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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for 6 Nitro 2 oxo 2H chromene 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma-Aldrich) and phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich). Protein concentration was measured with BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... The coverslips were then immersed in 2% (v/v) (3-Aminopropyl)triethoxysilane (Sigma A3648) prepared in acetone for 2 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Cells were split every 2-3 days with trypsin (Sigma, Saint Louis, Missouri, USA) to maintain a subconfluent monolayer.
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bioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich) and phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich). Protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... concentrated to 2 ml using an Amicon concentrator with a 3 kDa cutoff (Millipore), and applied into a Superdex 75 (16/600 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 2× M63 buffer was made by adding 3 g KH2PO4 (Sigma-Aldrich cat. P5655), 7 g K2HPO4 (Sigma-Aldrich cat ...
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bioRxiv - Cell Biology 2022Quote: ... Triple Dynamin (Dyn1/2/3) knockout was induced by 1 μM 4-hydroxytamoxifen (Sigma) as previously described44 ...
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bioRxiv - Systems Biology 2022Quote: ... and phosphatase inhibitor cocktail 2 and 3 (1:100, P5726 and P0044, Sigma-Aldrich). For immunoprecipitation (IP) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were subcultured every 2-3 days using trypsin (Sigma, Saint Louis, Missouri, USA) to maintain a subconfluent cell layer.
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... or 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (Sigma), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1X protease inhibitor and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich), for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: 3-4 nl of 2 mg per mL Rhodamine B isothiocyanate-Dextran (70kDa, Sigma) dissolved in PBS and 5 mM HEPES were injected into the sinus venosus of 30 hpf embryos ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 3 μM SHP1/2 phosphatase inhibitor (NSC-87877, Sigma-Aldrich) for 20 minutes prior to target particle addition ...
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bioRxiv - Immunology 2020Quote: ... and cyclophosphamide (#C7397, 100 mg kg-1 from day −3 to −2; Sigma-Aldrich) intraperitoneally ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed with 3 × 100 µL of 2% bovine serum albumin (BSA, Sigma) in D-PBS+CM and blocked in that same solution for 2 hours at room temperature (RT) ...
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bioRxiv - Developmental Biology 2022Quote: Myoblasts were transfected with either (final concentrations) 10 µM 2’,3’-cGAMP (Sigma Aldrich), 2 µg/mL poly(I:C ...
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bioRxiv - Microbiology 2022Quote: ... coverslips were incubated in a 2% solution of (3-Aminopropyl)triethoxysilane (APTES, Sigma-Aldrich) in acetone with agitation for two minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma. 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine (CMH ...
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bioRxiv - Neuroscience 2022Quote: ... and 1.0% (v/v) phosphatase inhibitor cocktail #2 and #3 (Sigma-Aldrich P5726, P0044). Tissue homogenates were centrifuged at 14,000×g at 4°C for 20min and supernatants collected and stored at -80°C before initiating the protein assay.
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were transformed into pKM208 carrying Rosseta 2 (Millipore Sigma 71400-3) strain by electroporation ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM Benzamidine) containing phosphatase inhibitor cocktail 2 and 3 (Sigma, P5726 and P0044). Supernatants containing proteins were collected after centrifugation at the highest speed for 15 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with a cocktail of 1:100 phosphatase inhibitors cocktail 2 and 3 (Sigma/Merck ...
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bioRxiv - Microbiology 2023Quote: ... 100 µM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine 5’-triphosphate (BzATP) (#B6396, Sigma-Aldrich), 20 µM MCC950 (#inh-mcc ...
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bioRxiv - Bioengineering 2023Quote: The mice were anaesthetized with isoflurane (induction 5%, maintenance 2−3%; Sigma-Aldrich, USA) and secured in a stereotaxic instrument (RWD ...
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bioRxiv - Cell Biology 2022Quote: MCF10A cells (3×106 cells per condition) were fixed with 2% formaldehyde (Sigma, #252549) in PBS with 10% FBS for 10 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay was used to analyze the cell viability of SH-SY5Y cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and Phosphatase Inhibitor Cocktails 2 and 3 (Sigma- Aldrich cat. P5726 and cat. P0044). Pellets were resuspended in lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: The 3-(4,5-dimethylthiazo1-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Merck Sigma, Burlington, MA) reduction assay was used to quantify cell proliferation ...
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bioRxiv - Biochemistry 2024Quote: ... and 2’,3’-cGAMP (CAS no. 1441190-66-4) were obtained from Sigma-Aldrich and used without further purification.
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bioRxiv - Microbiology 2024Quote: ... MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was obtained from Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μL each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044)) and lysate cleared by centrifugation at 17,000g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Biochemistry 2022Quote: ... they were cultivated 72 hours in the presence of 2 μM DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) (Sigma-Aldrich) to inhibit the synthesis of glucosylceramide-based GSLs 75 and incubated with fluorescent SaroL-1 as previously described ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Mice were subjected to cutaneous immune challenge by applying 20 µl of 3-5% 4- Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in 1:2 acetone:olive oil ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... boric acid and ascorbic acid as well as reagents required for 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) synthesis were obtained from Sigma-Aldrich (Bangalore, India). Acetone was obtained from Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Bioengineering 2024Quote: ... each well was filled with 400 µL of RPMI-B27 supplemented with 10µM Y-27632 and 5 mg/mL 6-aminocaproic acid (Sigma-Aldrich, cat. no. A7824). On day 1 ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... inhibitor MG-132 (474790), 2-deoxy-D-glucose (2-DG, D6134), and palmitic acid (C16:0, P9767) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Briefly ...
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bioRxiv - Immunology 2021Quote: Blood was extracted from hBLT mice at 3 and 5-6 weeks post-infection and lysed with Red Blood Cell Lysis Buffer (SIGMA). T cells were activated for 1.5 h with 5μl/ml of anti-CD28 and anti-CD49d in the presence or absence of 6.4μg/ml of a Gag pool of peptides in the presence of 0.5μg/ml Brefeldin A ...
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bioRxiv - Bioengineering 2019Quote: Cy3-(GT)6-SWCNTs and Cy3-(GT)30-SWCNTs were first filtered 3 times using 100kDa Amicon centrifuge filters (Millipore) to remove free Cy3-DNA from solution ...
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bioRxiv - Biochemistry 2019Quote: ... 3 mM KCl) and harvested in a PBS lysis buffer (PBS plus 6 uL/mL protease inhibitors cocktail (Sigma P8340), 0.6 mM PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 mL volumes of culture were transferred per well to 6-well tissue culture-treated polystyrene plates (Sigma CLS3506) and incubated at 23°C for 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Liposomes consisting of DPPC/egg yolk PC/egg yolk PG/cholesterol (6:3:1.2:0.95, DPPC from Avanti Polar Lipids, all other Sigma) with 0.6 μCi tracer [2-palmitoyl-1-14C]-dipalmitoyl phosphatidylcholine (14C-DPPC ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: hNSG and non-engrafted NSG mice (4-6 months old) were injected 3 mg/kg (i.p.) with lipopolysaccharide (LPS from E. coli; O55:B5, Sigma-Aldrich) or 0.9% saline solution ...