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Citations for Millipore Sigma :
2201 - 2250 of 2842 citations for 6 METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... a total of 0.4 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) and cells were fixed 4 days later ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Developmental Biology 2022Quote: ... A few hours later the cells were infected with titer-adjusted volumes of each viral supernatant supplemented with 6 μg/ml (final concentration) of protamine sulfate (Sigma). After two days ...
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bioRxiv - Cell Biology 2022Quote: NOD/SCID mice (6 weeks-old) were treated with an intraperitoneal (ip) injection of 650 mg APAP/kg (Sigma-Aldrich) to induce ALF ...
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bioRxiv - Developmental Biology 2022Quote: ... 2/3 mTeSR1 + 1/3 N2 medium + LSX + PSD Day 6,7: 1/3 mTeSR1 + 2/3 N2 medium + PSD At day 6 cells were detached by Accutase solution (Sigma-Aldrich) and incubated at 37°C for 20 minutes to obtain a single cell suspension ...
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bioRxiv - Cell Biology 2022Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 (mouse ...
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bioRxiv - Cell Biology 2022Quote: ... The following incubations ensued at room temperature: 3×10 min in 0.1 M maleic acid buffer (Sigma-Aldrich; pH 6), 2 h in the dark in 0.5% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... The explants were cultured for 6 days in DMEM with 10% fetal bovine serum and 10 μg/ml ciprofloxacin (17850, Sigma), and then fixed in 4% PFA for 15min at room temperature before being processed for immunolabeling.
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bioRxiv - Immunology 2022Quote: ... The sections were then washed with PBS/0.5% Triton and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Digitally scanned sections were analysed using Image Scope software (Scanscope XT ...
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bioRxiv - Immunology 2022Quote: ... Lysates were assayed for phospho proteins using the Milliplex Cell Signaling kit (NFκB Magnetic Bead Signaling 6-plex, Millipore Sigma) for detection of NFκB pSer536 ...
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bioRxiv - Immunology 2022Quote: ... Progenitors were spinfected in 6-well plates at 23-32°C for 30 minutes at 1000 x g with 4 µg/mL polybrene (Sigma) and 1 µM β-estradiol at 0.25 × 106 cells/mL for single gene knockouts ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 µL of the mix (corresponding to 8.105 cells) was then plated on 6 cm Petri dishes filled with 2 mL of 2% Phytagel (Sigma-Aldrich), following Dubravcic et al (Dubravcic et al. ...
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bioRxiv - Immunology 2022Quote: ... synthesized as previously described),6 a 5 mg/mL stock solution of PBI1 dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich) was prepared ...
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bioRxiv - Plant Biology 2022Quote: ... total glutathione in 0.1 ml of the supernatant was measured by spectrophotometry in a 1 ml mixture containing 6 mM DTNB (Sigma-Aldrich), 3 mM NADPH ...
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bioRxiv - Immunology 2022Quote: Dorsal and ventral side ears of either Mas-TRECK mice or wild type (C57BL/6) mice (WT) were injected intradermally (i.d.) with 10 ng of diphtheria toxin (DT) (Sigma-Aldrich) in 20 µl of PBS for one time ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
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bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... the embryos were arranged in glass Petri dishes (90 × 15 mm) and immersed in 6 mL stationary MS liquid culture medium (Murashige and Skoog, 1962) (Sigma) containing 0.1 g L-1 myo- inositol (Sigma) ...
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: ... Cochlear cultures were treated for 6 h with various reagents (all dissolved in culture medium): 0.1% (v/v) DMSO (276855; Sigma-Aldrich), 0.5 mM BAPTA-AM (A1076 ...
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bioRxiv - Neuroscience 2022Quote: ... The silk solution concentration was adjusted to 6 mg/mL and combined in a 10 cm dish with 400 to 500 µm sodium chloride (Sigma) particles at a ratio of 1:2 (v/w ...
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bioRxiv - Neuroscience 2022Quote: ... mito-tempol[23] (25 μg, Sanbio) and oligodeoxynucleotide (3 μg/μl day 4, 5 and 6 after carrageenan, Sigma-Aldrich), were performed under light isoflurane anesthesia as described.[8a ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 mM NaCl and 6 mM β-mercaptoethanol) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and Protease inhibitor cocktail (Sigma-Aldrich) (20 mg/g of cell pellet) ...
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bioRxiv - Cell Biology 2022Quote: ... Parasitemia was adjusted to 0.2-0.5% and 2% hematocrit and the culture was distributed in triplicates in 6-wells plate with various treatments: 400pM of the mimic (HMI0513-5NMOL, Sigma) or 400pM of the inhibitor (cat# 4464084 ...
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bioRxiv - Bioengineering 2022Quote: ... PAAm matrices were activated twice with 0.5 mg/mL Sulfo-SANPAH for 6 minutes at room temperature (Sigma-Aldrich, 80332) and coated with 5 μg/mL fibronectin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... T cell subsets were rested overnight at 37°C then stimulated for 6 h with phorbol 12-myristate 13-acetate (25 ng/mL; Sigma) and ionomycin (1 mg/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... All samples diluted with buffer to the final concentration of 2 μM Aβ were supplemented with 6 μM ThT (Sigma), with a range of concentrations of the binders per experiment ...
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bioRxiv - Biochemistry 2022Quote: ... The protein samples of the peak fractions were collected and concentrated to 6 mg/ml using a 100 kDa molecular weight cut-off concentrator (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... centrifuged and the cell pellet was lysed using a Lysis Buffer (25 mM Tris pH 7.6, 150 mM NaCl, 1% NP-40, 1% Na-deoxycholate, and 0/1% SDS in water + protease inhibitor cocktail [Sigma] + phosphatase inhibitor cocktail [Sigma-Aldrich] + okadaic acid + sodium fluoride) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with ice-cold PBS and incubated with 0.25 µg/mL biotin hydrazide (66640-86-6, Sigma-Aldrich) in cold PBS for 1 hour on ice ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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bioRxiv - Immunology 2022Quote: ... were administered to C57BL/6 mice by subcutaneous (SC) injections on days 0 and 28 in the presence of Sigma adjuvant system (Sigma). 2 µg of S and S-EABR mRNA-LNP were administered to BALB/c mice by intramuscular (IM ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were plated at a density of 8x10^6 cells per 10 cm dish coated with 60 μg/ml poly-D-lysine (Sigma) and 2.5 μg/ml laminin (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... Disposable PS plastic was readily available as the flat bottom of a 6-well tissue culture plate (TPP®, Sigma). Disposable glass cover slides (22CIR-1 ...
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bioRxiv - Biochemistry 2022Quote: ... cells were mounted in Sigma-Aldrich DuoLink in situ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich), allowed to incubate at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... 3533-005-02) and overlaid with Organoid Media (Supplementary file 1) in 6-well ultra-low adhesion plates (Sigma, CLS3471). H2B:GFP retrovirus (91 ...
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bioRxiv - Immunology 2023Quote: ... Interphase cells were collected and incubated at 37°C in 5% CO2 for 6 hours with 20ng/ml Phorbol 12-Myristate 13-Acetate (PMA, Sigma), 1µg/ml Ionomycon (Sigma ...
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bioRxiv - Genomics 2023Quote: C57BL/6 mouse ESC were purchased from ATCC (SCRC1002) and maintained onto 1% gelatin-coated (Sigma-Aldrich, Cat. No G1393) dishes in DMEM knockout medium (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Monocytes were then isolated with CD14+ microbeads (MACS Miltenyi) and incubated for 6 days in RPMI/10%FBS and 1% penicillin/streptomycin solution (Sigma) supplemented with 50 ng/mL human GM-CSF (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... 0.4 × 106 CXCR3A-transfected CHO cells/ml or 60 000 HMVEC/ml (2ml/well) were seeded in flat bottom 6-well plates (2.0 ml/well; TPP, Sigma-Aldrich) in supplemented Ham’s F-12 growth medium + 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1-1.2x106 cells/ml onto 6-well plates treated with poly-D-lysine (EMD Millipore). Cells were cultured in Neurobasal A medium supplemented with 2% B27 ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Bioengineering 2023Quote: ... 3D collagen matrix systems were stained with 50 µM of 5-(and-6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE, Sigma-Aldrich) at room temperature for 60 minutes and visualized using a confocal laser scanning microscope (cLSM ...
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bioRxiv - Microbiology 2023Quote: ... a dilution series of 107 to 103 cells/ml were plated in 5 μl spots on M9 minimal media plates with 10 mM glucose or 10 mM glucose-6-phosphate (Sigma) and antibiotics ...
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bioRxiv - Neuroscience 2023Quote: ... Mice fasted overnight were gavage fed 6% carmine red dye (300 µl) Sigma-Aldrich) in distilled water containing 0.5% methylcellulose (Sigma-Aldrich) and placed in individual cages with white paper covering cage bottoms ...
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bioRxiv - Neuroscience 2023Quote: ... Adult female SNSiDTR (n=6) and TrkBiDTR (n=19) mice were injected i.p with 40 μg/kg DTX (Sigma, D0564) with a second dose after 72 h ...
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bioRxiv - Microbiology 2023Quote: ... quenched with 25 mM ammonium chloride in PBS for 10 min and nuclei were stained with 4’,6-diamidino-2-phenylindol (DAPI, Sigma, cat ...
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were washed three times with ice-cold PBSx1 and lysed using Maltoside lysis buffer [1% N-dodecyl maltoside (cat no. 69227-93-6 – Sigma) dissolved in PBSx1 with freshly added 0.1 mg/ml PMSF and protease inhibitors] ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 30min at room temperature (10µg/mL, D9542, Sigma-Aldrich). Coverslips were mounted with ProLong Gold Antifade (P36934 ...
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bioRxiv - Genomics 2023Quote: ... or cell type (roughly 5X106 iPSC-NPCs or 1X106 iPSC-neurons on a 6-well plate, enzymatically detached with Accutase (Millipore) and pelleted with centrifugation for 5 mins at 1,000 G) ...