed by a secondary anti-mouse Alexa-488 (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked 1 hr in antibody buffer at RT and incubated depending on the experiment with following primary antibodies: Anti- Acetylated Tubulin (1:1000, Sigma-Aldrich, #T6793); anti ZO-1 (1:200 ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... 1% goat serum, and a 1:1000 dilution of either the AH36 antibody (StressMarq Sciences, #SMC-601) or T22 antibody (Millipore Sigma, #ABN454) in 1xTBS ...
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bioRxiv - Neuroscience 2024Quote: ... followed by primary antibodies 24-48 hours at 4°C with primary antibodies against BRN3A (1:50 dilution; Millipore Cat# MAB1585, RRID:AB_94166), RBPMS (1:200 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Neuroscience 2024Quote: ... and incubation at 4°C overnight with a mixture of the following primary antibodies: anti-ChAT antibodies (1:50, AB144P; Millipore, UK; RRID:AB_2313845); anti-GFAP antibodies (1:2000 ...