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Citations for Millipore Sigma :
2101 - 2150 of 4323 citations for Recombinant Human IL12B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... human leukemia inhibitory factor (hLIF, 10 ng/ml, Millipore), SB431542 (2 µM) ...
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bioRxiv - Immunology 2024Quote: ... with 5% v/v heat-inactivated human serum (Sigma), 1% v/v Glutamax (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5% human AB serum (Millipore Sigma, H4522), 2 mM L-glutamine (Gibco ...
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bioRxiv - Biochemistry 2019Quote: 25 µl of 100 µM recombinant purified GST Pat1-NC was bound to 30 µl GST-slurry (Sigma), and one aliquot of GST-beads was included without added Pat1-NC as negative control ...
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bioRxiv - Immunology 2019Quote: ... supplemented with cytokine cocktails aimed at proliferation (50 ng/ml murine recombinant thrombopoietin (TPO, Sigma-Aldrich SRP3236-10UG) and 100ng/ml Stem Cell Factor (SCF) ...
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bioRxiv - Immunology 2019Quote: ... NMRI mice were injected subcutaneously at two different locations with 100µg of the recombinant Thio-EmACT resuspended in 100µl Freund Incomplete adjuvant (Sigma). The double injections were repeated four weeks later to boost the mice anti-EmACT response ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Biochemistry 2022Quote: ... and ∼1 μg recombinant GST or GST-SMN in freshly made 25 mM bis tris propane (Sigma B6755) pH 6.8 buffer with 1 M NaCl and 0.05% NP-40 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells that were cultured in 0.5 % FBS were stimulated with hEGF (recombinant EGF) (Sigma Alrdich, Catalog No: E9644) in concentrations (10 and 30 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Microbiology 2022Quote: ... Assay was conducted according to the manufacturer’s instructions and recombinant L-Lactic Dehydrogenase from porcine heart (Sigma-Aldrich) was used to generate a standard curve ...
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Cell Biology 2023Quote: ... either coated with BSA or recombinant Laminin332 (BioLamina LN332-0502, diluted in Dulbecco’s Phosphate Buffered Saline, Sigma, D8662) and left to adhere overnight ...
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bioRxiv - Biochemistry 2023Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Supplemented IVM media consisted of MediCult IVM media (Origio) supplemented with 75 mIU/mL of recombinant FSH (Millipore), 100 mIU/mL recombinant hCG (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... with 50 μg of either recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... The recombinant APC/C complex was eluted with the wash buffer supplemented with 2.5 mM desthiobiotin (Sigma-Aldrich). Before loading the elution fractions onto a 6 mL Resource Q anion-exchange column (Cytiva) ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ku80 (CST, #2180, 1:400), anti-human nuclear antigen (Novus NBP2-34342, 1:200) and anti-human-specific Albumin (Sigma, A3293, 1:200). Species-specific secondary antibodies conjugated with Alexa Fluor 555 ...
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bioRxiv - Bioengineering 2020Quote: ... of 0.3 with Edit-R lentiviral mKate2-tagged, constitutively expressed Cas9 nuclease under the hEF1α promoter (Dharmacon, CAS11229) in transduction medium containing 5 μg/mL polybrene (EMD Millipore, TR-1003-G). Complete growth medium was added to the transduced cells at a 3:1 dilution after 5 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... human insulin to 10µg/mL (Sigma Aldrich, St. Louis, MO), human epidermal growth factor to 20ng/mL (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 0.005 mg/ml human plasma fibronectin (EMD Millipore) at 37°C for 30 minutes ...