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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Recombinant Human REG4 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... was cloned as 10× His-MBP-SUMO-NUP153 (or NSP1)-SNAP constructs (Figure S1) into a pET-28a-derived vector (Novagen) and expressed in BL21-Gold (DE3) ...
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bioRxiv - Molecular Biology 2020Quote: ... Trx-His(6)-Δlinker-hSLAP2 or Trx-His(6)-mSLAP were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% (v/v) Tween 20) containing 5% (w/v) BSA for 1 hour and then incubated with anti-His (Sigma) or anti-GFP antibody (Clontech ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant supplemented with final 10 mM imidazole was incubated with His-Select Nickel Affinity Gel (P6611, Lot No. 110M5166, SIGMA) for 1.5 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The filtered lysate was then incubated for 30 min with 1 ml of equilibrated HIS-Select HF Ni-affinity gel (Sigma) and loaded onto a gravity flow column ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation at 250,000 g for 30 min and the supernatant was incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Plant Biology 2023Quote: The auto activity of yeast baits on Sc-His due to minimal HIS3 expression was determined by spotting them on Sc-His media containing 0-30 mM 3-Amino-1,2,4-triazole (3-AT) (A8056, Sigma-Aldrich, USA). The minimum concentration of 3-AT that completely inhibits the growth of the bait was considered for the Y1H assay (20mM for pdistal ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Plant Biology 2023Quote: ... bacteria were harvested by centrifugation and pellets were resuspended in 5 ml of binding buffer (His-bind buffer kit (Millipore), proteins were extracted using a Constant cell disrupter system (Constant Systems ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen cell pellets were thawed and then lysed using ice cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 (Sigma), 10 mM NaCl (Sigma) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Microbiology 2023Quote: ... 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a fast protein liquid chromatography (FPLC ...
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bioRxiv - Microbiology 2023Quote: ... pET28a-His-DNTTIP1 has been generated by in frame insertion of DNTTIP1 cDNA into BamH1/Xho1 sites of pET28a(+) (Novagen, kindly provided by Dr ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by four washes with 200 μl of incubation buffer and incubation with 50 μl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... A soluble extract was prepared by centrifugation at 14,000 × g for 30 min and applied to Ni-NTA His-bind Resin (Millipore Sigma) equilibrated with binding buffer (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked using 5% nonfat milk and probed with anti-poly-His primary antibody (cat. no. H1029; Sigma-Aldrich) at 1:3,000 dilution or anti- GST primary antibody (cat ...
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bioRxiv - Biochemistry 2024Quote: ... and the final purified SbSIP was concentrated from the flow-through of a second passage through the His-trap column using an Amicon Ultra Centrifugal Filter (Millipore) with a 30 kDa cutoff ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in PBS with 2% HI-FBS (Hyclone, #SH30910.03) containing 1 µg/mL DAPI (Sigma Aldrich, #D9542) to detect dead cells ...
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bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Pathology 2023Quote: ... adiponectin levels were measured by enzyme-linked immunosorbent assays (Human Chemerin ELISA kit, Biovendor, Brno, Czech Republic; Human Resistin ELISA kit, Millipore Sigma; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μg/ml prolactin (mouse recombinant for qPCR, western blot, immunohistochemistry and contraction control; Sigma or Peprotech) or sheep pituitary prolactin for time-lapse and confocal imaging ...
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bioRxiv - Immunology 2019Quote: ... A subset of mice was treated with IP with recombinant DNase I (20 mg/kg, Sigma-Aldrich) 2 hours after LPS injection24 ...
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bioRxiv - Microbiology 2021Quote: ... enzymatic activity of recombinant CynD was measured at pH 8.0 using the Ammonia Assay Kit (Sigma-Aldrich). A concentration of 500 nM of CynD was used in all reactions with the following cyanide concentrations ...
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bioRxiv - Microbiology 2021Quote: ... Digestion of extended E34TSP was done according to the recombinant Enterokinase user protocol TB150 Rev.C 0107(Novagen). The extent of proteolytic activity on our TSP was calibrated via SDS PAGE analysis.
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bioRxiv - Microbiology 2021Quote: ... Viral titers were calculated from a standard curve generated using recombinant reverse transcriptase (Millipore catalog no. 382129).
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bioRxiv - Synthetic Biology 2020Quote: ... Sf9 cells were transfected with recombinant bacmid DNA using Escort™ IV Transfection Reagent (Sigma, Cat # L3287) in 6 well plates ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...