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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for ENPP 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 2 mM Glutamine (Sigma), 1% Pen/Step (Sigma) ...
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bioRxiv - Genetics 2023Quote: ... 2 mM Trolox (Sigma)) was added to sample.
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bioRxiv - Molecular Biology 2023Quote: ... 2-mercaptoethanol (SIGMA, M6250), Penicillin-Streptomycin) ...
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bioRxiv - Bioengineering 2023Quote: ... and 2% FBS (Sigma). Chips were ready to use after seven days of culture.
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bioRxiv - Cell Biology 2023Quote: ... 2 mM HEPES (Sigma), 14.5 mM NaOH (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM glutamine (Sigma), and 10 μg/mL gentamicin (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 2 μM Forskolin (Sigma) and 10 ng/ml rhNRG1 (R&D) ...
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bioRxiv - Cell Biology 2024Quote: ● 2-Mercaptoethanol (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM glutamine (Sigma), 100 µg/ml penicillin and 100 U/ml streptomycin (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... 2 mM ATP (Sigma), and 6 μM calmodulin ...
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bioRxiv - Bioengineering 2024Quote: ... + 1% 2-mercaptoethanol (Sigma) were utilized ...
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bioRxiv - Cell Biology 2024Quote: ... 2% PenStrepNeo (Sigma, P4083), 0.02 mg/mL insulin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... 2% red alizarin (SIGMA) or oil red (Lipid oil red staining kit ...
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bioRxiv - Genomics 2024Quote: ... containing 2% BSA (Sigma), 2mM Glutamax (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... Rosetta 2 DE3 (Novagen). Bacteria were lysed in phosphate buffered high saline (PBHS ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa strains were routinely refreshed from frozen stocks at -80°C and maintained on YPD (1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, 2% Bacto agar) plates or Pseudomonas isolation agar (Sigma-Aldrich) for in vitro experiments and LB agar (10 g/liter Bacto tryptone ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of cell culture medium were incubated at 37°C for 3 hours with 20 µL reaction mix containing sodium citrate (10 mM; pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Immunology 2024Quote: Gonadal white adipose tissue was digested into pre-warmed 2 mg/ml BSA 2% FBS RPMI supplemented with 2 mg/ml collagenase type II (Sigma, C6885) and placed under shaking at 180 rpm for 40 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Immunology 2019Quote: ... 1 μM oligomycin and 100 mM 2-deoxy-D-glucose (2-DG; Sigma).
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bioRxiv - Cancer Biology 2020Quote: ... SASI_Hs01_00061211, #2; SASI_Hs01_00061214), and PDX1(#1; SASI_Hs01_00108076, #2; SASI_Hs01_00108080) were purchased from Sigma. shRNA-containing plasmids targeting human and mouse RNF125 (Human ...
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bioRxiv - Developmental Biology 2022Quote: ... 2% SDS) supplemented with protease inhibitor and phosphatase inhibitors 2 and 3 (Sigma). Samples were subjected to dounce homogenization ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 % Span80 (Fluka) surfactant and 4 % 2-Hydroxy-2-methylpropiophenone (97%, Sigma-Aldrich) photoinitiator ...
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bioRxiv - Neuroscience 2020Quote: ... BCH (Slc7a5 inhibitor 2-amino-bicyclo[2,2,1]heptane-2-carboxylic acid, Sigma-Aldrich) was stored as a 100 mM stock solution in 1N NaOH ...
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bioRxiv - Physiology 2022Quote: ... GSH in deproteinated samples was derivatized with 2-vinylpyridine (Sigma-Aldrich, #13229-2) prior to assessment ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1% (v/v) photo-initiator (2-hydroxy-2-methylpropiophenone, Sigma, Product No.: 405655) in buffer (75 mM Trizma HCl pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: 2 µM mTLR9-ECD was mixed with 2 µM ssDNA oligo (Sigma-Aldrich) in 10 mM MES pH 6 ...
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bioRxiv - Physiology 2020Quote: ... elegans day 2 adult worms were fixed with 2% paraformaldehyde (Sigma Aldrich, P6148) and 1% glutaraldehyde (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 500 µL of 2% 3-aminopropyltriethoxysilane (Sigma; 919-30-2) in 95% ethanol for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml/well overlay [MEM containing 2% FBS and 0.4% Tragacanth (Sigma, Israel)] was added to each well and plates were further incubated at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 2 ml/well overlay [MEM containing 2% FBS and 0.4% Tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) (Sigma, SML0601); an irreversible O-Carnitine palmitoyltransferase-1 (CPT1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The ATP regeneration system (2 mM ATP, 2 mM phosphoenolpyruvate [PEP, Sigma-Aldrich, #P7127] ...
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bioRxiv - Bioengineering 2022Quote: ... blocked with 2% Abdil (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) for 1 hour ...
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bioRxiv - Systems Biology 2022Quote: ... Fenofibrate (2-[4-(4-Chlorobenzoyl)phenoxy]-2-methylpropanoic acid isopropyl ester) (Sigma # F6020) was injected peritoneally (4% DMSO/PBS ...
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bioRxiv - Microbiology 2022Quote: ... 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, Sigma-Aldrich), 1.2 mg/ml Sodium Bicarbonate (Sigma-Aldrich/MilliporeSigma ...
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bioRxiv - Cell Biology 2023Quote: A 100 mM stock of 2-bromopalmitic acid (2-BP, Sigma-Aldrich 248422) or methyl-palmitate (MP ...