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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for Cow Calcipressin 1 RCAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 1:800 (Sigma, SAB4200181); anti-GFP ...
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bioRxiv - Physiology 2024Quote: ... laminin (1:200, Sigma), CD45 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... + 1% beta-mercaptoethanol (Sigma) via gentleMACS Octo Dissociator in M-Tubes (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... 1% protease (Sigma-Aldrich) was applied to those segments to remove overlaying glia ...
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bioRxiv - Cell Biology 2023Quote: ... Ferrostatin-1 (Sigma, SML0583), idebenone (Tokyo Chemical Industry ...
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bioRxiv - Genetics 2021Quote: ... Mix-n-Stain CF 555 Antibody Labeling Kit and Mix-n-Stain CF 647 Antibody Labeling Kit (Sigma-Aldrich, MX555S100 and MX647S100) were used according to the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... an Alexa Fluor™ 568 antibody labeling kit and a Mix-n-Stain™ CF®633 Dye antibody labeling kit (Sigma) were used according to manufacturer’s protocols.
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bioRxiv - Neuroscience 2020Quote: Primary cultures fixed and permeabilised as described for IF were used to perform PLA using a commercial kit (Duolink® PLA starter kit, Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA of control and insertion lines was extracted using a commercial kit (Spectrum™ Plant Total RNA Kit, Sigma life science) following the supplier’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines were routinely tested negative for mycoplasma contamination using Universal Mycoplasma detection kit (ATCC, 30-1012K) or LookOut Mycoplasma Detection Kit (Sigma-Aldrich, MP0035). The INF2-KO U2OS cell line made by CRISPR-Cas9 is described elsewhere (Chakrabarti et al. ...
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bioRxiv - Developmental Biology 2022Quote: An ATP calibration curve was performed using the stabilized ATP standard stocks provided in the kit ATP Bioluminescence Assay Kit HS II (Millipore Sigma #11699709001) and following the supplied protocol ...
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bioRxiv - Molecular Biology 2023Quote: AKT:FBP1:ALDOB:PP2A-C interactions in liver tissue were detected with an in situ PLA kit (Duolink® In Situ Red Starter Kit Mouse/Rabbit, Sigma, DUO92101) (Su et al. ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: Activity of ALPL and calcium ions concentration in cell lysates on day 7 of osteogenic differentiation were determined using reagent kits “Alkaline Phosphatase-New Liquid Form” (Vector-Best, Russia) and Calcium Colorimetric Assay Kit (Sigma-Aldrich, USA), respectively ...
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bioRxiv - Physiology 2023Quote: ... FGF21 was measured by using a commercially available enzyme-linked immunosorbent assay kit (rat/mouse FGF21 enzyme-linked immunosorbent assay kit; Millipore, Billerica, MA), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: RNA was extracted using Qiagen RNeasy Mini Kit (Maryland, USA) according to the manufacturer’s protocol and DNase-treated using the DNase I kit (Millipore Sigma, Massachusetts, USA). The quantity and quality of RNA were determined using the Nanodrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6], and mouse anti-2A [1:1000, Sigma, #3H4]), washed with TBST ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with Alexa 488/647-conjugated goat anti-mouse or anti-rabbit (1:100-1:400, Thermo Fischer) and Alexa 488/546-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by immunocytochemistry with mouse monoclonal anti-vinculin antibody (1:200 dilution in 1% BSA–PBS) (Sigma; V9264, clone hVIN-1) and Alexa 555–labelled goat anti–mouse IgG (#A21422 ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit polyclonal anti-FLAG (F7425, 1:200), mouse monoclonal anti-vinculin (V4505, 1:200), and mouse monoclonal α-SMA (A5228, 1:200) from Sigma; rabbit polyclonal anti–SOX9 (#82630 ...
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bioRxiv - Neuroscience 2021Quote: ... cross- and longitudinal sections of the sciatic nerve were stained with Tuj-1 (1:500, sigma, T5076) and ChAT (1:50, Millipore, AB144P). The numbers of Tuj-1-positive axons ...
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bioRxiv - Neuroscience 2021Quote: ... chicken anti-GFAP (Chemicon, AB5541, 1:3000, RRID:AB_177521), mouse anti-SMI-32 (Covance, SMI-32R, 1:2000) and Hoechst 33342 (Sigma Aldrich, 1:300) for nuclear labeling ...
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bioRxiv - Molecular Biology 2020Quote: ... empty biotin modified hydrogel spheres were modified with 1:1 volume ratio of 1 mg/ml streptavidin (Sigma-Aldrich, cat #85878) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... anti-GFP (in house made rabbit polyclonal antibody; 1:1,000-4,000) anti-Vinculin (mouse monoclonal; clone hVIN-1; Sigma-Aldrich; 1:10000), anti PMF1/NNF1 (in house made mouse affinity purified monoclonal ...
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bioRxiv - Molecular Biology 2019Quote: ... was obtained from the Swansea University culture collection and grown on Sabourand dextrose agar (SDA, 40 gL−1 D-glucose, 10 gL−1 mycological peptone, 5 gL−1 technical agar (Sigma,UK), 0.5 gL−1 chloramphenicol ...
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bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of the samples (1:2 to 1:64) and a standard row containing 1 to 40 nmol Neu5Ac (Sigma Aldrich) were prepared in extraction buffer ...
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... A small aliquot from the final suspension was taken and diluted 1:4 in CCM and then 1:1 in Trypan Blue (T8154, Sigma-Aldrich). A live/dead cell count was determined by hemocytometer and a cell viability was assessed ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Cell culture supernatant was diluted 1:1 in IP buffer (50 mM Tris-Cl, 75 nM NaCl, 1% Igepal, protease inhibitor mixture (Sigma, 11836153001), pH 7.4 ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636; Map-2 1:250, Sigma Aldrich M4403 ...
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bioRxiv - Physiology 2021Quote: ... anti-TRPV4 (1:200, Alomone, #ACC-034) and anti-mCherry (1:500, NOVUS, #NBP1-96752) or anti-Flag (1:1000, Sigma, #F1804), at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... IF 1:500), mouse anti-cortactin (05-180, IF 1:400), mouse anti-talin (T3287, IF 1:200) all from Sigma-Aldrich; rabbit anti-EEA1 (ALX-210-239 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA, 0.5 mM DTT, 1 mM NaF, 1 mM PMSF and 1% Protease Inhibitor Cocktail from Sigma, pH 7.4) for 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... PC12 cells were incubated in serum-reduced medium (1% horse-serum, 1% FBS) supplemented with 100 ng.ml-1 ß-NGF (Sigma-aldrich) during five days on PDL coated surface.
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bioRxiv - Biochemistry 2022Quote: ... mouse anti-GBP130 (1:500), mouse anti-EXP2 (1:5000, 37), rabbit anti-Nluc (1:400, 65) and mouse anti-HA (1:500, Sigma-Aldrich). After primary antibody was removed ...
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bioRxiv - Biochemistry 2022Quote: Reporter cells were plated in triplicate at 1 × 105 cells ml−1 in media with or without 100 ng ml−1 doxycycline (dox) (Sigma-Aldrich). Every 3 d ...
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bioRxiv - Cell Biology 2022Quote: For vinculin staining hMSCs are fixed with ice cooled mixture of 1:1 (v/v) (4% PFA: permeabilizing buffer (1% Triton-X-100-Sigma Aldrich) for one minute on ice ...
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bioRxiv - Neuroscience 2022Quote: ... antibodies (α-GluA2, Merck clone 6C4, 1:200; α-GluA1, Cell signaling #13185, 1:150, α-myc, Sigma #M5546, 1:1000) were applied to neurons for 10 min at room temperature followed by a washing step in PBS supplemented with 0.1 mM CaCl2 and 1 mM MgCl2 and paraformaldehyde fixation.
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-α-tubulin (T6199; IF: 1/4000) and pAb anti-Flag-tag (F7425; IF: 1/500, WB: 1/1000) were from Sigma-Aldrich, and a pAb anti-GFP (632592 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GSTπ at 1:3000 (MBL, #311), rabbit anti-IBA1 at 1:500 (Wako, #01919741) and rabbit anti-GFAP at 1:1000 (Millipore, #OBT0030). Sections were washed three times with PBS and stained using biotinylated anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were incubated overnight with primary antibodies (Anti-DNALI1: 1: 100, Abcam, Cat No: ab87075; Anti-PACRG: 1: 200, generated by our laboratory; Anti-α-tubulin, 1:200, Sigma, Cat No ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated with the rabbit anti-NeuN (1:800, Beyotime Biotechnology) or monoclonal anti-Dicer 1(1:300, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Strains were seeded in lysogeny broth (LB, 10 g L-1 NaCl, 10 g L-1 tryptone, 5 g L-1 yeast extract, Sigma-Aldrich) at 30°C with shaking overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... anti- Spz (rat, 1:50, requested from Satoshi Goto), Anti-Elav (rabbit, 1:50, DSHB) and Caspase 3 (rabbit, 1:500, Sigma Aldrich). Samples were washed four times for 10 minutes each in PBST and incubated at room temperature in secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected into 293T cells at a 1:1:1:1.6:4.6 ratio using 293 Free transfection reagent (EMD Millipore Cat #72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were co-transfected into 293T cells at a 1:1:1:1.63:4.63 ratio using 293 Free transfection reagent (EMD Millipore Cat# 72181) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were then derivatized for gas chromatography analysis using 50 µL solution of 1:1 pyridine: N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (chemicals obtained from Sigma Aldrich) and incubated at 60 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 1 ml acetone/water (1:1, Carl Roth, Karlsruhe, Germany) and 0.1 % trifluoroacetic acid (Sigma Aldrich, Dreieich, Germany). 170-200 µL DHB matrix solution was applied to the sections using a pneumatic sprayer system with a nitrogen pressure of 0.7-0.75 bar ...
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bioRxiv - Cell Biology 2020Quote: ... then plated onto LB plates containing the appropriate antibiotic (100 μg ml−1 ampicillin, 50 μg ml−1 kanamycin or 25 μg ml−1 chloramphenicol, Sigma Aldrich). Plasmid DNA was extracted from bacteria using GenElute™ Plasmid Miniprep Kit (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-βactin (AC-15, 1:20000) and mouse anti-FLAG (F-3165, 1:7000; IF 1:300) from Sigma-Aldrich; rabbit anti-TOP1 (A302-589A,1:5000) ...