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Citations for Millipore Sigma :
2101 - 2150 of 5691 citations for Resistant Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Annexin V-FITC apoptosis assay was carried out as described in manufacturer’s protocol (APOAF, Sigma. St. Louis, MO). After transfected with Npac RNAi or control RNAi plasmid in 6-well dishes and selected for 4 days ...
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bioRxiv - Plant Biology 2019Quote: ... The MUG assay buffer is same as that of the extraction buffer supplemented with 1 mM MUG (Sigma). The reaction was incubated at 37°C water bath for 20 min ...
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bioRxiv - Bioengineering 2019Quote: ... GAG quantities were determined using a modified dimethyl methylene blue (DMMB) assay.[72] Shark chondroitin sulfate (Sigma; C4348) was included as a standard ...
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Cancer Biology 2019Quote: ... The Duolink® in situ proximity ligation assay (PLA) was performed according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... Then the medium was collected and replaced with stimulation medium (basal assay medium containing 100 µM histamine, Sigma) for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... The assay was developed by addition of o-phenylenediamine dihydrochloride (OPD, Cat n. 34006, Sigma Aldrich, Gillingham, UK) for 10 minutes and reactions were stopped with 2 M H2SO4 ...
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bioRxiv - Bioengineering 2020Quote: ... GAG was quantified using a modified dimethyl methylene blue (DMMB) assay [42] with Shark chondroitin sulfate (Sigma, C4348) as a standard ...
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bioRxiv - Biochemistry 2021Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma-Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Biochemistry 2020Quote: GFAT’s amidohydrolysis activity was measured with a coupled enzymatic assay using bovine glutamate dehydrogenase (GDH, Sigma Aldrich G2626) in 96 well standard microplates (F-bottom ...
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bioRxiv - Plant Biology 2021Quote: ... The stress assays were performed on CM plates (Holliday 1974) supplemented with 750 µM calcufluor white (Sigma-Aldrich), 3 mM hydrogen peroxide (H2O2) ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed a final time and the assay was developed using the SigmaFast pNpp reagent (Sigma Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... and for flippase specific assays in the presence of an ATP regeneration system (0.5 mM Phosphoenolpyruvate (Sigma-Aldrich) and 100 U ml−1 pyruvate kinase (Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2021Quote: ... Measurement of IDH activity on the enriched mitochondrial fractions was conducted using an IDH activity assay (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cell pellets were thawed on ice before adding 50-100μl radio immunoprecipitation assay (RIPA) buffer (Sigma, Dorset, UK) with protease and phosphatase inhibitors and lysed for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed beads were resuspended in 25 μl of kinase assay buffer with 100 μM ATP (A7699, Sigma) supplemented with phosphatase inhibitors and incubated for 20 min in an Eppendorf Thermomixer at 30°C at maximal mixing velocity ...
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bioRxiv - Bioengineering 2022Quote: ... Finally, the activity assay of immobilised GalT consisted of 0.16 mM UDP Gal (Merck, 16mM GlcNAc (Sigma-Aldrich), 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: Cell viability was evaluated using the 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (Sigma-Aldrich), which measures mitochondrial activity in living cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell viability was evaluated by using the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay (Sigma). Non-treated cells were used as control ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with Bradford assay (Sigma B6916) on a nanophotometer (IMPLEN) ...
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bioRxiv - Microbiology 2022Quote: ... the capsid destabilizing compound PF74 was added for the assay at 10 μg/mL (Sigma, cat. SML0835-5).
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bioRxiv - Bioengineering 2019Quote: ... Miller.) Reactions were carried out in an RT assay buffer containing: 60 mM Tris (77-86-1, Sigma), 30 mM KCl (7447-40-7 ...
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bioRxiv - Cell Biology 2020Quote: ... and Click-iT® EdU (5-ethynyl-2’deoxyuridine) Assay (BCK-EDU488, baseclick GmbH, Merck/Sigma-Aldrich, UK), respectively ...
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bioRxiv - Neuroscience 2020Quote: qPCR (q-PCR) primers were designed using Universal probe library Assay Design Center and were purchased from Sigma. Transcriptor High Fidelity cDNA Synthesis Kit (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... Cleavage efficiency of the selected sgRNAs was tested using cel-1 assay in mouse neuroblastema (N2a) cells (SIGMA) with the primer pairs listed in Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... EC extracts were immunoprecipitated by incubation with 10 µg Rac1 assay reagent (PAK-1 PBD magnetic beads, Sigma) for 45 minutes at 4°C with gentle agitation ...
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bioRxiv - Plant Biology 2021Quote: ... All chemical reagents used for the chemical characterization and biochemical assays were purchased from Sigma-Aldrich (Arklow, Ireland) and Bio-Rad (Watford ...
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bioRxiv - Cancer Biology 2021Quote: ... The HRP reaction was initiated by addition of 3,3’,5,5’-tetramethylbenzidine enzyme-linked immunosorbent assay substrate solution (Sigma) and terminated after 30 min by adding H2SO4 (2M ...
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bioRxiv - Cell Biology 2021Quote: ... viability was determined with the MTT assay using Thiazolyl Blue Tetrazolium Bromide (MTT) reagent purchased from Sigma-Aldrich. Neurons were cultured in 96-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... 97062]) and then overlaid with 175μL of mitochondrial assay solution supplemented with 10mM sodium pyruvate (Sigma-Aldrich, P5280), 1mM malate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with mitochondrial assay solution (220mM mannitol [Sigma-Aldrich, M4125], 70mM sucrose [Sigma-Aldrich, S7903] ...
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bioRxiv - Molecular Biology 2022Quote: ... The GTPase activity was also measured using the malachite-green assay as per the manufacturer’s protocol (Sigma-MAK113). The binding of GTP to recombinant proteins was checked as described in (Rohn ...
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bioRxiv - Microbiology 2022Quote: ... and the data normalized to total protein content that was determined by the bicinchoninic acid (BCA) assay (Sigma).
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bioRxiv - Physiology 2023Quote: Plasma FG and TRIG were measured using a sequential color endpoint assay (Sigma-Aldrich, reagents F6428 and T2449) modified for small plasma volumes (5 μl ...
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bioRxiv - Neuroscience 2022Quote: ... BV2 cells were seeded for the assay at density of 10 000 cells/well on PDL (Sigma, P0899) coated 96-well plates and iMGLs as for cytotox assay ...
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bioRxiv - Plant Biology 2022Quote: ... water was replaced by 150 μL of assay solution containing 2 μg/mL horseradish peroxidase (Sigma-Aldrich, Germany) and 100 μM luminol (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Microbiology 2022Quote: ... Assay was conducted according to the manufacturer’s instructions and recombinant L-Lactic Dehydrogenase from porcine heart (Sigma-Aldrich) was used to generate a standard curve ...
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bioRxiv - Microbiology 2023Quote: ... cells lysates were performed in radioimmunoprecipitation assay (RIPA) lysis buffer (Sigma-aldrich Humeau, La Chapelle-Sur-Erdre, France) at 4 °C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IL-1β and TNF-α using indirect sandwich enzyme-linked immunosorbent assay (ELISA) (Sigma-Aldrich; #RAB0308 and #RAB0477) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: Cell-free assays were set up manually in white flat bottom 96-well plates (BRANDplates®;Sigma-Aldrich) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Aldicarb assays were performed on day 1 adult hermaphrodites on NGM plates containing 1 mM Aldicarb (Millipore-Sigma). Arrays were created by gonadal microinjection ...
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bioRxiv - Neuroscience 2023Quote: ... Aldicarb assays were performed on day 1 adult hermaphrodites on NGM plates containing 1 mM Aldicarb (Millipore-Sigma). Arrays were created by gonadal microinjection ...
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bioRxiv - Microbiology 2023Quote: The MILLIPLEX Ovine Cytokine/Chemokine Panel 1 Premixed 14-plex - Immunology Multiplex Assay (Millipore Sigma, SCYT-91K-PX14) was used to quantify 14 cytokines in sheep serum samples following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... radioimmunoprecipitation assay (RIPA) buffer and 1,1,1,3,3,3 hexafluoro-2-propanol (HFIP) were purchased from Sigma Aldrich (St. Louis, USA). Cell culture dishes ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were conducted using FP buffer [SEC buffer plus 0.1 mg mL-1 bovine IgG (Sigma-Aldrich) and 0.5 mM Thesit (Sigma-Aldrich)] Binding affinities were determined using SOLVER ...
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bioRxiv - Cell Biology 2023Quote: ... and the proximity ligation assay was performed according to the DUOLINK manufacturer’s guideline for mammalian cells (Sigma-Aldrich). DNA was stained with DAPI-containing mounting medium (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... and mixed 1:1 with a 2X assay solution of 40 U/mL lactate dehydrogenase (Sigma-Aldrich L3916), 1 M Tris (pH 9.8) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured after 72hrs (or at the indicated time point) using the MTT assay (Sigma M2128), with 500ug/mL salt diluted in complete medium and incubated at 37°C for 3 hrs ...
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bioRxiv - Genomics 2023Quote: Luminex assays were performed using the Milliplex Human Cytokine/Chemokine Magnetic Bead Panel (HCYTOMAG-60K-41, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined after 72 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (#M5655, Sigma) following manufacturer’s instructions ...