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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Mouse IgG2a Isotype Control Antibody Biotin 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... or vehicle DMSO as negative control (Sigma-Aldrich). PGPC17 and variant-corrected CMs were included as controls ...
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bioRxiv - Cell Biology 2023Quote: ... GAPDH was used as internal control (Millipore: #MAB374).
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bioRxiv - Cancer Biology 2023Quote: ... and siRNA Universal Negative Control #1 (Sigma # SIC001).
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bioRxiv - Molecular Biology 2023Quote: ... SHC002 Control (all from Sigma Aldrich, Burlington, MA). Western blot antibodies included ...
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bioRxiv - Cancer Biology 2023Quote: ... or vehicle control (15% Kolliphor HS15 (Sigma 42966), 60% Phosal propylene glycol (MP ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with DMSO (vehicle control, Sigma), 5 μM Nutlin-3a (Selleck Chemicals) ...
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bioRxiv - Immunology 2021Quote: ... Serum IgG subclasses were tested with isotype-specific murine anti-human IgG subclasses (Sigma-Aldrich, S:t Louis, MA).
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bioRxiv - Developmental Biology 2021Quote: ... The abundance of H3K9ac and H3K9bhb was calculated in relation to the loading control histone H3 antibody (Sigma, cat. # H0164; diluted 1:10,000) ran in parallel.
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bioRxiv - Plant Biology 2019Quote: Four grams of frozen shoot tissue from 24-hour Pi deficiency or control treatment was used to perform chromatin immunoprecipitation (ChIP) as previously described (Zahraeifard et al., 2018) using the antibody (Millipore; lot number 2648189) against H3K4me3 and input genomic DNA as a control ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Genetics 2019Quote: ... paraffin-embedded and stained using anti-RAS (G12S) mouse monoclonal antibody (NewEast, 26186) followed by incubation with HRP-conjugated corresponding secondary antibody (Sigma-Aldrich). The expression levels were evaluated by H-score method ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated with secondary anti-Rabbit IgG-Gold coupled antibody (1/10 in BHN1) (Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat (Sigma, G7652), washed twice in BHN2 and HN (50 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit antibody against Parvalbumin (1:500, PA1-933, Thermo Fischer Scientific) and Mouse antibody against GAD67 (1:500, MAB5406, Sigma-Aldrich) in staining buffer (0.2% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were incubated overnight at 4 °C with primary antibodies: mouse monoclonal antibody against α-actinin (1:1,000; Millipore, MAB1682), mouse monoclonal antibody against G6PD (1:250 ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with blocking solution (1% bovine serum albumin and 0.2% Triton-X in 0.01 m PBS) for 1 hour at room temperature before primary antibodies were applied in blocking solution [mouse anti-parvalbumin antibody (Millipore, MAB1572) at 1:2000 overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were used at the following dilutions: horseradish peroxidase coupled secondary antibodies (α-mouse and α-rabbit, Sigma, 1:20,000), anti-mouse Oregon Green (Molecular Probes ...
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bioRxiv - Biochemistry 2022Quote: ... Then cells were stained with anti-mouse IgG Taxes red conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACS Aria II (BD ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were immunoblotted with HRP-conjugated Flag antibodies (Monoclonal ANTI-FLAG® M2-Peroxidase HRP antibody produced in mouse, A5892; Merck-Millipore), HRP-conjugated HA antibodies (12013819001 ...
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bioRxiv - Microbiology 2019Quote: ... The TopA protein was subsequently detected using primary rabbit polyclonal anti-TopA antibodies and mouse secondary anti-rabbit IgG antibodies conjugated with alkaline phosphatase (Sigma Aldrich), according to the procedure described previously (Szafran et al. ...
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bioRxiv - Microbiology 2020Quote: ... or anti-gL (rabbit polyclonal) antibodies and detected using anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase (Sigma-Aldrich) and Pierce ECL-Western blotting substrate (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663 ...
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bioRxiv - Immunology 2021Quote: ... Then cells were stained with anti-mouse IgG Taxes red-conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACSAria II (BD ...
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bioRxiv - Neuroscience 2020Quote: ... the nitrocellulose membranes underwent a 1h blocking step in Rockland buffer before 6h incubation with primary antibodies (for all antibody RRIDS, see Key Resources Table): mouse α-KCC2 (1:1000, Millipore), rabbit α-phospho-KCC2-S940 (1:1000 Aviva Systems Biology) ...
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bioRxiv - Neuroscience 2023Quote: ... The following commercial primary antibodies were used: rabbit anti-Olig2 antibody and mouse anti-panNav mAb (clone K58/35) purchased from Sigma-Aldrich Chimie ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at room temperature with a combination of 2 different primary antibodies: a mouse monoclonal anti-PCNA antibody (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunostained with rabbit anti-SON antibody (1:200 dilution, Novus) or mouse anti-SRRM2 antibody (1:200 dilution, Sigma Aldrich) for 1h at room temperature followed by 3 times wash with DPBS ...
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bioRxiv - Microbiology 2024Quote: ... HRP-conjugated anti-mouse secondary antibody (57404) was purchased from SRL and anti-rabbit secondary antibodies were purchased from Sigma (A8275). Alexa Fluor 488 goat anti-mouse (A11001) ...
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bioRxiv - Microbiology 2024Quote: ... with the same combination of primary and secondary antibodies: mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and rabbit anti-HA tag antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Genetics 2024Quote: The primary antibodies used in this paper were mouse anti-tubulin monoclonal antibody DM1A at 1:50 conjugated to FITC (Sigma-Aldrich), mouse anti-tubulin monoclonal antibody E7 at 1:200 (Developmental Studies Hybridoma Bank) ...
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bioRxiv - Microbiology 2023Quote: ... Goat anti-IgG rabbit alkaline phosphatase conjugated secondary antibody (A9919) and goat anti-IgG mouse alkaline phosphatase conjugated secondary antibody (A2429) were purchased from Sigma Aldrich.
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-phosphoserine or anti-threonine antibody followed by 1:20000 anti-mouse antibody and detected using Luminata Forte Western reagent (Millipore, USA). Same protocol was followed for in vitro kinase assay using the mutated osbzip1.1 protein ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2, 1:100, Millipore; mouse anti-GRIN2BB ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... anti-p24 mouse monoclonal antibody (1: 500; MAB7360, R&D, Minneapolis, MN, USA) and anti-ZCCHC3 mouse monoclonal antibody (1: 500; SAB1408147, Sigma-Aldrich). The following secondary antibodies were used ...
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bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
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bioRxiv - Genetics 2023Quote: ... then incubated in the primary antibody cocktail for 48-72 hours (1:1000 dilution of tyrosine hydroxylase mouse monoclonal antibody, MAB318, Millipore Sigma). The secondary antibody was biotinylated horse anti-mouse ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... MH27 mouse monoclonal antibody (from supernatent of cells provided by R. Waterston) was used with rhodamine-conjugated goat anti-mouse secondary antibody (Sigma-Aldrich) to stain fixed animals of different stages.
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bioRxiv - Developmental Biology 2023Quote: ... The cells were incubated with primary antibody solution 1:8000 dilution of acetylated α tubulin mouse monoclonal antibody (Sigma Aldrich T7451) and a 1:125 dilution of ift88 rabbit monoclonal antibody (Abcam ab184566 ...
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bioRxiv - Cell Biology 2023Quote: ... and of PTMs (tyrosinated tubulin: YL ½ antibody, ab6160; rat monoclonal, Abcam; acetylated tubulin: T6793 antibody, clone 6-11B-1, mouse, Sigma-Aldrich; polyglutamylated tubulin ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with secondary antibody in PBS (1:250) anti-Mouse IgG (whole molecule) –fluorescein isothiocyanate (FITC) antibody produced in goat (Sigma, F9006) in dark for 1 h ...