Labshake search
Citations for Millipore Sigma :
2001 - 2050 of 2423 citations for RhoD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Blocking was performed in 5% non-fat dry milk in PBS containing 0.2% Tween 20 (Sigma-Aldrich, St. Louis, Missouri, USA). Membranes were incubated in primary antibodies for 1-3 h at room temperature (20-25°C ...
-
bioRxiv - Immunology 2023Quote: ... in PBS for at least 30 min at 37 °C and permeabilized with PBS containing 0.1 % Triton-X for 5 minutes and blocked with blocking solution (3 % BSA in PBS) at RT for 30 minutes (all reagents from Sigma-Aldrich). Then ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells where thrice washed in PBS then incubated in blocking buffer (5% Donkey Serum (Jackson Immuno Research Labs 017-000-121, 1% BSA (Sigma A9647), 0.1% Triton X-100 (Sigma T8787 ...
-
bioRxiv - Neuroscience 2023Quote: ... Free-floating brain sections were then incubated for 1 hour at 4C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
-
bioRxiv - Biochemistry 2023Quote: ... then incubated for 1-2 h at room temperature in PBST with blocking agent (5 mg/mL Bovine Serum Albumin (Sigma Aldrich) for anti-Strep blots ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with anti-calbindin-D28k mouse monoclonal antibody (1:2000 dilution by the blocking solution, C9848, Millipore Sigma) at 4 °C overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were then incubated overnight at 4°C with the primary antibody diluted in blocking solution (5% goat and/or donkey serum in PBS, #G9023 and #D9663 Sigma-Aldrich). Cells were washed with complete PBS 3 times for 5min at RT and then incubated with the following secondary antibodies for 1h at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... Free-floating brain sections were then incubated for 1 hour at 4C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
-
bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
-
bioRxiv - Neuroscience 2023Quote: ... Coverslips were washed twice more with 0.01M PBS before blocking in 5% (v/v) normal donkey serum (NDS; catalogue # S30-100ml, Sigma Aldrich, UK) in 0.01M PBS (3% Tween-20 ...
-
bioRxiv - Genetics 2023Quote: ... The next morning membranes were washed once with blocking buffer for 5-10min and incubated with horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:10 000 in blocking buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Overnight primary antibody incubation was performed on a rocker in blocking solution containing the Anti-Tyrosine Hydroxylase (1/1000, Millipore, AB152) antibody ...
-
bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
-
bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
-
bioRxiv - Developmental Biology 2023Quote: ... all samples were incubated with Blocking One Histo (Nacalai Tesque) for 10 min at RT and 10% normal donkey serum (Sigma-Aldrich) in PBS/0.5% Triton X-100 with 5% Blocking One Histo for 30 min at RT.
-
bioRxiv - Biochemistry 2024Quote: ... Cells were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C: mouse anti-FLAG M2 (1:500; Sigma; F1804), rabbit anti-PEX14 (1:500 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked and permeabilized in protein-blocking buffer (DPBS with 2% BSA, 10% fish gelatin (cat#: G7041, Sigma-Aldrich), 0.2% Triton-X (cat# T9284 ...
-
bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
-
bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich] ...
-
bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were blocked with 1 drop of blocking solution provided with the Duolink® PLA Probes (DUO92002 and DUO92004, Sigma-Aldrich) for 1h at 37°C in a humid chamber ...
-
bioRxiv - Immunology 2021Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Sigma), developed with pNPP SigmaFAST substrate and absorbance was read with a 405nm – filter (Figure 3D-F).
-
bioRxiv - Molecular Biology 2021Quote: ... The beads were then washed three times for 5 min with mild EJC-Buffer before eluting twice with 22 μl of a 200 μg/ml dilution of FLAG-peptides (Sigma) in 1x TBS for 10 min at RT and 200 rpm each elution step ...
-
bioRxiv - Neuroscience 2020Quote: ... mice were administered murine MOG 35-55 (Johns Hopkins Peptide Synthesis Core Facility, Baltimore) in incomplete Freund’s adjuvant (Sigma-Aldrich) supplemented with heat-killed mycobacterium tuberculosis (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... encoding PPK with a C-terminal GAAEPEA peptide tag for affinity purification (105) between the NdeI and HindIII sites of plasmid pET-21b(+)(Novagen), was synthesized by GenScript.
-
bioRxiv - Molecular Biology 2020Quote: ... was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK) (Sigma-Aldrich). To generate CARD14-expressing vector (pTRE3G-Pur-3FLAG-CARD14wt ...
-
bioRxiv - Developmental Biology 2022Quote: A peptide corresponding to residues 1 – 159 of HyYap was expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
-
bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
-
bioRxiv - Immunology 2021Quote: ... The beads were then washed with IP buffer three times and incubated in FLAG-elution buffer (100 μg/mL FLAG peptides (Sigma), 50 mM Tris-HCl pH7.4 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and proteins were eluted in Laemmli buffer by boiling at 95°C for 5min for Western blot or with 3xFlag peptide (150 µg/ml, Sigma) for 1 hr at 4°C for HAT assay ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 2 hours at 4°(C) Immunocomplexes were washed 5× with S300 buffer and eluted by competition with 1x Flag peptide (500 ng/ul; SIGMA) 2 times for 30 min at 16 °C and then resuspended in Laemmli sample buffer ...
-
bioRxiv - Immunology 2019Quote: ... Commercial sources were used to obtain biotinylated CD16 (Acro Biosciences) and the biotinylated 19-mer adjacent loop peptides that overlap by 16 amino acids (Sigma). Loop peptide 1 is KDQQLLGIWGCSGKLICTT and loop peptide 2 is QLLGIWGCSGKLICTTAVP.
-
bioRxiv - Immunology 2020Quote: ... Beads were eluted three times at room temperature for 15 min each using Influenza Hemagglutinin (HA) peptide (Sigma Aldrich I2149).
-
bioRxiv - Cell Biology 2019Quote: ... Beads were washed extensively in lysis buffer and bound proteins eluted in 500 µL 100 µg/ml 3X FLAG peptide (Sigma) in 25 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Cancer Biology 2020Quote: ... were plated in 96-well plates (flat bottom, Costar) and pulsed with the indicated amounts of Melan-A peptides (ELAGIGILTV, Sigma) for 2 hours at 37C ...
-
bioRxiv - Immunology 2021Quote: ... plated at 2 × 106 cells/ml in cDMEM and rested overnight at 37°C in 7.5% CO2 following which BMDCs were stimulated overnight with Ag85B (20 µg/ml) (New England Peptide) and Zymosan (25 µg/ml) (Sigma) to induce maturation and activation ...
-
bioRxiv - Biochemistry 2021Quote: ... Peptides were extruded from the gel pieces by 15 minutes sonication and desalted using C18 ZipTips (Merch Millipore, Darmstadt, Germany), according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... were resolved on a 12.5% SDS gel and puromycin-labelled peptides were detected with anti-puromycin (1:5000 mouse anti-puromycin, clone 12D10; Sigma). Prior to antibody detection ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4°C) twice with 300 μL of Elution buffer (Lysis buffer A with 1 mg/mL 3 × FLAG peptide (Sigma)) ...
-
bioRxiv - Biochemistry 2020Quote: ... phosphorylation of peptides by the active Pro-directed kinase GST-CDK2/Cyclin A2 (100 ng purified from Sf9 cells, Sigma) was also confirmed in the presence or absence of the ATP competitive inhibitor ...
-
bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
-
bioRxiv - Neuroscience 2020Quote: ... These were formed by 24h incubation at 37°C of a 1mg/ml solution of Aβ 1-42 peptide (Sigma) in PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... elution was performed three times each using 20 μL of a 150 μg/μL stock of 3xFLAG peptides (Sigma Aldrich). Western blot analysis was done as previously described [7] ...
-
bioRxiv - Plant Biology 2020Quote: ... The coding sequence of PhS3-RNase (without signal peptide) as well as its six mutant forms described above were separately cloned into engineered pET-30a (Novagen) containing N-terminal SUMO tag to produce SUMO-His-tagged proteins ...
-
bioRxiv - Plant Biology 2020Quote: The coding sequences of PhS3-RNase (without signal peptide) and its six mutant forms were separately cloned into pMAl-c2x (Novagen) to generate MBP-tagged fusion proteins ...
-
bioRxiv - Microbiology 2020Quote: ... FLAG-tagged protein bound to RNA was eluted by incubation with 100 μL of FLAG elution solution (150 ng of 3X FLAG peptide per μL) (Sigma) for 30 min at 4°C ...
-
bioRxiv - Systems Biology 2020Quote: ... were used for binding assays with a constant amount of fluorescein isothiocyanate (FITC)-conjugated peptides (resuspended in 17 mM Tris-HCl pH 8, 100 mM NaCl and 0.5% Brij L23 (Millipore Sigma), peptides final concentration of 10 nM) ...
-
bioRxiv - Microbiology 2021Quote: ... Aminopeptidase activity was assessed by fluorescence assays using the fluorogenic peptide L-leucine-7-amido-4 methylcoumarin hydrochloride (Sigma L2145) as a substrate as previously described (19) ...
-
bioRxiv - Neuroscience 2019Quote: ... Supernatants were immediately used for measurement of proteasome peptidase activity by cleavage of the fluorogenic peptide N-Suc-LLVY-MCA (Sigma), enzymatic activity were acquired for 2h ...