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Citations for Millipore Sigma :
2151 - 2200 of 2850 citations for GDNF Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Human adenocarcinoma alveolar basal epithelial cells (A549, ATCC) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: Human colorectal adenocarcinoma cell lines HT29 and HCT116 (both European Collection of Cell Cultures through Sigma-Aldrich, Dorset, UK) were grown in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biochemistry 2021Quote: The hnRNP L_RRM2 fragment (residues189-286) was amplified from the human brain cDNA library and cloned into a modified pET28a (Novagen) vector without a thrombin protease cleavage site (termed p28a) ...
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed with PBST and incubated with a goat anti-human Fc−peroxidase antibody diluted 1:5,000 (Sigma) in blocking buffer for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were then washed and incubated with a 1:5,000 dilution of peroxidase-conjugated anti-human Fc antibody (Sigma) for 1 hr ...
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bioRxiv - Cancer Biology 2021Quote: ... digested splenic cells were then cultured in MEM-α with 5% human platelet lysates (PLTMax; Cat# SCM142, Millipore Sigma) at a density of 2-5 × 104 cells/mL in culture flasks for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 50% Neurobasal Medium, 0.5% N2 supplement, 1% B27 without vitamin A [Life Technologies, 12587-010], 0.025% [v/v] human insulin [Sigma, I9278] ...
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bioRxiv - Cancer Biology 2022Quote: ... human tumor slides were stained by immunohistochemistry with an antibody against Tenascin-C (1:300, EMD Millipore, Cat. ab19011), a strain-sensitive ECM protein known to be present in LUAD [9 ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 (human embryonic kidney, ATCC) and U2OS (osteosarcoma, ATCC) cells were grown in DMEM (Dulbecco’s modified Eagle’s medium, Sigma-Aldrich) supplemented with 10% FBS and 2 mM L-glutamine.
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bioRxiv - Bioengineering 2019Quote: ... The antibodies used were mouse anti-human HSA (1:500; clone HSA-11, A6684; Sigma Aldrich; St. Louis, MO) and 10 nm colloidal gold-conjugated goat anti-mouse IgG (1:40 ...
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bioRxiv - Pathology 2020Quote: ... the suspension adjusted to 1 × 106 parasites/mL in PBS and was incubated with 20% human complement (Sigma Aldrich). After 30 min ...
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bioRxiv - Systems Biology 2020Quote: ... Metabolic hormones were measured using MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay (HMHEMAG-34K, Millipore). Cardiovascular risk markers in plasma were measured using MILLIPLEX MAP Human Cardiovascular Disease (CVD ...
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bioRxiv - Developmental Biology 2020Quote: Cells were seeded onto glass coverslips coated with 5 μg/mL human plasma fibronectin purified protein (Millipore Sigma #FC010). At ~90–100% confluency ...
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bioRxiv - Biochemistry 2019Quote: ... ti-sodium citrate and sodium hydroxide were purchased from Merck Company and Human serum albumin (96%) and ANS (1-anilino-8-naphthalene sulfonic acid) were purchased from Sigma-Aldrich.
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bioRxiv - Cell Biology 2020Quote: Human 293T and mouse P19 cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... The cGAS knockdown for RNA interference was achieved using Mission shRNA-encoding lentivirus directed to human cGAS mRNA (Sigma; GenBank/EMBL/DDBJ accession no ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
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bioRxiv - Molecular Biology 2020Quote: Human FOP fibroblasts (Coriell Institute, NJ) were grown to confluence in DMEM supplemented with 10% FBS (Sigma-Aldrich, MO) and then seeded into 96-well plates (Costar® 3610 ...
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bioRxiv - Molecular Biology 2021Quote: Human genomic DNA was isolated from HeLa cells using a GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich). Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... at 14:30 and 48 hours later with 5 IU of human chorionic gonadotropin (5 IU, hCG; EMD Millipore). At the time of hCG injection ...
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bioRxiv - Genetics 2020Quote: ... The cauda epididymis was nicked three times to allow sperm to swim out and placed in a 2ml round bottom Eppendorf tube filled with 1.1ml of 37°C Human Tubal Fluid media (Millipore). Sperm were placed in a 37°C incubation chamber and allowed to swim out for 10 minutes before the cauda epididymis was removed ...
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bioRxiv - Immunology 2019Quote: ... Cells were blocked overnight at 4°C with 150µl blocking solution per well (5% (v/v) human serum (Sigma), 5% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... pH = 7.6) containing 0.01% Triton X-100 (TBS-T) and then blocked with 2% human serum albumin (HSA; Sigma-Aldrich) and 10% NGS in TBS-T for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... or 48-well plates (with or without 7×7mm glass coverslips) pretreated with 50 µg/mL human fibronectin (Millipore).
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bioRxiv - Neuroscience 2019Quote: After extensive washes in PB and 0.05 M Tris-buffered saline (pH 7.4; TBS) sections were blocked in 1 % human serum albumin (HSA; Sigma-Aldrich) in TBS ...
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bioRxiv - Immunology 2020Quote: Human monocyte-derived macrophages (hMDMs) were differentiated from buffy cones from healthy donors using gradient separation (Histopaque 1077, Sigma). Following Histopaque separation ...
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were removed and the remaining monocytes were maintained in DMEM with 5% human serum (HS; Millipore) and penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: ... or a non-targeting control shRNA (TRCN0000231489) with no targets in the human genome were obtained from Sigma-Aldrich. The SLFN11 expression vector was obtained from VectorBuilder along with an empty vector control with the same lentiviral backbone ...
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bioRxiv - Biochemistry 2021Quote: ... Primary rabbit polyclonal anti-(human Nav1.5), (residues: 1978-2016) (#ASC-013, Alomone Labs) and rabbit polyclonal anti-(actin) (A2066, Sigma), both used at a concentration of 1:200 were followed by a one-hour incubation with secondary Goat anti-(Rabbit IgG-HRP conjugate ...
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bioRxiv - Immunology 2021Quote: ... The albumin analyte was selected and measured using MILLIPLEX MAP Human Kidney Injury Magnetic Bead Panel 2 (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... plates were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Sigma-Aldrich, 5µg/ml in PBS, 1h at RT).
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bioRxiv - Developmental Biology 2020Quote: ... Female frogs (4-15 years old) were injected subcutaneously with 300-700 units of human chorionic gonadotropin (hCG; Sigma), depending on weight and age ...
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bioRxiv - Developmental Biology 2020Quote: ... sperm were collected from the vasa deferentia and then capacitated for 1.5 hours in human tubal fluid (HTF) medium (Millipore). Sperm cells were then incubated with intact cumulus-oocyte complexes or zona-free oocytes for 5 hours ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells (a human embryonic kidney cell line; ATCC CRL-1573) were maintained in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) containing fetal calf serum and antibiotics.
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were removed and the remaining monocytes were maintained in DMEM with 5% human serum (HS; Millipore) and penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... The bound antibodies were revealed using a HRP-conjugated goat antibody to mouse Ig absorbed with human Ig (Sigma) (1:4,000 dilution) ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Immunology 2021Quote: ... 0.3 x 106 primary human keratinocytes were seeded directly after transfection in 500 µl keratinocyte medium supplemented with 1.5 mM CaCl2 (SIGMA) into the insert ...
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bioRxiv - Immunology 2020Quote: ... were incubated overnight at 4°C with 50 μL of a solution of in 75 mM sodium carbonate (pH 9.6) containing either: 2.5 μg/ml aggregated human IgG (Sigma-Aldrich) for CP ...
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bioRxiv - Biophysics 2021Quote: Human serum albumin (fraction V) 98% and 5,5’-dithiobis-2-nitrobenzoic acid (DTNB) were purchased from Sigma Aldrich (USA) and used without any prior purification ...
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bioRxiv - Genomics 2020Quote: ... Cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% AB human serum (Sigma Aldrich), 100 U/mL recombinant human IL-2 (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... were co-expressed with the C-terminal domain of human DNMT3L (residues 178–386 of NCBI accession NM_175867) on a modified pRSFDuet-1 vector (Novagen), in which the DNMT3B or DNMT3A sequence was preceded by a hexahistidine (His6 ...
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bioRxiv - Neuroscience 2021Quote: Dissociated human iPSC-derived motor neurons were re-plated on poly-D-lysine (PDL; 100 µg/ml; Sigma-Aldrich) and CSPG-(0.2 ng/ml ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Binding of cGAS and dsDNA in human and mouse brain sections was determined by PLA kit (DUO92101, Sigma-Aldrich) accordingly to the manufacture’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... NHP and human hepatocytes from xenograft FRG mice and NHP tissues was extracted using the TriReagent (Sigma, Cat# T9424)-chloroform protocol previously published.35,36
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bioRxiv - Cell Biology 2022Quote: ... The same ex vivo culture system was used to assess human lung cytokine responses to lipopolysaccharide (LPS, L5668, Sigma) stimulation by ELISA ...
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bioRxiv - Cell Biology 2022Quote: ... The following day the cells were washed with PBS and media changed to human osteoblast differentiation media (Sigma-Aldrich). Osteoblast differentiation media was changed every 3 days for a period of 14 days as per the manufacturer’s guidelines ...