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Citations for Millipore Sigma :
2051 - 2100 of 2423 citations for OAT Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the bound proteins were eluted with 400 μL of wash buffer supplemented with 250 μg/mL HA peptide (Sigma-Aldrich) by incubation at room temperature for 10 min ...
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bioRxiv - Synthetic Biology 2022Quote: Translated peptides were purified from in vitro translation reactions by enrichment using Anti-FLAG® M2 Magnetic Beads (Millipore Sigma) according to the manufacturer’s protocol with slight modifications ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were washed three times with 0.5 ml of FLAG wash buffer and the bound protein was eluted with 1X SDS-PAGE loading buffer supplemented with 100 µg·ml-1 3X FLAG peptide (Sigma-Aldrich) on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... Dynabeads following the first pulldown were resuspended in Re-ChIP elution buffer (20 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 100 µg of 3X FLAG peptide [Sigma, #F4799]), and incubated in 4 °C for 1 hour with rotation to selectively elute FLAG-fusion protein ...
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bioRxiv - Molecular Biology 2022Quote: ... FLAG-tagged proteins were eluted by 15 minutes shaking (500 RPM) in 25 μl of B100 + HALT containing 1 mg/ml 3xFLAG peptide (Sigma) at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... glutathione or flag peptide were removed by buffer exchange and the purified proteins were concentrated on Amicon Ultra 50K filters (Millipore). Purified proteins were recovered in 20 mM Hepes ...
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bioRxiv - Cell Biology 2022Quote: ... a2 and a3 spliced-variants of the Pde5 gene fused at their 5’ end to the short DNA sequence coding for the 3XFLAG peptide (Sigma) [20] ...
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bioRxiv - Neuroscience 2020Quote: Amyloid-beta protein fragment 1-42 (Aβ42 peptide) was purchased as a lyophilized solid and more than 95% purity was guaranteed by HPLC from Sigma-Aldrich (High Performance Liquid Chromatography) ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]38, 4 µg/mL; concanavalin A [ConA], 2 µg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]33, 4 μg/mL; Concanavalin A [ConA], 2 μg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Genetics 2021Quote: ... NMDA receptor subunit 2 C-terminal peptide “SIESDV” was synthesised (Pepceuticals) and fully dissolved in 90% v/v methanol + 1M HEPES pH7 (both Sigma). Dissolved peptide was coupled to Affi-Gel 10 resin (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Neuroscience 2020Quote: ... The phosphorylated peptides were released from IMAC beads by 3 × 100 μL 500 mM dibasic sodium phosphate (Na2HPO4, Sigma, S9763), pH 7.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed with TBS-lysis buffer and proteins were eluted from beads by addition of 3×Flag peptides (150 μg/mL, Sigma) for 1 h at 4°C and then subjected to tryptic digestion followed by LC-MS/MS ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then eluted off the anti-Flag beads using competitive 500 µg/mL 3xFlag peptide (Sigma-Aldrich, F4799-4mg) elution in 150 µL lysis buffer by rotation for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins in the insoluble cell wall fraction were digested by trypsin and peptides and glycopeptides were desalted using C18 ZipTips (Millipore).
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bioRxiv - Developmental Biology 2022Quote: ... Peptides were recovered by centrifugation followed by two additional washes with 50mM ammonium bicarbonate and 0.5M NaCl (Sigma-Aldrich, Switzerland). The two filtrates were combined ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were eluted using 500 μl of IP Buffer containing 0.25 mg/ml of the 3×Flag peptide (Sigma-Aldrich) and 1× cOmplete EDTA-free for 15 min at 4°C ...
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bioRxiv - Pathology 2020Quote: ... 150 mM NaCl containing the corresponding detergents and bound proteins were eluted three times with 10 μL 0.5 mg/mL c-Myc peptide (Sigma M2435) in the corresponding detergent containing buffer ...
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bioRxiv - Synthetic Biology 2019Quote: ... and then the elution buffer with the targeted modified peptide was replaced with PB buffer using an Amicon Ultra centrifugal filter (Millipore). Trypsin (2.5% ...
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bioRxiv - Biochemistry 2020Quote: Rabbit polyclonal anti-TcHTE antibodies were obtained using the p1TcHTE peptide (amino acid 120 to 171) expressed as a TRX.HIS-fusion protein in the vector pET-32a vector (Novagen®), following same strategy we described previously (23) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Systems Biology 2019Quote: ... These were then spiked with synthetic heavy labelled analogues of the peptide targets and a retention time standard (MSRT1, Sigma) for scheduled selected reaction monitoring ...
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bioRxiv - Cancer Biology 2020Quote: ... The equivalent of 5 mg of protein was further enriched for phosphorylated tyrosine peptides by overnight immunoprecipitation (pY-IP) using a cocktail of anti-phosphotyrosine antibodies (4G10, Millipore; PT-66 ...
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Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: ... The bound peptides were eluted 3 times from the beads with 0.1% trifluoroacetic acid (TFA; Sigma-Aldrich, Saint Louis, USA). The eluted fractions were pooled together and vacuum-dried ...
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bioRxiv - Cell Biology 2021Quote: ... the bound proteins were eluted by incubation with 250 μl of 3 x FLAG peptide solution (200 μg/mL) (Sigma) for 15-20 min twice ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH, pH7.2, 150 mM NaCl, 500 μg/mL 3×FLAG peptide [Sigma-Aldrich]). The final supernatant containing 3×FLAG-TBC1D18 was transferred to a fresh tube.
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing target proteins were combined and concentrated using an Amicon Ultra-15 Centrifugal Filter Unit (10 kDa for precursor peptide, 30kDa for modification enzymes, MWCO, Millipore). The resulting protein sample was stored at −70 °C.
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bioRxiv - Biophysics 2021Quote: ... Between 0.25 to 0.5 mL of a 100-200 ng/mL solution of FLAG peptide (Sigma-Aldrich, St. Louis, MO) in KBT was added to the rinsed beads ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EDTA and 2 mM PMSF) and eluted with 1 mg/ml FLAG® Peptide (Sigma, cat. no. F3290) in wash buffer overnight on a rotator at 4°C for anaylsis by mass spectrometry.
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bioRxiv - Biophysics 2021Quote: The peptide GAL was purchased from USV Limited (Mumbai, India) by custom synthesis and other chemicals were obtained from Sigma Chemicals or other sources with the highest purity available.
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bioRxiv - Immunology 2020Quote: ... overlapping 15mer peptides offset by 11 residues were designed and synthesised (Mimotopes) and reconstituted in sterile 100% DMSO (Sigma-Aldrich) at a concentration of 100 mg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were manually dissociated through a 70µM cell strainer and OTI T-cells were activated and expanded with 20ng/mL of SIINFEKL peptide (Sigma-Aldrich) and 100 IU/mL recombinant human IL-2 (Biolegend ...
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bioRxiv - Immunology 2020Quote: Each peptide sample was desalted on Empore SPE C18 cartridges (standard density, bed inner diameter 7 mm, volume 3 ml; Sigma), then concentrated by vacuum centrifugation and reconstituted in 40 µl 0.1% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... STM14_0336 was amplified by PCR and cloned into pBRA vector under the control of PBAD promoter (M. Marroquin, unpublished) with or without PelB signal peptide sequence from pET22b (Novagen) (Bayer-Santos et al. ...
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bioRxiv - Immunology 2021Quote: ... HLA typed HLA-A*02:01+ PBMCs from COVID-19 recovered individuals were incubated with SARS-CoV-2 peptide pools (2µM / peptide) and cultured for 10-14 days in RPMI-1640 supplemented with 1x Non-essential amino acids (NEAA; Sigma), 5 mM HEPES (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated ex vivo for 4h at 37°C with 0.5 μg/ml of SIINFEKL peptide and 10 μg/ml of Brefeldin A (Sigma-Aldrich) in complete RPMI ...
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bioRxiv - Immunology 2021Quote: ... mice were intraperitoneally injected with 100 µg Ag85b280-294 (FQDAYNAAGGHNAVF) or 2W1S (EAWGALANWAVDSA) peptide in 100 µl of a 1:1 emulsion with Complete Freund’s adjuvant (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... WT and Bhlhe40−/− mixed BM chimeras were generated as described above and intraperitoneally injected with 100 µg Ag85b280-294 peptide in 100 µl of a 1:1 emulsion with Complete Freund’s adjuvant (Sigma-Aldrich). On day 11 post immunization ...
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bioRxiv - Immunology 2021Quote: Splenocytes from mice immunized with Lm-Ova 6 wks prior were co-incubated with SIINFEKL peptide (108M) with Golgi Plug/Stop and i) with or without cycloheximide (Translation inhibitor, 10µg/mL, Sigma-Aldrich) or Actinomycin D (Transcription inhibitor ...
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bioRxiv - Genetics 2019Quote: ... The beads were washed 3x with wash buffer and proteins were eluted with FLAG peptide (200 mg/ml, Sigma-Aldrich) in TBS ...
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bioRxiv - Immunology 2022Quote: ... 1 µg/mL SIINFEKL peptide in 200 µL IMDM media with 10% heat-inactivated FBS and 0.05 mM betamercaptoethanol (Sigma-Aldrich) were added to each sample and incubated for a total of 6 h at 37 °C with 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... rPAI-1 was further purified by binding to anti-FLAG agarose beads and elution with 3X FLAG peptide (Millipore Sigma). rPAI-1 was quantified spectrophotometrically (ε280nm = 43,000 M−1 cm−1) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.05% [w/v] digitonin [Sigma: D141], 10% [w/v] glycerol, and 0.4 mg/mL, and 1x FLAG-peptide [Sigma: F3290]) (30 min ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were acidified to 0.67% trifluoroacetic acid (TFA) and then desalted using C18ZipTip (Millipore cat no. ZTC18 5096). Dried peptides were frozen ...
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bioRxiv - Neuroscience 2022Quote: ... LMAN and RA were visualized by immunostaining for calcitonin gene related peptide (Sigma, RRID: AB_259091, 1:5000 to 1:10000) (Bottjer et al. ...
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bioRxiv - Microbiology 2022Quote: ... Samples were eluted in 45 µl of Elution Buffer (100 µg/mL FLAG peptide in Final Wash Buffer; Millipore Sigma) for 25 minutes at room temperature with continuous gentle agitation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (f-MLP) (F3506) and prostaglandin E1 (PGE1) were all purchased from Sigma Aldrich. The purinergic receptor agonist ...
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bioRxiv - Neuroscience 2022Quote: ... peptides were acidified with 2 μl of 1% TFA then desalted using C18 ZipTips® (Millipore, cat no. Z720070-96EA).
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bioRxiv - Molecular Biology 2022Quote: ... yejASm lacking the fragment encoding the first 30 amino acids (signal peptide) was PCR-amplified from Sm1021 genomic DNA and cloned into a pET29b(+) vector (Novagen) generating a C-terminal 6His-tag ...