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Citations for Millipore Sigma :
2101 - 2150 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... followed by FITC-labeled goat anti-mouse IgG antibody (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Cancer Biology 2021Quote: ... then the cells were labeled with a FITC-conjugated anti-CD15 antibody (Millipore, CBL144F) and a phycoerythrin (PE)-conjugated anti-CD133 antibody (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... and were labeled with 5 mM of carboxy-fluorescein diacetate succinimidyl ester (CFSE) (Sigma) in PBS for 15 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... and then treated with 1 mM unlabeled or U-13C-labeled sodium butyrate (Sigma). Cell monolayers were rinsed with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cholinergic neurons were labeled with a mouse monoclonal antibody to ChAT (Sigma, AMAb 91130) and goat anti-mouse 647 secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cortactin staining was performed with dye-labeled Anti-cortactin (05-180-AF647, Millipore) and therefore the secondary antibody treatment was skipped.
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bioRxiv - Immunology 2020Quote: ... followed by FITC-labeled goat anti-mouse IgG antibody (Sigma-Aldrich, St. Louis, MO). Mean fluorescence intensity (MFI ...
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bioRxiv - Molecular Biology 2022Quote: 15N survivin was produced in M9 medium containing 15N labeled ammonium chloride (Sigma-Aldrich). Expression was induced with 0.5 mM IPTG and incubated for 20-22 h at 15 °C ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in an appropriate fluorescently labeled secondary antibodies (Sigma-Aldrich, USA). The sections were then mounted using Vectashield hard set mounting medium (Vector Laboratories ...
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bioRxiv - Immunology 2022Quote: We labeled approximately 1 million CAR T cells with PKH67 green fluorescent dye (Sigma) and 1 million NALM-6 cells with PKH26 red fluorescent dye (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Tubulin and centrosomes were labeled using anti-tubulin monoclonal DM1-alpha (1:500, Sigma) or anti-pericentrin (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... isolated EVs were labeled according to the manufacturer’s instructions (MIDI6-1KT, Sigma, Burlington, MA). Excess PKH was removed by centrifugation at 190,000g for 2 hours with a 0.971M sucrose cushion (SW55Ti rotor ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: Myristoylated GFP (myr::GFP) was labeled with 1:500 mouse anti-GFP (Sigma-Aldrich, Cat#G6539 ...
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bioRxiv - Cancer Biology 2023Quote: ... GFP-labeled GBM cells were detached from the culture vessel with Accutase™ (Sigma) and re-suspended in media ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were subsequently labeled with DAPI and mounted using Fluorescent Mounting Media (EMD Millipore) to prevent fading.
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bioRxiv - Microbiology 2023Quote: ... resuspended in 1x PBS and labeled with 0.1 mg/ml fluorescein isothiocyanate (FITC) (Sigma) at 4°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... in TBS) and then labeled with anti-laminin antibody (Millipore Sigma L9393, 1:100) to demarcate myofiber perimeter diluted in blocking buffer and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were pulse-labeled with BrdU (10 μM, Cat# B9285, Millipore Sigma, 30 min) before or after treatment under indicated conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... nuclei were fluorescently labeled by incubation with 1 µg/µl Hoechst 33342 (Sigma-Aldrich) in PBS for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were labeled with a photo-reactive and clickable cholesterol analog (pacChol) (Sigma-Aldrich) with a final concentration of 6 µM for 30 min at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... while those labeled with ATTO565 at the 5′ end was synthesized by Sigma Aldrich. All readout probes underwent purification by high-performance liquid chromatography (HPLC).
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bioRxiv - Molecular Biology 2023Quote: ... while those labeled with ATTO565 at the 5’ end was synthesized by Sigma Aldrich. All readout probes underwent purification through High-Performance Liquid Chromatography (HPLC).
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bioRxiv - Cancer Biology 2023Quote: ... Digoxigenin labeled probes were visualized using two layers of antibodies: rabbit anti digoxin (Sigma) followed by goat anti rabbit IgG Cy5.5 (Linaris) ...
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bioRxiv - Plant Biology 2023Quote: ... 8 pmol M13-labeled forward primer and 32 pmol reverse primer (Sigma Life Science), standard Taq buffer (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... containing internal following standards: C13 and N15 labeled amino acids standard mix (Sigma, 767964) (1:1500) ...
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bioRxiv - Neuroscience 2024Quote: ... digoxygenin (DIG)-labeled antisense probes were prepared using the Roche DIG labeling kit (Sigma Cat # 11277073910 and T7 (Sigma Cat # 10881767001 ...
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 (human embryonic kidney) and U2OS (human bone osteosarcoma) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10 % FBS ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T) and human fibroblast cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich), supplemented with fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: The following antibodies were used: mouse anti APC (Millipore OP80-100UG, 1:100), rabbit anti B2m (abcam ab75853-100ul ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: anti-APC mouse monoclonal antibody (1:200; Merck Millipore, Burlington, MA, USA), anti-GFAP-488 mouse monoclonal antibody (1:300 ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitation (IP) of Flag-tagged ULK1 construct was performed using EZviewTM Red ANTI-FLAG ® M2 Affinity Gel (Sigma-Aldrich, F2426) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein lysates were diluted to 1 ml with CoIP Lysis Buffer and the 3xFLAG-tagged bait was immunoprecipitated by incubation with 20 μl of anti-FLAG M2 Magnetic Beads (Sigma M8823) for 2 hrs at 4 °C on a tube rotator ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Molecular Biology 2020Quote: Primer sequences for markers were obtained from the UCSC genome browser and standard HEX-tagged primers (Sigma-Aldrich, Supplementary Table 2) were used to assess the flanking haplotypes of MMP20 ...
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bioRxiv - Cell Biology 2022Quote: ... C-terminal FLAG-tagged OCT4 and various SOX TFs were generated by subcloning cDNAs into pFLAG-CMV5a mammalian expression vector (Sigma-Aldrich). For stable expression of mCherry and SOX15 in OEC-2 cells ...
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bioRxiv - Microbiology 2021Quote: ... The HS and CS samples were analyzed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) and quantified by inclusion of [13C6]aniline-tagged standard HS disaccharides (Sigma-Aldrich), as described (Lawrence et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The blot was stripped and then reprobed for FLAG-tagged receptor using anti-FLAG antibody (1:2000, Sigma, Cat. no. A8592). Data were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Systems Biology 2020Quote: Mammalian expression vectors encoding Flag-epitope tagged full-length Csk and eGFP-tagged Ptpn18 WT & Y/F mutant were transfected into 293T cells using 5 ug/mL PEI (Polyethylenimine, Sigma Aldrich) in Opti-MEM (GIBCO) ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear extracts from HEK 293 cells expressing either FLAG-tagged CXXC or PHD constructs were incubated with anti-FLAG M2 beads (Sigma Aldrich) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... Degradation of the AID-tagged RAD21 was induced by the addition of 500 μM (final concentration) indole-3-acetic acid (IAA/auxin; Sigma Aldrich) during media change 24 hours prior to imaging if applicable.
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bioRxiv - Cell Biology 2020Quote: Purification of mammalian Cavin1 was performed by Transfecting GFP-tagged Cavin1 using polyethylenimine (PEI) transfection reagent (Sigma-Aldrich Cat. No. 408727) with 1:4 w/w ratio (DNA:PEI ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of lysates of hTERT-RPE1 cells stably expressing HA-tagged WDR60 or WDR34 using anti-HA agarose beads (Sigma-Aldrich). Lysis buffer containing 50 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... cell surface HS level was examined after staining with anti-HS antibody (10E4, amsbio) at 5 μg/ml and FLAG-tagged enzymes were detected by using anti-FLAG® M2 antibody (Sigma), at 2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Plant Biology 2022Quote: ... resolved by SDS-PAGE and immunoblotted for the presence of the HA- tagged effector using α-HA antibody (H9658; Sigma-Aldrich) and α-HA-HRP (3F10 ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...
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bioRxiv - Biochemistry 2023Quote: Hexahistidine tagged hMDC1 BRCT (residues: 1891 – 2089) was cloned into pET47b vector and expressed in Rosetta™ 2(DE3) cell lines (Novagen). Cells in 1L Luria Broth (LB ...