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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for 6 Chloro 7H pyrrolo 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...
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bioRxiv - Cancer Biology 2022Quote: ... Five thousand cNF-derived primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated opaque 96-well plates in SCM without IBMX with Forskolin and maintained at 37ºC under a 10% CO2 atmosphere for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates in SCM without IBMX ...
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bioRxiv - Biophysics 2022Quote: ... Concentrated Cav1.2(ΔC)/Cavβ3/Cavα2δ-1 sample was immediately incubated with gabapentin (final concentration 2 mg ml-1, 11.7 mM) (Sigma-Aldrich) on ice for four hours prior to cryo-EM sample preparation ...
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bioRxiv - Developmental Biology 2023Quote: ... unlysed embryos were cultured during 2 to 3 hours in 40 µL micro drops of TCM199 (Sigma-Aldrich, M4530) + 10% foetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were washed 3 times (5 minutes each) in wash buffer DPBS Tween containing 2% BSA (Sigma, A3311), and permeabilised at room temperature for 20 minutes in permeabilisation buffer 0.5% Triton-X in DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mg/ml of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich®, St Louis, MO, USA) was added and incubated at 37°C for 3h ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed at a 3:2 ratio with a crowding buffer [20% dextran (from Leuconostoc spp., Mr 450,000-650,000; Sigma 31392) and 1 mM DTT in 50 mM Tris-HCl pH 7.4] ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.4% Triton X-100 and 2% NHS PBS solution during 3 h and then coverslipped with Mowiol (Sigma). After confocal imaging ...
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bioRxiv - Cell Biology 2023Quote: ... Treatment of mice were performed with a single intraperitoneal injection of either vehicle (corn-oil) or 3 mg/kg 1,4-bis-[2-(3,5,-dichloropyridyloxy)] benzene (TCPOBOP, T1443, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: Culture medium was replaced with 100 µL medium containing 5 mg/mL filtered 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (#M5655, Sigma-Aldrich) and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich (St Louis, MO). Phalloidin-iFluor 594 was purchased from Abcam ...
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bioRxiv - Microbiology 2024Quote: ... after which the remaining supernatant (2 x 45ml) was collected and concentrated over a 3 kDa Amicon filter (Millipore). The residue was suspended in a volume of 900 µl ...
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bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) in de-ionised (DI ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) as counterstain.
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bioRxiv - Cell Biology 2022Quote: ... pLKO.1-puro-CMV-tGFP plasmids targeting SNX5 (5’-ACTATTACAATAGGATCAAAG-3’, 5’-CTGAGTATCTCGCTGTGTTTA-3’) and SNX6 (5’-AGTAAAGGATGTAGATGATTT-3’, 5’-GCCGAAACTTCCCAACAATTA-3’) (Sigma-Aldrich) were lentivirally transduced ...
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bioRxiv - Cancer Biology 2021Quote: ... MYC forward 5’-TGAGGAGGAACAACAAGATG-3’ and reverse 5’-ATCCAGACTCTGACCTTTTG-3’ and GAPDH forward 5’-CTTTTGCGTCGCCAG-3’ and reverse 5’-TTGATGGCAACAATATCCA-3’) (Sigma-Aldrich), the Radian SYBR Green Lo-ROX qPCR Kit (Alkali Scientific ...
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bioRxiv - Immunology 2022Quote: ... The sequences of forward and reverse primers for εGLT (fw: 5′-CTGTCCAGGAACCCGACAGA-3′; rev: 5′-TGCA GCAGCGGGTCAAG-3′) and γ1GLT (fw: 5’-CCAGGGCAGGGTCAGCA-3’; rev: 5’-GGTGCTCTTGGAGGAGGGT-3’) (Sigma-Aldrich) and their corresponding FAM-labelled MGB probes (εGLT ...
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bioRxiv - Genomics 2020Quote: ... HEK293T and U-2 OS cells were seeded on to poly-D-lysine (Cat. No. A-003-E, Sigma, St. Louis, USA) pre-coated 24-well plates at 24 h prior to transfection.
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bioRxiv - Genomics 2019Quote: ... 2×105 HEK293T cells were plated onto each well of a 25 ug/ml poly-D lysine (EMD Millipore, A-003-E) coated 24-well plate ...
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bioRxiv - Systems Biology 2019Quote: ... Each starter culture was then used to inoculate new cultures in YNB plus 2% D-glc (Sigma-Aldrich, G7528, St. Louis, MO), 0.2% D-glc ...
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bioRxiv - Systems Biology 2019Quote: ... This starter culture was then used to inoculate triplicate cultures in YNB plus 2% D-glc (Sigma-Aldrich, G7528, St. Louis, MO), 2% D-galUA ...
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bioRxiv - Genetics 2020Quote: ... blood glucose was measured using a glucometer from tail tip blood before and multiple times within 2 hours of intraperitoneal injection of 2g/kg D-(+)-Glucose (Sigma Aldrich # G7528).
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bioRxiv - Neuroscience 2020Quote: ... coated with poly-d-lysine (5 mg/50 ml distilled water for 2 hours, PDL; 135 kDa; Sigma-Aldrich, St. Louis, MO) or onto poly-d-lysine-coated BD BioCoatTM (BD Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl, 2 mM MgSO4, 0.2% n-dodecyl-β-D-maltoside [Nacalai Tesque, 14239-54] with protease inhibitor [Sigma-Aldrich, P8340]). After 20 min of incubation on ice ...
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Neuroscience 2022Quote: ... glia-containing samples were cultured with NBD supplemented with 2 μM of cytosine-β-D-arabinofuranoside (AraC, 500 μM in ultrapure water, C1768, Sigma Aldrich) to inhibit astrocyte and microglia proliferation ...
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bioRxiv - Neuroscience 2022Quote: ... Figure 3 – figure supplement 1C&D) or 0 mM CaCl2 together with 2 mM of the Ca2+ chelator EGTA-tetrasodium salt (Sigma, Germany, E8145) to buffer residual Ca2+ traces (Figure 3 – figure supplement 1A&B) ...
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bioRxiv - Neuroscience 2023Quote: ... OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα) (1:4000) (Supplementary Table 2) and then seeded on coverslips coated with poly-D-lysine (20 μg/ml) (Sigma-Aldrich; P0899) and incubated at 37°C and 10% CO2 in Sato medium ...
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bioRxiv - Biophysics 2023Quote: ... with a voxel-size of 0.636 × 0.636 × 2 μm) are recorded once before and 4 h after actin-depolymerization using 10 μM cytochalasin D (Sigma-Aldrich, St. Louis) using brightfield and second-harmonic generation imaging microscopy ...
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bioRxiv - Immunology 2023Quote: ... The lungs were harvested and grossly homogenized with scissors in HBSS containing 2 mg of collagenase D (Sigma Aldrich cat. no. 11088866001) and 0.25 mg of DNAse I (Sigma Aldrich cat ...
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bioRxiv - Physiology 2021Quote: ... The neurotoxin (6-hydroxydopamine) was purchased from Sigma-Aldrich (6-OHDA, H4381, St. Louis, MO).
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bioRxiv - Zoology 2019Quote: ... and pterin-6-carboxylic acid (pterin-6-COOH) were purchased from Sigma Aldrich (Munich, Germany). Isoxanthopterin and xanthopterin were obtained from Fluka (Buchs ...
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bioRxiv - Genomics 2021Quote: ... Antigen retrieval was done by microwaving for 6 minutes in citrate buffer pH 6 (Sigma). After quenching in 3% H2O2 in Methanol ...
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bioRxiv - Neuroscience 2022Quote: ... and a solution of 10 mg/mL 6-hydroxydopamine (6-OHDA; Sigma-Aldrich, H116-5MG), dissolved in saline buffer was injected ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were treated with 6-hydroxydopamine hydrochloride (6-OHDA, 15 µM, H4381, Sigma Aldrich) and METTL3/14 activators (100 nM or 1 µM) ...
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bioRxiv - Biophysics 2021Quote: ... Plexin A2 (R&D, Cat& MAB5486), Plexin D1 (R&D, Cat& AF4160), Plexin A4 (R&D, Cat& MAB5856) and FLAG (Sigma-Aldrich, Cat& F1804).
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bioRxiv - Biophysics 2021Quote: ... For the alkaline phosphatase fragmentation experiments D-(+)-Glucose (D-Glu; Sigma Aldrich, 20 mM) or L-(–)-Glucose (L-Glu ...
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bioRxiv - Biophysics 2022Quote: ... Me-α-D-Glc (≥ 99%) and D-xylose (≥ 99) was obtained from Sigma-Aldrich Chemie GmbH (Germany) ...
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bioRxiv - Neuroscience 2022Quote: ... 5.5mM d+ dextrose anhydrous (CAT# D16, Fisher) and 200mM d-sorbitol (CAT# S1876, Sigma)] and dissociated with 10 units of papain and 10μL of DNase in DMEM (37°C for 30 minutes) ...
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bioRxiv - Neuroscience 2023Quote: ... After 24h cells were treated with actinomycin D (Act-D, Catalog #A9415, Sigma, USA) at 10 μg/ml for 2 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% ascorbic acid was added to a sterile saline solution (NaCl, 0.9% w/v) and 6-OHDA HCl powder (6-hydroxydopamine hydrochloride from Sigma or 6-OHDA hydrobromide from Tocris) was then dissolved to produce a final 6-OHDA concentration of 4.44 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 4% 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS, Sigma)] ...
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bioRxiv - Neuroscience 2022Quote: ... 3 3’-diaminobenzidine tetrahydrochloride hydrate (DAB) (Sigma SIG-32750) in sodium acetate ...
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bioRxiv - Bioengineering 2021Quote: ... 3- [(3- Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, Sigma RES1300C), digitonin (Sigma D141) ...