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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for Ceramide Synthase 3 CERS3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 0.5 mM 3-isobutyl-1-methylxanthine (Sigma, I-5879), 0.5 μM rosiglitazone (Wako ...
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bioRxiv - Bioengineering 2024Quote: ... 0.1 mM 3-isobutyl-1-methylxanthine (IBMX, I5879; Sigma), 5 μM rosiglitazone (ROG ...
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bioRxiv - Biophysics 2024Quote: ... and 3-(maleimido)propionic acid N-hydroxysuccinimide ester (Sigma). For co-grafted brushes ...
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bioRxiv - Neuroscience 2024Quote: ... 3% (v/v) Triton X-100 (Millipore Sigma, X100) was added to tissue homogenates and allowed to incubate in ice for 30 minutes with gentle shaking followed by centrifugation at 10,000xg for 15 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 3 mmol MgCl2·6H2O (Sigma, St. Louis, MI M2670), 2.5 mmol EGTA (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was blocked using 3% BSA (Millipore Sigma) in TBS with 0.1% Tween 20 (TBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μM 3-isobutyl-1-methylxanthine (Sigma-Aldrich, #I5879) and 100 μM indomethacin (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... and then incubated in the 3% hydrogen peroxide (Sigma) solution in PBS and washed with PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3 μM CHIR99021 (Sigma Aldrich, Cat. No. 361571). The culture plates were coated with 0.1% gelatin (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... a 5% solution of 3-Aminopropyltriethoxysilane (APTES, Sigma-Aldrich) in tetrahydrofuran (THF ...
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bioRxiv - Microbiology 2024Quote: ... Followed by addition of 0.15% 3-hydroxydiphenol (Sigma-Aldrich), the mixture was analyzed by the absorbance at 520_nm ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5mM 3-isobutyl-1-methylxanthine (IBMX; Sigma-Aldrich, USA), 5µg/ml insulin (SAFC ...
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bioRxiv - Neuroscience 2024Quote: ... For non-neuronal marker (GFAP antibody: Millipore AB5541; Iba1 antibody: GeneTex GTX101495; Sox9 antibody: RnDsystems AF3075; S100β antibody: Sigma S2532), antibodies were diluted at 1/500.
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bioRxiv - Neuroscience 2022Quote: ... and triethyl amine were made in MilliQ ultrapure water (18 megaohm resistance) and 1-octen-3-ol was diluted in paraffin oil (EMD Millipore #PX0045-3). Aliquots of 10 µL of each compound and dilution was pipetted onto a piece of filter paper (5 × 20 mm ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was mounted on charged slides and dried prior to ethanol washes (70%, 80%, 95%, 100% Ethanol, 3 minutes each) and washed in a histological clearing agent (Xylene substitute; Sigma Aldrich; 3 minutes), and then cover slipped with DPX mounting agent (Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The excess fluorescent probe was removed through molecular weight and the buffer was exchanged with PBS (pH 7.4) using 3 kDa centrifugal filter units (UFC500396, Amicon® Ultra – 0.5mL, 3 kDa, Sigma-Aldrich®, Dublin, Ireland). Absorbance was measured at 555 and 280 nm for all tagged samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 SI unit ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Molecular Biology 2022Quote: ... For secondary antibodies anti-mouse antibody (Sigma-Aldrich), anti-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Antibodies were anti-α-tubulin antibody (Sigma-Aldrich, T6199 ...
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bioRxiv - Microbiology 2023Quote: ... or FLAG antibody (Sigma Monoclonal FLAG antibody M2) in TBST with 5% NFDM (w/v) ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 6x-His Tag Polyclonal Antibody or HA Tag Polyclonal Antibody was the primary antibody and the anti-rabbit IgG-gold (10 nm) antibody (Sigma-Aldrich) was the secondary ...
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bioRxiv - Neuroscience 2021Quote: ... This antibody is included in Validated Antibody Database (https://www.labome.com/knockout-validated-antibodies/Cx43-antibody-knockout-validation-Sigma-Aldrich-C6219.html).
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bioRxiv - Cell Biology 2020Quote: ... or cellulose solution in N-hydroxysuccinimide (NHS; Aldrich; 100 mM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma; 100 mM, 90 min, RT)44 ...
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bioRxiv - Microbiology 2019Quote: ... 5α-androstan-17β-ol-3-one (=17β-hydroxyandrostan-3-one) and 3aα-H-4α(3’-propanoate)-7aβ-methylhexahydro-1,5-indanedione (HIP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 5α-androstan-3β,17β-diol (=3β,17β-dihydroxyandrostane ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Immunology 2021Quote: The livers of freshly-sacrificed mice were perfused retrogradely via the IVC(3) with 3 ml of PBS and then 10 ml of 2% paraformaldehyde (Sigma, catalogue# 30525-89-4) in PBS ...
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bioRxiv - Immunology 2020Quote: ... injection of 106 hemocytes from late third instar larvae of the lethal(3)malignant blood neoplasm [l(3)mbn1] mutant larvae in Drosophila Ringer’s solution (Sigma-Aldrich, St. Louis, MI, USA). Booster injections were given 4 ...
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips were then coated with a solution of 3% (v/v) (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich, St. Louis, MO) in ethanol for 20 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... strains containing a degron tag were arrested at 30 °C for 3 hours (total) and then treated with 250 μM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) for 30 minutes before harvesting ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Biophysics 2023Quote: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) dry powders were purchased from Sigma Aldrich (St. Louis, MO, USA). Different amounts of DOPC and DOTAP were used to tune the surface charge of the employed liposomes ...
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bioRxiv - Biophysics 2023Quote: ... the quench buffer also included 3 μL of 0.8% GDN and 3 μL of 300 mg/ml aqueous suspension of ZrO2-coated silica (Sigma-Aldrich, reference no. 55261-U). The resulting mixture was incubated on ice for 1 min to remove lipids and solubilize prestin in GDN before being filtered through a cellulose acetate spin cup (Thermo Pierce ...
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bioRxiv - Plant Biology 2020Quote: ... 150 µM 3′,5′-Dimethoxy-4′-hydroxyacetophenone (acetosyringone; SIGMA, USA) at a 23.7×108 cfu/ml (OD600=0.75 ...
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bioRxiv - Neuroscience 2021Quote: ... for 3 h followed by ATP (2 mM, Sigma Aldrich, A6419-5g ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit polyclonal anti-PCSK1 (PC1/3) (1:1000, Sigma, #SAB1100415), rabbit polyclonal anti-TSSC1/EIPR1 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 200 μM 3-isobutyl-1-methylxanthine (IBMX; Sigma), and their cumulus cells were removed by gentle pipetting ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μM 3-isobutyl-1-methylxanthine (IBMX, Sigma-Aldrich # I5879), 1 μM rosiglitazone (Cayman Chemical # 71742) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were blocked and simultaneously permeabilized with 3% BSA (SIGMA) and 0.2% Triton X-100 (SIGMA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were subsequently blocked and permeabilized with 3% BSA (SIGMA) and 0.2 w/v % Saponin (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... or 3 µg/cm2 poly-L-lysine (Sigma-Aldrich, P6407) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 1x Phosphatase inhibitor cocktail 2 and 3 from Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... and then blocked with 3% (w/v) BSA (Sigma-Aldrich) and 0.05% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-15 µM MG132 (Z-Leu-Leu-Leu-al, Sigma) was added to the media for the indicated hours prior to cell lysis/fixation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 30 μL D2O with 3%TPS (Sigma Aldrich, 450510) and transferred in a standard 5 mm NMR tube (Wilmad-LabGlass ...