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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Urotensin 2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were dried and reconstituted in 0.1% formic acid and desalted by C18 ziptip (EMD Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence of CHLI without transit peptide (1-46 amino acid residues) was cloned into pET32a (Novagen) and pGEX-4T-1 (GE healthcare ...
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bioRxiv - Biophysics 2022Quote: ... IP3R1 was eluted with 500 μM of T433 peptide and concentrated using a 100 kDa Amicon centricon (Millipore).
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bioRxiv - Biochemistry 2020Quote: ... and again with wash buffer before elution buffer (wash buffer with 300 ng 3x-FLAG peptide (Sigma Aldrich)) was added and incubated with beads overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Systems Biology 2021Quote: ... stringent wash were done with modified lysis buffer (250 mM NaCl + 20 µg/ml peptide FLAG (F3290 Sigma)) prior to elution with 200µg/ml peptide FLAG ...
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bioRxiv - Molecular Biology 2020Quote: ... Bound protein was eluted three times for 30 min by incubation with 0.3 mg/ml 3xFLAG peptide (Sigma) in BC100 at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was eluted using Buffer A with 150 mM NaCl and 200 μg/ml 3XFLAG peptide (Sigma). Peak fractions were pooled ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged protein was eluted at room temperature with 25 μl myc-peptide (0.5mg/mL in PBS, Sigma) for 15 minutes at 700 rpm mixing ...
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bioRxiv - Cell Biology 2021Quote: ... TiO2 beads (5010-21315, GL Sciences) were used to enrich phosphorylated peptide and homemade C8 (66882-U, Sigma) stageTip was used to trap the TiO2 beads for washes ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg of each peptide was injected into the abdominal cavity of each mouse along with 0.25 mL Complete Freund’s Adjuvant (Sigma). To enhance the immune response ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM TCEP) before elution using wash buffer supplemented with 0.25 mg/mL 3xFLAG peptide (F4799, Sigma-Aldrich). The eluent was then subjected to another round of affinity purification using anti-HA resin (26181 ...
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bioRxiv - Genetics 2020Quote: ... log phase cells (OD600~0.5 - 0.6) with bar1Δ were arrested in late G1 phase using 50 ng/ml alpha-factor peptide (Sigma) for ~120 minutes ...
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bioRxiv - Biophysics 2021Quote: ... Target proteins were eluted with 5 column volumes of wash buffer plus 200 μg/mL FLAG peptide (Sigma). The eluent was then applied to Strep-Tactin Sepharose (IBA ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were extracted using solutions of 50% and 80% acetonitrile (ACN) with 0.5% formic acid (Millipore), and the recovered solution dried down in a vacuum concentrator ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were extensively washed in lysis buffer and elution was carried out with 3×FLAG peptide (Sigma-Aldrich), HA peptide (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were resuspended in 35 µL of TBS buffer (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) supplemented with 0.5 µg/µL FLAG peptide (Sigma) and incubated at 4 °C for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluates containing digested peptides were separated from beads by centrifugation through a 0.45 µM PVDF filter column (Millipore) and cysteines were subsequently reduced and alkylated by addition of 5 mM TCEP and 10 mM CAA for 30 min at 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... GB1-TAZ2 binding to MITF peptides was determined utilising a polyhistidine monoclonal mouse antibody (1:5000, Sigma, H1029), incubated on the array for 4 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... high pH reversed-phase peptide prefractionation was performed with sulfonated divinylbenzene (CDS Empore™ SDB-RPS, Sigma-Aldrich) using a step gradient of increasing acetonitrile concentrations (0-60% ACN ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to binding of FLAG-tagged peptide species with anti-FLAG M2 magnetic beads (Sigma, cat # M8823). The purified FLAG-tagged peptides were harvested by eluting from the anti-FLAG M2 magnetic beads with 100 ng/µl 3xFLAG peptides.
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bioRxiv - Biochemistry 2023Quote: ... the proteins bound to the Flag antibodies were eluted using 400 µg/mL of 3xFlag peptide (Sigma, F4799) during 30 min at 4°C under agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides desalting was carried out using ZipTip U-C 18 spin columns (Cat. No. ZTC18M096, Millipore Corporation U.S.A.). The peptides were dried in a speed vac and then reconstituted with 0.1%Formic acid in water.
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bioRxiv - Biochemistry 2023Quote: ... Core complexes were eluted from anti-Flag resin with Flag buffer containing 250 μg/ml 3xFlag peptide (Sigma). Fractions containing protein were pooled and loaded on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides contained a C-term PEG-Biotin and were synthesized using a preloaded PEG-Biotin resin (Sigma-Aldrich). Peptides were cleaved from the resin using TFA / Water / Thioanisole / Triisopropylsilane / Phenol / Ethanedithiol – 82.5/0.5/0.5/0.25/0.25/0.25 and precipitated into cold diethyl ether ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT) before elution using wash buffer supplemented with 0.25 mg/ml 3xFLAG peptide (F4799, Sigma-Aldrich). Purified telomerase complex was verified by western blotting.
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bioRxiv - Biophysics 2024Quote: ... The Aβ (1–42) peptide (Stratech, A-1163, 0.5 mg) was initially dissolved in 100% HFIP (Sigma-Aldrich) at a concentration of μM ...
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bioRxiv - Microbiology 2024Quote: ... Stock solutions (1 mg/ml) were prepared by dissolving the peptides in 0.1% trifluoroacetic acid (TFA) (Sigma, Germany). The mass correctness and absence of degradation of the synthetic peptides were verified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS ...
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bioRxiv - Microbiology 2024Quote: Fifteen amino acid (aa) peptides overlapping 12 aa spanning the structural proteins of CHIKV were purchased from Sigma and were divided into 4 overlapping peptide pools ...
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bioRxiv - Cell Biology 2024Quote: ... Elution was done by adding either 20 μl of 0.2 mg/ml of 3xFLAG peptide (Sigma-Aldrich F4799) or 20 μl of 1x Strep-Tactin Elution Buffer (IBA 2-1042-025 ...
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bioRxiv - Microbiology 2024Quote: ... beads were resuspended in 35 µL TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) with 0.5 µg/µL FLAG peptide (Sigma) and incubated at room temperature for 15 min at 900 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... programed for uniform mixing as previously described at a controlled temperature and modified with online automated desalting.13 Proteins were denatured in a solution of 35% v/v 2-2-2 Trifluoroethanol (TFE, Sigma), 40 mM Dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2023Quote: ... mice were anesthetized by intraperitoneal injection with Avertin (2, 2, 2-Tribromoethanol, Sigma-Aldrich, St. Louis, MO, USA; catalog #T4). Cardiac perfusion was performed with 0.1 M phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the fluorescent dyes were removed by flowing 2 mL of the cleavage buffer containing 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; 646547, Sigma) with a 15-min incubation time in the flow chamber to cleave the disulfide bond linking the dyes to the readout probes ...
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bioRxiv - Immunology 2023Quote: ... T cells transduced with the anti-hCD19 CAR construct 42 were cultivated for additional 2 days in presence or without 2 mM 2-DG (Sigma) before adoptive transfer into MC38-hCD19 tumor-bearing mice.
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bioRxiv - Developmental Biology 2023Quote: ... The following factors were applied to experimental BPs in culture at the specified concentrations: 2-Deoxyglucose (2-DOG) 2 mM (SIGMA), Sodium Pyruvate (NaP ...
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bioRxiv - Biophysics 2023Quote: ... different amounts of freeze dried GelMA macromer were dissolved in DPBS containing 0.5% (w/v) 2-Hydroxy-4’-(2-hydroxyethoxy)-2- methylpropiophenone (Sigma-Aldrich) as photoinitiator ...
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bioRxiv - Immunology 2020Quote: ... for 2 days and then just IL-2 (20ng/ml) (Sigma-Aldrich) for a further 5 days ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissues were processed to 2’-2’-thio-diethanol (TDE, Sigma, 166782) for clearing and mounting ...
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bioRxiv - Immunology 2022Quote: ... 5 μM oligomycin and 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich) were injected to each well after 18 ...
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bioRxiv - Immunology 2021Quote: ... 2-Deoxy-D-glucose (2-DG) was obtained from Sigma Aldrich (D8375) and used at 0.32 mM ...
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bioRxiv - Genomics 2020Quote: ... Substrate solution (2 mM ABTS, Sigma A9941; 2 mM hydrogen peroxide, Sigma H1009 ...
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bioRxiv - Cell Biology 2021Quote: ... The last injection was supplemented with 2-deoxyglucose (2-DG, Sigma, D8375) to obtain the ECAR background value due to nonglycolytic acidification ...
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bioRxiv - Immunology 2019Quote: ... 2’-Deoxyadenosine (dAdo) and 2’-Deoxycytidine (dCyt) were purchased from Sigma-Aldrich Japan (Tokyo ...
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bioRxiv - Immunology 2021Quote: ... For the last 2 h 2 µg/ mL Brefeldin A (Sigma-Aldrich) was added ...
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bioRxiv - Immunology 2021Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 55μM 2-mercaptoethanol (2-ME) supplemented with 50 U/ml Benzonase (Sigma). Aliquots derived from the same donors were combined and all samples were enriched for T cells by removing CD14 and CD19 expressing cells using a column-based magnetic depletion approach according to the manufacturer’s recommendations (Miltenyi) ...
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bioRxiv - Immunology 2021Quote: ... cells were treated with 2-deoxyglucose (2-DG) 100 nM (Sigma, #D8375).