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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for 6 Amino benzooxazole 2 thiol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were recovered for 1 hour in YPD prior to dividing the culture into 5 aliquots of 200 µL and plating on yeast nitrogen base plus amino acids without uracil (Sigma).
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bioRxiv - Molecular Biology 2019Quote: ... These base media were then supplemented with 84 mg/mL 13C615N4 L-arginine plus 146 mg/mL 13C615N2 L-lysine or the corresponding non-labeled amino acids (all from Sigma) to generate either heavy or light media ...
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bioRxiv - Synthetic Biology 2020Quote: ... Promoter and terminator characterization experiments were conducted in synthetic media containing 0.67% (w/v) Yeast Nitrogen Base Without Amino Acids (Sigma-Aldrich), 2% Glucose (Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... The fertilized eggs were transferred to 5 μL droplets of potassium simplex optimization medium with amino acids (KSOMaa) containing 0.00025% polyvinyl alcohol (P8136-250G; Sigma-Aldrich) and 100 μM ethylenediaminetetraacetic acid on a glass-bottomed dish and placed in an incubation chamber (Tokai Hit ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were inoculated into 200 ml MGY liquid media composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626), 0.4 mg/L biotin and 1% glycerol and cultured at 30°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Frozen tissue or cell pellets were boiled for 24 hours at 100°C in 500µL (cell pellets) −1mL (tissue) 6M HCl for amino acid analysis (Sigma 84429). 50µL (tissue ...
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bioRxiv - Microbiology 2021Quote: ... washed twice with PBS and the medium changed to amino acid free DMEM (Genaxxon) supplemented with 100 μg/ml of penicillin-streptomycin mix (PenStrep) (Sigma) and rapamycin 0.35 µM (selleckchem) ...
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bioRxiv - Microbiology 2021Quote: ... and the medium changed to amino acid free DMEM (Genaxxon) supplemented with 100 μg/ml of penicillin-streptomycin mix (PenStrep) (Sigma). The cells were incubated overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Caco-2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Media Preparation Unit, Peter Doherty Institute) supplemented with 1X non-essential amino acids (Sigma-Aldrich), 20 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... the coding region corresponding amino acid 321-475 of dHtt protein was amplified by PCR and cloned into pET28b expression vector (Novagen). To generate anti-CG8134 antibody ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat. M7145) and sodium pyruvate (Gibco cat ...
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bioRxiv - Systems Biology 2022Quote: Defined minimal media contained 6.7 g/L of yeast nitrogen base without amino acids (cat# Y0626, Sigma-Aldrich, MO, USA), 20 g/L of glucose ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Neuroscience 2019Quote: Tau variants (full length protein and a fragment encoding amino acids 256-368) were cloned into the pET19b vector (Novagen) in between the NcoI and BamHI restriction sites ...
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bioRxiv - Genomics 2020Quote: ... embryoid bodies were transferred to ultra-low-attachment 6-well TC plates in neural induction media (DMEM/F12, 1% Glutamax, 1% non-essential amino acids and 10 µg/ml heparin (Sigma). After another 4-5 days ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then grown for ≈2 generations (OD600 ≈0.2) before amino acid starvation was induced by the addition of either 500 μg/ml valine (Sigma Aldrich) or 400 μg/ml serine hydroxamate (Sigma Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... Then 1 × 107 cells were washed with sterile water and transferred to nitrogen starvation conditions [6.7 g/L YNB without amino acids and without ammonium sulfate (Sigma-Aldrich) and 20 g/l glucose] and grown at 30 °C and 200 rpm ...
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bioRxiv - Biochemistry 2020Quote: Rabbit polyclonal anti-TcHTE antibodies were obtained using the p1TcHTE peptide (amino acid 120 to 171) expressed as a TRX.HIS-fusion protein in the vector pET-32a vector (Novagen®), following same strategy we described previously (23) ...
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bioRxiv - Microbiology 2021Quote: ... cell cultures were diluted in 5 ml of M9 with 0.4% glucose and 0.08% RPMI 1640 amino acids (Sigma-Aldrich R7131), and incubated in 37°C (strain EL2504) ...
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bioRxiv - Immunology 2021Quote: ... HLA typed HLA-A*02:01+ PBMCs from COVID-19 recovered individuals were incubated with SARS-CoV-2 peptide pools (2µM / peptide) and cultured for 10-14 days in RPMI-1640 supplemented with 1x Non-essential amino acids (NEAA; Sigma), 5 mM HEPES (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... and human choriocarcinoma/trophoblastic cancer (JEG-3) cells were grown in DMEM supplemented with 10% FBS and MEM Non-essential Amino Acid Solution (Sigma). C6/36 cells were grown at 28°C in a 5% CO2 incubator ...
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bioRxiv - Genetics 2020Quote: Serial ten-fold dilutions of logarithmic yeast cells were spotted on fresh Synthetic Dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of Methyl methane sulfonate (MMS)(Sigma) and incubated at 30°C for three days ...
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bioRxiv - Cell Biology 2022Quote: ... 1% penicillin/streptomycin and 1% MEM non-essential amino acid solution (all three from Sigma-Aldrich, St. Louis, MO, USA) in 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: (1) rabbit anti-gamma amino butyric acid (GABA; Sigma-Aldrich #A2052, 1:1000 dilution) (2 ...
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bioRxiv - Molecular Biology 2022Quote: ... yejASm lacking the fragment encoding the first 30 amino acids (signal peptide) was PCR-amplified from Sm1021 genomic DNA and cloned into a pET29b(+) vector (Novagen) generating a C-terminal 6His-tag ...
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bioRxiv - Microbiology 2022Quote: ... cells were grown in M9-glucose minimal medium (0.4% D-glucose, 1× MEM amino acids and 1× MEM vitamin) prepared with ingredients from Sigma (57). Antibiotics were used at the following concentrations ...
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bioRxiv - Molecular Biology 2022Quote: ... where individual amino acids concentrations are as defined in Yeast Synthetic Drop-out Media Supplements (Sigma-Aldrich, Saint Louis, Missouri), and 2% Glucose ...
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bioRxiv - Plant Biology 2022Quote: ... Dried samples were then resuspended in 150 µL methanol containing internal standards (15 µM U-13C, 15N amino acid mix, 5 µg/mL 13C2, 15N3 cytosine (Sigma), 10 µg/mL U-13C mannitol and trehalose (Omicron) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yeast strains were grown in synthetic dropout (SD) media containing Yeast Nitrogen Base Without Amino Acids mix (Sigma-Aldrich Y0626) supplemented with 10 g/L glucose ...
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bioRxiv - Microbiology 2022Quote: ... Polar metabolites were resuspended in 170 µL of 100% methanol containing 13C-15N labeled amino acids (30 µM, 767964, Sigma). UHPLC normal phase chromatography was performed using an Agilent 1290 LC stack ...
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bioRxiv - Biochemistry 2023Quote: ... DNA encoding the amino acids 264-1309 of dmIR was subcloned into the vector pBAC™4x-1 DNA (Novagen) downstream of the p10 promoter with the addition of a starting Methionine and C-terminal StrepII-tag ...
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bioRxiv - Microbiology 2024Quote: ... to introduce a KpnI site without changing the amino acid sequence by PCR from pTagRFP-N1 65 and cloning it into pFlag-CMV2 (Sigma). pFlag-TagRFP-KanS ...
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bioRxiv - Genetics 2024Quote: Serial 10-fold dilutions of logarithmic yeast cells were spotted on fresh synthetic dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of methyl methanesulfonate (MMS) (Sigma) and incubated at 30°C for 3 days ...
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bioRxiv - Plant Biology 2023Quote: ... then resuspended in 100% MeOH containing an internal standard mix of isotopically labeled compounds (∼15 µM average of 5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 10 µg/mL 13C-trehalose ...
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bioRxiv - Neuroscience 2023Quote: ... gut samples were treated with D-amino acid oxidase from porcine kidney (PkDAAO) (Catalog# A5222, Sigma-Aldrich, St. louis, MO). The sample was mixed with PkDAAO (15 U/mL) ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich), 1 g/L monosodium glutamate (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Viral inoculum was replaced with 0.22 μm filter-sterilized pH 6.8 growth medium (1X non-essential amino acids [Gibco, MA]/20 mM HEPES, pH 6.8 [Gibco, MA]/5 mM sodium bicarbonate [Sigma-Aldrich, MA]/1X penicillin-streptomycin [Gibco ...
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bioRxiv - Microbiology 2023Quote: The cDNA for AFF4 (also known as MCEF) amino acids 1-715 was cloned into pet-6HIS- 11d plasmid (Novagen) and expressed in E ...
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bioRxiv - Bioengineering 2023Quote: ... Precusor nCys- mEpoR peptide was labeled at its free amino N-terminus as a protected peptide on resin with NHS-Biotin (Sigma) by swelling the resin with DMF having 10 equivalents N,N- Diisopropylethylamine ...
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bioRxiv - Cancer Biology 2023Quote: ... Where indicated an RGD peptide (sequence - Acetyl-Cys-Doa-Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2; Doa = 8-amino-3,6-dioxaoctanoic acid, Millipore Sigma) was added to the cell suspension at a concentration of 0.5 mM ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of deproteinized sample were mixed with a freshly made 20 mg/ml solution of p-dimethyl-amino-benzaldehyde (Ehrlich’s reagent; Sigma # 109762) in glacial acetic acid at room temperature to allow for the formation of a yellow colored product ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: The auto activity of yeast baits on Sc-His due to minimal HIS3 expression was determined by spotting them on Sc-His media containing 0-30 mM 3-Amino-1,2,4-triazole (3-AT) (A8056, Sigma-Aldrich, USA). The minimum concentration of 3-AT that completely inhibits the growth of the bait was considered for the Y1H assay (20mM for pdistal ...
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were grown at 30 °C in minimal synthetic media or agar plates containing 6.8 g/L yeast nitrogen base without amino acids (Sigma-Aldrich), 2 g/L dextrose (Formedium) ...
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bioRxiv - Bioengineering 2023Quote: ... yeast cultures were grown in synthetic complete media containing 0.67% (w/v) Yeast Nitrogen Base Without Amino Acids (Sigma-Aldrich), 1.92 g/L Yeast Synthetic Media Dropout Mix (uracil ...
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bioRxiv - Neuroscience 2024Quote: ... 1x minimum essential media-nonessential amino acids (MEM-NEAA) and 1:1000 1 mg/ml heparin from porcine intestinal mucosa (both Sigma). At day 12 ...
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bioRxiv - Cell Biology 2024Quote: ... 2022) and cells were plated on SD + complete amino acid agar plates with or without 1μg/ml AmphotericinB (Sigma, #A2932). Cells were incubated for two days at 37°C and then imaged.
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid ...