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Citations for Millipore Sigma :
2051 - 2100 of 9209 citations for Human Cathepsin B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... at which point cells were fixed and stained for SA-B-Galactosidase (Millipore Sigma, CS0030). Plates were incubated at 37°C without CO2 for 48h ...
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bioRxiv - Physiology 2024Quote: ... The internal solution was supplemented with 250 µg/ml amphotericin B (Sigma, St. Louis, MO). STOCs were measured in the voltage-clamp mode and were analyzed with the threshold detection algorithm in Clampfit 10 (Axon Instruments ...
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bioRxiv - Plant Biology 2024Quote: ... Stable transformants were selected using 15 mgmL-1 Hygromycin B (Sigma-Aldrich cat. no. H9773).
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bioRxiv - Pathology 2024Quote: ... p-RAPTOR (#, 1:1000), RAPTOR (#, 1:1000) or b-actin (#A5316, Sigma-Aldrich, 1:5000). Blots were revealed on a Chemidoc imager (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... B) Liquid cultures were extracted by first capturing metabolites using XAD-7HP resin (Sigma-Aldrich), and then extracting the resin with methanol ...
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bioRxiv - Immunology 2024Quote: ... Naïve B cells were activated by addition of 5-25 μg/ml LPS (Sigma-Aldrich) and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... washed with PBS and lysed in 5 mL Hypotonic lysis buffer B with DPEC (Sigma) for 10 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Stable cells were generated by selection with 800µg/mL of Hygromycin B (Sigma-Aldrich, H7772). The cells were again transfected with the SBE-RFP reporter with the piggyback transposase and selected for stable integration of both reporters by three rounds of fluorescence-assisted cell sorting for YFP and RFP double positive cells (dual reporter cells).
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bioRxiv - Pathology 2023Quote: ... After 30 min of washing with Duolink In Situ Wash Buffer B (DUO82048, Sigma-Aldrich), sections were mounted using Duolink In Situ Mounting Medium with DAPI (DUO82040 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested by trypsinization and washed with ice-cold PBS-B (DPBS (Sigma-Aldrich) containing 1% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell culture medium was supplemented with 2 μg/ml of cytochalasin B (Sigma-Aldrich, #C6762) 16 hrs prior to fixation to block cells at cytokinesis stage of cell division ...
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bioRxiv - Neuroscience 2023Quote: ... A short column of the K+ sensor (Potassium ionophore I, cocktail B; Sigma-Aldrich, #99373) was taken into the tip of the salinized pipette by using slight suction ...
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bioRxiv - Microbiology 2023Quote: ... Briefly, double dilutions of colistin (Duchefa Biochemie, Haarlem, The Netherlands) and polymyxin B (Sigma-Aldrich) were performed in LB media horizontally in a 96-well plate over a range of concentrations 16-0.015 µg/ml in a final volume 100 µl ...
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bioRxiv - Cancer Biology 2023Quote: ... The gelatin coating solution was prepared by mixing gelatin solution type B (G1393, Sigma Aldrich) with gelatin from pig skin ...
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bioRxiv - Molecular Biology 2023Quote: ... For transformant selection, hygromycin B (H005, MDbio, China) and geneticin (Sigma-Aldrich, St. Louis, MO) were added to the final concentration of 300 μg/ml and 200 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.0) containing 1% n-Dodecyl-B-D-Maltoside (DDM; Sigma and 1% digitonin (Sigma), incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.0) containing 1% n-Dodecyl-B-D-Maltoside (DDM; Sigma and 1% digitonin (Sigma), incubated on ice for 30 min ...
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bioRxiv - Immunology 2024Quote: ... supplemented with penicillin-streptomycin and glutamine on gelatin coated plates (Millipore Sigma, ES-006-B). For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of TNFRII/TNFRSF1B-Fc chimera protein (726-R2-050 ...
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bioRxiv - Immunology 2024Quote: ... a mix of LPS and 20 ug/mL of cholera toxin B (CTB) from Sigma was made in Opti-MEM™ before vortexing and then incubating for 5 min at room temperature to allow the formation of the complex (CTB/LPS).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... which was then replaced with Rhodamine B-spiked sugar (1mg/25ml of sugar, Sigma R6626) for the next 24h ...
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bioRxiv - Cell Biology 2024Quote: ... followed by washing twice for 10 min in 1x wash buffer B (Sigma Aldrich, #DUO82048) and then once for 1 min in 100 x diluted wash buffer B ...
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bioRxiv - Neuroscience 2024Quote: ... and CNPase (myeloarchitecture marker; 1:500, clone 11-5 B, Millipore, cat#MAB326, Darmstadt, Germany), for 18 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was manually homogenized using a two-step Dounce homogenizer (A and B) (Sigma #D9063) in NIMT buffer (in mM ...
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bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no. HCVD3-67CKHCYTOMAG-60K, Millipore Corp, St. Charles, MO). According to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Cytokines in BAL fluid samples were measured (pg/ml) in singlicate by Luminex using a Millipore human cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA) according to manufactures instructions ...
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bioRxiv - Pathology 2019Quote: ... and TGF-β1 in the samples was measured using magnetic bead-based multiple immunoassays (MILLIPLEX® MAP kit, Human High Sensitivity T Cell Magnetic Bead Panel: EMD Millipore Corporation, Germany). Each assay included two calibration curves for each of the proteins to be measured (calibration ranges ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
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bioRxiv - Microbiology 2019Quote: ... ELISAs of cells harvested from tryptone plates were treated with 100 Kunitz units/mL DNaseI (D5025, Sigma Aldrich) for 1 hr statically at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IL-1β and TNF-α using indirect sandwich enzyme-linked immunosorbent assay (ELISA) (Sigma-Aldrich; #RAB0308 and #RAB0477) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Cell Biology 2019Quote: ... to each sample in the absence and presence of 20 µM cytochalasin B (Sigma Catalog #: C6762), a potent inhibitor of glucose transport ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% Tween) for at least 5 min and then with NBT/BCIP (Sigma, N6639, B-8503) diluted in the NTMT buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the plastic tissue culture-treated plates were coated with 0.1% gelatin (EMD Millipore ES-006-B) at room temperature for at least 30min ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were routinely cultured on dishes coated with 0.1% gelatin type B (Sigma, Cat#G9391-100G), in GMEM (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... All N/Tert-1 cells were also supplemented with 4 μg/ml hygromycin B (Millipore Sigma). All cells not directly purchased from providers were cell type confirmed by Johns Hopkins or MD Anderson cell line authentication services ...
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bioRxiv - Biochemistry 2020Quote: ... cells were treated with 10 ng/ml of the CRM1 inhibitor Leptomycin B (LMB, Sigma Aldrich) in DMSO for 15 min before cell fixation ...
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bioRxiv - Neuroscience 2021Quote: ... mouse α-tubulin clone B-5-1-2 (1:1000; Sigma Aldrich, San Luis, MO, U.S.). For immunofluorescence primary antibodies were labeled with Alexa-conjugated secondary antibodies Alexa 488 ...
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bioRxiv - Neuroscience 2021Quote: ... we injected 2 µl of 1% w/v FITC-conjugated cholera toxin B subunit (Sigma, C1655) in rats subjected to MCAO on day 14 post-stroke and analyzed the distribution of the labeling by confocal microscopy at 21 d post-stroke ...
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bioRxiv - Cell Biology 2021Quote: Mouse splenic B-cells were pretreated or not with 12 μM bromoenol lactone (BEL, Sigma-Aldrich) in DMEM-BSA for 30 min at 37°C before and during assays ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were washed under running water and cells were stained with 1mL sulforhodamine B (Sigma 230162) and incubated at room temperature for 30 minutes ...