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Citations for Millipore Sigma :
2051 - 2100 of 3014 citations for 6 Chloromethylmorphenthridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 5) carnitines: myristoil-L-carnitine (Cayman 26559) 6) lipases: phospholipase A2 (PLA2) from honeybees was purchased from Sigma (P9279). For functional assays ...
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bioRxiv - Immunology 2024Quote: ... cell nuclei were stained with 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, Cat. No. D9542) diluted in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred to 6-well low adhesion plates in ESC media supplemented with 10 µM ROCK Inhibitor (Sigma; Y0503) to initiate differentiation ...
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bioRxiv - Biochemistry 2024Quote: ... homogenised in 6 mL of 2:1 (v/v) chloroform:methanol containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma-Aldrich) as antioxidant ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were seeded on 6-well plates (∼400,000 cells/well) coated with 0.1% gelatin (Sigma-Aldrich cat# G1393), and transfected ∼24 h later using the calcium phosphate method ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Transient transfection with mutant MLH1 constructs (purchased from TWIST Biosciences) and siRNA against CDK4/6 (purchased from Sigma-Aldrich) was conducted using JetPrime PolyPlus transfection reagent ...
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bioRxiv - Cell Biology 2024Quote: ... 561 and 640 nm laser lines were used for excitation of DAPI (4rn,6-Diamidino-2-phenylindole, Sigma-Aldrich), Alexa Fluor 555 and Alexa Fluor 647 respectively ...
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bioRxiv - Immunology 2022Quote: ... cells were lysed in ACK lysis buffer (0.15M NH4Cl [Sigma, A9434-500G], 10mM KHCO3 [Fisher, 298-14-6], 0.1mM Na2EDTA [Sigma, E7889]) for less than 10 minutes and resuspended in T-cell Media (RPMI [Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were labeled by incubation in PBS containing 4′,6-diamidino-2-phenylindole (DAPI) (0.1 μg/ml) (Sigma-Aldrich), and samples were mounted in ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... was lowered to the target area (dorsoventral −2.0 mm from the cortical surface) and 6-OHDA (Sigma-Aldrich, Hungary) was injected by a syringe pump (World Precision Instruments ...
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bioRxiv - Microbiology 2023Quote: ... in absence or in presence of 50 µM pyridoxal-phosphate-6-azopheny-2’,4’-disulfonate (PPADS) (#P178, Sigma-Aldrich), 100 µM oxidized ATP (OxATP ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL of this dilution was then added to 90 μL of buffered D2O (prepared 5 mL with 4.5 mL of D2O and 0.5 mL of 10x protein dilution buffer and 0.2 μg/mL of peptide standard Glu-1-Fibrino peptide (CAS: 103213-49-6, Sigma)) to initiate deuteration at 22 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Each mouse was treated with 500μg 6-OHDA (dissolved in 200 μl sterile PBS containing 0.01% ascorbic acid; Sigma-Aldrich), administered by subcutaneous injection daily for 2 days ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed three time in PBS and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Coverslips were mounted onto glass slides in Mowiol-DABCO ...
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bioRxiv - Cell Biology 2023Quote: ... the slides were washed with TBS and incubated with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... PLL labeled with fluorescein 5-isothiocyanate (PLLFITC) and 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (Rhod) were purchased from Sigma.
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bioRxiv - Microbiology 2023Quote: ... Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich) selection ...
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bioRxiv - Molecular Biology 2023Quote: ... was diluted to 100 ng/μL in 6 mL nuclease-free molecular grade water (Sigma-Aldrich, St. Louis, MO) in a 15 mL tube with 300 g (50 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... β-mercaptoethanol (99%) for 6 h at 4 ℃. The proteins were concentrated by Amicon Ultra (M.W. 3,000 Da) (Sigma-Aldrich) and stored at −80 ℃.
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... the cells were mounted on glass slides using Fluoroshield with 4’,6-diamidino-2-phenylindole (DAPI) (F6057; Sigma-Aldrich). Conventional fluorescent imaging was performed using the Axio Oberver.Z1/7 (Carl Zeiss) ...
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bioRxiv - Cell Biology 2023Quote: ... differentiation was induced using inhibition of the WNT pathway with CHIR99021 (6 μM or 12 μM, #SML1046, Sigma-Aldrich) in RPMI 1640 media supplemented with B27 minus insulin (#A1895602 ...
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bioRxiv - Molecular Biology 2023Quote: EV pellets were resuspended in 200 μL of urea lysis buffer (6 M Urea; Sigma–Aldrich, Dorset, United Kingdom), 1 M ammonium bicarbonate (Fluka Chemicals Ltd. ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were grown at 37°C 6% CO2 and sub-confluent cultures were trypsinised with 0.25% Trypsin-EDTA (Sigma). For NHBE-A differentiation in Air Liquid Interface (ALI ...
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bioRxiv - Cell Biology 2023Quote: ... For each cell line 1.5 million cells were plated onto 6 cm plates and grown in DMEM (Sigma D5796) containing 10% FBS for 24 hours ...
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bioRxiv - Biochemistry 2023Quote: ... the ventricles were minced and dissociated into single cells by repeated sequential pancreatin (Sigma, [#8049-47-6, lot#SLBN2032V]) and collagenase II (Worthington ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were denatured (95 °C for 10 minutes) with 6×sample buffer [0.375 M Tris pH 6.8 (Sigma-Aldrich), 12% SDS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 polymer coverslip) or 6 (μ-Slide VI 0.4 ibiTreat: 1.5 polymer coverslip) channels were coated with fibronectin (SIGMA) for 1 hour and seeded with 100 000 or 50 000 cells per channel respectively ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were grown on 6-well plates previously coated overnight with a 0.1 mg/ml solution of poly-L-ornithine (Millipore), an important requirement to prevent cell detachment ...
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bioRxiv - Immunology 2023Quote: ... islets were incubated (37°C for 6 min) in a non-enzymatic cell dissociation solution (Sigma-Aldrich; #C5914-100ML) then vortexed to disrupt the islets.
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bioRxiv - Genetics 2023Quote: ... at 37°C in a 6-well plate and then 4mL of media was added (High glucose DMEM (Sigma), 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... we removed the zona pellucida by washing the oocytes in 4-6 drops of acidic Tyrode’s solution (Millipore Sigma) and rinsed them in 4-6 drops of CZB media with the corresponding drug(s ...
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bioRxiv - Cancer Biology 2023Quote: Five hundred A673 or SKES1 cells transduced with shRNAs targeting KCNN1 or shRNA non-targeting control were seeded in 6-well plates after 72 hours or 7 days of doxycycline treatment (0,5 µg/mL, Sigma). Cells were then incubated for 8 to 10 days under standard conditions (humidified incubator at 37°C and 5% CO2) ...
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bioRxiv - Biochemistry 2023Quote: ... snap-frozen eluates were thawed with the addition of 100 μL of 6 M guanidinium/HCl in 100 mM Tris/HCl pH 8.5 containing protease inhibitor cocktail (Sigma). Samples were washed with 6 M guanidine/HCl 100 mM Tris/HCl ...
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bioRxiv - Bioengineering 2023Quote: ... sGAG content was determined through a standard curve using chondroitin-6-sulphate (sodium salt from shark cartilage, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... Dye transfer from DiI-stained ALL cells cultured in transwells towards MSCs cultured in 6-well plates (Sigma Aldrich) was determined after 40 hours by flow cytometry ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then seeded on 6 mm diameter glass coverslips previously coated with 0.01% poly-L-lysine (Sigma, USA). 24h after seeding ...
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bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
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bioRxiv - Immunology 2023Quote: ... Final cell numbers were 6 x 107 cells/ml for large cultures (24-well size transwell plate, Millipore-Sigma) or 2 x 107 cells/ml for small cultures (96-well size transwell plate ...
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bioRxiv - Immunology 2023Quote: ... Final cell numbers were 6 x 107 cells/ml for large cultures (24-well size transwell plate, Millipore-Sigma) or 2 x 107 cells/ml for small cultures (96-well size transwell plate ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed with PBS and incubated with secondary antibodies for 2 h at RT in 2.5% BSA and 0.1% triton-X in PBS followed by counterstaining with 4’,6-diamidino-2-fenylindool (DAPI) (1µg/µl, Sigma, D9564) for 15 minutes at RT ...
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bioRxiv - Physiology 2023Quote: ... Unspecific antibody binding was blocked with 6% donkey serum or 4% goat serum (Sigma-Aldrich, St Louis, MO, USA), and 2.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... LSFM imaging was performed in ethyl cinnamate for index matching (Millipore Sigma, cat.no.: 112372, CAS number: 103-36-6) using the same imaging parameters.
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bioRxiv - Microbiology 2023Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre- soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were split into 6-well plates at a density of 0.5×106 cells/well and subjected to 2µg/mL puromycin (Sigma P8833) for selection of infected cells ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubating with the primary antibody (mouse monoclonal anti-acetylated tubulin, 6-11B-1, 1:1000, Sigma T6793) and then with the secondary antibody (goat anti-mouse IgG-Alexa Fluor 488 ...
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bioRxiv - Biochemistry 2023Quote: 293T cells were seeded into 6-well plates and transfected with replicon plasmid using Xtreme-Gene9 transfection reagent (Sigma). 24 hours post-transfection ...
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bioRxiv - Cell Biology 2023Quote: The CCl4 model of cirrhosis was prepared as previously described.23 Male C57Bl/6 mice were treated three times a week for 18 weeks with 0.1 ml of 40% CCl4 (Sigma) in olive oil by oral gavage.
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bioRxiv - Developmental Biology 2024Quote: ... and seeded at 8X105-1X106 cells per well in a 6 well plate coated with Matrigel (Millipore Sigma, CLS354277) 24 hours prior to differentiation treatment ...