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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Myelin Basic Protein (MBP; Millipore) was used as a positive control ...
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bioRxiv - Neuroscience 2022Quote: ... 2.0 Protein Detection System (Millipore). The following antibodies were used ...
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bioRxiv - Physiology 2022Quote: Magnetic protein G beads (Sigma) were used per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Protein Detection System (EMD Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Heterochromatin protein (Hp1) (MAB3866, Millipore), or Dnmt1 (SantaCruz ...
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bioRxiv - Microbiology 2023Quote: ... or Sepharose protein G (Sigma) were added and further incubated with shaking for 3 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Protein analytical standard solution (Sigma) was used for plotting the standard chart for Stoke’s radius determination.
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bioRxiv - Immunology 2023Quote: ... Protein was trypsin (Sigma Aldrich) digested at 37°C for 18 hours ...
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bioRxiv - Immunology 2023Quote: ... Protein was trypsin (Sigma Aldrich) digested at 37°C for 18 hours ...
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bioRxiv - Cell Biology 2023Quote: ... with protein inhibitor cocktail (Sigma) and subsequently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... 1% penicillin/streptomycin and ITS supplement (5 μg/ml insulin, 5 μg/ml transferrin, 5 ng/ml sodium selenite) (GenDEPOT, Houston, TX; Sigma-Aldrich, St. Louis, MO) and cell passages 6-9 were utilized for all experiments ...
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bioRxiv - Plant Biology 2019Quote: ... The antigen–antibody complexes were visualized using nitrotetrazolium blue chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) buffer (Sigma-Aldrich, St. Louis, Missouri, USA) under standard conditions ...
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bioRxiv - Neuroscience 2021Quote: ... The blot was blocked in 5% non-fat milk in TBS-T and probed with primary antibodies mouse monoclonal anti-parvalbumin (Sigma-Aldrich Cat# P3088, 1:2000) and mouse monoclonal anti-β-actin (Sigma-Aldrich Cat# A5441 ...
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bioRxiv - Immunology 2022Quote: ... membranes were incubated with PBS-T or TBS-T complemented with 5 % BSA and primary antibodies specific for β-actin (Sigma-Aldrich, A5441, 1:2000), calnexin (Enzo Life science ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.4) for 1 h at room temperature and were subsequently incubated with primary antibodies rabbit anti-claudin-5 (Sigma-Aldrich, cat# SAB4502981, AB_10753223, 1:300), mouse anti-claudin-5 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed and incubated with the appropriate secondary antibody in 5% non-fat dry milk in PBST for 2h at RT and revealed in Chemiluminescent HRP Substrate (Sigma Aldrich, Saint Quentin Fallavier, France).
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bioRxiv - Immunology 2019Quote: ... and placed in 1X PBS containing 5 mM EDTA and 5 mM DTT (Sigma-Aldrich). Tissues from 3-4 pups (per infection group ...
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bioRxiv - Microbiology 2020Quote: ... 5-10 ml of schizonts at <5% parasitaemia were lysed using 0.1% saponin/PBS (Sigma) for 10 min (room temperature) ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was quenched by addition of 5 μL 5% (v/v) hydroxylamine (Sigma Aldrich), followed by a 15 minutes incubation step ...
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bioRxiv - Molecular Biology 2022Quote: ... Oligonucleotides 5’-GAAAAAAAAAATATACGCTAAGATTTTTGG-3’ and 5’-ATGACTAAACCCCCCCTCC-3’ synthesized and HPLC-purified by Sigma-Aldrich were used ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-chloro-2’-deoxyuridine (CIdU) and 5-lodo-2’-deoxyuridine (ldU) were obtained from Sigma.
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bioRxiv - Genetics 2021Quote: ... protease inhibitors (5 μM E64, 10 μM Bestatin, 5 μM Pepstatin, 48 hours (Merck Millipore)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... injections were resuspended in a 5% EtOH /5% Kolliphor-EL (Sigma-Aldrich Cat. No. C5135) / 90% water for injections vehicle ...
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bioRxiv - Bioengineering 2023Quote: ... Tendons (n = 5/group/time) were then digested with proteinase-K (5 mg/ml) (Sigma) for 18 hours and stored at -20C until further assays could be performed ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 5-fluorouracil (5-FU) and crystal violet from Sigma-Aldrich (St. Louis, MO, USA). They were dissolved in dimethylsulfoxide (DMSO ...
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bioRxiv - Neuroscience 2023Quote: Subcutaneous injections of 5-Chloro-2’-deoxyuridine (CldU) or 5-Iodo-2’deoxyurdine (IdU) (Sigma) were administered (50μg/g dissolved in saline ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-diphenyl-2H-tetrazolium bromide solution (MTT) (Sigma-Aldrich, A2231, 5 mg/mL in PBS) to each well ...
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bioRxiv - Physiology 2024Quote: ... CDH1 Forward 5′-TTACTGCCCCCAGAGGATGA-3′ and Reverse 5′-TGCAACGTCGTTACGAGTCA-3′;) were purchased from Sigma-Aldrich. mRNA expression was determined using the comparative 2-ΔΔCt method and normalized to the mRNA expression level of endogenous references (PPIA ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µl of the supernatant was incubated with 5 µg of amyloglucosidase (Sigma- Aldrich A1602) with 50 mM Na acetate for 30 minutes at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with antibodies against selected proteins overnight at 4°C: Anti-β-Tubulin III (β-Tub III) (Sigma, T8660, mouse, 1:2000); anti-p-Tau (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The loading consistency was verified utilizing the house-keeping proteins (Rab11 and β-Actin) by stripping and re-probing blots with monoclonal β-actin primary antibody (Sigma, St Louis, MO) following the similar procedure as described earlier.25 ...
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bioRxiv - Genetics 2022Quote: ... the membrane was probed with the primary antibody overnight at 4°C (rabbit anti-beta galactosidase antibody, 15518-1-AP Protein Tech, dilution 1/1000: mouse anti-alpha-tubulin, T6199 Sigma-Aldrich, dilution 1/10 000) and then 1 hour at room temperature with the HRP linked secondary antibody (HRP-linked anti-rabbit IgG ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... Even protein loading and successful protein transfer was confirmed using Ponceau S stain (Sigma Aldrich) prior to probing with primary antibodies (Table S2) ...
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bioRxiv - Cell Biology 2022Quote: ... for total protein loading quantification before proteins were transferred to PVDF membrane (Millipore, Cat. # IPVH00010). The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... with protein concentrations normalized to total protein levels in lysate (BCA assay; Sigma-Aldrich®).
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bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was estimated using the Bio-Rad Protein Assay Dye Reagent (Sigma). 10 µl of liver lysate was transferred to 96-well plate and ALT was measured using a microplate-based ALT activity assay kit (Pointe Scientific ...
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bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... the concentration of EV protein was quantified by using a BCA Protein Assay Kit (Novagen). For BMDC stimulation by EVs ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein-protein interaction was identified using a commercially available PLA kit (Duolink; Sigma-Aldrich) as described before 34 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore) or GFP-Trap®_A beads (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Total proteins extraction from hPSCs was performed in ice-cold RIPA protein extraction buffer (Sigma) supplemented with protease inhibitors (Protease inhibitor cocktail set I ...
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bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
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bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...