Labshake search
Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... A total of 0.1 mg mucin per dot were immobilized by dot blotting onto an immobilon-P PVDF membranes (Immobilon P membranes, 0.45 μm, Millipore, Billerica, MA). RiGH98 was added to one dot to 1.5 μM in 50 μL and incubated for 1 h and 4 h at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Each spinal cord slice was adhered to a small piece of filter membrane (Whatman Nuclepore Track-Etched Membrane, Sigma-Aldrich, WHA110657) for convenient handling ...
-
bioRxiv - Developmental Biology 2023Quote: ... 95°C and run on 10% SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham Protran Western blotting membranes – Sigma Aldrich) to validate protein quality and specificity.
-
bioRxiv - Cell Biology 2023Quote: ... DNA was crosslinked to the membrane with a UVA Stratalinker 2400 and membranes were then soaked in PerfectHyb Plus (Sigma Aldrich) for 20 minutes at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were loaded in 7.5% polyacrylamide resolving gel and electroblotted into a polyvinylidene fluoride membrane (Immobilon Transfer membrane, IPVH00010, Merck Millipore, Cork, Irlande). Blots were blocked in phosphate buffer saline (PBS ...
-
bioRxiv - Developmental Biology 2024Quote: ... protein lysates were separated by size via SDS-PAGE and transferred to a nitrocellulose membrane. Membranes were incubated over night at 4 °C with Trim71 (Worringer et al. 2014) or Vinculin (Sigma-Aldrich) antibodies (both 1:1000 in 5% milk powder in TBST ...
-
bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was used to assay for ANP levels using a competitive enzyme Immunoassay kit from Sigma-Aldrich (Catalog Number RAB0385). The levels of ANP were normalized for the amount of protein ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were treated for 2 hours by the Autophagy Reagent A (rapamycin containing) from the FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (Millipore, FCCH100171), which have been diluted at 1:50 as per the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... the cells were lysed and the ATP content was measured by an ATP assay kit per manufacturer’s instruction (Millipore Sigma, n = 3).
-
bioRxiv - Bioengineering 2021Quote: ... after 48 hours of incubation at 37°C were performed according to the manufacturer’s instruction (Malachite Green Phosphate Assay Kit, Sigma-Aldrich, The Netherlands). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... Sulfate was determined via the barium precipitation method using the Sulfate Assay Kit following manufacturer’s instructions (Sigma Aldrich, Saint Louis, MI, USA). Sample measurements were carried out in technical triplicates.
-
bioRxiv - Immunology 2021Quote: ... Concentrations were determined in duplicate by fluorescent-coded magnetic beads (MagPlex-C; MILLIPLEX. MAP Kit, Canine Cytokine Magnetic Bead Panel, 96-Well Plate Assay, CCYTO-90K, Millipore, Billerica, MA), based on the Luminex xMAP technology (Luminex 200 ...
-
bioRxiv - Cell Biology 2020Quote: ... in serum was analyzed by measuring 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc) using Ketone body assay kit (Sigma, No. MAK134) according to manufacturer’s instruction ...
-
bioRxiv - Systems Biology 2020Quote: Plasma aliquots of blood collected from the tail vein after 4-hour fasting were used to measure the concentration of glucose and triglycerides by enzymatic colorimetric assays using commercially available kits (Sigma-Aldrich, USA) according to manufacturer’s instruction [17] ...
-
bioRxiv - Physiology 2020Quote: ... Secretion of glycerol and non-esterified fatty acid from cultured adipocytes into HBSS was determined with assay kits from Sigma-Aldrich (FG0100) and FUJIFILM Wako Diagnostics-NEFA Reagent (NEFA-HR(2)) ...
-
bioRxiv - Physiology 2021Quote: The G3P concentration was quantified using the Glycerol-3-Phosphate Assay Kit (MAK207) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of G3P was expressed in ng/μg protein.
-
bioRxiv - Physiology 2021Quote: ... A 30-μL portion of supernatant was used to measure circulating glucose levels with glucose oxidase assay kit (Sigma-Aldrich, GAGO-20) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ATP levels were measured in control and constricted breast cancer cells (3 x 105 cells) using the ATP fluorometric assay kit (Sigma-Aldrich, MAK190), following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
-
bioRxiv - Immunology 2021Quote: The levels of cytokine and chemokines in BAL samples from NV1-treated and control SARS-CoV-2-infected mice were analyzed by Luminex multiplex assays using 36-plex MILLIPLEX® MAP Mouse Cytokine / Chemokine Magnetic Bead kit (EMD Millipore). BAL samples were quickly vortexed for 10s ...
-
bioRxiv - Microbiology 2023Quote: ... ATP in the supernatant was determined with an ATP bioluminescent assay kit according to instructions from the manufacturer (Sigma-Aldrich, FLAA-1KT).
-
bioRxiv - Cell Biology 2023Quote: ... Liver function was assessed by measuring the concentration of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum by using an ALT Activity Assay Kit (Sigma- Aldrich, MAK052) and an AST Activity Assay Kit (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: Chromatin immunoprecipitation assay was carried out to assess the functional status of p53 using Magna ChIP™ G Tissue Kit (Millipore, USA). Briefly ...
-
bioRxiv - Immunology 2023Quote: ... The first kit used was the Milliplex® SARS-CoV-2 antigen panel 1 IgA assay (Millipore Sigma, Cat No. HC19SERA1-85K) with the Wuhan-1 strain RBD and N proteins included ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Determination of SL drug cytotoxicity in 2D samples was performed using the cell counting kit-8 (CCK8) viability assay (Sigma Aldrich, #96992). CCK8 uses water-soluble tetrazolium to quantify the number of live cells by producing formazan dye generated by dehydrogenases in cells ...
-
bioRxiv - Biochemistry 2024Quote: The concentration of both activated proteins—PA-BFP and SumireF—was determined by the bicinchoninic acid assay using the QuantiPro™ Kit (Sigma–Aldrich), following the supplier’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... pyruvate and isocitrate concentration was determined by colorimetric assay kits that were used according to the respective manufacturer’s protocol (Sigma; MAK086, MAK071, MAK061). 1 g of soil was mixed with 1 ml of VE H2O ...
-
bioRxiv - Plant Biology 2024Quote: ... the total proteins in the samples were measured through the BiCinchoninic acid Assay using a kit (Sigma-Aldrich, Saint Louis, MO, USA). The assay was conducted according to the method provided by the manufacturer ...
-
bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assays were performed in liver cryosections from frozen tissue using Duolink® In situ Red Starter Kit Mouse/Rabbit from Sigma-Aldrich, according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2023Quote: Caspase-9 activity was measured in liver lysate according to manufacturer’s protocol (Merck Caspase-9 Colorimetric Activity Assay Kit APT139, EMD Millipore Corporation, USA). In brief ...
-
bioRxiv - Physiology 2023Quote: Dissected fat bodies pooled from 10 3rd instar larvae were homogenized for glycogen and glucose measurements according to the Glucose assay kit (Sigma-Aldrich #GAGO20).
-
bioRxiv - Cancer Biology 2023Quote: ALT and AST activity and plasma AFP levels were determined in plasma with the commercially assay kits (Sigma-Aldrich, MAK052; and MAK055) following manufacturer’s recommendations.
-
bioRxiv - Cell Biology 2023Quote: ... 50 mg of cryogenically pulverized lung tissue was homogenized in 500 μL ultrapure H2O and hydroxy proline content was measured using a Hydroxyproline Assay Kit (Sigma-Aldrich, MAK357) following manufacturer’s instructions.
-
bioRxiv - Evolutionary Biology 2023Quote: ... Final concentration was measured by Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific. RNA integrity was assessed by gel electrophoresis with 1:1 formamide (Merck Millipore) and 1 µl of loading dye on 1.5% agarose gel ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
-
bioRxiv - Genomics 2022Quote: ... Cells were then stained with propidium iodide and Hoechst 33342 from the Live-Dead Cell Viability Assay Kit (Millipore Sigma, Catalog CBA415) according to manufacturers’ instructions and immediately imaged at 10x magnification with a 0.17 numerical aperture CFI60 objective on a Keyence BZ-X710 microscope ...
-
bioRxiv - Cancer Biology 2023Quote: ... T cell migration potential across an endothelial barrier in vitro was assessed using a QCM™ Leukocyte Trans-endothelial Migration Colorimetric Assay kit (Cat: ECM557, Millipore Sigma) following the manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2022Quote: ... The sonicated nuclear fractions were centrifuged at 10k xg at 4 ⁰C for 10 min and then chromatin immunoprecipitation (ChIP) assay was followed with manufacturers’ guidance for EZ-Magna Chip A/G kit (Millipore, #17-10086) with minor modification ...
-
bioRxiv - Immunology 2023Quote: ... The supernatant was collected and serum insulin levels were determined in triplicate using a rat/mouse insulin Enzyme-Linked Sorbent Assay (ELISA) kit from Millipore (EZRMI-13K) according to the instructions of the manufacturer ...
-
bioRxiv - Molecular Biology 2024Quote: The determination of the diauxic shift was performed by measuring glucose levels in the culture media using the Glucose (GO) Assay Kit (Sigma-Aldrich; GAGO20). The start of the PD phase was defined as the time when cells began regrowing and oxygen consumption increased ...
-
bioRxiv - Microbiology 2024Quote: The intracellular ATP level was determined by the luminescence method using the Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit (Sigma Aldrich, USA). 5 ml of cell suspension grown to OD600≈0.5 was washed twice with saline and suspended in 2 ml of saline ...
-
bioRxiv - Cell Biology 2024Quote: Apoptosis assays were performed in duplicate by detection of caspase-3 activity using a fluorometric [µM AMC] (or/colorimetric [µM PNA/min/ml] unless stated) assay kit (Cat#APPA015-1KT/CASP3C-1KT, Sigma, London, England) according to the manufacturers’ protocols ...
-
bioRxiv - Biophysics 2024Quote: The Citrate Synthase Activity in the Total and Supernatant fractions was evaluated using the Citrate Synthase Activity Assay Kit (Sigma-Aldrich MAK193). This assay detects -SH groups produced during the enzymatic conversion of oxaloacetate and acetyl-CoA to citrate by citrate synthase ...
-
bioRxiv - Biochemistry 2024Quote: ... Cell pellet was then resuspended in assay buffer from the pyruvate dehydrogenase activity kit (Cat. No. MAK183, Sigma-Aldrich, Oakville, ON, CA). Cell suspension was sonicated at 45% amplitude with 3 pulses on for 10 secs and off for 10 secs ...
-
bioRxiv - Cancer Biology 2024Quote: The autophagic activity in cell cultures and its response to different drug treatments was assessed using an autophagy assay kit (Sigma, Missouri, USA) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... non-sEV fraction and the whole conditioned medium from hBM-MSCs and NHDFs were quantified using QuantiProTM BCA Assay Kit (Cat.: QPBCA-1KT, Lot.: SLCH0421, Sigma-Aldrich, USA).
-
bioRxiv - Cell Biology 2023Quote: ... The cell proliferation assay was done using the standard MTT assay (Sigma-Aldrich/Merck, Catalog No. 11465007001). MTT assay was performed on each well following the manufacturer’s protocol.
-
bioRxiv - Biochemistry 2024Quote: The proximity ligation was assay was carried out using the Sigma duolink proximity ligation assay (Sigma, DUO92101) according to the manufacturer‘s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... MultiScreen® HTS 96 multiwell plates (pore size 0.45 μm) with high protein-binding membrane (hydrophobic Immobilon-P PVDF membrane) were purchased from Millipore (Amsterdam, The Netherlands), conical 96-well Nunc plates from Thermo ...