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Citations for Millipore Sigma :
2101 - 2150 of 10000+ citations for 6 Bromo 3 4 dihydro 2H 1 benzothiin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Synaptosomes were prepared from the striatum or cortex of VGLUT1venus or DAT-Cre eGFP-expressing mice by homogenization in 1ml of ice-cold isosmolar buffer (0.32M sucrose, 4 mM HEPES pH7.4, protease inhibitor cocktail Set 3 EDTA-free (EMD Millipore Corp.)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Percentage of GFP positive cell population was measured at day 3 or day 4 as initial time point using a Guava Easycyte HT instrument (Millipore). GFP% (mCherry% ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Physiology 2021Quote: ... was incubated for 2-3 h at 4 °C with 30 µl of FLAG-conjugated beads (anti-DYDDDDDK beads, Sigma) or empty beads (ThermoScientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck ...
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bioRxiv - Plant Biology 2019Quote: ... The total proteins were extracted by using lysis buffer and then incubated for 3 h at 4°C with 20 ul of Anti-HA Affinity Gel (Millipore). The immunoprecipitates were washed five times with lysis buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... after 72 hours of drug treatment, 20μl of a 5mg/mL 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (M2128-MTT; Sigma-Aldrich, USA) solution was added to each of the wells ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Physiology 2021Quote: ... The preparation was bathed in standard locust saline (in mM: 147 NaCl, 10 KCl, 4 CaCl2, 3 NaOH and 10 HEPES buffer; pH = 7.2; chemicals from Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... 2-3 mg of sNASP-FLAG-myc and sNASP-Strep-HA extracts were incubated for 3 hours at 4 °C degrees with 50 μl of anti-Flag M2 (A2220, Sigma) or Anti-TwinStrep Mag-Strep beads (2-4090-002 ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing eluted BfNFeoAB were concentrated at 4°C using a 15 mL Amicon 3 kDa molecular-weight cutoff (MWCO) spin concentrator (Millipore). Concentrated protein was then further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 1 hour in 3% BSA-TBS and incubated overnight at 4°C with the following primary antibodies: anti-phosphotyrosine 4G10 (Millipore), anti-phosphorylated SYK (pY525/526 ...
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bioRxiv - Molecular Biology 2022Quote: ... were diluted in PBS together with 3 μg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma) and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418; Sigma). The culture was incubated with shaking (120 rpm ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 minutes and washed 3-4 times in PBS before being mounted in Mowiol mounting media (Millipore, 475904-M).
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and 3-4 min at room temperature with a pancreatin-trypsin mixture (2.5mg/ml pancreatin [P3292; Sigma Aldrich] and 22,5mg/ml trypsin in Thyrode’s solution pH 7.4) ...
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bioRxiv - Neuroscience 2022Quote: ... or DMSO 0.1% as vehicle was evaluated using a MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) assay as described in (Sanz et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was filtered at 300 × g for 3 minutes at 4°C through a 0.45-μm 96-well filter plate (Millipore, MSHVN4550) to remove protein aggregates ...
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2023Quote: iLPC organoids formed after 48 hours post-seeding were fixed for 3 hours in 4% paraformaldehyde (PFA) in phosphate buffered saline (PBS) (Sigma), before a double-embedding process in 1.5% agarose gel then paraffin as described previously [13 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro excision of Nr3c1 exon 3 was achieved by treating primary myoblasts with 4-OH-tamoxifen (Sigma Aldrich, H7904).
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bioRxiv - Developmental Biology 2023Quote: ... in PBS at +4⁰C in the shaker and then 3-4 min with a pancreatin-trypsin (2.5 mg/ml pancreatin [P3292; Sigma Aldrich] and 22.5 mg/ml trypsin dissolved in Thyrode’s solution pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: Cells were fixed in 4% paraformaldehyde and permeabilized/blocked using PBS with 3% goat or donkey serum and 0.2% triton X-100 (Sigma Aldrich). Cells were incubated at 4°C overnight with primary antibodies against the following targets ...
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bioRxiv - Plant Biology 2023Quote: Samples were incubated with 20 µL of IS solution (155 µmol/L forchlorfenuron, 25.5 µmol/L 4-chlorphenylacetic acid, 5.16 mmol/L indole-3-acetic-2,2-d2 acid, Sigma-Aldrich, Steinheim, Germany) and diluted with an acetonitrile/methanol + 1% formic acid solution (4:1 ...
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bioRxiv - Bioengineering 2023Quote: ... tricaine-anesthetized 3 or 4 days-post fertilization (dpf) larvae were mounted in 2% low-melting point agarose (Sigma-Aldrich) on a 35 mm glass bottom dish (#1.5H Glass ...
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bioRxiv - Cell Biology 2019Quote: ... Co-immunoprecipitation was performed by incubating cell lysate with with anti-MYC conjugated agarose beads for one hour at 4°C (Sigma-Aldrich, St. Louis, MO). Phos-tag analysis was performed using phosphoproteins obtained by TCA precipitation and separated with phos-tag acrylamide gels as previously described (Fujifilm Wako Pure Chemical ...
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bioRxiv - Genomics 2020Quote: ... We followed the protocol as described previously [6] with one alteration: instead of using one paperdisc (Whatman qualitative filter paper, WHA1001070, SIGMA-ALDRICH CO LTD) per extraction ...
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bioRxiv - Systems Biology 2023Quote: ... 6 M Urea and 1% Sodium Dodecyl Sulphate supplemented with 100mM phenylmethylsulfonyl fluoride and 1% phosphatase inhibitor cocktail 3 (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2020Quote: ... with a manufacturer-provided polymer FC720 and surface-salinized in a vacuum desiccator overnight with 20 µl Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma-Aldrich, USA). The microfluidic chips were manufactured via soft-lithography ...
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bioRxiv - Biophysics 2023Quote: ... the master SU-8 2050 photoresist was treated with trichloro(1H,1H,2H,2H-perfluorooctyl)silane (448931, Sigma-Aldrich, Saint Louis, MO) in a desiccator for overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Pregnant females were injected intraperitoneally at E12.5 with 75 μg/g 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich #H6278) dissolved in corn oil ...
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bioRxiv - Neuroscience 2021Quote: ... The experimental mice were injected intraperitoneally with 4-Hydroxytamoxifen (4-OHT) (Sigma Aldrich; Cat.#:H6278-50mg; 75mg/kg) once per day during 4 consecutive days ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 mL of Working Hanks 4 (WH4; 1X HBSS/4 g/L total glucose/1X antibiotic-antimycotic (Sigma)) was added ...
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bioRxiv - Bioengineering 2022Quote: ... The PEG pre-polymer phase comprised 27.5% w/v 5kDa 4-arm PEG acrylate (Advanced BioChemicals) with 4% w/v lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, Sigma) in phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: U2OS cells were grown on 4 well glass slides (Millicell EZ SLIDE 4 well glass, Millipore Sigma PEZGS0416). After washing three times in 1× PBS (pH 7.4) ...
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bioRxiv - Developmental Biology 2021Quote: ... Pregnant females were injected intraperitoneally (i.p.) with 10 mg/kg 4-hydroxytamoxifen (4-OHT, Millipore-Sigma cat #H6278) dissolved in corn oil (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... Pregnant females were injected intraperitoneally (i.p.) with 10 mg/kg 4-hydroxytamoxifen (4-OHT, Millipore-Sigma cat #H6278) dissolved in corn oil (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... in addition to the corresponding drug treatment: either 4-hydroxytamoxifen (4-OHT; Sigma Aldrich; stock concentration: 13 mM) or its vehicle ...
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bioRxiv - Physiology 2022Quote: ... The soluble material was incubated for 4 hours at 4°C with anti-Flag M2 magnetic beads (Sigma). Beads was washed with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... ChK2 inhibition was carried out by using 2-(4-(4-Chlorophenoxy) phenyl)-1H-benzimidazole-5-carboxamide hydrate (Sigma) at 0.2 mM concentration.
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bioRxiv - Biochemistry 2021Quote: ... The pTriEx-4 Ek/LIC hTG4 vector was constructed using pTriEx™-4 Ek/LIC Vector Kit (Novagen) based on the manufacturer instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... Setd2flox/flox MEFs expressing ER-Cre were treated with 3μM (Z)-4-hydroxytamoxifen (4-OHT, Millipore Sigma, H7904) or vehicle (Ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... The PEG pre-polymer phase comprised 27.5% w/v 5 kDa 4-arm PEG acrylate (Advanced BioChemicals) with 4% w/v lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, Sigma) in phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... supplemented with protease inhibitors (4 μM PMSF, 20 μg/ml Pepstatin A, 4 μg/ml Leupeptin) (Sigma Aldrich), 5 μg/mL DNase and 10 μg/ml RNAse (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and fixed in FACS Buffer containing 2 % FBS and 4 % para-formadehyde (Sigma-Aldrich #95-30525-89-4). Zombie Aqua Fixable viability dye (Biolegend # 423101 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then exchanged into the NMR buffer using Amicon Ultra-4 centrifugal concentrators (4 mL; Millipore Sigma) with a 3-kDa molecular cutoff to a final volume of ∼250 μL ...