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Citations for Millipore Sigma :
2151 - 2200 of 9976 citations for Cow Serine protease HTRA2 mitochondrial HTRA2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 0.25X protease inhibitor cocktail EDTA free (Roche, Sigma), and 1X phosphatase inhibitor cocktail (Santa Cruz Biotechnology) ...
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Deletion of adipocyte Sine Oculis Homeobox Homolog 1 prevents lipolysis and attenuates skin fibrosisbioRxiv - Molecular Biology 2024Quote: ... containing Halt Protease and Phosphatase inhibitor(Sigma Aldrich). Samples were loaded in reducing Sodium dodecyl-sulfate (SDS ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with a protease inhibitor cocktail (Sigma-Aldrich). Cell lysates were incubated on ice for 30 minutes ...
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bioRxiv - Biophysics 2024Quote: ... and EDTA-free protease inhibitor (SIGMAFASTTM, Sigma-Aldrich). Cells were lysed and the lysate was centrifuged at 12,000 g for 10 minutes at 4°C to remove unbroken cells and the supernatant then ultracentrifuged for 2 h at 200,000 g ...
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bioRxiv - Plant Biology 2024Quote: ... 1x cOmplete EDTA-free protease inhibitor tablets (Sigma) and 0.2% v/v IGEPAL) ...
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bioRxiv - Cell Biology 2024Quote: ... and supplemented with a protease inhibitor cocktail (Sigma), 5 mM NaF ...
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bioRxiv - Immunology 2024Quote: ... 2X Roche Complete Protease Inhibitor (Millipore Sigma# 11873580001). 100X of KDAC inhibitor cocktail (100 µM TSA ...
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bioRxiv - Cell Biology 2024Quote: ... and a protease inhibitor cocktail (PIC0002, Sigma-Aldrich) was added to prevent protein degradation ...
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bioRxiv - Genetics 2024Quote: ... 5 mM DTT and protease inhibitor cocktail (Sigma). Following incubation on ice for 30 mins ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.4 and supplemented with protease inhibitors (Sigma, Complete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2024Quote: ... with a protease inhibitor cocktail (Sigma-Aldrich, # 04693159001). Verification of CRISPR knockouts was confirmed using western blot ITGB6 antibodies for human (R&D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... 1x protease inhibitor cocktail (Millipore Sigma Cat# 11873580001). Homogenized samples were incubated on ice for 30 min and centrifuged at 12000 rpm for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 EDTA-free protease inhibitor tablet (Sigma-Aldrich), pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... and protease inhibitor cocktail (D2714, Sigma-Aldrich, Belgium).
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bioRxiv - Physiology 2024Quote: ... and one cOmplete protease inhibitor tablet (Sigma 04693159001)) ...
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bioRxiv - Immunology 2024Quote: ... containing a protease inhibitor cocktail (P8340, Sigma-Aldrich). Protein concentrations were determined using a BCA Protein Assay Kit (23227 ...
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bioRxiv - Cell Biology 2020Quote: ... were used to produce chromatin lysates and the following protease inhibitors were added to each: 1x protease inhibitor cocktail (Sigma, P8340) and 50μg/ml PMSF (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.5% deoxycholic acid) containing a protease and phosphatase inhibitor cocktail (cOmplete™ Protease Inhibitor Cocktail and PhosSTOP™ from Sigma). Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to an Immobilon polyvinylidene fluoride membrane (EMD Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... The slurry was then equilibrated at 37°C and 200 ug of DNase and protease-free RNase A and 40 µl of protease inhibitor cocktail (Sigma Aldrich) was added and the column was re-sealed and incubated with gentle rocking at 37°C for one hour ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Cells were lysed by six rounds of liquid nitrogen freeze/thaw lysis in in low salt TBS (50 mM Tris, 50 mM NaCl, pH 7.5) and protease/phosphatase inhibitors (Plant Protease Inhibitor Cocktail (Sigma, Burlington, Massachusetts), 1 mM Benzamidine ...
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bioRxiv - Developmental Biology 2020Quote: ... Dissected matured primary follicles were also soaked in vitro with protease inhibitors (PI, 1X concentration in water; cOmplete™ Mini Protease Inhibitor Cocktail, Sigma). Ovaries from female mosquitoes at 96 hours PBM were dissected and placed in water containing PI at different time points after ovary dissection ...
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bioRxiv - Microbiology 2020Quote: Cell lysates were prepared using Radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with protease inhibitors (cOmplete Protease inhibitor cocktail, Sigma-Aldrich, 11697498001) and 50mM 1,4-Dithiothreitol (DTT) ...
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bioRxiv - Neuroscience 2022Quote: ... brains homogenized in 1xPBS containing 1% SDS and protease inhibitors (Complete EDTA-free Protease Inhibitor cocktail, Roche, Sigma Aldrich, Cat#11873580001) and boiled for five min ...
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bioRxiv - Biochemistry 2022Quote: ... The cell pellets were resuspended in Permeabilization Buffer supplemented with 2 mM calcium chloride and protease inhibitors (cOmplete Mini EDTA-free Protease Inhibitor Cocktail, Sigma, 11836170001), vortexed briefly ...
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bioRxiv - Microbiology 2021Quote: ... 1% NP-40) in presence of Protease cocktail inhibitor (cOmplete™, EDTA-free Protease Inhibitor Cocktail, Sigma Aldrich, catalogue number 11873580001). Protein lysates were left rotating on-wheel at 4 °C for 15 min and cleared by 15 min of centrifugation at 4°C at 13200 (max speed) ...
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bioRxiv - Molecular Biology 2020Quote: ... containing protease and phosphatase inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail and PhosSTOP from Roche/Sigma, cat. no. 11836170001 and 4906837001), in a pre-chilled 2-mL glass Dounce homogenizer ...
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bioRxiv - Systems Biology 2021Quote: ... 10 mL of lysis buffer (IBA LifeSciences Buffer W supplemented with 0.5% NP-40 substitute and one tablet of protease inhibitor (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Sigma Millipore) was added to the thawed pellet ...
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bioRxiv - Microbiology 2020Quote: ... The virus-containing pellet was solubilized in 1% Triton X-100 in PBS and protease inhibitors (EDTA-free EASYpack Protease Inhibitor Cocktail (Sigma-Aldrich) and finally equilibrated in SDS-PAGE loading buffer for western blot analysis.
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bioRxiv - Genomics 2021Quote: ... minced tissue was transferred to GentleMACS C tubes (Miltenyi, 130-096-334) containing protease solution (10mg/ml Bacillus Licheniformis protease (CAP) (Sigma, P5380) in DPBS supplemented with 5mM CaCl2 and 125U/ml DNaseI (Stemcell ...
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bioRxiv - Genomics 2019Quote: ... a protease solution (5mM CaCl2 [Invitrogen; AM9530G], 10mg/mL B. Licheniformis protease [Sigma; P5380], 125U/mL DNase I [Sigma; D5025], 1xDPBS) was prepared for each kidney.
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bioRxiv - Cell Biology 2024Quote: ... Cells were then scrapped in cold PBS supplemented with protease inhibitors (Protease inhibitor cocktail set III, EDTA-free, Merck/Sigma-Aldrich) supplemented with glycine and isolated by centrifugation ...
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bioRxiv - Pathology 2023Quote: Cells were scrapped and lysed in RIPA buffer (RIPA 2X, Biotech RB4476) supplemented with phosphatase inhibitor (PhosSTOP Easypack, Roche0490687001) and protease inhibitors (protease inhibitor cocktail, Sigma Aldrich). Lysates were centrifuged at 13500g for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... cell pellets were lysed on ice in STET lysis buffer supplemented with protease inhibitors (Protease Inhibitor Cocktail, Sigma Aldrich, Product P8340). The SP3 sample preparation was performed according to a previously published protocol26 using 70% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... and centrifuged at 4°C at 400G for 5 minutes followed by resuspension of the pellet in 500 μl of protease solution (10 mg/ml BI protease (Sigma, P5380), 125 U/ml DNase ...
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0 and 0.15 M NaCl supplemented with 1.0 mM final concentration of phenylmethylsulfonyl fluoride protease inhibitor or a tablet of EDTA-free protease inhibitor cocktail (Sigma-Aldrich, Germany). The cells were lysed with 35 cycles of sonication at 4 °C on ice using 50% amplitude and 25 sec sonication pulse with 1.5 min interval ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested in dCas9 lysis buffer (50 mM NaxPO4 pH 7.0, 300 mM NaCl and protease inhibitors (cOmpleteTM EDTA-free Protease Inhibitors (Sigma-Aldrich #5056489001) plus 0.3 mM PMSF) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% SDS and 0.5% sodium deoxycholate] supplemented with protease inhibitors [1mM PMSF and 1 × mammalian protease inhibitor cocktail (Millipore Sigma, P8340) and phosphatase inhibitors (1mM Na3VO4 and 10mM NaF) ...
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bioRxiv - Developmental Biology 2023Quote: 10-12 somite stage TgBAC(hand2:EGFP)pd24 uninjected and rtf1 morpholino-injected zebrafish embryos58 were nutated in 10 mg/ml protease (Protease from Bacillus licheniformis, Sigma P5380) in DMEM/F12 media for 30 minutes at 4°C and pipetted to dissociate cells ...
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bioRxiv - Cell Biology 2024Quote: ... glycerol and 10% sodium dodecyl sulfate (SDS)) supplemented with a protease and phosphatase inhibitor cocktail (Complete 1x protease inhibitor (Sigma-Aldrich), 1 mM sodium orthovanadate ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mM β-Mercaptoethanol as well as freshly added 0.1 % Triton X-100 and protease inhibitor (cOmplete ™ EDTA-free protease inhibitor cocktail, Sigma-Aldrich) and lysed via French Pressure Cell (Thermo IEC) ...
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bioRxiv - Neuroscience 2024Quote: ... cell pellets were lysed in 2x RIPA buffer containing protease inhibitors (cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail; Millipore Sigma 11836170001) and 1.25 mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: IGF1 levels were measured in serum and SSPC culture supernatant using ELISA (Sigma-Aldrich, RAB-0229) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were captured in ELISA plates pre-coated with 5 µg/mL of Avidin (Sigma) in Na2CO3/NaHCO3 buffer pH 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... CCL2 and CCL5 protein levels were quantified using a sandwich ELISA procedure (Sigma, Cat# RAB0055 & RAB0077) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... as well as BG505 Env-NP expression by ELISA (NP capture: Galanthus nivalis lectin (GNL, Sigma) / NP detection ...
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bioRxiv - Immunology 2020Quote: ... The pro-inflammatory cytokines in the supernatant were analyzed by multiplex ELISA (Millipore, HCYTMAG-60K-PX29) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Plasma cytokines and cytokines secreted in cell culture media were measured using ELISA (Luminex 200; Millipore) by following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Nunc 96-well ELISA plates were coated with 50 µl of 2 µg/ml SA (Sigma) in borate saline buffer (100 mM boric acid ...
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bioRxiv - Neuroscience 2022Quote: ... sandwich ELISA of tau-5 coating antibody (20 µg/ml; Millipore Cat# 577801-100UG RRID: AB_212534) with either biotin-conjugated HT-7 (for human tau ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed in ELISA wash buffer (0.01M PBS with 0.05% Tween 20; Sigma; P7949) for 4x 2 minutes on an orbital shaker ...