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Citations for Millipore Sigma :
2001 - 2050 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The FLAG-tagged protein-DNA complex was purified via affinity chromatography using anti-FLAG M2 affinity gel (Sigma-Aldrich, Taufkirchen, Germany). The purified protein-DNA complexes and the control were incubated at 95 °C for 40 min for reversal of the crosslink ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of lysates of hTERT-RPE1 cells stably expressing HA-tagged WDR60 or WDR34 using anti-HA agarose beads (Sigma-Aldrich). Lysis buffer containing 50 mM Tris/HCl ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Molecular Biology 2022Quote: ... cell surface HS level was examined after staining with anti-HS antibody (10E4, amsbio) at 5 μg/ml and FLAG-tagged enzymes were detected by using anti-FLAG® M2 antibody (Sigma), at 2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged protein was precipitated with 20 μl anti-V5 beads (Anti-V5 Agarose Affinity Gel, Cat.- No. A7345, Sigma-Aldrich) rotating on a wheel for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... resolved by SDS-PAGE and immunoblotted for the presence of the HA- tagged effector using α-HA antibody (H9658; Sigma-Aldrich) and α-HA-HRP (3F10 ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... MBP- and GST-tagged proteins were purified by gel filtration on a Superdex 200 column (Sigma-Aldrich Cat# GE17-5175-01) and concentrated using a 30K Amicon filter.
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bioRxiv - Biochemistry 2023Quote: Hexahistidine tagged hMDC1 BRCT (residues: 1891 – 2089) was cloned into pET47b vector and expressed in Rosetta™ 2(DE3) cell lines (Novagen). Cells in 1L Luria Broth (LB ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA-tagged antibody conjugated with a PLUS oligonucleotide was prepared using the Duolink In Situ Probemaker PLUS (DUO92009; Sigma-Aldrich). The specimens were washed twice with wash buffer A and were subsequently incubated with Duolink ligation solution for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged Af1521 macrodomain beads were produced in-house using BL21(DE3) bacteria and coupled to glutathione Sepharose 4B beads (Sigma-Aldrich), essentially as previously described ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated with HRP-tagged secondary antibodies and protein bands were detected using ECL Prime western blotting system (Catalog No. GERPN2232, Millipore Sigma). Protein band density was measured using ImageJ 1.52a ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS + 1% P/S + 0.1 μM phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich) + 1μM Ionomycin (Sigma Alrich ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilization and blocking of samples was performed in blocking solution (10% FCS, 0.3% Saponin (47036, Sigma-Aldrich) in PBS 1x ...
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bioRxiv - Immunology 2019Quote: ... in FACS buffer (PBS supplemented with 2% FCS and 2 mM (Ethylenedinitrilo)tetraacetic acid (EDTA) (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were then washed 8 times and avidin-peroxidase (2.5 μg/mL in PBS / 10 % FCS. Sigma) was added for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... a 15-minute chase was performed with normal medium (0.5% FCS) supplemented with 10 µM thymidine (Sigma) to remove unincorporated EdU and cells were fixed and permeabilized with 3.7% FA and 0.5% Triton X-100 for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in FluroDishes (World Precision) and imaged in DMEM supplemented with 10% FCS (Sigma-Aldrich), 100U/ml penicillin and 100μg/ml puromycin (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... the spinal cord was placed in a plexiglass tube and immersed in fluorinert (FC-40 Sigma Aldrich) to reduce background signal ...
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bioRxiv - Microbiology 2021Quote: ... Procyclic forms (PCF; strain 427) were cultured in modified SDM-79 with 10 % heat-inactivated FCS (Sigma) at 27°C ...
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bioRxiv - Developmental Biology 2022Quote: ... A trapped bead was manually manipulated by a motorized XY stage (BIOS-225T and FC-101G, Sigma), and contact of the bead with the cilium during oscillation for 1.5 h was monitored by ciliary GCaMP6 or mCherry fluorescence imaging or bright-field imaging of the bead ...
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bioRxiv - Cancer Biology 2019Quote: ... one cells have adhered remove culture medium and replace with diluted lentiviral media (500µl lentiviral media, 1.5ml DMEM, 10% FCS, 5% PenStrep, 5-10µg Polybrene (Sigma)) ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and resuspended in RPMI supplemented with 10% FCS and 1% penicillin streptomycin (Sigma Aldrich) (complete media ...
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bioRxiv - Pathology 2020Quote: ... stimulated for 24 hours in 10% FCS DMEM with LPS (1 µg/ml, Salmonella Minnesota; Sigma-Aldrich), BLP (Pam3Cys-Ser-(Lys)4 ...
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bioRxiv - Systems Biology 2019Quote: ... Subsequently, cells were washed two times with a washing solution (5% FCS, 0.05% BSA (Sigma-Aldrich; A2058), 0.02% NaN3(Carl Roth GmbH & Co ...
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bioRxiv - Developmental Biology 2021Quote: ... the embryos were harvested in sterile medium (10% FCS (Hyclone) and 1% pen/strep in PBS (Sigma)) ...
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bioRxiv - Immunology 2021Quote: ... Medium was replaced every 3 days thereafter with fresh R+ containing FCS (10% v/v; Sigma-Aldrich). Antigen-specific CD8+ T cells were characterized via flow cytometry on day 10.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Coding region of RBD-Fc was subcloned into a modified pCGS3 (Merck/formally known as Sigma-Aldrich) for glutamine selection in CHOZN GS-/- cells ...
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bioRxiv - Immunology 2022Quote: ... 1*105 cells in 200μl/well complete T-cell medium (RPMI1640 supplemented with 10% FCS (Sigma/Biowest), antibiotics [PenStrep] ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM glutamine and 10% dialyzed FCS after addition of 25 mM of either 12C-glucose (Sigma) or U-13C-glucose (Cambridge Isotopes ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% (v/v) heat-inactived fetal calf serum (FCS, Sigma-Aldrich, Saint Louis, MO, USA), 400 µg/ml G418 (Carl Roth ...
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bioRxiv - Immunology 2023Quote: ... density centrifugation and cryopreserved in heat-inactivated foetal calf serum (FCS) supplemented with 10% DMSO (Sigma-Aldrich) in liquid nitrogen ...
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bioRxiv - Immunology 2022Quote: ... 7 – 13×106 cells were washed in PBS with 2% FCS before incubation in 50nM dasatinib (Sigma) at 37°C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... including Phosphate Buffered Saline (PBS) and Fetal Calf Serum (FCS) were purchased from Sigma Aldrich (Gillingham, UK), except for blastocidin which was obtained from Gibco™ (MA ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with 0.8 µg/mL HRP-conjugated goat anti-mouse IgG (Fc specific, Sigma-Aldrich). Visualization of protein bands was performed with Pierce™ ECL Plus Western Blotting Substrate (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... elution profiles and sample purity were confirmed by Western blot with antibodies against His tag (Sigma, H1029, lot 033m4785) and Strep tag (IBA ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 3×106 co-transformants was initially screened for growth on synthetic defined media (SD)-Leu-/ Trp-/ Ura-/ His- media containing 20mM of 3-amino-1,2,4-triazole (3-AT, Sigma-Aldrich). Plasmids were isolated from the potential positive colonies ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Neuroscience 2019Quote: ... Adult zebrafish were assayed for response to a mixture of 8 amino acids (Ala, Cys, His, Lys, Met, Phe, Trp, and Val; 0.1 mM each in distilled water; Sigma). No stimulation was provided for the first 5 mins following which the amino acid mix was introduced in one side of the tank at the rate of 1 ml/min using a syringe pump ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...