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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... TEER values (Ω * cm2) were calculated as net resistance * membrane area (0.33cm2 for 24-well inserts, Millipore). Only samples with TEER > 200 Ω*cm2 on day 10 were used for stimulation experiments.
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bioRxiv - Neuroscience 2024Quote: ... blocked for 24 hours at 4°C in PBT + 10% normal donkey serum (D9663-10ML, Sigma-Aldrich), washed once with PBT ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated at 28.5 °C and treated at 24 hpf with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to inhibit pigmentation ...
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bioRxiv - Biophysics 2023Quote: ... Organoid-generated forces are relaxed after ∼ 24 hours using 10 µM cytochalsin D (Sigma-Aldrich, St. Louis) and 0.1% triton x-100 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-OHT treated embryos at 24 hpi were anaesthetised by treating with 55 mg/L eugenol (Sigma), screened ...
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bioRxiv - Microbiology 2023Quote: ... After 24 hs cells were stimulated with the indicated concentrations of IFNα A/D (Millipore Sigma, #I4401) or IFNβ (Millipore Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were collected after 24 hours post-infection using RIPA lysis buffer (Sigma, Cat#20-188) supplemented with protease inhibitor (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... gondii strains for 24 h then washed with PBS and fixed with 4% w/v formaldehyde (Sigma) for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was treated with an equal volume of phenol: chloroform: isoamyl alcohol (25:24:1, Sigma) solution to extract proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were separated and placed on Millicell inserts in individual wells of a 24-well plate (Millipore) with 250 µl brain slice medium containing 46.6% minimum essential medium (ThermoFisher Scientific) ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and plated in 24-well plates on coverslips (Laboratory Glassware Marienfeld) coated with poly-L-lysine (Sigma) and laminin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... dissociated neurons were plated into 24-well plates coated with 100μg/ml poly-D-lysine (Sigma-Aldrich) and 10μg/ml laminin (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Tm/+ organoids were cultured for 24 hours in Complete medium supplemented with 1 μg/ml doxycycline (Sigma) before being harvested for RNA extraction.
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bioRxiv - Cell Biology 2024Quote: ... The upper aqueous phase was further purified by phenol/chloroform/isoamyl alcohol (25:24:1) extractions (Sigma) prior to isopropanol precipitation ...
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bioRxiv - Microbiology 2024Quote: ... The total RNA was isolated from 24 h post infected cells using TRI Reagent® (Sigma- Aldrich) according to the protocol described by the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... the TF ORF-carrying mESCs were pretreated for 24 hours with 2 µg/ml doxycycline (#D9891, Sigma) and then lipofected as mentioned in the “Reporter library transfection and pathway perturbations” section.
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bioRxiv - Cancer Biology 2024Quote: HEK293T cells were seeded in 24-wells onto glass coverslips that were coated with laminin (Sigma-Aldrich) (2,5 μg/mL in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were cultured in 24-well plates and incubated with a thiazolyl blue tetrazolium bromide solution (Sigma). The resulting formazan product was measured at 570 nm using a plate reader (Tecan) ...
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bioRxiv - Cell Biology 2024Quote: ... 1×104 cells were seeded in 24-well plates coated with 1:100 laminin (#L2020, Sigma-Aldrich) and the culture media were changed for 21-60 days to neural differentiation media composed by human Neurocult basal media supplemented with human Neurocult differentiation supplement (#05752 ...
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bioRxiv - Developmental Biology 2024Quote: ... After 24 hours (E3.5) the zona pellucidae were removed by brief incubation in acid Tyrode’s solution (Sigma), washed three times in their respective culture medium ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were cultured at 4% haematocrit (using human O+ RBCs) and synchronised with 5% sorbitol (Sigma). For transfection ...
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bioRxiv - Neuroscience 2021Quote: ... P3 and P6 pups were injected intraperitoneally with 50 mg/kg of EdU (5-ethyl-2-deoxyuridine; Sigma). Brains were harvested 2h after injection and processed for frozen tissue sections ...
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bioRxiv - Microbiology 2023Quote: ... VirB or its mutant variants were incubated with 5 mM Tris(2-carboxy-ethyl) phosphine (TCEP; Sigma, USA) for 1 h at room temperature to fully reduce all cysteine residues ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: The 5-HT2A antagonist (R)-(+)-α-(2,3-Dimethoxyphenyl)-1-[2-(4-fluorophenyl)ethyl]-4-piperinemethanol (M100907; Sigma Aldrich) at a dose of 0.5 mg/kg.
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bioRxiv - Evolutionary Biology 2021Quote: ... 2-Methoxy-3-methyl-1,4-BQ was synthesized as follows: 2 mmol 2-Methoxy-3-methyl-1,4-hydroquinone (Sigma Aldrich) and 2 mmol NaIO4 were mixed in a 50 ml round-bottom flask and 10 ml CH2Cl2 and 5 ml of water were then added ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were further washed with TBS and treated with DAB solution for 20 minutes (0.025% 3, 3’-diaminobenzidine tetrahydrochloride; Sigma + 2.5% Nickel Sulphate Hexahydrate ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were resolved on the Discovery HS F5-3 column (2.1 mmI.D. x 150 mmL, 3 μm particle, Sigma-Aldrich), using a step gradient with mobile phase A (0.1% formate ...
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bioRxiv - Biophysics 2022Quote: 1,2-dioleoyl-sn-glycero-3-phosphocoline (DOPC) (TebuBio) and 1,2-dioleoyl-sn-glycero-3-phospho(1’-rac-glycerol) (sodium salt) (DOPG) (Sigma) were dissolved in chloroform ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 3×106 co-transformants was initially screened for growth on synthetic defined media (SD)-Leu-/ Trp-/ Ura-/ His- media containing 20mM of 3-amino-1,2,4-triazole (3-AT, Sigma-Aldrich). Plasmids were isolated from the potential positive colonies ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were immersed in a solution of 0.05% 3-3’diaminobenzidine-4HCl (DAB, Sigma-Aldrich, St Louis, MO) and 0.05% hydrogen peroxide ...
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bioRxiv - Immunology 2020Quote: The following reagents were used in this study: 3-methyladenine (3-MA; M9281; Sigma-Aldrich, St. Louis, MO, USA), dimethylsulfoxide (D2650 ...
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bioRxiv - Molecular Biology 2022Quote: ... fungal hyphae grown (for 2-3 weeks) in liquid M-C medium without Fe were exposed 3 days to 0.5 mM ferrozine (Sigma). This was done by replacing the liquid medium by 20 ml of a freshly prepared liquid M-C medium without Fe and supplemented with 0.5 mM ferrozine ...
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bioRxiv - Developmental Biology 2020Quote: ... paws were incubated twice for 5min with methanol 50% BABB (1/3 benzylalcohol and 2/3 benzylbenzoate, from Sigma), and then three times in pure BABB for 5min each (or until the sample is cleared) ...
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bioRxiv - Microbiology 2021Quote: ... harvested at day 3 and clarified by centrifugation at 350 x g for 15 minutes (Sigma 3-16K centrifuge). Viral stocks were concentrated by centrifugation at 11 ...
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bioRxiv - Neuroscience 2020Quote: ... Sigma)10,42 and the metabotropic glutamate receptor antagonist DL-2-Amino-3-phosphonopropionic acid (AP-3) ([1mg/kg] in PBS, stock made in 1M NaOH diluted, pH 7.4; Sigma)10,43 ...
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bioRxiv - Biophysics 2022Quote: Peak fractions of the PEAK3/14-3-3 complex were pooled and concentrated using an Amicon Ultra-0.5 30k MWCO centrifugal filter (Millipore). Immediately before preparing cryo-EM grids ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were separated on a Discovery HS F5-3 column (2.1 mm i.d. × 150 mm long, 3 µm particle size, Sigma–Aldrich) at a flow rate of 0.25 ml min−1 with a step gradient of mobile phase A (0.1% formic acid ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were immersed in a solution of 0.05% 3-3’diaminobenzidine-4HCl (DAB, Sigma-Aldrich, St Louis, MO) and 0.05% hydrogen peroxide ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were separated on a Discovery HS F5-3 column (2.1 mm i.d. × 150 mm, particle size 3 μm, Sigma-Aldrich) using mobile phase A (0.1% formic acid/water ...
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bioRxiv - Bioengineering 2023Quote: ... coverslips were thiol-silanised by submerging for 3 hrs in a 10% solution of (3-Mercaptopropyl)trimethoxysilane (Sigma-Aldrich) in toluene before tempering by incubating at 100°C for 1 hr.
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bioRxiv - Neuroscience 2022Quote: ... 1 x 90 min) in 3% PBT (3% Triton-X-100 [CAS: 9002-93-1, Sigma Aldrich] in PBS) on ice ...
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bioRxiv - Microbiology 2023Quote: ... was subjected to centrifugation through a 3 kDa cut-off membrane filter (Amicon Ultra-0.5 3-kDa Ultracell, Millipore) at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in 3 % BSA/PBS and incubated with anti-ZEB1 antibodies (Sigma, HPA027524, 1:200 in 3 % BSA/PBS). After washing and incubation with Alexa594-conjugated secondary antibodies (Life Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves with expressing effectors were immersed in 1 mg/ml DAB (3, 3′-diaminobenzidine) solution (Sigma-Aldrich, USA) at 25°C for 8 h ...
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bioRxiv - Immunology 2023Quote: ... 2 and 3 were detected with another multiplex assay (MILLIPLEX MAP TGFß Magnetic Bead 3 Plex Kit; Merck Millipore).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sections were then rinsed with PBS 3 × 10 minutes and blocked with 3% BSA (Bovogen Biologicals, BSAS 1.0) + 0.25% Triton (Sigma Aldrich) in 1x PBS (pH 7.2 ...
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bioRxiv - Genetics 2024Quote: ... Positive interactions were scored by the appearance of white-coloured colonies on a synthetic defined medium containing 6 µg mL-1 adenine and/ or by the growth in the presence of 2 mM 3-Amino-1,2,4-triazole (3-AT) (Cat. No. A8056, Sigma-Aldrich).
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bioRxiv - Genetics 2024Quote: ... cells were transferred into SFEM II supplemented with 3 U/mL hEPO (SCT) and 3% AB human serum (Sigma) for an additional 5 days of culture ...
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bioRxiv - Molecular Biology 2024Quote: 1,2-dioleoyl-sn-glycero-3-phosphocoline (DOPC) (TebuBio) and 1,2-dioleoyl-sn-glycero-3-phospho(1’-rac-glycerol) (sodium salt) (DOPG) (Sigma) were dissolved in chloroform ...
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bioRxiv - Cancer Biology 2024Quote: The caspase 3/7 activity assay was carried out using the caspase 3/7 activity assay kit from Sigma, following the manufacturer’s protocol ...