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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Recombinant Human CFHR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was collected and total protein content determined with a BCA Protein Assay (Sigma, Poole, UK). A total of 20μg protein extract for each sample was separated by electrophoresis on Nu-PAGE™ 4-12% ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was retrieved and protein concentration was measured by BCA protein assay (Novagen, (Smith et al. 1985). Protein concentrations were adjusted in all the samples to 800 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: Protein—protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were extracted from the conditioned medium using the protein precipitation kit (#2100, Millipore Sigma, Burlington, MA) following the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2022Quote: Protein-protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells and large quantities of His6 tagged recombinant Nbs were purified with 1ml of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) following periplasmic expression through osmotic shock ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was cut in two (one half for the commercial and one half for the recombinant anti-FLAG™-M2) and each half was probed with either commercial (Sigma) or recombinant anti-FLAG™-M2 at 1 µg/mL in PBS for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant LymCBP HAT domain (200 ng) were incubated with the histone H1 purified from calf thymus (Sigma-Aldrich, 1 μg) and 7.4 kBq of [14C]-acetyl-CoA (Moravek ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...
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bioRxiv - Neuroscience 2021Quote: ... αSyn oligomers were produced by incubating recombinant αSyn to a final concentration of 20 µM with 300 µM DOPAL or dopamine (Sigma-Aldrich) in PBS ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Cell Biology 2021Quote: ... 5a), recombinant mEGFP(−7e) or scGFP(+7e) were dissolved in injection buffer (50 mM K-HEPES, pH 7.4 (Sigma-Aldrich, H3375), 25% glycerol (Applichem ...
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Neuroscience 2024Quote: ... lateral and medial) were each injected with 10 μl of either PBS or recombinant mouse GDNF (10 μg/ml, Sigma-Aldrich) using 31-gauge insulin syringes without damaging any innervating nerves ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded on 6-well cell culture plate wells at a density of ∼2 x 105 cells/well with 2 mL of the culture medium containing 103 units/mL ESGRO Recombinant Mouse Leukemia inhibitory factor (LIF) (Millipore #ESG1107) and 2i (1.0 µM PD0325901 Toris #4423 and 3.0 µM CHIR99021 Wako #038-23101) ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... stable PDA cell lines were generated by infecting 70% confluent cultures with the recombinant lentiviral vectors with 10 mg/mL polybrene (TR-1003, Sigma-Aldrich) twice over 2 consecutive days ...
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bioRxiv - Biochemistry 2024Quote: ... were mixed with 60 ng of recombinant HA-BACH1 in 21 μl of a reaction buffer containing 10 μg of bovine ubiquitin (Sigma-Aldrich), 2 mM ATP ...
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bioRxiv - Cancer Biology 2021Quote: ... Human primary fibroblasts (HFFF2) were treated with 50μM of Etoposide (Sigma-Aldrich) for 2 days and cells collected at day 7 or treated with 1μM Palbociclib for 7 days ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma samples were depleted once each using human IgY14 LC10 (Sigma S5074) and human Supermix LC5 (Sigma S5324 ...
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bioRxiv - Cell Biology 2020Quote: All the substrates were functionalized with human plasma fibronectin (FN, Sigma-Aldrich). After sterilizing with UV for 30 min ...
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bioRxiv - Immunology 2021Quote: ... and 10% human serum (heat-inactivated, sterile-filtered, male AB plasma; SIGMA). Human IL-2 (TECIN™ teceleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human islets were washed in 1x PBS and resuspended in Accutase (Sigma) dissociation solution for 3 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with rabbit anti-human SOX9 primary antibody (AB5535, Millipore) in the blocking solution for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... for Mouse 448 and human experiments or 1 mg/ml Hoechst (Sigma) for the mouse atlas experiments ...
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bioRxiv - Biophysics 2022Quote: ... consisting of OF-medium complemented with 10μg/ml human transferrin (Sigma-Aldrich), 20nM human insulin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Macrophage colony-stimulating factor (M-CSF, human) was purchased from Millipore Sigma, St ...
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bioRxiv - Immunology 2022Quote: Human dermal fibroblasts at passage 20 were cultured in complete DMEM (Sigma) supplemented with 1% pen/step (Sigma ...
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bioRxiv - Genomics 2020Quote: ... Purified rabbit polyclonal antibody against human TCF21 (HPA013189) was purchased from Sigma. Recombinant human TGFβ (AF-100-21C ...
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bioRxiv - Cell Biology 2019Quote: ... followed by 7.5 IU human chorionic gonadotropin (Cat.# CG5-1VL, Sigma-Aldrich) 46-48 h later ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 ng human gDNA from buffy coat (Sigma Aldrich, St. Louis, MO) was added in all experiments except those using clinical isolates ...
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bioRxiv - Immunology 2021Quote: ... using the Non-Human Primate Cytokine MILLIPLEX map 23-plex kit (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 5% CO2 for 1 hour in human tubular fluid (HTF, EMD Millipore) at 2.0 × 106 cells/ml and co-incubated with oocytes at 2.0 × 105 sperm/ml ...
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bioRxiv - Bioengineering 2021Quote: ... and 5 ng/mL luteinizing hormone from human pituitary (LH, Sigma, L6420), with media changes every other day ...
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bioRxiv - Bioengineering 2021Quote: ... and 5 ng/mL luteinizing hormone from human pituitary (LH, Sigma, L6420), with media changes every other day ...
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bioRxiv - Bioengineering 2021Quote: ... A 100 nM CGRP (human α-CGRP, Sigma-Aldrich, St. Louis, MO) solution was prepared in aCSF ...
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bioRxiv - Bioengineering 2021Quote: ... and human plasminogen (SRP6518) were purchased from Sigma-Aldrich (St. Louis, MO). Ethyl acetate (142-89-2) ...
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bioRxiv - Bioengineering 2020Quote: ... and 15 μl/ml human albumin (Sigma-Aldrich, St. Louis, MO, USA) in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biophysics 2021Quote: ... human ovarian cancer cell line A2780 was purchased from Sigma Aldrich (#93112519), whereas THP-1 monocyte cell line was supplied by a third part ...
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bioRxiv - Cell Biology 2021Quote: ... Insulin solution human was obtained from Sigma (Sigma Aldrich, Dorset, UK, I9278).
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bioRxiv - Microbiology 2020Quote: ... blots were reprobed with mouse anti-human tubulin (1:3000, Sigma T5168). SUMO1 smears above 90 kDa (excluding therefore the high intensity RanGAP1 bands present at 80 kDa ...
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bioRxiv - Microbiology 2020Quote: ... 10ng/ml human KGF (novoprotein, CM88) and 20 µM DAPT (Sigma, D5942) from day 14 to day 21 ...
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bioRxiv - Systems Biology 2021Quote: ... were coated with 12.5 μg/ml human fibronectin (Sigma Aldrich, Darmstadt, Germany) for 1 h at RT ...