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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
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bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...
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bioRxiv - Microbiology 2023Quote: ... 1000 µg of protein extracts were cleared with agarose protein A beads (Millipore) at 4 ℃ for 1.5 h on a rocking platform ...
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bioRxiv - Plant Biology 2023Quote: ... the protein extract was incubated with 30 μL Protein A Agarose beads (Millipore), 10 μL anti-SIZ1 antibody with rotation at 4°C for 4 h.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified using a Protein A agarose gravity column (Millipore Sigma) followed by SEC using a S200 column.
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ku80 (CST, #2180, 1:400), anti-human nuclear antigen (Novus NBP2-34342, 1:200) and anti-human-specific Albumin (Sigma, A3293, 1:200). Species-specific secondary antibodies conjugated with Alexa Fluor 555 ...
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bioRxiv - Cell Biology 2021Quote: ... Rats were fed a high fat and high carbohydrate diet (HFD) for one month after which streptozotocin (Sigma-Aldrich, St. Louis, MO, USA, 40 mg/kg) dissolved in 10 mmol/L citrate buffer (pH 4.5 ...
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bioRxiv - Immunology 2021Quote: Mouse groups (n=4) were injected with 100μl of vehicle (PBS, or No Drug) or immunosuppressants: cyclophosphamide monohydrate (CYC, Sigma Aldrich C0768, 60mg/kg every other day), leflunomide (LEF ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: High-binding 96-well plates (Cat# CLS3361; Millipore Sigma, Burlington, MA) were either coated directly with 500 ng/well of recombinant DENV2 16681 E protein (Cat# DENV2-ENV-500 ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 5% FBS and HEK293T-LentiX in high-glucose DMEM (Sigma) containing 10% FBS in a 5% CO2 incubator at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... Dulbecco’s Modified Eagle’s Medium - high glucose (DMEM) (#RNBG3787, Sigma, Buchs, Switzerland) supplemented with (10% ...
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bioRxiv - Neuroscience 2019Quote: ... High purity deionized water (ddH2O) was filtered from Millipore (18 OMG). OmniTrace glacial acetic acid and ammonium hydroxide were obtained from EMD Chemicals ...
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A quantitative analysis of the interplay of environment, neighborhood and cell state in 3D spheroidsbioRxiv - Systems Biology 2020Quote: ... University of Manchester) were grown in high-glucose DMEM (D5671, Sigma). HT29 (ATCC HTB-38 ...
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bioRxiv - Biochemistry 2020Quote: ... ampholyte high resolution pH 7–9 were purchased from Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2020Quote: ... BHK21-hACE21 cells were maintained in high glucose DMEM (SIGMA-ALDRICH) supplemented with 10% FBS (GIBCO) ...
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bioRxiv - Cell Biology 2021Quote: ... A single high dose (200 mg/Kg) of streptozotocin (Sigma, #S0130) was administered via intraperitoneal injection two days before transplant to induce hyperglycemia ...
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bioRxiv - Cell Biology 2021Quote: 3T3-L1 (RRID_0123) cells were maintained in high glucose DMEM (SIGMA) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Systems Biology 2022Quote: ... high glucose Dulbecco’s Modified Eagle Media (DMEM, D0822/D5671, Sigma-Aldrich) containing 10% FBS and 1% PS was used as medium for one week ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-OC against high molecular weight Aβ (Merck Millipore, Sweden), mouse anti-6E10 for human Aβ/APP (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... The agarose mixture consisted of 0.4g high EEO Agarose (Sigma-Aldrich) 0.42g Certified Low-Melt Agarose (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... The mixture consisted of 0.4 g high EEO Agarose (Sigma-Aldrich), 0.42 g Certified Low-Melt Agarose (Bio Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Taste chemicals were of high purity (Sigma-Aldrich, St. Louis, MO) and dissolved in purified water.
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bioRxiv - Physiology 2022Quote: ... high glucose supplemented with 1% Extracellular Matrix (Sigma-Aldrich, USA/UK), 2 µg/mL fibronectin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... cynomolgus and porcine fibroblasts were cultured in high-glucose DMEM (Sigma) supplemented with 15% FBS ...
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bioRxiv - Microbiology 2019Quote: ... cells were maintained in Dulbecco’s modified high glucose medium (DMEM, Sigma) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... J2-3T3 cells were cultured in high-glucose DMEM (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: ... at 100 cells/mm2 in high glucose growth medium (D5671, Sigma), containing 10% foetal calf serum and 2% glutamine ...
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bioRxiv - Synthetic Biology 2020Quote: PCR reactions were performed with Expand high Fidelity system (Sigma-Aldrich), Phusion DNA Polymerase (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... A375ma2 (ATCC) melanoma cells were maintained in high glucose DMEM (Sigma), supplemented with 2mM L-glutamine (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were cultured in high-glucose DMEM (Sigma-Aldrich; Cat# D5671) supplemented with 20% FBS ...
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bioRxiv - Immunology 2021Quote: ... The high mannose glycoproteins subsequently were treated with endoglycosidase F1 (Millipore) to obtain homogeneously deglycosylated RBD.
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bioRxiv - Developmental Biology 2021Quote: ... The cells were cultured in DMEM-high glucose media (D5796; Sigma) with 10% BCS (Sigma-Aldrich) ...